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Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Applied and Environmental Microbiology, 82, 2016, 5, S. 1577-1585 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
|
| Schlagwörter: |
| author_facet |
Zhou, Jia-Yu Yuan, Jie Li, Xia Ning, Yi-Fan Dai, Chuan-Chao Zhou, Jia-Yu Yuan, Jie Li, Xia Ning, Yi-Fan Dai, Chuan-Chao |
|---|---|
| author |
Zhou, Jia-Yu Yuan, Jie Li, Xia Ning, Yi-Fan Dai, Chuan-Chao |
| spellingShingle |
Zhou, Jia-Yu Yuan, Jie Li, Xia Ning, Yi-Fan Dai, Chuan-Chao Applied and Environmental Microbiology Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
zhou, jia-yu |
| spelling |
Zhou, Jia-Yu Yuan, Jie Li, Xia Ning, Yi-Fan Dai, Chuan-Chao 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.03434-15 <jats:title>ABSTRACT</jats:title> <jats:p> Oxygenous terpenoids are active components of many medicinal plants. However, current studies that have focused on enzymatic oxidation reactions cannot comprehensively clarify the mechanisms of oxygenous terpenoid synthesis and diversity. This study shows that an endophytic bacterium can trigger the generation of reactive oxygen species (ROS) that directly increase oxygenous sesquiterpenoid content and diversity in <jats:named-content content-type="genus-species">Atractylodes lancea</jats:named-content> . <jats:named-content content-type="genus-species">A. lancea</jats:named-content> is a famous but endangered Chinese medicinal plant that contains abundant oxygenous sesquiterpenoids. Geo-authentic <jats:named-content content-type="genus-species">A. lancea</jats:named-content> produces a wider range and a greater abundance of oxygenous sesquiterpenoids than the cultivated herb. Our previous studies have shown the mechanisms behind endophytic promotion of the production of sesquiterpenoid hydrocarbon scaffolds; however, how endophytes promote the formation of oxygenous sesquiterpenoids and their diversity is unclear. After colonization by <jats:named-content content-type="genus-species">Pseudomonas fluorescens</jats:named-content> ALEB7B, oxidative burst and oxygenous sesquiterpenoid accumulation in <jats:named-content content-type="genus-species">A. lancea</jats:named-content> occur synchronously. Treatment with exogenous hydrogen peroxide (H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> ) or singlet oxygen induces oxidative burst and promotes oxygenous sesquiterpenoid accumulation <jats:named-content content-type="genus-species">in planta</jats:named-content> . Conversely, pretreatment of plantlets with the ROS scavenger ascorbic acid significantly inhibits the oxidative burst and oxygenous sesquiterpenoid accumulation induced by <jats:named-content content-type="genus-species">P. fluorescens</jats:named-content> ALEB7B. Further <jats:italic>in vitro</jats:italic> oxidation experiments show that several oxygenous sesquiterpenoids can be obtained from direct oxidation caused by H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> or singlet oxygen. In summary, this study demonstrates that endophytic bacterium-triggered ROS can directly oxidize oxygen-free sesquiterpenoids and increase the oxygenous sesquiterpenoid content and diversity in <jats:named-content content-type="genus-species">A. lancea</jats:named-content> , providing a novel explanation of the mechanisms of oxygenous terpenoid synthesis <jats:named-content content-type="genus-species">in planta</jats:named-content> and an essential complementarity to enzymatic oxidation reactions. </jats:p> Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea Applied and Environmental Microbiology |
| doi_str_mv |
10.1128/aem.03434-15 |
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Online Free |
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American Society for Microbiology, 2016 |
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American Society for Microbiology, 2016 |
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0099-2240 1098-5336 |
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zhou2016endophyticbacteriumtriggeredreactiveoxygenspeciesdirectlyincreaseoxygenoussesquiterpenoidcontentanddiversityinatractylodeslancea |
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2016 |
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American Society for Microbiology |
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Applied and Environmental Microbiology |
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49 |
| title |
Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea |
| title_unstemmed |
Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea |
| title_full |
Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea |
| title_fullStr |
Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea |
| title_full_unstemmed |
Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea |
| title_short |
Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea |
| title_sort |
endophytic bacterium-triggered reactive oxygen species directly increase oxygenous sesquiterpenoid content and diversity in atractylodes lancea |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.03434-15 |
| publishDate |
2016 |
| physical |
1577-1585 |
| description |
<jats:title>ABSTRACT</jats:title>
<jats:p>
Oxygenous terpenoids are active components of many medicinal plants. However, current studies that have focused on enzymatic oxidation reactions cannot comprehensively clarify the mechanisms of oxygenous terpenoid synthesis and diversity. This study shows that an endophytic bacterium can trigger the generation of reactive oxygen species (ROS) that directly increase oxygenous sesquiterpenoid content and diversity in
<jats:named-content content-type="genus-species">Atractylodes lancea</jats:named-content>
.
