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microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury
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| Zeitschriftentitel: | Journal of Neurochemistry |
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| Personen und Körperschaften: | , , , , , , , , , |
| In: | Journal of Neurochemistry, 153, 2020, 6, S. 710-726 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Wu, Jingchuan He, Junchi Tian, Xiaocui Luo, Yuetao Zhong, Jianjun Zhang, Hongrong Li, Hui Cen, Bo Jiang, Tao Sun, Xiaochuan Wu, Jingchuan He, Junchi Tian, Xiaocui Luo, Yuetao Zhong, Jianjun Zhang, Hongrong Li, Hui Cen, Bo Jiang, Tao Sun, Xiaochuan |
|---|---|
| author |
Wu, Jingchuan He, Junchi Tian, Xiaocui Luo, Yuetao Zhong, Jianjun Zhang, Hongrong Li, Hui Cen, Bo Jiang, Tao Sun, Xiaochuan |
| spellingShingle |
Wu, Jingchuan He, Junchi Tian, Xiaocui Luo, Yuetao Zhong, Jianjun Zhang, Hongrong Li, Hui Cen, Bo Jiang, Tao Sun, Xiaochuan Journal of Neurochemistry microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury Cellular and Molecular Neuroscience Biochemistry |
| author_sort |
wu, jingchuan |
| spelling |
Wu, Jingchuan He, Junchi Tian, Xiaocui Luo, Yuetao Zhong, Jianjun Zhang, Hongrong Li, Hui Cen, Bo Jiang, Tao Sun, Xiaochuan 0022-3042 1471-4159 Wiley Cellular and Molecular Neuroscience Biochemistry http://dx.doi.org/10.1111/jnc.14963 <jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>The level of microRNA‐9‐5p (miRNA‐9‐5p) in brain tissues is significantly changed after traumatic brain injury (TBI). However, the effect of miRNA‐9‐5p for brain function in TBI has not been elucidated. In this study, a controlled cortical impact model was used to induce TBI in Sprague–Dawley rats, and an oxygen glucose deprivation model was used to mimic the pathological state in vitro. Brain microvascular endothelial cells (BMECs) and astrocytes were extracted from immature Sprague–Dawley rats and cocultured to reconstruct blood–brain barrier (BBB) in vitro. The results show that the level of miRNA‐9‐5p was significantly increased in brain tissues after TBI, and up‐regulation of miRNA9‐5p contributed to the recovery of neurological function. Up‐regulation of miRNA‐9‐5p with miRNA agomir may significantly alleviate apoptosis, neuroinflammation, and BBB damage in rats after TBI. Moreover, a dual luciferase reporter assay confirmed that miRNA‐9‐5p is a post‐transcriptional modulator of Ptch‐1. In in vitro experiments, the results confirmed that up‐regulation of miRNA‐9‐5p with miRNA mimic alleviates cellular apoptosis, inflammatory response, and BBB damage mainly by inhibiting Ptch‐1. In addition, we found that the activation of Hedgehog pathway was accompanied by inhibition of NF‐κB/MMP‐9 pathway in the BMECs treated with miRNA‐9‐5p mimic. Taken together, these results indicate that up‐regulation of miRNA‐9‐5p alleviates BBB damage and neuroinflammatory responses by activating the Hedgehog pathway and inhibiting NF‐κB/MMP‐9 pathway, which promotes the recovery of neurological function after TBI.</jats:p></jats:sec><jats:sec><jats:label /><jats:p> <jats:boxed-text content-type="graphic" position="anchor"><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" mimetype="image/png" position="anchor" specific-use="enlarged-web-image" xlink:href="graphic/jnc14963-fig-0010-m.png"><jats:alt-text>image</jats:alt-text></jats:graphic></jats:boxed-text> </jats:p></jats:sec> microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury Journal of Neurochemistry |
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10.1111/jnc.14963 |
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Biologie Psychologie Chemie und Pharmazie |
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Wiley, 2020 |
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| title |
microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| title_unstemmed |
microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| title_full |
microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| title_fullStr |
microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| title_full_unstemmed |
microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| title_short |
microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| title_sort |
microrna‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| topic |
Cellular and Molecular Neuroscience Biochemistry |
| url |
http://dx.doi.org/10.1111/jnc.14963 |
| publishDate |
2020 |
| physical |
710-726 |
| description |
<jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>The level of microRNA‐9‐5p (miRNA‐9‐5p) in brain tissues is significantly changed after traumatic brain injury (TBI). However, the effect of miRNA‐9‐5p for brain function in TBI has not been elucidated. In this study, a controlled cortical impact model was used to induce TBI in Sprague–Dawley rats, and an oxygen glucose deprivation model was used to mimic the pathological state in vitro. Brain microvascular endothelial cells (BMECs) and astrocytes were extracted from immature Sprague–Dawley rats and cocultured to reconstruct blood–brain barrier (BBB) in vitro. The results show that the level of miRNA‐9‐5p was significantly increased in brain tissues after TBI, and up‐regulation of miRNA9‐5p contributed to the recovery of neurological function. Up‐regulation of miRNA‐9‐5p with miRNA agomir may significantly alleviate apoptosis, neuroinflammation, and BBB damage in rats after TBI. Moreover, a dual luciferase reporter assay confirmed that miRNA‐9‐5p is a post‐transcriptional modulator of Ptch‐1. In in vitro experiments, the results confirmed that up‐regulation of miRNA‐9‐5p with miRNA mimic alleviates cellular apoptosis, inflammatory response, and BBB damage mainly by inhibiting Ptch‐1. In addition, we found that the activation of Hedgehog pathway was accompanied by inhibition of NF‐κB/MMP‐9 pathway in the BMECs treated with miRNA‐9‐5p mimic. Taken together, these results indicate that up‐regulation of miRNA‐9‐5p alleviates BBB damage and neuroinflammatory responses by activating the Hedgehog pathway and inhibiting NF‐κB/MMP‐9 pathway, which promotes the recovery of neurological function after TBI.</jats:p></jats:sec><jats:sec><jats:label /><jats:p>
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| author | Wu, Jingchuan, He, Junchi, Tian, Xiaocui, Luo, Yuetao, Zhong, Jianjun, Zhang, Hongrong, Li, Hui, Cen, Bo, Jiang, Tao, Sun, Xiaochuan |
| author_facet | Wu, Jingchuan, He, Junchi, Tian, Xiaocui, Luo, Yuetao, Zhong, Jianjun, Zhang, Hongrong, Li, Hui, Cen, Bo, Jiang, Tao, Sun, Xiaochuan, Wu, Jingchuan, He, Junchi, Tian, Xiaocui, Luo, Yuetao, Zhong, Jianjun, Zhang, Hongrong, Li, Hui, Cen, Bo, Jiang, Tao, Sun, Xiaochuan |
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| description | <jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>The level of microRNA‐9‐5p (miRNA‐9‐5p) in brain tissues is significantly changed after traumatic brain injury (TBI). However, the effect of miRNA‐9‐5p for brain function in TBI has not been elucidated. In this study, a controlled cortical impact model was used to induce TBI in Sprague–Dawley rats, and an oxygen glucose deprivation model was used to mimic the pathological state in vitro. Brain microvascular endothelial cells (BMECs) and astrocytes were extracted from immature Sprague–Dawley rats and cocultured to reconstruct blood–brain barrier (BBB) in vitro. The results show that the level of miRNA‐9‐5p was significantly increased in brain tissues after TBI, and up‐regulation of miRNA9‐5p contributed to the recovery of neurological function. Up‐regulation of miRNA‐9‐5p with miRNA agomir may significantly alleviate apoptosis, neuroinflammation, and BBB damage in rats after TBI. Moreover, a dual luciferase reporter assay confirmed that miRNA‐9‐5p is a post‐transcriptional modulator of Ptch‐1. In in vitro experiments, the results confirmed that up‐regulation of miRNA‐9‐5p with miRNA mimic alleviates cellular apoptosis, inflammatory response, and BBB damage mainly by inhibiting Ptch‐1. In addition, we found that the activation of Hedgehog pathway was accompanied by inhibition of NF‐κB/MMP‐9 pathway in the BMECs treated with miRNA‐9‐5p mimic. Taken together, these results indicate that up‐regulation of miRNA‐9‐5p alleviates BBB damage and neuroinflammatory responses by activating the Hedgehog pathway and inhibiting NF‐κB/MMP‐9 pathway, which promotes the recovery of neurological function after TBI.