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Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity
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| Zeitschriftentitel: | Journal of Cellular and Molecular Medicine |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , , , , , , |
| In: | Journal of Cellular and Molecular Medicine, 23, 2019, 2, S. 1448-1457 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Guan, Yi Gao, Xueting Tang, Qiuyu Huang, Lin Gao, Siyun Yu, Shuai Huang, Jiale Li, Jun Zhou, Daizhan Zhang, Yangyang Shi, Dan Liang, Dandan Liu, Yi Li, Li Cui, Yingyu Xu, Liang Chen, Yi‐Han Guan, Yi Gao, Xueting Tang, Qiuyu Huang, Lin Gao, Siyun Yu, Shuai Huang, Jiale Li, Jun Zhou, Daizhan Zhang, Yangyang Shi, Dan Liang, Dandan Liu, Yi Li, Li Cui, Yingyu Xu, Liang Chen, Yi‐Han |
|---|---|
| author |
Guan, Yi Gao, Xueting Tang, Qiuyu Huang, Lin Gao, Siyun Yu, Shuai Huang, Jiale Li, Jun Zhou, Daizhan Zhang, Yangyang Shi, Dan Liang, Dandan Liu, Yi Li, Li Cui, Yingyu Xu, Liang Chen, Yi‐Han |
| spellingShingle |
Guan, Yi Gao, Xueting Tang, Qiuyu Huang, Lin Gao, Siyun Yu, Shuai Huang, Jiale Li, Jun Zhou, Daizhan Zhang, Yangyang Shi, Dan Liang, Dandan Liu, Yi Li, Li Cui, Yingyu Xu, Liang Chen, Yi‐Han Journal of Cellular and Molecular Medicine Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity Cell Biology Molecular Medicine |
| author_sort |
guan, yi |
| spelling |
Guan, Yi Gao, Xueting Tang, Qiuyu Huang, Lin Gao, Siyun Yu, Shuai Huang, Jiale Li, Jun Zhou, Daizhan Zhang, Yangyang Shi, Dan Liang, Dandan Liu, Yi Li, Li Cui, Yingyu Xu, Liang Chen, Yi‐Han 1582-1838 1582-4934 Wiley Cell Biology Molecular Medicine http://dx.doi.org/10.1111/jcmm.14051 <jats:title>Abstract</jats:title><jats:p>Nucleoporins (Nups) are known to be functional in nucleo‐cytoplasmic transport, but the roles of nucleoporins in nonproliferating cells, such as cardiac myocytes, are still poorly understood. In this study, we report that Nup107 regulates cardiac bioelectricity by controlling the nucleo‐cytoplasmic trafficking of <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content>. Overexpression of Nup107 induced the protein expression of <jats:italic>Scn5a</jats:italic> rather than that of other ion channels, with no effects of their <jats:styled-content style="fixed-case">mRNA</jats:styled-content> levels. The analysis for the protein production demonstrated Nup107‐facilitated transport of <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content>. Using <jats:styled-content style="fixed-case">RIP</jats:styled-content>‐<jats:styled-content style="fixed-case">PCR</jats:styled-content> and luciferase assay, we found that the 5′‐<jats:styled-content style="fixed-case">UTR</jats:styled-content> of <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content> was not involved in the interaction, whereas the spatial interaction between Nup107 protein and <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content> was formed when <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content> passing through the nuclear pore. Functionally, Nup107 overexpression in neonatal rat ventricle myocytes significantly increased the currents of <jats:italic>Scn5a</jats:italic>‐encoded I<jats:sub>Na</jats:sub> channel. Moreover, the close correlation between Nup107 and Nav1.5 protein expression was observed in cardiomycytes and heart tissues subjected to hypoxia and ischaemic insults, suggesting a fast regulation of Nup107 on Nav1.5 channel in cardiac myocytes in a posttranscriptional manner. These findings may provide insights into the emergent control of cardiac electrophysiology through Nup‐mediated modulation of ion channels.</jats:p> Nucleoporin 107 facilitates the nuclear export of <i>Scn5a </i><scp>mRNA</scp> to regulate cardiac bioelectricity Journal of Cellular and Molecular Medicine |
| doi_str_mv |
10.1111/jcmm.14051 |
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Online Free |
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Biologie Medizin |
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ElectronicArticle |
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Wiley, 2019 |
| imprint_str_mv |
Wiley, 2019 |
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Journal of Cellular and Molecular Medicine |
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| title |
Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity |
| title_unstemmed |
Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity |
| title_full |
Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity |
| title_fullStr |
Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity |
| title_full_unstemmed |
Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity |
| title_short |
Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity |
| title_sort |
nucleoporin 107 facilitates the nuclear export of <i>scn5a </i><scp>mrna</scp> to regulate cardiac bioelectricity |
| topic |
Cell Biology Molecular Medicine |
| url |
http://dx.doi.org/10.1111/jcmm.14051 |
| publishDate |
2019 |
| physical |
1448-1457 |
| description |
<jats:title>Abstract</jats:title><jats:p>Nucleoporins (Nups) are known to be functional in nucleo‐cytoplasmic transport, but the roles of nucleoporins in nonproliferating cells, such as cardiac myocytes, are still poorly understood. In this study, we report that Nup107 regulates cardiac bioelectricity by controlling the nucleo‐cytoplasmic trafficking of <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content>. Overexpression of Nup107 induced the protein expression of <jats:italic>Scn5a</jats:italic> rather than that of other ion channels, with no effects of their <jats:styled-content style="fixed-case">mRNA</jats:styled-content> levels. The analysis for the protein production demonstrated Nup107‐facilitated transport of <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content>. Using <jats:styled-content style="fixed-case">RIP</jats:styled-content>‐<jats:styled-content style="fixed-case">PCR</jats:styled-content> and luciferase assay, we found that the 5′‐<jats:styled-content style="fixed-case">UTR</jats:styled-content> of <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content> was not involved in the interaction, whereas the spatial interaction between Nup107 protein and <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content> was formed when <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content> passing through the nuclear pore. Functionally, Nup107 overexpression in neonatal rat ventricle myocytes significantly increased the currents of <jats:italic>Scn5a</jats:italic>‐encoded I<jats:sub>Na</jats:sub> channel. Moreover, the close correlation between Nup107 and Nav1.5 protein expression was observed in cardiomycytes and heart tissues subjected to hypoxia and ischaemic insults, suggesting a fast regulation of Nup107 on Nav1.5 channel in cardiac myocytes in a posttranscriptional manner. These findings may provide insights into the emergent control of cardiac electrophysiology through Nup‐mediated modulation of ion channels.</jats:p> |
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| author | Guan, Yi, Gao, Xueting, Tang, Qiuyu, Huang, Lin, Gao, Siyun, Yu, Shuai, Huang, Jiale, Li, Jun, Zhou, Daizhan, Zhang, Yangyang, Shi, Dan, Liang, Dandan, Liu, Yi, Li, Li, Cui, Yingyu, Xu, Liang, Chen, Yi‐Han |
| author_facet | Guan, Yi, Gao, Xueting, Tang, Qiuyu, Huang, Lin, Gao, Siyun, Yu, Shuai, Huang, Jiale, Li, Jun, Zhou, Daizhan, Zhang, Yangyang, Shi, Dan, Liang, Dandan, Liu, Yi, Li, Li, Cui, Yingyu, Xu, Liang, Chen, Yi‐Han, Guan, Yi, Gao, Xueting, Tang, Qiuyu, Huang, Lin, Gao, Siyun, Yu, Shuai, Huang, Jiale, Li, Jun, Zhou, Daizhan, Zhang, Yangyang, Shi, Dan, Liang, Dandan, Liu, Yi, Li, Li, Cui, Yingyu, Xu, Liang, Chen, Yi‐Han |
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| description | <jats:title>Abstract</jats:title><jats:p>Nucleoporins (Nups) are known to be functional in nucleo‐cytoplasmic transport, but the roles of nucleoporins in nonproliferating cells, such as cardiac myocytes, are still poorly understood. In this study, we report that Nup107 regulates cardiac bioelectricity by controlling the nucleo‐cytoplasmic trafficking of <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content>. Overexpression of Nup107 induced the protein expression of <jats:italic>Scn5a</jats:italic> rather than that of other ion channels, with no effects of their <jats:styled-content style="fixed-case">mRNA</jats:styled-content> levels. The analysis for the protein production demonstrated Nup107‐facilitated transport of <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content>. Using <jats:styled-content style="fixed-case">RIP</jats:styled-content>‐<jats:styled-content style="fixed-case">PCR</jats:styled-content> and luciferase assay, we found that the 5′‐<jats:styled-content style="fixed-case">UTR</jats:styled-content> of <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content> was not involved in the interaction, whereas the spatial interaction between Nup107 protein and <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content> was