<jats:named-content content-type="genus-species">A. lancea</jats:named-content>
is a famous but endangered Chinese medicinal plant that contains abundant oxygenous sesquiterpenoids. Geo-authentic
<jats:named-content content-type="genus-species">A. lancea</jats:named-content>
produces a wider range and a greater abundance of oxygenous sesquiterpenoids than the cultivated herb. Our previous studies have shown the mechanisms behind endophytic promotion of the production of sesquiterpenoid hydrocarbon scaffolds; however, how endophytes promote the formation of oxygenous sesquiterpenoids and their diversity is unclear. After colonization by
<jats:named-content content-type="genus-species">Pseudomonas fluorescens</jats:named-content>
ALEB7B, oxidative burst and oxygenous sesquiterpenoid accumulation in
<jats:named-content content-type="genus-species">A. lancea</jats:named-content>
occur synchronously. Treatment with exogenous hydrogen peroxide (H
<jats:sub>2</jats:sub>
O
<jats:sub>2</jats:sub>
) or singlet oxygen induces oxidative burst and promotes oxygenous sesquiterpenoid accumulation
<jats:named-content content-type="genus-species">in planta</jats:named-content>
. Conversely, pretreatment of plantlets with the ROS scavenger ascorbic acid significantly inhibits the oxidative burst and oxygenous sesquiterpenoid accumulation induced by
<jats:named-content content-type="genus-species">P. fluorescens</jats:named-content>
ALEB7B. Further
<jats:italic>in vitro</jats:italic>
oxidation experiments show that several oxygenous sesquiterpenoids can be obtained from direct oxidation caused by H
<jats:sub>2</jats:sub>
O
<jats:sub>2</jats:sub>
or singlet oxygen. In summary, this study demonstrates that endophytic bacterium-triggered ROS can directly oxidize oxygen-free sesquiterpenoids and increase the oxygenous sesquiterpenoid content and diversity in
<jats:named-content content-type="genus-species">A. lancea</jats:named-content>
, providing a novel explanation of the mechanisms of oxygenous terpenoid synthesis
<jats:named-content content-type="genus-species">in planta</jats:named-content>
and an essential complementarity to enzymatic oxidation reactions.
</jats:p> |
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| description | <jats:title>ABSTRACT</jats:title> <jats:p> Oxygenous terpenoids are active components of many medicinal plants. However, current studies that have focused on enzymatic oxidation reactions cannot comprehensively clarify the mechanisms of oxygenous terpenoid synthesis and diversity. This study shows that an endophytic bacterium can trigger the generation of reactive oxygen species (ROS) that directly increase oxygenous sesquiterpenoid content and diversity in <jats:named-content content-type="genus-species">Atractylodes lancea</jats:named-content> . <jats:named-content content-type="genus-species">A. lancea</jats:named-content> is a famous but endangered Chinese medicinal plant that contains abundant oxygenous sesquiterpenoids. Geo-authentic <jats:named-content content-type="genus-species">A. lancea</jats:named-content> produces a wider range and a greater abundance of oxygenous sesquiterpenoids than the cultivated herb. Our previous studies have shown the mechanisms behind endophytic promotion of the production of sesquiterpenoid hydrocarbon scaffolds; however, how endophytes promote the formation of oxygenous sesquiterpenoids and their diversity is unclear. After colonization by <jats:named-content content-type="genus-species">Pseudomonas fluorescens</jats:named-content> ALEB7B, oxidative burst and oxygenous sesquiterpenoid accumulation in <jats:named-content content-type="genus-species">A. lancea</jats:named-content> occur synchronously. Treatment with exogenous hydrogen peroxide (H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> ) or singlet oxygen induces oxidative burst and promotes oxygenous sesquiterpenoid accumulation <jats:named-content content-type="genus-species">in planta</jats:named-content> . Conversely, pretreatment of plantlets with the ROS scavenger ascorbic acid significantly inhibits the oxidative burst and oxygenous sesquiterpenoid accumulation induced by <jats:named-content content-type="genus-species">P. fluorescens</jats:named-content> ALEB7B. Further <jats:italic>in vitro</jats:italic> oxidation experiments show that several oxygenous sesquiterpenoids can be obtained from direct oxidation caused by H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> or singlet oxygen. In summary, this study demonstrates that endophytic bacterium-triggered ROS can directly oxidize oxygen-free sesquiterpenoids and increase the oxygenous sesquiterpenoid content and diversity in <jats:named-content content-type="genus-species">A. lancea</jats:named-content> , providing a novel explanation of the mechanisms of oxygenous terpenoid synthesis <jats:named-content content-type="genus-species">in planta</jats:named-content> and an essential complementarity to enzymatic oxidation reactions. </jats:p> |
