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Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS)
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| Zeitschriftentitel: | Clinical and Experimental Immunology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Clinical and Experimental Immunology, 91, 2008, 3, S. 526-531 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Oxford University Press (OUP)
|
| Schlagwörter: |
| author_facet |
CONTI, P PANARA, M R BARBACANE, R C BONGRAZIO, M DEMPSEY, R A REALE, M CONTI, P PANARA, M R BARBACANE, R C BONGRAZIO, M DEMPSEY, R A REALE, M |
|---|---|
| author |
CONTI, P PANARA, M R BARBACANE, R C BONGRAZIO, M DEMPSEY, R A REALE, M |
| spellingShingle |
CONTI, P PANARA, M R BARBACANE, R C BONGRAZIO, M DEMPSEY, R A REALE, M Clinical and Experimental Immunology Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) Immunology Immunology and Allergy |
| author_sort |
conti, p |
| spelling |
CONTI, P PANARA, M R BARBACANE, R C BONGRAZIO, M DEMPSEY, R A REALE, M 0009-9104 1365-2249 Oxford University Press (OUP) Immunology Immunology and Allergy http://dx.doi.org/10.1111/j.1365-2249.1993.tb05936.x <jats:title>SUMMARY</jats:title><jats:p>The effect of human recombinant IL-1 receptor antagonist (hrIL-1Ra) on leukotriene B4 (LTB4) release was investigated in activated human monocyte cultures. To stimulate LTB4 generation, LPS was used as an agonist. Detection was performed with the highly sensitive radioimmunoassay method. The cells were treated with scalar concentrations using LPS at 1–1000 ng/ml for different periods of time. The greater LTB4 stimulation was found at LPS 100 ng/ml for 18 h incubation time. Preincubation of monocytes with cytochalasin B (CB) (5 μg/ml) for 15 min augmented the release of LTB4 when LPS was used. A dose-dependent inhibition was found when human monocytes were pretreated for 10 min with hrlL-l Ra at different concentrations (0·25–250 ng/ml) and then treated with LPS 100 ng/ml for 18 h. Maximum inhibition was observed at the highest concentration of hrlL-1 Ra (250 ng/ml). Macrophages treated with a non-selective 5-lipoxygcnasc inhibitor, nordihydro-guaiaretic acid (NDGA), used at 10 μm, added 15 min before LPS 100 ng/ml, produce a dose-dependent inhibition of LTB4. Cells pretreated with arachidonic acid, at various concentrations (10−9− 10−5 m) for 10 min and then treated with LPS 100 ng/ml for 18 h, were also inhibited in a dose-dependent manner by hrIL-1 Ra in their production of LTB4. The inhibition of LTB4 release by hrlL-I Ra, in LPS-stimulated human monocytes, may suggest an important modulatory role for this new cytokine (monokine) in inflammation and immunity and may hold future therapeutic implications for diseases involving LTB4 as a mediator.</jats:p> Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) Clinical and Experimental Immunology |
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| title |
Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) |
| title_unstemmed |
Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) |
| title_full |
Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) |
| title_fullStr |
Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) |
| title_full_unstemmed |
Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) |
| title_short |
Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) |
| title_sort |
human recombinant il-1 receptor antagonist (il-ira) inhibits leukotriene b4 generation from human monocyte suspensions stimulated by lipopolysaccharide (lps) |
| topic |
Immunology Immunology and Allergy |
| url |
http://dx.doi.org/10.1111/j.1365-2249.1993.tb05936.x |
| publishDate |
2008 |
| physical |
526-531 |
| description |
<jats:title>SUMMARY</jats:title><jats:p>The effect of human recombinant IL-1 receptor antagonist (hrIL-1Ra) on leukotriene B4 (LTB4) release was investigated in activated human monocyte cultures. To stimulate LTB4 generation, LPS was used as an agonist. Detection was performed with the highly sensitive radioimmunoassay method. The cells were treated with scalar concentrations using LPS at 1–1000 ng/ml for different periods of time. The greater LTB4 stimulation was found at LPS 100 ng/ml for 18 h incubation time. Preincubation of monocytes with cytochalasin B (CB) (5 μg/ml) for 15 min augmented the release of LTB4 when LPS was used. A dose-dependent inhibition was found when human monocytes were pretreated for 10 min with hrlL-l Ra at different concentrations (0·25–250 ng/ml) and then treated with LPS 100 ng/ml for 18 h. Maximum inhibition was observed at the highest concentration of hrlL-1 Ra (250 ng/ml). Macrophages treated with a non-selective 5-lipoxygcnasc inhibitor, nordihydro-guaiaretic acid (NDGA), used at 10 μm, added 15 min before LPS 100 ng/ml, produce a dose-dependent inhibition of LTB4. Cells pretreated with arachidonic acid, at various concentrations (10−9− 10−5 m) for 10 min and then treated with LPS 100 ng/ml for 18 h, were also inhibited in a dose-dependent manner by hrIL-1 Ra in their production of LTB4. The inhibition of LTB4 release by hrlL-I Ra, in LPS-stimulated human monocytes, may suggest an important modulatory role for this new cytokine (monokine) in inflammation and immunity and may hold future therapeutic implications for diseases involving LTB4 as a mediator.</jats:p> |