</jats:p></jats:sec><jats:sec><jats:label /><jats:p> <jats:boxed-text content-type="graphic" position="anchor"><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" mimetype="image/png" position="anchor" specific-use="enlarged-web-image" xlink:href="graphic/jnc14963-fig-0010-m.png"><jats:alt-text>image</jats:alt-text></jats:graphic></jats:boxed-text> </jats:p></jats:sec> |
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| spelling | Wu, Jingchuan He, Junchi Tian, Xiaocui Luo, Yuetao Zhong, Jianjun Zhang, Hongrong Li, Hui Cen, Bo Jiang, Tao Sun, Xiaochuan 0022-3042 1471-4159 Wiley Cellular and Molecular Neuroscience Biochemistry http://dx.doi.org/10.1111/jnc.14963 <jats:title>Abstract</jats:title><jats:sec><jats:label /><jats:p>The level of microRNA‐9‐5p (miRNA‐9‐5p) in brain tissues is significantly changed after traumatic brain injury (TBI). However, the effect of miRNA‐9‐5p for brain function in TBI has not been elucidated. In this study, a controlled cortical impact model was used to induce TBI in Sprague–Dawley rats, and an oxygen glucose deprivation model was used to mimic the pathological state in vitro. Brain microvascular endothelial cells (BMECs) and astrocytes were extracted from immature Sprague–Dawley rats and cocultured to reconstruct blood–brain barrier (BBB) in vitro. The results show that the level of miRNA‐9‐5p was significantly increased in brain tissues after TBI, and up‐regulation of miRNA9‐5p contributed to the recovery of neurological function. Up‐regulation of miRNA‐9‐5p with miRNA agomir may significantly alleviate apoptosis, neuroinflammation, and BBB damage in rats after TBI. Moreover, a dual luciferase reporter assay confirmed that miRNA‐9‐5p is a post‐transcriptional modulator of Ptch‐1. In in vitro experiments, the results confirmed that up‐regulation of miRNA‐9‐5p with miRNA mimic alleviates cellular apoptosis, inflammatory response, and BBB damage mainly by inhibiting Ptch‐1. In addition, we found that the activation of Hedgehog pathway was accompanied by inhibition of NF‐κB/MMP‐9 pathway in the BMECs treated with miRNA‐9‐5p mimic. Taken together, these results indicate that up‐regulation of miRNA‐9‐5p alleviates BBB damage and neuroinflammatory responses by activating the Hedgehog pathway and inhibiting NF‐κB/MMP‐9 pathway, which promotes the recovery of neurological function after TBI.</jats:p></jats:sec><jats:sec><jats:label /><jats:p> <jats:boxed-text content-type="graphic" position="anchor"><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" mimetype="image/png" position="anchor" specific-use="enlarged-web-image" xlink:href="graphic/jnc14963-fig-0010-m.png"><jats:alt-text>image</jats:alt-text></jats:graphic></jats:boxed-text> </jats:p></jats:sec> microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury Journal of Neurochemistry |
| spellingShingle | Wu, Jingchuan, He, Junchi, Tian, Xiaocui, Luo, Yuetao, Zhong, Jianjun, Zhang, Hongrong, Li, Hui, Cen, Bo, Jiang, Tao, Sun, Xiaochuan, Journal of Neurochemistry, microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury, Cellular and Molecular Neuroscience, Biochemistry |
| title | microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| title_full | microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| title_fullStr | microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| title_full_unstemmed | microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| title_short | microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| title_sort | microrna‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| title_unstemmed | microRNA‐9‐5p alleviates blood–brain barrier damage and neuroinflammation after traumatic brain injury |
| topic | Cellular and Molecular Neuroscience, Biochemistry |
| url | http://dx.doi.org/10.1111/jnc.14963 |