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Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties
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| Zeitschriftentitel: | Plant Biotechnology Journal |
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| Personen und Körperschaften: | , , , , , , , , |
| In: | Plant Biotechnology Journal, 12, 2014, 1, S. 17-27 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Pons, Elsa Alquézar, Berta Rodríguez, Ana Martorell, Patricia Genovés, Salvador Ramón, Daniel Rodrigo, María Jesús Zacarías, Lorenzo Peña, Leandro Pons, Elsa Alquézar, Berta Rodríguez, Ana Martorell, Patricia Genovés, Salvador Ramón, Daniel Rodrigo, María Jesús Zacarías, Lorenzo Peña, Leandro |
|---|---|
| author |
Pons, Elsa Alquézar, Berta Rodríguez, Ana Martorell, Patricia Genovés, Salvador Ramón, Daniel Rodrigo, María Jesús Zacarías, Lorenzo Peña, Leandro |
| spellingShingle |
Pons, Elsa Alquézar, Berta Rodríguez, Ana Martorell, Patricia Genovés, Salvador Ramón, Daniel Rodrigo, María Jesús Zacarías, Lorenzo Peña, Leandro Plant Biotechnology Journal Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties Plant Science Agronomy and Crop Science Biotechnology |
| author_sort |
pons, elsa |
| spelling |
Pons, Elsa Alquézar, Berta Rodríguez, Ana Martorell, Patricia Genovés, Salvador Ramón, Daniel Rodrigo, María Jesús Zacarías, Lorenzo Peña, Leandro 1467-7644 1467-7652 Wiley Plant Science Agronomy and Crop Science Biotechnology http://dx.doi.org/10.1111/pbi.12112 <jats:title>Summary</jats:title><jats:p>Orange is a major crop and an important source of health‐promoting bioactive compounds. Increasing the levels of specific antioxidants in orange fruit through metabolic engineering could strengthen the fruit's health benefits. In this work, we have afforded enhancing the β‐carotene content of orange fruit through blocking by <jats:styled-content style="fixed-case">RNA</jats:styled-content> interference the expression of an endogenous β‐carotene hydroxylase gene (<jats:styled-content style="fixed-case"><jats:italic>Csβ‐CHX</jats:italic></jats:styled-content>) that is involved in the conversion of β‐carotene into xanthophylls. Additionally, we have simultaneously overexpressed a key regulator gene of flowering transition, the <jats:styled-content style="fixed-case"><jats:italic>FLOWERING LOCUS T</jats:italic></jats:styled-content> from sweet orange (<jats:styled-content style="fixed-case"><jats:italic>CsFT</jats:italic></jats:styled-content>), in the transgenic juvenile plants, which allowed us to obtain fruit in an extremely short period of time. Silencing the <jats:styled-content style="fixed-case"><jats:italic>Csβ‐CHX</jats:italic></jats:styled-content> gene resulted in oranges with a deep yellow (‘golden’) phenotype and significant increases (up to 36‐fold) in β‐carotene content in the pulp. The capacity of β‐carotene‐enriched oranges for protection against oxidative stress <jats:italic>in vivo</jats:italic> was assessed using <jats:italic><jats:styled-content style="fixed-case">C</jats:styled-content>aenorhabditis elegans</jats:italic> as experimental animal model. Golden oranges induced a 20% higher antioxidant effect than the isogenic control. This is the first example of the successful metabolic engineering of the β‐carotene content (or the content of any other phytonutrient) in oranges and demonstrates the potential of genetic engineering for the nutritional enhancement of fruit tree crops.</jats:p> Metabolic engineering of β‐carotene in orange fruit increases its <i>in vivo</i> antioxidant properties Plant Biotechnology Journal |
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10.1111/pbi.12112 |
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Biologie Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft Technik |
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| title |
Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties |
| title_unstemmed |
Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties |
| title_full |
Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties |
| title_fullStr |
Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties |
| title_full_unstemmed |
Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties |
| title_short |
Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties |
| title_sort |
metabolic engineering of β‐carotene in orange fruit increases its <i>in vivo</i> antioxidant properties |
| topic |
Plant Science Agronomy and Crop Science Biotechnology |
| url |
http://dx.doi.org/10.1111/pbi.12112 |
| publishDate |
2014 |
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17-27 |
| description |
<jats:title>Summary</jats:title><jats:p>Orange is a major crop and an important source of health‐promoting bioactive compounds. Increasing the levels of specific antioxidants in orange fruit through metabolic engineering could strengthen the fruit's health benefits. In this work, we have afforded enhancing the β‐carotene content of orange fruit through blocking by <jats:styled-content style="fixed-case">RNA</jats:styled-content> interference the expression of an endogenous β‐carotene hydroxylase gene (<jats:styled-content style="fixed-case"><jats:italic>Csβ‐CHX</jats:italic></jats:styled-content>) that is involved in the conversion of β‐carotene into xanthophylls. Additionally, we have simultaneously overexpressed a key regulator gene of flowering transition, the <jats:styled-content style="fixed-case"><jats:italic>FLOWERING LOCUS T</jats:italic></jats:styled-content> from sweet orange (<jats:styled-content style="fixed-case"><jats:italic>CsFT</jats:italic></jats:styled-content>), in the transgenic juvenile plants, which allowed us to obtain fruit in an extremely short period of time. Silencing the <jats:styled-content style="fixed-case"><jats:italic>Csβ‐CHX</jats:italic></jats:styled-content> gene resulted in oranges with a deep yellow (‘golden’) phenotype and significant increases (up to 36‐fold) in β‐carotene content in the pulp. The capacity of β‐carotene‐enriched oranges for protection against oxidative stress <jats:italic>in vivo</jats:italic> was assessed using <jats:italic><jats:styled-content style="fixed-case">C</jats:styled-content>aenorhabditis elegans</jats:italic> as experimental animal model. Golden oranges induced a 20% higher antioxidant effect than the isogenic control. This is the first example of the successful metabolic engineering of the β‐carotene content (or the content of any other phytonutrient) in oranges and demonstrates the potential of genetic engineering for the nutritional enhancement of fruit tree crops.</jats:p> |
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| author | Pons, Elsa, Alquézar, Berta, Rodríguez, Ana, Martorell, Patricia, Genovés, Salvador, Ramón, Daniel, Rodrigo, María Jesús, Zacarías, Lorenzo, Peña, Leandro |
| author_facet | Pons, Elsa, Alquézar, Berta, Rodríguez, Ana, Martorell, Patricia, Genovés, Salvador, Ramón, Daniel, Rodrigo, María Jesús, Zacarías, Lorenzo, Peña, Leandro, Pons, Elsa, Alquézar, Berta, Rodríguez, Ana, Martorell, Patricia, Genovés, Salvador, Ramón, Daniel, Rodrigo, María Jesús, Zacarías, Lorenzo, Peña, Leandro |
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| description | <jats:title>Summary</jats:title><jats:p>Orange is a major crop and an important source of health‐promoting bioactive compounds. Increasing the levels of specific antioxidants in orange fruit through metabolic engineering could strengthen the fruit's health benefits. In this work, we have afforded enhancing the β‐carotene content of orange fruit through blocking by <jats:styled-content style="fixed-case">RNA</jats:styled-content> interference the expression of an endogenous β‐carotene hydroxylase gene (<jats:styled-content style="fixed-case"><jats:italic>Csβ‐CHX</jats:italic></jats:styled-content>) that is involved in the conversion of β‐carotene into xanthophylls. Additionally, we have simultaneously overexpressed a key regulator gene of flowering transition, the <jats:styled-content style="fixed-case"><jats:italic>FLOWERING LOCUS T</jats:italic></jats:styled-content> from sweet orange (<jats:styled-content style="fixed-case"><jats:italic>CsFT</jats:italic></jats:styled-content>), in the transgenic juvenile plants, which allowed us to obtain fruit in an extremely short period of time. Silencing the <jats:styled-content style="fixed-case"><jats:italic>Csβ‐CHX</jats:italic></jats:styled-content> gene resulted