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Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses
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| Zeitschriftentitel: | Plant, Cell & Environment |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Plant, Cell & Environment, 27, 2004, 7, S. 947-957 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
KOMATSU, S. YANG, G. HAYASHI, N. KAKU, H. UMEMURA, K. IWASAKI, Y. KOMATSU, S. YANG, G. HAYASHI, N. KAKU, H. UMEMURA, K. IWASAKI, Y. |
|---|---|
| author |
KOMATSU, S. YANG, G. HAYASHI, N. KAKU, H. UMEMURA, K. IWASAKI, Y. |
| spellingShingle |
KOMATSU, S. YANG, G. HAYASHI, N. KAKU, H. UMEMURA, K. IWASAKI, Y. Plant, Cell & Environment Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses Plant Science Physiology |
| author_sort |
komatsu, s. |
| spelling |
KOMATSU, S. YANG, G. HAYASHI, N. KAKU, H. UMEMURA, K. IWASAKI, Y. 0140-7791 1365-3040 Wiley Plant Science Physiology http://dx.doi.org/10.1111/j.1365-3040.2004.01202.x <jats:title>ABSTRACT</jats:title><jats:p>The rice dwarf1 (<jats:italic>d1</jats:italic>) mutant, which is deficient in an <jats:italic>α</jats:italic> subunit (G<jats:italic>α</jats:italic>) of heterotrimeric G protein, was used to obtain specific evidence on the functions of G<jats:italic>α</jats:italic> protein in defence signalling in rice. Using proteome analysis, a probenazole‐inducible protein (PBZ1) was detected in the cytosolic fraction of leaf blade of the wild type, but not the <jats:italic>d1</jats:italic> mutant. After treatment with probenazol, PBZ1 reached maximal levels at 72 h in the wild type but 96 h in the <jats:italic>d1</jats:italic> mutant. The induction of PBZ1 by probenazole treatment was inhibited by protein kinase inhibitors. A 48‐kDa putative mitogen‐activated protein kinase (MAPK) and a 55‐kDa putative Ca<jats:sup>2+</jats:sup>‐dependent protein kinase (CDPK) showed lower activities in the cytosolic fraction of the <jats:italic>d1</jats:italic> mutant than that of the wild type. The activities of these protein kinases were enhanced at 24 h in the wild type and 48 h in the <jats:italic>d1</jats:italic> mutant after probenazole treatment. Although the <jats:italic>d1</jats:italic> mutant responded to the rice blast fungus similarly to the wild type, the <jats:italic>d1</jats:italic> mutant developed rice blight symptoms earlier than the wild type when infected with <jats:italic>Xoo</jats:italic>. In addition, the blight symptoms were more severe on the mutant than on the wild type, and wilting was frequently observed in the <jats:italic>d1</jats:italic> mutant. Furthermore, induction by the bacterial infection of the 48‐kDa putative MAPK and PBZ1 was delayed by 2 and 4 d, respectively, in the <jats:italic>d1</jats:italic> mutant compared with the wild type. These results indicate that the G<jats:italic>α</jats:italic> protein plays a role in the induction of PBZ1 and protein kinases by probenazole and <jats:italic>Xoo</jats:italic>, and suggest that the 48‐kDa putative MAPK may be involved in a signalling pathway for resistance to bacterial infection.</jats:p> Alterations by a defect in a rice G protein <i>α</i> subunit in probenazole and pathogen‐induced responses Plant, Cell & Environment |
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Wiley, 2004 |
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Plant, Cell & Environment |
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| title |
Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses |
| title_unstemmed |
Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses |
| title_full |
Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses |
| title_fullStr |
Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses |
| title_full_unstemmed |
Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses |
| title_short |
Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses |
| title_sort |
alterations by a defect in a rice g protein <i>α</i> subunit in probenazole and pathogen‐induced responses |
| topic |
Plant Science Physiology |
| url |
http://dx.doi.org/10.1111/j.1365-3040.2004.01202.x |
| publishDate |
