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Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules
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| Zeitschriftentitel: | Allergy |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Allergy, 45, 1990, 4, S. 262-267 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Kurosawa, M. Uno, D. Hanawa, K. Kobayashi, S. Kurosawa, M. Uno, D. Hanawa, K. Kobayashi, S. |
|---|---|
| author |
Kurosawa, M. Uno, D. Hanawa, K. Kobayashi, S. |
| spellingShingle |
Kurosawa, M. Uno, D. Hanawa, K. Kobayashi, S. Allergy Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules Immunology Immunology and Allergy |
| author_sort |
kurosawa, m. |
| spelling |
Kurosawa, M. Uno, D. Hanawa, K. Kobayashi, S. 0105-4538 1398-9995 Wiley Immunology Immunology and Allergy http://dx.doi.org/10.1111/j.1398-9995.1990.tb00494.x <jats:p>Rat mast cell granules were obtained by homogenization of highly purified rat mast cells and isolated in a Percoll gradient. Diphosphoinositide (DPI) synthesis in rat mast cell granules was assayed by measuring the incorporation of <jats:sup>32</jats:sup>P from [y<jats:sup>32</jats:sup>P] ATP into DPI in the absence of exogenous phosphatidylinositol (PI). Lipids were isolated with methanol/ chloroform/HCl and were separated by thin‐layer chromatography on oxalic acid impregnated silica gel plates. DPI areas were identified by staining with iodine, scraped and measured for <jats:sup>32</jats:sup>P radioactivity. The addition of polyamines, spermine and spermidine, to the granules caused an increase of DPI synthesis, which can be catalyzed by PI kinase. This effect of polyamines in the DPI synthesis was in a dose‐dependent manner and maximal effects were observed at 1 mM spermine and 10 mM spermidine, respectively.</jats:p> Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules Allergy |
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| title |
Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| title_unstemmed |
Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| title_full |
Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| title_fullStr |
Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| title_full_unstemmed |
Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| title_short |
Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| title_sort |
polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| topic |
Immunology Immunology and Allergy |
| url |
http://dx.doi.org/10.1111/j.1398-9995.1990.tb00494.x |
| publishDate |
1990 |
| physical |
262-267 |
| description |
<jats:p>Rat mast cell granules were obtained by homogenization of highly purified rat mast cells and isolated in a Percoll gradient. Diphosphoinositide (DPI) synthesis in rat mast cell granules was assayed by measuring the incorporation of <jats:sup>32</jats:sup>P from [y<jats:sup>32</jats:sup>P] ATP into DPI in the absence of exogenous phosphatidylinositol (PI). Lipids were isolated with methanol/ chloroform/HCl and were separated by thin‐layer chromatography on oxalic acid impregnated silica gel plates. DPI areas were identified by staining with iodine, scraped and measured for <jats:sup>32</jats:sup>P radioactivity. The addition of polyamines, spermine and spermidine, to the granules caused an increase of DPI synthesis, which can be catalyzed by PI kinase. This effect of polyamines in the DPI synthesis was in a dose‐dependent manner and maximal effects were observed at 1 mM spermine and 10 mM spermidine, respectively.</jats:p> |
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| author | Kurosawa, M., Uno, D., Hanawa, K., Kobayashi, S. |
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| description | <jats:p>Rat mast cell granules were obtained by homogenization of highly purified rat mast cells and isolated in a Percoll gradient. Diphosphoinositide (DPI) synthesis in rat mast cell granules was assayed by measuring the incorporation of <jats:sup>32</jats:sup>P from [y<jats:sup>32</jats:sup>P] ATP into DPI in the absence of exogenous phosphatidylinositol (PI). Lipids were isolated with methanol/ chloroform/HCl and were separated by thin‐layer chromatography on oxalic acid impregnated silica gel plates. DPI areas were identified by staining with iodine, scraped and measured for <jats:sup>32</jats:sup>P radioactivity. The addition of polyamines, spermine and spermidine, to the granules caused an increase of DPI synthesis, which can be catalyzed by PI kinase. This effect of polyamines in the DPI synthesis was in a dose‐dependent manner and maximal effects were observed at 1 mM spermine and 10 mM spermidine, respectively.</jats:p> |
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| imprint_str_mv | Wiley, 1990 |
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| spelling | Kurosawa, M. Uno, D. Hanawa, K. Kobayashi, S. 0105-4538 1398-9995 Wiley Immunology Immunology and Allergy http://dx.doi.org/10.1111/j.1398-9995.1990.tb00494.x <jats:p>Rat mast cell granules were obtained by homogenization of highly purified rat mast cells and isolated in a Percoll gradient. Diphosphoinositide (DPI) synthesis in rat mast cell granules was assayed by measuring the incorporation of <jats:sup>32</jats:sup>P from [y<jats:sup>32</jats:sup>P] ATP into DPI in the absence of exogenous phosphatidylinositol (PI). Lipids were isolated with methanol/ chloroform/HCl and were separated by thin‐layer chromatography on oxalic acid impregnated silica gel plates. DPI areas were identified by staining with iodine, scraped and measured for <jats:sup>32</jats:sup>P radioactivity. The addition of polyamines, spermine and spermidine, to the granules caused an increase of DPI synthesis, which can be catalyzed by PI kinase. This effect of polyamines in the DPI synthesis was in a dose‐dependent manner and maximal effects were observed at 1 mM spermine and 10 mM spermidine, respectively.</jats:p> Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules Allergy |
| spellingShingle | Kurosawa, M., Uno, D., Hanawa, K., Kobayashi, S., Allergy, Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules, Immunology, Immunology and Allergy |
| title | Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| title_full | Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| title_fullStr | Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| title_full_unstemmed | Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| title_short | Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| title_sort | polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| title_unstemmed | Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules |
| topic | Immunology, Immunology and Allergy |
| url | http://dx.doi.org/10.1111/j.1398-9995.1990.tb00494.x |