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Phosphorothioated DNA Is Shielded from Oxidative Damage
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| Zeitschriftentitel: | Applied and Environmental Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , |
| In: | Applied and Environmental Microbiology, 85, 2019, 8 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Microbiology
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| Schlagwörter: |
| author_facet |
Pu, Tianning Liang, Jingdan Mei, Zhiling Yang, Yan Wang, Jialiang Zhang, Wei Liang, Wei-Jun Zhou, Xiufen Deng, Zixin Wang, Zhijun Pu, Tianning Liang, Jingdan Mei, Zhiling Yang, Yan Wang, Jialiang Zhang, Wei Liang, Wei-Jun Zhou, Xiufen Deng, Zixin Wang, Zhijun |
|---|---|
| author |
Pu, Tianning Liang, Jingdan Mei, Zhiling Yang, Yan Wang, Jialiang Zhang, Wei Liang, Wei-Jun Zhou, Xiufen Deng, Zixin Wang, Zhijun |
| spellingShingle |
Pu, Tianning Liang, Jingdan Mei, Zhiling Yang, Yan Wang, Jialiang Zhang, Wei Liang, Wei-Jun Zhou, Xiufen Deng, Zixin Wang, Zhijun Applied and Environmental Microbiology Phosphorothioated DNA Is Shielded from Oxidative Damage Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| author_sort |
pu, tianning |
| spelling |
Pu, Tianning Liang, Jingdan Mei, Zhiling Yang, Yan Wang, Jialiang Zhang, Wei Liang, Wei-Jun Zhou, Xiufen Deng, Zixin Wang, Zhijun 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.00104-19 <jats:p> DNA phosphorothioation has been reported in many bacteria. These PT-hosting bacteria live in very different environments, such as the human body, soil, or hot springs. The physiological function of DNA PT modification is still elusive. A remarkable property of PT modification is that purified genomic PT DNA is susceptible to oxidative cleavage. Among the oxidants, hypochlorous acid and H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> are of physiological relevance for human pathogens since they are generated during the human inflammation response to bacterial infection. However, expression of PT genes in the catalase-deficient <jats:named-content content-type="genus-species">E. coli</jats:named-content> Hpx <jats:sup>−</jats:sup> strain restores H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> resistance. Here, we seek to solve this obvious paradox. We demonstrate that DndCDE-FeS is a short-lived catalase that binds tightly to PT DNA. It is thus possible that by docking to PT sites the catalase activity protects the bacterial genome against H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> damage. </jats:p> Phosphorothioated DNA Is Shielded from Oxidative Damage Applied and Environmental Microbiology |
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10.1128/aem.00104-19 |
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| title |
Phosphorothioated DNA Is Shielded from Oxidative Damage |
| title_unstemmed |
Phosphorothioated DNA Is Shielded from Oxidative Damage |
| title_full |
Phosphorothioated DNA Is Shielded from Oxidative Damage |
| title_fullStr |
Phosphorothioated DNA Is Shielded from Oxidative Damage |
| title_full_unstemmed |
Phosphorothioated DNA Is Shielded from Oxidative Damage |
| title_short |
Phosphorothioated DNA Is Shielded from Oxidative Damage |
| title_sort |
phosphorothioated dna is shielded from oxidative damage |
| topic |
Ecology Applied Microbiology and Biotechnology Food Science Biotechnology |
| url |
http://dx.doi.org/10.1128/aem.00104-19 |
| publishDate |
2019 |
| physical |
|
| description |
<jats:p>
DNA phosphorothioation has been reported in many bacteria. These PT-hosting bacteria live in very different environments, such as the human body, soil, or hot springs. The physiological function of DNA PT modification is still elusive. A remarkable property of PT modification is that purified genomic PT DNA is susceptible to oxidative cleavage. Among the oxidants, hypochlorous acid and H
<jats:sub>2</jats:sub>
O
<jats:sub>2</jats:sub>
are of physiological relevance for human pathogens since they are generated during the human inflammation response to bacterial infection. However, expression of PT genes in the catalase-deficient
<jats:named-content content-type="genus-species">E. coli</jats:named-content>
Hpx
<jats:sup>−</jats:sup>
strain restores H
<jats:sub>2</jats:sub>
O
<jats:sub>2</jats:sub>
resistance. Here, we seek to solve this obvious paradox. We demonstrate that DndCDE-FeS is a short-lived catalase that binds tightly to PT DNA. It is thus possible that by docking to PT sites the catalase activity protects the bacterial genome against H
<jats:sub>2</jats:sub>
O
<jats:sub>2</jats:sub>
damage.
