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Artificial membranes as models in penetration investigations
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| Zeitschriftentitel: | Skin Research and Technology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Skin Research and Technology, 19, 2013, 1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Krulikowska, M. Arct, J. Lucova, M. Cetner, B. Majewski, S. Krulikowska, M. Arct, J. Lucova, M. Cetner, B. Majewski, S. |
|---|---|
| author |
Krulikowska, M. Arct, J. Lucova, M. Cetner, B. Majewski, S. |
| spellingShingle |
Krulikowska, M. Arct, J. Lucova, M. Cetner, B. Majewski, S. Skin Research and Technology Artificial membranes as models in penetration investigations Dermatology |
| author_sort |
krulikowska, m. |
| spelling |
Krulikowska, M. Arct, J. Lucova, M. Cetner, B. Majewski, S. 0909-752X 1600-0846 Wiley Dermatology http://dx.doi.org/10.1111/j.1600-0846.2012.00620.x <jats:sec><jats:title>Background/aims</jats:title><jats:p><jats:italic>In vitro</jats:italic> methods used in the research of transepidermal transport of active substances generally rely on the penetration rate of test compounds through standard membranes. Models typically used in penetration experiments are specially prepared human or animal skin samples or synthetic membranes.</jats:p><jats:p>The objective of this study was to establish if the test results for an artificial liposome membrane can be extrapolated to determine the actual bioavailability of active substances.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Tests were conducted in a side‐by‐side diffusion cell. As model membranes, a liquid‐crystal lipid membrane (<jats:styled-content style="fixed-case">LM</jats:styled-content>), phospholipid membrane (<jats:styled-content style="fixed-case">PM</jats:styled-content>) and pig skin sample were used. The test compounds were eight synthetic dyes used in hair colouring products.</jats:p></jats:sec><jats:sec><jats:title>Results/Conclusion</jats:title><jats:p>Research findings reveal that membranes composed of lipids, identical to those present in the epidermis and forming analogical liquid‐crystal structures provide a close approximation of the actual bioavailability of active substances (correlation between the results obtained for pig skin and <jats:styled-content style="fixed-case">LM</jats:styled-content> was significant: R = 0.95 and R = 0.93 in the presence of a 1% Sodium dodecyl sulphate in donor system). Unlike biological membranes, intercellular cement does not contain phospholipids. The observed correlation between penetration coefficients through the <jats:styled-content style="fixed-case">PM</jats:styled-content> and pig skin was not significant (R = 0.82). The experiments confirm that the <jats:styled-content style="fixed-case">PM</jats:styled-content> constitutes a less credible model for the studies of transepidermal transport in real life conditions.</jats:p></jats:sec> Artificial membranes as models in penetration investigations Skin Research and Technology |
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10.1111/j.1600-0846.2012.00620.x |
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Online |
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Wiley, 2013 |
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Wiley, 2013 |
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0909-752X 1600-0846 |
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0909-752X 1600-0846 |
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Artificial membranes as models in penetration investigations |
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Artificial membranes as models in penetration investigations |
| title_full |
Artificial membranes as models in penetration investigations |
| title_fullStr |
Artificial membranes as models in penetration investigations |
| title_full_unstemmed |
Artificial membranes as models in penetration investigations |
| title_short |
Artificial membranes as models in penetration investigations |
| title_sort |
artificial membranes as models in penetration investigations |
| topic |
Dermatology |
| url |
http://dx.doi.org/10.1111/j.1600-0846.2012.00620.x |
| publishDate |
2013 |
| physical |
|
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<jats:sec><jats:title>Background/aims</jats:title><jats:p><jats:italic>In vitro</jats:italic> methods used in the research of transepidermal transport of active substances generally rely on the penetration rate of test compounds through standard membranes. Models typically used in penetration experiments are specially prepared human or animal skin samples or synthetic membranes.</jats:p><jats:p>The objective of this study was to establish if the test results for an artificial liposome membrane can be extrapolated to determine the actual bioavailability of active substances.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Tests were conducted in a side‐by‐side diffusion cell. As model membranes, a liquid‐crystal lipid membrane (<jats:styled-content style="fixed-case">LM</jats:styled-content>), phospholipid membrane (<jats:styled-content style="fixed-case">PM</jats:styled-content>) and pig skin sample were used. The test compounds were eight synthetic dyes used in hair colouring products.