2004 |
| physical |
947-957 |
| description |
<jats:title>ABSTRACT</jats:title><jats:p>The rice dwarf1 (<jats:italic>d1</jats:italic>) mutant, which is deficient in an <jats:italic>α</jats:italic> subunit (G<jats:italic>α</jats:italic>) of heterotrimeric G protein, was used to obtain specific evidence on the functions of G<jats:italic>α</jats:italic> protein in defence signalling in rice. Using proteome analysis, a probenazole‐inducible protein (PBZ1) was detected in the cytosolic fraction of leaf blade of the wild type, but not the <jats:italic>d1</jats:italic> mutant. After treatment with probenazol, PBZ1 reached maximal levels at 72 h in the wild type but 96 h in the <jats:italic>d1</jats:italic> mutant. The induction of PBZ1 by probenazole treatment was inhibited by protein kinase inhibitors. A 48‐kDa putative mitogen‐activated protein kinase (MAPK) and a 55‐kDa putative Ca<jats:sup>2+</jats:sup>‐dependent protein kinase (CDPK) showed lower activities in the cytosolic fraction of the <jats:italic>d1</jats:italic> mutant than that of the wild type. The activities of these protein kinases were enhanced at 24 h in the wild type and 48 h in the <jats:italic>d1</jats:italic> mutant after probenazole treatment. Although the <jats:italic>d1</jats:italic> mutant responded to the rice blast fungus similarly to the wild type, the <jats:italic>d1</jats:italic> mutant developed rice blight symptoms earlier than the wild type when infected with <jats:italic>Xoo</jats:italic>. In addition, the blight symptoms were more severe on the mutant than on the wild type, and wilting was frequently observed in the <jats:italic>d1</jats:italic> mutant. Furthermore, induction by the bacterial infection of the 48‐kDa putative MAPK and PBZ1 was delayed by 2 and 4 d, respectively, in the <jats:italic>d1</jats:italic> mutant compared with the wild type. These results indicate that the G<jats:italic>α</jats:italic> protein plays a role in the induction of PBZ1 and protein kinases by probenazole and <jats:italic>Xoo</jats:italic>, and suggest that the 48‐kDa putative MAPK may be involved in a signalling pathway for resistance to bacterial infection.</jats:p> |
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| author | KOMATSU, S., YANG, G., HAYASHI, N., KAKU, H., UMEMURA, K., IWASAKI, Y. |
| author_facet | KOMATSU, S., YANG, G., HAYASHI, N., KAKU, H., UMEMURA, K., IWASAKI, Y., KOMATSU, S., YANG, G., HAYASHI, N., KAKU, H., UMEMURA, K., IWASAKI, Y. |
| author_sort | komatsu, s. |
| container_issue | 7 |
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| description | <jats:title>ABSTRACT</jats:title><jats:p>The rice dwarf1 (<jats:italic>d1</jats:italic>) mutant, which is deficient in an <jats:italic>α</jats:italic> subunit (G<jats:italic>α</jats:italic>) of heterotrimeric G protein, was used to obtain specific evidence on the functions of G<jats:italic>α</jats:italic> protein in defence signalling in rice. Using proteome analysis, a probenazole‐inducible protein (PBZ1) was detected in the cytosolic fraction of leaf blade of the wild type, but not the <jats:italic>d1</jats:italic> mutant. After treatment with probenazol, PBZ1 reached maximal levels at 72 h in the wild type but 96 h in the <jats:italic>d1</jats:italic> mutant. The induction of PBZ1 by probenazole treatment was inhibited by protein kinase inhibitors. A 48‐kDa putative mitogen‐activated protein kinase (MAPK) and a 55‐kDa putative Ca<jats:sup>2+</jats:sup>‐dependent protein kinase (CDPK) showed lower activities in the cytosolic fraction of the <jats:italic>d1</jats:italic> mutant than that of the wild type. The activities of these protein kinases were enhanced at 24 h in the wild type and 48 h in the <jats:italic>d1</jats:italic> mutant after probenazole treatment. Although the <jats:italic>d1</jats:italic> mutant responded to the rice blast fungus similarly to the wild type, the <jats:italic>d1</jats:italic> mutant developed rice blight symptoms earlier than the wild type when infected with <jats:italic>Xoo</jats:italic>. In addition, the blight symptoms were more severe on the mutant than on the wild type, and wilting was frequently observed in the <jats:italic>d1</jats:italic> mutant. Furthermore, induction by the bacterial infection of the 48‐kDa putative MAPK and PBZ1 was delayed by 2 and 4 d, respectively, in the <jats:italic>d1</jats:italic> mutant compared with the wild type. These results indicate that the G<jats:italic>α</jats:italic> protein plays a role in the induction of PBZ1 and protein kinases by probenazole and <jats:italic>Xoo</jats:italic>, and suggest that the 48‐kDa putative MAPK may be involved in a signalling pathway for resistance to bacterial infection.</jats:p> |