formed when <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content> passing through the nuclear pore. Functionally, Nup107 overexpression in neonatal rat ventricle myocytes significantly increased the currents of <jats:italic>Scn5a</jats:italic>‐encoded I<jats:sub>Na</jats:sub> channel. Moreover, the close correlation between Nup107 and Nav1.5 protein expression was observed in cardiomycytes and heart tissues subjected to hypoxia and ischaemic insults, suggesting a fast regulation of Nup107 on Nav1.5 channel in cardiac myocytes in a posttranscriptional manner. These findings may provide insights into the emergent control of cardiac electrophysiology through Nup‐mediated modulation of ion channels.</jats:p> |
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| imprint | Wiley, 2019 |
| imprint_str_mv | Wiley, 2019 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229 |
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| physical | 1448-1457 |
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| spelling | Guan, Yi Gao, Xueting Tang, Qiuyu Huang, Lin Gao, Siyun Yu, Shuai Huang, Jiale Li, Jun Zhou, Daizhan Zhang, Yangyang Shi, Dan Liang, Dandan Liu, Yi Li, Li Cui, Yingyu Xu, Liang Chen, Yi‐Han 1582-1838 1582-4934 Wiley Cell Biology Molecular Medicine http://dx.doi.org/10.1111/jcmm.14051 <jats:title>Abstract</jats:title><jats:p>Nucleoporins (Nups) are known to be functional in nucleo‐cytoplasmic transport, but the roles of nucleoporins in nonproliferating cells, such as cardiac myocytes, are still poorly understood. In this study, we report that Nup107 regulates cardiac bioelectricity by controlling the nucleo‐cytoplasmic trafficking of <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content>. Overexpression of Nup107 induced the protein expression of <jats:italic>Scn5a</jats:italic> rather than that of other ion channels, with no effects of their <jats:styled-content style="fixed-case">mRNA</jats:styled-content> levels. The analysis for the protein production demonstrated Nup107‐facilitated transport of <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content>. Using <jats:styled-content style="fixed-case">RIP</jats:styled-content>‐<jats:styled-content style="fixed-case">PCR</jats:styled-content> and luciferase assay, we found that the 5′‐<jats:styled-content style="fixed-case">UTR</jats:styled-content> of <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content> was not involved in the interaction, whereas the spatial interaction between Nup107 protein and <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content> was formed when <jats:italic>Scn5a </jats:italic><jats:styled-content style="fixed-case">mRNA</jats:styled-content> passing through the nuclear pore. Functionally, Nup107 overexpression in neonatal rat ventricle myocytes significantly increased the currents of <jats:italic>Scn5a</jats:italic>‐encoded I<jats:sub>Na</jats:sub> channel. Moreover, the close correlation between Nup107 and Nav1.5 protein expression was observed in cardiomycytes and heart tissues subjected to hypoxia and ischaemic insults, suggesting a fast regulation of Nup107 on Nav1.5 channel in cardiac myocytes in a posttranscriptional manner. These findings may provide insights into the emergent control of cardiac electrophysiology through Nup‐mediated modulation of ion channels.</jats:p> Nucleoporin 107 facilitates the nuclear export of <i>Scn5a </i><scp>mRNA</scp> to regulate cardiac bioelectricity Journal of Cellular and Molecular Medicine |
| spellingShingle | Guan, Yi, Gao, Xueting, Tang, Qiuyu, Huang, Lin, Gao, Siyun, Yu, Shuai, Huang, Jiale, Li, Jun, Zhou, Daizhan, Zhang, Yangyang, Shi, Dan, Liang, Dandan, Liu, Yi, Li, Li, Cui, Yingyu, Xu, Liang, Chen, Yi‐Han, Journal of Cellular and Molecular Medicine, Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity, Cell Biology, Molecular Medicine |
| title | Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity |
| title_full | Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity |
| title_fullStr | Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity |
| title_full_unstemmed | Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity |
| title_short | Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity |
| title_sort | nucleoporin 107 facilitates the nuclear export of <i>scn5a </i><scp>mrna</scp> to regulate cardiac bioelectricity |
| title_unstemmed | Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity |
| topic | Cell Biology, Molecular Medicine |
| url | http://dx.doi.org/10.1111/jcmm.14051 |