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Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations
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| Zeitschriftentitel: | Scandinavian Journal of Immunology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Scandinavian Journal of Immunology, 11, 1980, 2, S. 137-145 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Kunori, T. RINGDÉN, O. Kunori, T. RINGDÉN, O. |
|---|---|
| author |
Kunori, T. RINGDÉN, O. |
| spellingShingle |
Kunori, T. RINGDÉN, O. Scandinavian Journal of Immunology Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations Immunology General Medicine |
| author_sort |
kunori, t. |
| spelling |
Kunori, T. RINGDÉN, O. 0300-9475 1365-3083 Wiley Immunology General Medicine http://dx.doi.org/10.1111/j.1365-3083.1980.tb00219.x <jats:p>Lipopolysaccharide (LPS)‐induced human lymphocyte activation was analysed with regard to DNA synthesis and antibody secretion. Antibody secretion was measured as plaque‐forming cells (PFC) against fluorescein isothiocyanate (FITC)‐coupled sheep erythrocytes (SRBC) or protein‐A‐coupled SRBC in the presence of developing antiserum against human IgG, IgA and IgM subclasses. By co‐culturing lymphocytes with various concentrations of mitomycin, without impairing cell viability, inhibition of DNA synthesis and antibody secretion by LPS was correlated. Antibody secretion against FITC‐SRBC or protein A‐SRBC was not stimulated by LPS in B cells enriched by elimination of SRBC rosette‐forming cells (E‐RFC). In mixtures of enriched T and B cells the number of PFC was much lower than in unseparated lymphocytes, but the highest number of PFC was seen in the enriched E‐RFC. However, DNA synthesis by LPS was induced in enriched B cells and not in enriched T cells. The highest DNA synthesis was seen for unseparated lymphocytes. Furthermore, autoradiography studies and experiments in which activated lymphocytes were separated revealed that LPS predominantly stimulated DNA synthesis in non‐E‐RFC, presumably B cells and only activated a small proportion of E‐RFC. Finally, antibody secretion induced by LPS was unchanged following iron treatment but was inhibited by cells adherent to plastic.</jats:p> Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations Scandinavian Journal of Immunology |
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Wiley, 1980 |
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Scandinavian Journal of Immunology |
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49 |
| title |
Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations |
| title_unstemmed |
Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations |
| title_full |
Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations |
| title_fullStr |
Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations |
| title_full_unstemmed |
Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations |
| title_short |
Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations |
| title_sort |
antibody secretion and dna synthesis induced by lipopolysaccharide in enriched human lymphocyte subpopulations |
| topic |
Immunology General Medicine |
| url |
http://dx.doi.org/10.1111/j.1365-3083.1980.tb00219.x |
| publishDate |
1980 |
| physical |
137-145 |
| description |
<jats:p>Lipopolysaccharide (LPS)‐induced human lymphocyte activation was analysed with regard to DNA synthesis and antibody secretion. Antibody secretion was measured as plaque‐forming cells (PFC) against fluorescein isothiocyanate (FITC)‐coupled sheep erythrocytes (SRBC) or protein‐A‐coupled SRBC in the presence of developing antiserum against human IgG, IgA and IgM subclasses. By co‐culturing lymphocytes with various concentrations of mitomycin, without impairing cell viability, inhibition of DNA synthesis and antibody secretion by LPS was correlated. Antibody secretion against FITC‐SRBC or protein A‐SRBC was not stimulated by LPS in B cells enriched by elimination of SRBC rosette‐forming cells (E‐RFC). In mixtures of enriched T and B cells the number of PFC was much lower than in unseparated lymphocytes, but the highest number of PFC was seen in the enriched E‐RFC. However, DNA synthesis by LPS was induced in enriched B cells and not in enriched T cells. The highest DNA synthesis was seen for unseparated lymphocytes. Furthermore, autoradiography studies and experiments in which activated lymphocytes were separated revealed that LPS predominantly stimulated DNA synthesis in non‐E‐RFC, presumably B cells and only activated a small proportion of E‐RFC. Finally, antibody secretion induced by LPS was unchanged following iron treatment but was inhibited by cells adherent to plastic.</jats:p> |
