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Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1
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| Zeitschriftentitel: | The Journal of Physiology |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | The Journal of Physiology, 589, 2011, 3, S. 495-510 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Bossi, Elena Renna, Maria Daniela Sangaletti, Rachele D’Antoni, Francesca Cherubino, Francesca Kottra, Gabor Peres, Antonio Bossi, Elena Renna, Maria Daniela Sangaletti, Rachele D’Antoni, Francesca Cherubino, Francesca Kottra, Gabor Peres, Antonio |
|---|---|
| author |
Bossi, Elena Renna, Maria Daniela Sangaletti, Rachele D’Antoni, Francesca Cherubino, Francesca Kottra, Gabor Peres, Antonio |
| spellingShingle |
Bossi, Elena Renna, Maria Daniela Sangaletti, Rachele D’Antoni, Francesca Cherubino, Francesca Kottra, Gabor Peres, Antonio The Journal of Physiology Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 Physiology |
| author_sort |
bossi, elena |
| spelling |
Bossi, Elena Renna, Maria Daniela Sangaletti, Rachele D’Antoni, Francesca Cherubino, Francesca Kottra, Gabor Peres, Antonio 0022-3751 1469-7793 Wiley Physiology http://dx.doi.org/10.1113/jphysiol.2010.200469 <jats:title>Non‐technical summary</jats:title><jats:p>The oligopeptide transporter PepT1 is a protein found in the membrane of the cells of the intestinal walls, and represents the main route through which proteic nutrients are absorbed by the organism. Along the polypeptidic chain of this protein, two oppositely charged amino acids, an arginine in position 282 and an aspartate in position 341 of the sequence, have been hypothesised to form a barrier in the absorption pathway. In this paper we show that appropriate mutations of these amino acids change the properties of PepT1 in a way that confirms that these parts of the protein indeed act as an electrostatic gate in the transport process. The identification of the structural basis of the functional mechanism of this transporter is important because, in addition to its role in nutrient uptake, PepT1 represents a major pathway for the absorption of several therapeutic drugs.</jats:p> Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 The Journal of Physiology |
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| title |
Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 |
| title_unstemmed |
Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 |
| title_full |
Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 |
| title_fullStr |
Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 |
| title_full_unstemmed |
Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 |
| title_short |
Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 |
| title_sort |
residues r282 and d341 act as electrostatic gates in the proton‐dependent oligopeptide transporter pept1 |
| topic |
Physiology |
| url |
http://dx.doi.org/10.1113/jphysiol.2010.200469 |
| publishDate |
2011 |
| physical |
495-510 |
| description |
<jats:title>Non‐technical summary</jats:title><jats:p>The oligopeptide transporter PepT1 is a protein found in the membrane of the cells of the intestinal walls, and represents the main route through which proteic nutrients are absorbed by the organism. Along the polypeptidic chain of this protein, two oppositely charged amino acids, an arginine in position 282 and an aspartate in position 341 of the sequence, have been hypothesised to form a barrier in the absorption pathway. In this paper we show that appropriate mutations of these amino acids change the properties of PepT1 in a way that confirms that these parts of the protein indeed act as an electrostatic gate in the transport process. The identification of the structural basis of the functional mechanism of this transporter is important because, in addition to its role in nutrient uptake, PepT1 represents a major pathway for the absorption of several therapeutic drugs.</jats:p> |
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| author | Bossi, Elena, Renna, Maria Daniela, Sangaletti, Rachele, D’Antoni, Francesca, Cherubino, Francesca, Kottra, Gabor, Peres, Antonio |
| author_facet | Bossi, Elena, Renna, Maria Daniela, Sangaletti, Rachele, D’Antoni, Francesca, Cherubino, Francesca, Kottra, Gabor, Peres, Antonio, Bossi, Elena, Renna, Maria Daniela, Sangaletti, Rachele, D’Antoni, Francesca, Cherubino, Francesca, Kottra, Gabor, Peres, Antonio |
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| description | <jats:title>Non‐technical summary</jats:title><jats:p>The oligopeptide transporter PepT1 is a protein found in the membrane of the cells of the intestinal walls, and represents the main route through which proteic nutrients are absorbed by the organism. Along the polypeptidic chain of this protein, two oppositely charged amino acids, an arginine in position 282 and an aspartate in position 341 of the sequence, have been hypothesised to form a barrier in the absorption pathway. In this paper we show that appropriate mutations of these amino acids change the properties of PepT1 in a way that confirms that these parts of the protein indeed act as an electrostatic gate in the transport process. The identification of the structural basis of the functional mechanism of this transporter is important because, in addition to its role in nutrient uptake, PepT1 represents a major pathway for the absorption of several therapeutic drugs.</jats:p> |
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| imprint | Wiley, 2011 |
| imprint_str_mv | Wiley, 2011 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2 |
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| spelling | Bossi, Elena Renna, Maria Daniela Sangaletti, Rachele D’Antoni, Francesca Cherubino, Francesca Kottra, Gabor Peres, Antonio 0022-3751 1469-7793 Wiley Physiology http://dx.doi.org/10.1113/jphysiol.2010.200469 <jats:title>Non‐technical summary</jats:title><jats:p>The oligopeptide transporter PepT1 is a protein found in the membrane of the cells of the intestinal walls, and represents the main route through which proteic nutrients are absorbed by the organism. Along the polypeptidic chain of this protein, two oppositely charged amino acids, an arginine in position 282 and an aspartate in position 341 of the sequence, have been hypothesised to form a barrier in the absorption pathway. In this paper we show that appropriate mutations of these amino acids change the properties of PepT1 in a way that confirms that these parts of the protein indeed act as an electrostatic gate in the transport process. The identification of the structural basis of the functional mechanism of this transporter is important because, in addition to its role in nutrient uptake, PepT1 represents a major pathway for the absorption of several therapeutic drugs.</jats:p> Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 The Journal of Physiology |
| spellingShingle | Bossi, Elena, Renna, Maria Daniela, Sangaletti, Rachele, D’Antoni, Francesca, Cherubino, Francesca, Kottra, Gabor, Peres, Antonio, The Journal of Physiology, Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1, Physiology |
| title | Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 |
| title_full | Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 |
| title_fullStr | Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 |
| title_full_unstemmed | Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 |
| title_short | Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 |
| title_sort | residues r282 and d341 act as electrostatic gates in the proton‐dependent oligopeptide transporter pept1 |
| title_unstemmed | Residues R282 and D341 act as electrostatic gates in the proton‐dependent oligopeptide transporter PepT1 |
| topic | Physiology |
| url | http://dx.doi.org/10.1113/jphysiol.2010.200469 |