</jats:p> |
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| author | Pu, Tianning, Liang, Jingdan, Mei, Zhiling, Yang, Yan, Wang, Jialiang, Zhang, Wei, Liang, Wei-Jun, Zhou, Xiufen, Deng, Zixin, Wang, Zhijun |
| author_facet | Pu, Tianning, Liang, Jingdan, Mei, Zhiling, Yang, Yan, Wang, Jialiang, Zhang, Wei, Liang, Wei-Jun, Zhou, Xiufen, Deng, Zixin, Wang, Zhijun, Pu, Tianning, Liang, Jingdan, Mei, Zhiling, Yang, Yan, Wang, Jialiang, Zhang, Wei, Liang, Wei-Jun, Zhou, Xiufen, Deng, Zixin, Wang, Zhijun |
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| description | <jats:p> DNA phosphorothioation has been reported in many bacteria. These PT-hosting bacteria live in very different environments, such as the human body, soil, or hot springs. The physiological function of DNA PT modification is still elusive. A remarkable property of PT modification is that purified genomic PT DNA is susceptible to oxidative cleavage. Among the oxidants, hypochlorous acid and H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> are of physiological relevance for human pathogens since they are generated during the human inflammation response to bacterial infection. However, expression of PT genes in the catalase-deficient <jats:named-content content-type="genus-species">E. coli</jats:named-content> Hpx <jats:sup>−</jats:sup> strain restores H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> resistance. Here, we seek to solve this obvious paradox. We demonstrate that DndCDE-FeS is a short-lived catalase that binds tightly to PT DNA. It is thus possible that by docking to PT sites the catalase activity protects the bacterial genome against H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> damage. </jats:p> |
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| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| language | English |
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| spelling | Pu, Tianning Liang, Jingdan Mei, Zhiling Yang, Yan Wang, Jialiang Zhang, Wei Liang, Wei-Jun Zhou, Xiufen Deng, Zixin Wang, Zhijun 0099-2240 1098-5336 American Society for Microbiology Ecology Applied Microbiology and Biotechnology Food Science Biotechnology http://dx.doi.org/10.1128/aem.00104-19 <jats:p> DNA phosphorothioation has been reported in many bacteria. These PT-hosting bacteria live in very different environments, such as the human body, soil, or hot springs. The physiological function of DNA PT modification is still elusive. A remarkable property of PT modification is that purified genomic PT DNA is susceptible to oxidative cleavage. Among the oxidants, hypochlorous acid and H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> are of physiological relevance for human pathogens since they are generated during the human inflammation response to bacterial infection. However, expression of PT genes in the catalase-deficient <jats:named-content content-type="genus-species">E. coli</jats:named-content> Hpx <jats:sup>−</jats:sup> strain restores H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> resistance. Here, we seek to solve this obvious paradox. We demonstrate that DndCDE-FeS is a short-lived catalase that binds tightly to PT DNA. It is thus possible that by docking to PT sites the catalase activity protects the bacterial genome against H <jats:sub>2</jats:sub> O <jats:sub>2</jats:sub> damage. </jats:p> Phosphorothioated DNA Is Shielded from Oxidative Damage Applied and Environmental Microbiology |
| spellingShingle | Pu, Tianning, Liang, Jingdan, Mei, Zhiling, Yang, Yan, Wang, Jialiang, Zhang, Wei, Liang, Wei-Jun, Zhou, Xiufen, Deng, Zixin, Wang, Zhijun, Applied and Environmental Microbiology, Phosphorothioated DNA Is Shielded from Oxidative Damage, Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| title | Phosphorothioated DNA Is Shielded from Oxidative Damage |
| title_full | Phosphorothioated DNA Is Shielded from Oxidative Damage |
| title_fullStr | Phosphorothioated DNA Is Shielded from Oxidative Damage |
| title_full_unstemmed | Phosphorothioated DNA Is Shielded from Oxidative Damage |
| title_short | Phosphorothioated DNA Is Shielded from Oxidative Damage |
| title_sort | phosphorothioated dna is shielded from oxidative damage |
| title_unstemmed | Phosphorothioated DNA Is Shielded from Oxidative Damage |
| topic | Ecology, Applied Microbiology and Biotechnology, Food Science, Biotechnology |
| url | http://dx.doi.org/10.1128/aem.00104-19 |