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| imprint | Wiley, 2004 |
| imprint_str_mv | Wiley, 2004 |
| institution | DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11 |
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| spelling | KOMATSU, S. YANG, G. HAYASHI, N. KAKU, H. UMEMURA, K. IWASAKI, Y. 0140-7791 1365-3040 Wiley Plant Science Physiology http://dx.doi.org/10.1111/j.1365-3040.2004.01202.x <jats:title>ABSTRACT</jats:title><jats:p>The rice dwarf1 (<jats:italic>d1</jats:italic>) mutant, which is deficient in an <jats:italic>α</jats:italic> subunit (G<jats:italic>α</jats:italic>) of heterotrimeric G protein, was used to obtain specific evidence on the functions of G<jats:italic>α</jats:italic> protein in defence signalling in rice. Using proteome analysis, a probenazole‐inducible protein (PBZ1) was detected in the cytosolic fraction of leaf blade of the wild type, but not the <jats:italic>d1</jats:italic> mutant. After treatment with probenazol, PBZ1 reached maximal levels at 72 h in the wild type but 96 h in the <jats:italic>d1</jats:italic> mutant. The induction of PBZ1 by probenazole treatment was inhibited by protein kinase inhibitors. A 48‐kDa putative mitogen‐activated protein kinase (MAPK) and a 55‐kDa putative Ca<jats:sup>2+</jats:sup>‐dependent protein kinase (CDPK) showed lower activities in the cytosolic fraction of the <jats:italic>d1</jats:italic> mutant than that of the wild type. The activities of these protein kinases were enhanced at 24 h in the wild type and 48 h in the <jats:italic>d1</jats:italic> mutant after probenazole treatment. Although the <jats:italic>d1</jats:italic> mutant responded to the rice blast fungus similarly to the wild type, the <jats:italic>d1</jats:italic> mutant developed rice blight symptoms earlier than the wild type when infected with <jats:italic>Xoo</jats:italic>. In addition, the blight symptoms were more severe on the mutant than on the wild type, and wilting was frequently observed in the <jats:italic>d1</jats:italic> mutant. Furthermore, induction by the bacterial infection of the 48‐kDa putative MAPK and PBZ1 was delayed by 2 and 4 d, respectively, in the <jats:italic>d1</jats:italic> mutant compared with the wild type. These results indicate that the G<jats:italic>α</jats:italic> protein plays a role in the induction of PBZ1 and protein kinases by probenazole and <jats:italic>Xoo</jats:italic>, and suggest that the 48‐kDa putative MAPK may be involved in a signalling pathway for resistance to bacterial infection.</jats:p> Alterations by a defect in a rice G protein <i>α</i> subunit in probenazole and pathogen‐induced responses Plant, Cell & Environment |
| spellingShingle | KOMATSU, S., YANG, G., HAYASHI, N., KAKU, H., UMEMURA, K., IWASAKI, Y., Plant, Cell & Environment, Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses, Plant Science, Physiology |
| title | Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses |
| title_full | Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses |
| title_fullStr | Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses |
| title_full_unstemmed | Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses |
| title_short | Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses |
| title_sort | alterations by a defect in a rice g protein <i>α</i> subunit in probenazole and pathogen‐induced responses |
| title_unstemmed | Alterations by a defect in a rice G protein α subunit in probenazole and pathogen‐induced responses |
| topic | Plant Science, Physiology |
| url | http://dx.doi.org/10.1111/j.1365-3040.2004.01202.x |