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Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles
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| Zeitschriftentitel: | Traffic |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Traffic, 7, 2006, 9, S. 1163-1176 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Rizzoli, Silvio O. Bethani, Ioanna Zwilling, Daniel Wenzel, Dirk Siddiqui, Tabrez J. Brandhorst, Dorothea Jahn, Reinhard Rizzoli, Silvio O. Bethani, Ioanna Zwilling, Daniel Wenzel, Dirk Siddiqui, Tabrez J. Brandhorst, Dorothea Jahn, Reinhard |
|---|---|
| author |
Rizzoli, Silvio O. Bethani, Ioanna Zwilling, Daniel Wenzel, Dirk Siddiqui, Tabrez J. Brandhorst, Dorothea Jahn, Reinhard |
| spellingShingle |
Rizzoli, Silvio O. Bethani, Ioanna Zwilling, Daniel Wenzel, Dirk Siddiqui, Tabrez J. Brandhorst, Dorothea Jahn, Reinhard Traffic Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles Cell Biology Genetics Molecular Biology Biochemistry Structural Biology |
| author_sort |
rizzoli, silvio o. |
| spelling |
Rizzoli, Silvio O. Bethani, Ioanna Zwilling, Daniel Wenzel, Dirk Siddiqui, Tabrez J. Brandhorst, Dorothea Jahn, Reinhard 1398-9219 1600-0854 Wiley Cell Biology Genetics Molecular Biology Biochemistry Structural Biology http://dx.doi.org/10.1111/j.1600-0854.2006.00466.x <jats:p><jats:bold>Early endosomes are well‐established acceptor compartments of endocytic vesicles in many cell types. Little evidence of their existence or function has been obtained in synapses, and it is generally believed that synaptic vesicles recycle without passing through an endosomal intermediate. We show here that the early endosomal SNARE proteins are enriched in synaptic vesicles. To investigate their function in the synapse, we isolated synaptic nerve terminals (synaptosomes), stimulated them in presence of different fluorescent markers to label the recycling vesicles and used these vesicles in <jats:italic>in vitro</jats:italic> fusion assays. The recently endocytosed vesicles underwent homotypic fusion. They also fused with endosomes from PC12 and BHK cells. The fusion process was dependent upon NSF activity. Moreover, fusion was dependent upon the early endosomal SNAREs but not upon the SNAREs involved in exocytosis. Our results thus show that at least a fraction of the vesicles endocytosed during synaptic activity are capable of fusing with early endosomes and lend support to an involvement of endosomal intermediates during recycling of synaptic vesicles.</jats:bold></jats:p> Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles Traffic |
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10.1111/j.1600-0854.2006.00466.x |
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| title |
Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles |
| title_unstemmed |
Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles |
| title_full |
Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles |
| title_fullStr |
Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles |
| title_full_unstemmed |
Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles |
| title_short |
Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles |
| title_sort |
evidence for early endosome‐like fusion of recently endocytosed synaptic vesicles |
| topic |
Cell Biology Genetics Molecular Biology Biochemistry Structural Biology |
| url |
http://dx.doi.org/10.1111/j.1600-0854.2006.00466.x |
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2006 |
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1163-1176 |
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<jats:p><jats:bold>Early endosomes are well‐established acceptor compartments of endocytic vesicles in many cell types. Little evidence of their existence or function has been obtained in synapses, and it is generally believed that synaptic vesicles recycle without passing through an endosomal intermediate. We show here that the early endosomal SNARE proteins are enriched in synaptic vesicles. To investigate their function in the synapse, we isolated synaptic nerve terminals (synaptosomes), stimulated them in presence of different fluorescent markers to label the recycling vesicles and used these vesicles in <jats:italic>in vitro</jats:italic> fusion assays. The recently endocytosed vesicles underwent homotypic fusion. They also fused with endosomes from PC12 and BHK cells. The fusion process was dependent upon NSF activity. Moreover, fusion was dependent upon the early endosomal SNAREs but not upon the SNAREs involved in exocytosis. Our results thus show that at least a fraction of the vesicles endocytosed during synaptic activity are capable of fusing with early endosomes and lend support to an involvement of endosomal intermediates during recycling of synaptic vesicles.</jats:bold></jats:p> |