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| spelling | Zhou, Jia-Yu Yuan, Jie Li, Xia Ning, Yi-Fan Dai, Chuan-Chao 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.03434-15 <jats:title>ABSTRACT</jats:title> <jats:p> Oxygenous terpenoids are active components of many medicinal plants. However, current studies that have focused on enzymatic oxidation reactions cannot comprehensively clarify the mechanisms of oxygenous terpenoid synthesis and diversity. This study shows that an endophytic bacterium can trigger the generation of reactive oxygen species (ROS) that directly increase oxygenous sesquiterpenoid content and diversity in <jats:named-content content-type="genus-species">Atractylodes lancea</jats:named-content> . <jats:named-content content-type="genus-species">A. lancea</jats:named-content> is a famous but endangered Chinese medicinal plant that contains abundant oxygenous sesquiterpenoids. Geo-authentic <jats:named-content content-type="genus-species">A. lancea</jats:named-content> produces a wider range and a greater abundance of oxygenous sesquiterpenoids than the cultivated herb. Our previous studies have shown the mechanisms behind endophytic promotion of the production of sesquiterpenoid hydrocarbon scaffolds; however, how endophytes promote the formation of oxygenous sesquiterpenoids and their diversity is unclear. After colonization by <jats:named-content content-type="genus-species">Pseudomonas fluorescens</jats:named-content> ALEB7B, oxidative burst and oxygenous sesquiterpenoid accumulation in <jats:named-content content-type="genus-species">A. lancea</jats:named-content> occur synchronously. Treatment with exogenous hydrogen peroxide (H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> ) or singlet oxygen induces oxidative burst and promotes oxygenous sesquiterpenoid accumulation <jats:named-content content-type="genus-species">in planta</jats:named-content> . Conversely, pretreatment of plantlets with the ROS scavenger ascorbic acid significantly inhibits the oxidative burst and oxygenous sesquiterpenoid accumulation induced by <jats:named-content content-type="genus-species">P. fluorescens</jats:named-content> ALEB7B. Further <jats:italic>in vitro</jats:italic> oxidation experiments show that several oxygenous sesquiterpenoids can be obtained from direct oxidation caused by H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> or singlet oxygen. In summary, this study demonstrates that endophytic bacterium-triggered ROS can directly oxidize oxygen-free sesquiterpenoids and increase the oxygenous sesquiterpenoid content and diversity in <jats:named-content content-type="genus-species">A. lancea</jats:named-content> , providing a novel explanation of the mechanisms of oxygenous terpenoid synthesis <jats:named-content content-type="genus-species">in planta</jats:named-content> and an essential complementarity to enzymatic oxidation reactions. </jats:p> Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea Applied and Environmental Microbiology |
| spellingShingle | Zhou, Jia-Yu, Yuan, Jie, Li, Xia, Ning, Yi-Fan, Dai, Chuan-Chao, Applied and Environmental Microbiology, Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea |
| title_full | Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea |
| title_fullStr | Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea |
| title_full_unstemmed | Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea |
| title_short | Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea |
| title_sort | endophytic bacterium-triggered reactive oxygen species directly increase oxygenous sesquiterpenoid content and diversity in atractylodes lancea |
| title_unstemmed | Endophytic Bacterium-Triggered Reactive Oxygen Species Directly Increase Oxygenous Sesquiterpenoid Content and Diversity in Atractylodes lancea |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.03434-15 |