in oranges with a deep yellow (‘golden’) phenotype and significant increases (up to 36‐fold) in β‐carotene content in the pulp. The capacity of β‐carotene‐enriched oranges for protection against oxidative stress <jats:italic>in vivo</jats:italic> was assessed using <jats:italic><jats:styled-content style="fixed-case">C</jats:styled-content>aenorhabditis elegans</jats:italic> as experimental animal model. Golden oranges induced a 20% higher antioxidant effect than the isogenic control. This is the first example of the successful metabolic engineering of the β‐carotene content (or the content of any other phytonutrient) in oranges and demonstrates the potential of genetic engineering for the nutritional enhancement of fruit tree crops.</jats:p> |
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| spelling | Pons, Elsa Alquézar, Berta Rodríguez, Ana Martorell, Patricia Genovés, Salvador Ramón, Daniel Rodrigo, María Jesús Zacarías, Lorenzo Peña, Leandro 1467-7644 1467-7652 Wiley Plant Science Agronomy and Crop Science Biotechnology http://dx.doi.org/10.1111/pbi.12112 <jats:title>Summary</jats:title><jats:p>Orange is a major crop and an important source of health‐promoting bioactive compounds. Increasing the levels of specific antioxidants in orange fruit through metabolic engineering could strengthen the fruit's health benefits. In this work, we have afforded enhancing the β‐carotene content of orange fruit through blocking by <jats:styled-content style="fixed-case">RNA</jats:styled-content> interference the expression of an endogenous β‐carotene hydroxylase gene (<jats:styled-content style="fixed-case"><jats:italic>Csβ‐CHX</jats:italic></jats:styled-content>) that is involved in the conversion of β‐carotene into xanthophylls. Additionally, we have simultaneously overexpressed a key regulator gene of flowering transition, the <jats:styled-content style="fixed-case"><jats:italic>FLOWERING LOCUS T</jats:italic></jats:styled-content> from sweet orange (<jats:styled-content style="fixed-case"><jats:italic>CsFT</jats:italic></jats:styled-content>), in the transgenic juvenile plants, which allowed us to obtain fruit in an extremely short period of time. Silencing the <jats:styled-content style="fixed-case"><jats:italic>Csβ‐CHX</jats:italic></jats:styled-content> gene resulted in oranges with a deep yellow (‘golden’) phenotype and significant increases (up to 36‐fold) in β‐carotene content in the pulp. The capacity of β‐carotene‐enriched oranges for protection against oxidative stress <jats:italic>in vivo</jats:italic> was assessed using <jats:italic><jats:styled-content style="fixed-case">C</jats:styled-content>aenorhabditis elegans</jats:italic> as experimental animal model. Golden oranges induced a 20% higher antioxidant effect than the isogenic control. This is the first example of the successful metabolic engineering of the β‐carotene content (or the content of any other phytonutrient) in oranges and demonstrates the potential of genetic engineering for the nutritional enhancement of fruit tree crops.</jats:p> Metabolic engineering of β‐carotene in orange fruit increases its <i>in vivo</i> antioxidant properties Plant Biotechnology Journal |
| spellingShingle | Pons, Elsa, Alquézar, Berta, Rodríguez, Ana, Martorell, Patricia, Genovés, Salvador, Ramón, Daniel, Rodrigo, María Jesús, Zacarías, Lorenzo, Peña, Leandro, Plant Biotechnology Journal, Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties, Plant Science, Agronomy and Crop Science, Biotechnology |
| title | Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties |
| title_full | Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties |
| title_fullStr | Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties |
| title_full_unstemmed | Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties |
| title_short | Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties |
| title_sort | metabolic engineering of β‐carotene in orange fruit increases its <i>in vivo</i> antioxidant properties |
| title_unstemmed | Metabolic engineering of β‐carotene in orange fruit increases its in vivo antioxidant properties |
| topic | Plant Science, Agronomy and Crop Science, Biotechnology |
| url | http://dx.doi.org/10.1111/pbi.12112 |