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| author | Kunori, T., RINGDÉN, O. |
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| description | <jats:p>Lipopolysaccharide (LPS)‐induced human lymphocyte activation was analysed with regard to DNA synthesis and antibody secretion. Antibody secretion was measured as plaque‐forming cells (PFC) against fluorescein isothiocyanate (FITC)‐coupled sheep erythrocytes (SRBC) or protein‐A‐coupled SRBC in the presence of developing antiserum against human IgG, IgA and IgM subclasses. By co‐culturing lymphocytes with various concentrations of mitomycin, without impairing cell viability, inhibition of DNA synthesis and antibody secretion by LPS was correlated. Antibody secretion against FITC‐SRBC or protein A‐SRBC was not stimulated by LPS in B cells enriched by elimination of SRBC rosette‐forming cells (E‐RFC). In mixtures of enriched T and B cells the number of PFC was much lower than in unseparated lymphocytes, but the highest number of PFC was seen in the enriched E‐RFC. However, DNA synthesis by LPS was induced in enriched B cells and not in enriched T cells. The highest DNA synthesis was seen for unseparated lymphocytes. Furthermore, autoradiography studies and experiments in which activated lymphocytes were separated revealed that LPS predominantly stimulated DNA synthesis in non‐E‐RFC, presumably B cells and only activated a small proportion of E‐RFC. Finally, antibody secretion induced by LPS was unchanged following iron treatment but was inhibited by cells adherent to plastic.</jats:p> |
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| imprint | Wiley, 1980 |
| imprint_str_mv | Wiley, 1980 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229 |
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| series | Scandinavian Journal of Immunology |
| source_id | 49 |
| spelling | Kunori, T. RINGDÉN, O. 0300-9475 1365-3083 Wiley Immunology General Medicine http://dx.doi.org/10.1111/j.1365-3083.1980.tb00219.x <jats:p>Lipopolysaccharide (LPS)‐induced human lymphocyte activation was analysed with regard to DNA synthesis and antibody secretion. Antibody secretion was measured as plaque‐forming cells (PFC) against fluorescein isothiocyanate (FITC)‐coupled sheep erythrocytes (SRBC) or protein‐A‐coupled SRBC in the presence of developing antiserum against human IgG, IgA and IgM subclasses. By co‐culturing lymphocytes with various concentrations of mitomycin, without impairing cell viability, inhibition of DNA synthesis and antibody secretion by LPS was correlated. Antibody secretion against FITC‐SRBC or protein A‐SRBC was not stimulated by LPS in B cells enriched by elimination of SRBC rosette‐forming cells (E‐RFC). In mixtures of enriched T and B cells the number of PFC was much lower than in unseparated lymphocytes, but the highest number of PFC was seen in the enriched E‐RFC. However, DNA synthesis by LPS was induced in enriched B cells and not in enriched T cells. The highest DNA synthesis was seen for unseparated lymphocytes. Furthermore, autoradiography studies and experiments in which activated lymphocytes were separated revealed that LPS predominantly stimulated DNA synthesis in non‐E‐RFC, presumably B cells and only activated a small proportion of E‐RFC. Finally, antibody secretion induced by LPS was unchanged following iron treatment but was inhibited by cells adherent to plastic.</jats:p> Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations Scandinavian Journal of Immunology |
| spellingShingle | Kunori, T., RINGDÉN, O., Scandinavian Journal of Immunology, Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations, Immunology, General Medicine |
| title | Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations |
| title_full | Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations |
| title_fullStr | Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations |
| title_full_unstemmed | Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations |
| title_short | Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations |
| title_sort | antibody secretion and dna synthesis induced by lipopolysaccharide in enriched human lymphocyte subpopulations |
| title_unstemmed | Antibody Secretion and DNA Synthesis Induced by Lipopolysaccharide in Enriched Human Lymphocyte Subpopulations |
| topic | Immunology, General Medicine |
| url | http://dx.doi.org/10.1111/j.1365-3083.1980.tb00219.x |