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| author | Rizzoli, Silvio O., Bethani, Ioanna, Zwilling, Daniel, Wenzel, Dirk, Siddiqui, Tabrez J., Brandhorst, Dorothea, Jahn, Reinhard |
| author_facet | Rizzoli, Silvio O., Bethani, Ioanna, Zwilling, Daniel, Wenzel, Dirk, Siddiqui, Tabrez J., Brandhorst, Dorothea, Jahn, Reinhard, Rizzoli, Silvio O., Bethani, Ioanna, Zwilling, Daniel, Wenzel, Dirk, Siddiqui, Tabrez J., Brandhorst, Dorothea, Jahn, Reinhard |
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| description | <jats:p><jats:bold>Early endosomes are well‐established acceptor compartments of endocytic vesicles in many cell types. Little evidence of their existence or function has been obtained in synapses, and it is generally believed that synaptic vesicles recycle without passing through an endosomal intermediate. We show here that the early endosomal SNARE proteins are enriched in synaptic vesicles. To investigate their function in the synapse, we isolated synaptic nerve terminals (synaptosomes), stimulated them in presence of different fluorescent markers to label the recycling vesicles and used these vesicles in <jats:italic>in vitro</jats:italic> fusion assays. The recently endocytosed vesicles underwent homotypic fusion. They also fused with endosomes from PC12 and BHK cells. The fusion process was dependent upon NSF activity. Moreover, fusion was dependent upon the early endosomal SNAREs but not upon the SNAREs involved in exocytosis. Our results thus show that at least a fraction of the vesicles endocytosed during synaptic activity are capable of fusing with early endosomes and lend support to an involvement of endosomal intermediates during recycling of synaptic vesicles.</jats:bold></jats:p> |
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| spelling | Rizzoli, Silvio O. Bethani, Ioanna Zwilling, Daniel Wenzel, Dirk Siddiqui, Tabrez J. Brandhorst, Dorothea Jahn, Reinhard 1398-9219 1600-0854 Wiley Cell Biology Genetics Molecular Biology Biochemistry Structural Biology http://dx.doi.org/10.1111/j.1600-0854.2006.00466.x <jats:p><jats:bold>Early endosomes are well‐established acceptor compartments of endocytic vesicles in many cell types. Little evidence of their existence or function has been obtained in synapses, and it is generally believed that synaptic vesicles recycle without passing through an endosomal intermediate. We show here that the early endosomal SNARE proteins are enriched in synaptic vesicles. To investigate their function in the synapse, we isolated synaptic nerve terminals (synaptosomes), stimulated them in presence of different fluorescent markers to label the recycling vesicles and used these vesicles in <jats:italic>in vitro</jats:italic> fusion assays. The recently endocytosed vesicles underwent homotypic fusion. They also fused with endosomes from PC12 and BHK cells. The fusion process was dependent upon NSF activity. Moreover, fusion was dependent upon the early endosomal SNAREs but not upon the SNAREs involved in exocytosis. Our results thus show that at least a fraction of the vesicles endocytosed during synaptic activity are capable of fusing with early endosomes and lend support to an involvement of endosomal intermediates during recycling of synaptic vesicles.</jats:bold></jats:p> Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles Traffic |
| spellingShingle | Rizzoli, Silvio O., Bethani, Ioanna, Zwilling, Daniel, Wenzel, Dirk, Siddiqui, Tabrez J., Brandhorst, Dorothea, Jahn, Reinhard, Traffic, Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles, Cell Biology, Genetics, Molecular Biology, Biochemistry, Structural Biology |
| title | Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles |
| title_full | Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles |
| title_fullStr | Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles |
| title_full_unstemmed | Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles |
| title_short | Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles |
| title_sort | evidence for early endosome‐like fusion of recently endocytosed synaptic vesicles |
| title_unstemmed | Evidence for Early Endosome‐like Fusion of Recently Endocytosed Synaptic Vesicles |
| topic | Cell Biology, Genetics, Molecular Biology, Biochemistry, Structural Biology |
| url | http://dx.doi.org/10.1111/j.1600-0854.2006.00466.x |