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Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay
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| Zeitschriftentitel: | Scandinavian Journal of Immunology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Scandinavian Journal of Immunology, 11, 1980, 3, S. 283-289 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
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| author_facet |
FREIJD, A. KUNORI, T. FREIJD, A. KUNORI, T. |
|---|---|
| author |
FREIJD, A. KUNORI, T. |
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FREIJD, A. KUNORI, T. Scandinavian Journal of Immunology Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay Immunology General Medicine |
| author_sort |
freijd, a. |
| spelling |
FREIJD, A. KUNORI, T. 0300-9475 1365-3083 Wiley Immunology General Medicine http://dx.doi.org/10.1111/j.1365-3083.1980.tb00236.x <jats:p>A sizable proportion of fresh human blood and adenoid lymphocytes were found to produce antibodies when tested in the protein‐A plaque assay of Gronowicz <jats:italic>et al.</jats:italic> Plaque formation was not caused by release of passively adsorbed immunoglobulin but depended on active‐ immunoglobulin secretion We have Investigated optimal conditions for plaque formation In this test system. Good reproducibility is obtained only if Cells to be tested are kept in the cold before assay, since the antibody‐forming capacity of these cells is rapidly exhausted at temperatures above 2°C. In blood lymphocytes the number of IgA‐producing cells exceeds those of IgG and IgM, whereat in the adenoid IgG plaque‐forming cell dominate. Studies on the on of this method for the monitoring of immune responses are in progress.</jats:p> Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay Scandinavian Journal of Immunology |
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Wiley, 1980 |
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Scandinavian Journal of Immunology |
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| title |
Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay |
| title_unstemmed |
Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay |
| title_full |
Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay |
| title_fullStr |
Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay |
| title_full_unstemmed |
Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay |
| title_short |
Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay |
| title_sort |
spontaneous plaque‐forming human lymphocytes detected with the protein‐a plaque assay |
| topic |
Immunology General Medicine |
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http://dx.doi.org/10.1111/j.1365-3083.1980.tb00236.x |
| publishDate |
1980 |
| physical |
283-289 |
| description |
<jats:p>A sizable proportion of fresh human blood and adenoid lymphocytes were found to produce antibodies when tested in the protein‐A plaque assay of Gronowicz <jats:italic>et al.</jats:italic> Plaque formation was not caused by release of passively adsorbed immunoglobulin but depended on active‐ immunoglobulin secretion We have Investigated optimal conditions for plaque formation In this test system. Good reproducibility is obtained only if Cells to be tested are kept in the cold before assay, since the antibody‐forming capacity of these cells is rapidly exhausted at temperatures above 2°C. In blood lymphocytes the number of IgA‐producing cells exceeds those of IgG and IgM, whereat in the adenoid IgG plaque‐forming cell dominate. Studies on the on of this method for the monitoring of immune responses are in progress.</jats:p> |
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| description | <jats:p>A sizable proportion of fresh human blood and adenoid lymphocytes were found to produce antibodies when tested in the protein‐A plaque assay of Gronowicz <jats:italic>et al.</jats:italic> Plaque formation was not caused by release of passively adsorbed immunoglobulin but depended on active‐ immunoglobulin secretion We have Investigated optimal conditions for plaque formation In this test system. Good reproducibility is obtained only if Cells to be tested are kept in the cold before assay, since the antibody‐forming capacity of these cells is rapidly exhausted at temperatures above 2°C. In blood lymphocytes the number of IgA‐producing cells exceeds those of IgG and IgM, whereat in the adenoid IgG plaque‐forming cell dominate. Studies on the on of this method for the monitoring of immune responses are in progress.</jats:p> |
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| imprint_str_mv | Wiley, 1980 |
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| series | Scandinavian Journal of Immunology |
| source_id | 49 |
| spelling | FREIJD, A. KUNORI, T. 0300-9475 1365-3083 Wiley Immunology General Medicine http://dx.doi.org/10.1111/j.1365-3083.1980.tb00236.x <jats:p>A sizable proportion of fresh human blood and adenoid lymphocytes were found to produce antibodies when tested in the protein‐A plaque assay of Gronowicz <jats:italic>et al.</jats:italic> Plaque formation was not caused by release of passively adsorbed immunoglobulin but depended on active‐ immunoglobulin secretion We have Investigated optimal conditions for plaque formation In this test system. Good reproducibility is obtained only if Cells to be tested are kept in the cold before assay, since the antibody‐forming capacity of these cells is rapidly exhausted at temperatures above 2°C. In blood lymphocytes the number of IgA‐producing cells exceeds those of IgG and IgM, whereat in the adenoid IgG plaque‐forming cell dominate. Studies on the on of this method for the monitoring of immune responses are in progress.</jats:p> Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay Scandinavian Journal of Immunology |
| spellingShingle | FREIJD, A., KUNORI, T., Scandinavian Journal of Immunology, Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay, Immunology, General Medicine |
| title | Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay |
| title_full | Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay |
| title_fullStr | Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay |
| title_full_unstemmed | Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay |
| title_short | Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay |
| title_sort | spontaneous plaque‐forming human lymphocytes detected with the protein‐a plaque assay |
| title_unstemmed | Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay |
| topic | Immunology, General Medicine |
| url | http://dx.doi.org/10.1111/j.1365-3083.1980.tb00236.x |