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Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress
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| Zeitschriftentitel: | Molecular Ecology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Molecular Ecology, 26, 2017, 11, S. 2978-2992 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Bentley, Blair P. Haas, Brian J. Tedeschi, Jamie N. Berry, Oliver Bentley, Blair P. Haas, Brian J. Tedeschi, Jamie N. Berry, Oliver |
|---|---|
| author |
Bentley, Blair P. Haas, Brian J. Tedeschi, Jamie N. Berry, Oliver |
| spellingShingle |
Bentley, Blair P. Haas, Brian J. Tedeschi, Jamie N. Berry, Oliver Molecular Ecology Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress Genetics Ecology, Evolution, Behavior and Systematics |
| author_sort |
bentley, blair p. |
| spelling |
Bentley, Blair P. Haas, Brian J. Tedeschi, Jamie N. Berry, Oliver 0962-1083 1365-294X Wiley Genetics Ecology, Evolution, Behavior and Systematics http://dx.doi.org/10.1111/mec.14087 <jats:title>Abstract</jats:title><jats:p>Oviparous reptile embryos are expected to breach their critical thermal maxima if temperatures reach those predicted under current climate change models due to the lack of the maternal buffering processes and parental care. Heat‐shock proteins (HSPs) are integral in the molecular response to thermal stress, and their expression is heritable, but the roles of other candidate families such as the heat‐shock factors (<jats:styled-content style="fixed-case">HSF</jats:styled-content>s) have not been determined in reptiles. Here, we subject embryonic sea turtles (<jats:italic>Caretta caretta</jats:italic>) to a biologically realistic thermal stress and employ de novo transcriptomic profiling of brain tissue to investigate the underlying molecular response. From a reference transcriptome of 302 293 transcripts, 179 were identified as differentially expressed between treatments. As anticipated, genes enriched in the heat‐shock treatment were primarily associated with the Hsp families, or were genes whose products play similar protein editing and chaperone functions (e.g. <jats:italic>bag3</jats:italic>,<jats:italic> <jats:styled-content style="fixed-case">MYOC</jats:styled-content></jats:italic> and <jats:italic>serpinh1</jats:italic>). Unexpectedly, genes encoding the <jats:styled-content style="fixed-case">HSF</jats:styled-content>s were not significantly upregulated under thermal stress, indicating their presence in unstressed cells in an inactive state. Genes that were downregulated under thermal stress were less well functionally defined but were associated with stress response, development and cellular organization, suggesting that developmental processes may be compromised at realistically high temperatures. These results confirm that genes from the Hsp families play vital roles in the thermal tolerance of developing reptile embryos and, in addition with a number of other genes, should be targets for evaluating the capacity of oviparous reptiles to respond adaptively to the effects of climate change.</jats:p> Loggerhead sea turtle embryos (<i>Caretta caretta</i>) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress Molecular Ecology |
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Biologie Geographie |
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Wiley, 2017 |
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Molecular Ecology |
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| title |
Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| title_unstemmed |
Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| title_full |
Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| title_fullStr |
Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| title_full_unstemmed |
Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| title_short |
Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| title_sort |
loggerhead sea turtle embryos (<i>caretta caretta</i>) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| topic |
Genetics Ecology, Evolution, Behavior and Systematics |
| url |
http://dx.doi.org/10.1111/mec.14087 |
| publishDate |
2017 |
| physical |
2978-2992 |
| description |
<jats:title>Abstract</jats:title><jats:p>Oviparous reptile embryos are expected to breach their critical thermal maxima if temperatures reach those predicted under current climate change models due to the lack of the maternal buffering processes and parental care. Heat‐shock proteins (HSPs) are integral in the molecular response to thermal stress, and their expression is heritable, but the roles of other candidate families such as the heat‐shock factors (<jats:styled-content style="fixed-case">HSF</jats:styled-content>s) have not been determined in reptiles. Here, we subject embryonic sea turtles (<jats:italic>Caretta caretta</jats:italic>) to a biologically realistic thermal stress and employ de novo transcriptomic profiling of brain tissue to investigate the underlying molecular response. From a reference transcriptome of 302 293 transcripts, 179 were identified as differentially expressed between treatments. As anticipated, genes enriched in the heat‐shock treatment were primarily associated with the Hsp families, or were genes whose products play similar protein editing and chaperone functions (e.g. <jats:italic>bag3</jats:italic>,<jats:italic> <jats:styled-content style="fixed-case">MYOC</jats:styled-content></jats:italic> and <jats:italic>serpinh1</jats:italic>). Unexpectedly, genes encoding the <jats:styled-content style="fixed-case">HSF</jats:styled-content>s were not significantly upregulated under thermal stress, indicating their presence in unstressed cells in an inactive state. Genes that were downregulated under thermal stress were less well functionally defined but were associated with stress response, development and cellular organization, suggesting that developmental processes may be compromised at realistically high temperatures. These results confirm that genes from the Hsp families play vital roles in the thermal tolerance of developing reptile embryos and, in addition with a number of other genes, should be targets for evaluating the capacity of oviparous reptiles to respond adaptively to the effects of climate change.</jats:p> |