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| author | CONTI, P, PANARA, M R, BARBACANE, R C, BONGRAZIO, M, DEMPSEY, R A, REALE, M |
| author_facet | CONTI, P, PANARA, M R, BARBACANE, R C, BONGRAZIO, M, DEMPSEY, R A, REALE, M, CONTI, P, PANARA, M R, BARBACANE, R C, BONGRAZIO, M, DEMPSEY, R A, REALE, M |
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| description | <jats:title>SUMMARY</jats:title><jats:p>The effect of human recombinant IL-1 receptor antagonist (hrIL-1Ra) on leukotriene B4 (LTB4) release was investigated in activated human monocyte cultures. To stimulate LTB4 generation, LPS was used as an agonist. Detection was performed with the highly sensitive radioimmunoassay method. The cells were treated with scalar concentrations using LPS at 1–1000 ng/ml for different periods of time. The greater LTB4 stimulation was found at LPS 100 ng/ml for 18 h incubation time. Preincubation of monocytes with cytochalasin B (CB) (5 μg/ml) for 15 min augmented the release of LTB4 when LPS was used. A dose-dependent inhibition was found when human monocytes were pretreated for 10 min with hrlL-l Ra at different concentrations (0·25–250 ng/ml) and then treated with LPS 100 ng/ml for 18 h. Maximum inhibition was observed at the highest concentration of hrlL-1 Ra (250 ng/ml). Macrophages treated with a non-selective 5-lipoxygcnasc inhibitor, nordihydro-guaiaretic acid (NDGA), used at 10 μm, added 15 min before LPS 100 ng/ml, produce a dose-dependent inhibition of LTB4. Cells pretreated with arachidonic acid, at various concentrations (10−9− 10−5 m) for 10 min and then treated with LPS 100 ng/ml for 18 h, were also inhibited in a dose-dependent manner by hrIL-1 Ra in their production of LTB4. The inhibition of LTB4 release by hrlL-I Ra, in LPS-stimulated human monocytes, may suggest an important modulatory role for this new cytokine (monokine) in inflammation and immunity and may hold future therapeutic implications for diseases involving LTB4 as a mediator.</jats:p> |
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| spelling | CONTI, P PANARA, M R BARBACANE, R C BONGRAZIO, M DEMPSEY, R A REALE, M 0009-9104 1365-2249 Oxford University Press (OUP) Immunology Immunology and Allergy http://dx.doi.org/10.1111/j.1365-2249.1993.tb05936.x <jats:title>SUMMARY</jats:title><jats:p>The effect of human recombinant IL-1 receptor antagonist (hrIL-1Ra) on leukotriene B4 (LTB4) release was investigated in activated human monocyte cultures. To stimulate LTB4 generation, LPS was used as an agonist. Detection was performed with the highly sensitive radioimmunoassay method. The cells were treated with scalar concentrations using LPS at 1–1000 ng/ml for different periods of time. The greater LTB4 stimulation was found at LPS 100 ng/ml for 18 h incubation time. Preincubation of monocytes with cytochalasin B (CB) (5 μg/ml) for 15 min augmented the release of LTB4 when LPS was used. A dose-dependent inhibition was found when human monocytes were pretreated for 10 min with hrlL-l Ra at different concentrations (0·25–250 ng/ml) and then treated with LPS 100 ng/ml for 18 h. Maximum inhibition was observed at the highest concentration of hrlL-1 Ra (250 ng/ml). Macrophages treated with a non-selective 5-lipoxygcnasc inhibitor, nordihydro-guaiaretic acid (NDGA), used at 10 μm, added 15 min before LPS 100 ng/ml, produce a dose-dependent inhibition of LTB4. Cells pretreated with arachidonic acid, at various concentrations (10−9− 10−5 m) for 10 min and then treated with LPS 100 ng/ml for 18 h, were also inhibited in a dose-dependent manner by hrIL-1 Ra in their production of LTB4. The inhibition of LTB4 release by hrlL-I Ra, in LPS-stimulated human monocytes, may suggest an important modulatory role for this new cytokine (monokine) in inflammation and immunity and may hold future therapeutic implications for diseases involving LTB4 as a mediator.</jats:p> Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) Clinical and Experimental Immunology |
| spellingShingle | CONTI, P, PANARA, M R, BARBACANE, R C, BONGRAZIO, M, DEMPSEY, R A, REALE, M, Clinical and Experimental Immunology, Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS), Immunology, Immunology and Allergy |
| title | Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) |
| title_full | Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) |
| title_fullStr | Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) |
| title_full_unstemmed | Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) |
| title_short | Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) |
| title_sort | human recombinant il-1 receptor antagonist (il-ira) inhibits leukotriene b4 generation from human monocyte suspensions stimulated by lipopolysaccharide (lps) |
| title_unstemmed | Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS) |
| topic | Immunology, Immunology and Allergy |
| url | http://dx.doi.org/10.1111/j.1365-2249.1993.tb05936.x |