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| author | Bentley, Blair P., Haas, Brian J., Tedeschi, Jamie N., Berry, Oliver |
| author_facet | Bentley, Blair P., Haas, Brian J., Tedeschi, Jamie N., Berry, Oliver, Bentley, Blair P., Haas, Brian J., Tedeschi, Jamie N., Berry, Oliver |
| author_sort | bentley, blair p. |
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| description | <jats:title>Abstract</jats:title><jats:p>Oviparous reptile embryos are expected to breach their critical thermal maxima if temperatures reach those predicted under current climate change models due to the lack of the maternal buffering processes and parental care. Heat‐shock proteins (HSPs) are integral in the molecular response to thermal stress, and their expression is heritable, but the roles of other candidate families such as the heat‐shock factors (<jats:styled-content style="fixed-case">HSF</jats:styled-content>s) have not been determined in reptiles. Here, we subject embryonic sea turtles (<jats:italic>Caretta caretta</jats:italic>) to a biologically realistic thermal stress and employ de novo transcriptomic profiling of brain tissue to investigate the underlying molecular response. From a reference transcriptome of 302 293 transcripts, 179 were identified as differentially expressed between treatments. As anticipated, genes enriched in the heat‐shock treatment were primarily associated with the Hsp families, or were genes whose products play similar protein editing and chaperone functions (e.g. <jats:italic>bag3</jats:italic>,<jats:italic> <jats:styled-content style="fixed-case">MYOC</jats:styled-content></jats:italic> and <jats:italic>serpinh1</jats:italic>). Unexpectedly, genes encoding the <jats:styled-content style="fixed-case">HSF</jats:styled-content>s were not significantly upregulated under thermal stress, indicating their presence in unstressed cells in an inactive state. Genes that were downregulated under thermal stress were less well functionally defined but were associated with stress response, development and cellular organization, suggesting that developmental processes may be compromised at realistically high temperatures. These results confirm that genes from the Hsp families play vital roles in the thermal tolerance of developing reptile embryos and, in addition with a number of other genes, should be targets for evaluating the capacity of oviparous reptiles to respond adaptively to the effects of climate change.</jats:p> |
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| imprint | Wiley, 2017 |
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| institution | DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15 |
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| language | English |
| last_indexed | 2024-03-01T15:51:13.787Z |
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| series | Molecular Ecology |
| source_id | 49 |
| spelling | Bentley, Blair P. Haas, Brian J. Tedeschi, Jamie N. Berry, Oliver 0962-1083 1365-294X Wiley Genetics Ecology, Evolution, Behavior and Systematics http://dx.doi.org/10.1111/mec.14087 <jats:title>Abstract</jats:title><jats:p>Oviparous reptile embryos are expected to breach their critical thermal maxima if temperatures reach those predicted under current climate change models due to the lack of the maternal buffering processes and parental care. Heat‐shock proteins (HSPs) are integral in the molecular response to thermal stress, and their expression is heritable, but the roles of other candidate families such as the heat‐shock factors (<jats:styled-content style="fixed-case">HSF</jats:styled-content>s) have not been determined in reptiles. Here, we subject embryonic sea turtles (<jats:italic>Caretta caretta</jats:italic>) to a biologically realistic thermal stress and employ de novo transcriptomic profiling of brain tissue to investigate the underlying molecular response. From a reference transcriptome of 302 293 transcripts, 179 were identified as differentially expressed between treatments. As anticipated, genes enriched in the heat‐shock treatment were primarily associated with the Hsp families, or were genes whose products play similar protein editing and chaperone functions (e.g. <jats:italic>bag3</jats:italic>,<jats:italic> <jats:styled-content style="fixed-case">MYOC</jats:styled-content></jats:italic> and <jats:italic>serpinh1</jats:italic>). Unexpectedly, genes encoding the <jats:styled-content style="fixed-case">HSF</jats:styled-content>s were not significantly upregulated under thermal stress, indicating their presence in unstressed cells in an inactive state. Genes that were downregulated under thermal stress were less well functionally defined but were associated with stress response, development and cellular organization, suggesting that developmental processes may be compromised at realistically high temperatures. These results confirm that genes from the Hsp families play vital roles in the thermal tolerance of developing reptile embryos and, in addition with a number of other genes, should be targets for evaluating the capacity of oviparous reptiles to respond adaptively to the effects of climate change.</jats:p> Loggerhead sea turtle embryos (<i>Caretta caretta</i>) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress Molecular Ecology |
| spellingShingle | Bentley, Blair P., Haas, Brian J., Tedeschi, Jamie N., Berry, Oliver, Molecular Ecology, Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress, Genetics, Ecology, Evolution, Behavior and Systematics |
| title | Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| title_full | Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| title_fullStr | Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| title_full_unstemmed | Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| title_short | Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| title_sort | loggerhead sea turtle embryos (<i>caretta caretta</i>) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| title_unstemmed | Loggerhead sea turtle embryos (Caretta caretta) regulate expression of stress response and developmental genes when exposed to a biologically realistic heat stress |
| topic | Genetics, Ecology, Evolution, Behavior and Systematics |
| url | http://dx.doi.org/10.1111/mec.14087 |