</jats:p></jats:sec><jats:sec><jats:title>Results/Conclusion</jats:title><jats:p>Research findings reveal that membranes composed of lipids, identical to those present in the epidermis and forming analogical liquid‐crystal structures provide a close approximation of the actual bioavailability of active substances (correlation between the results obtained for pig skin and <jats:styled-content style="fixed-case">LM</jats:styled-content> was significant: R = 0.95 and R = 0.93 in the presence of a 1% Sodium dodecyl sulphate in donor system). Unlike biological membranes, intercellular cement does not contain phospholipids. The observed correlation between penetration coefficients through the <jats:styled-content style="fixed-case">PM</jats:styled-content> and pig skin was not significant (R = 0.82). The experiments confirm that the <jats:styled-content style="fixed-case">PM</jats:styled-content> constitutes a less credible model for the studies of transepidermal transport in real life conditions.</jats:p></jats:sec> |
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| author | Krulikowska, M., Arct, J., Lucova, M., Cetner, B., Majewski, S. |
| author_facet | Krulikowska, M., Arct, J., Lucova, M., Cetner, B., Majewski, S., Krulikowska, M., Arct, J., Lucova, M., Cetner, B., Majewski, S. |
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| description | <jats:sec><jats:title>Background/aims</jats:title><jats:p><jats:italic>In vitro</jats:italic> methods used in the research of transepidermal transport of active substances generally rely on the penetration rate of test compounds through standard membranes. Models typically used in penetration experiments are specially prepared human or animal skin samples or synthetic membranes.</jats:p><jats:p>The objective of this study was to establish if the test results for an artificial liposome membrane can be extrapolated to determine the actual bioavailability of active substances.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Tests were conducted in a side‐by‐side diffusion cell. As model membranes, a liquid‐crystal lipid membrane (<jats:styled-content style="fixed-case">LM</jats:styled-content>), phospholipid membrane (<jats:styled-content style="fixed-case">PM</jats:styled-content>) and pig skin sample were used. The test compounds were eight synthetic dyes used in hair colouring products.</jats:p></jats:sec><jats:sec><jats:title>Results/Conclusion</jats:title><jats:p>Research findings reveal that membranes composed of lipids, identical to those present in the epidermis and forming analogical liquid‐crystal structures provide a close approximation of the actual bioavailability of active substances (correlation between the results obtained for pig skin and <jats:styled-content style="fixed-case">LM</jats:styled-content> was significant: R = 0.95 and R = 0.93 in the presence of a 1% Sodium dodecyl sulphate in donor system). Unlike biological membranes, intercellular cement does not contain phospholipids. The observed correlation between penetration coefficients through the <jats:styled-content style="fixed-case">PM</jats:styled-content> and pig skin was not significant (R = 0.82). The experiments confirm that the <jats:styled-content style="fixed-case">PM</jats:styled-content> constitutes a less credible model for the studies of transepidermal transport in real life conditions.</jats:p></jats:sec> |
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| imprint_str_mv | Wiley, 2013 |
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| spelling | Krulikowska, M. Arct, J. Lucova, M. Cetner, B. Majewski, S. 0909-752X 1600-0846 Wiley Dermatology http://dx.doi.org/10.1111/j.1600-0846.2012.00620.x <jats:sec><jats:title>Background/aims</jats:title><jats:p><jats:italic>In vitro</jats:italic> methods used in the research of transepidermal transport of active substances generally rely on the penetration rate of test compounds through standard membranes. Models typically used in penetration experiments are specially prepared human or animal skin samples or synthetic membranes.</jats:p><jats:p>The objective of this study was to establish if the test results for an artificial liposome membrane can be extrapolated to determine the actual bioavailability of active substances.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Tests were conducted in a side‐by‐side diffusion cell. As model membranes, a liquid‐crystal lipid membrane (<jats:styled-content style="fixed-case">LM</jats:styled-content>), phospholipid membrane (<jats:styled-content style="fixed-case">PM</jats:styled-content>) and pig skin sample were used. The test compounds were eight synthetic dyes used in hair colouring products.</jats:p></jats:sec><jats:sec><jats:title>Results/Conclusion</jats:title><jats:p>Research findings reveal that membranes composed of lipids, identical to those present in the epidermis and forming analogical liquid‐crystal structures provide a close approximation of the actual bioavailability of active substances (correlation between the results obtained for pig skin and <jats:styled-content style="fixed-case">LM</jats:styled-content> was significant: R = 0.95 and R = 0.93 in the presence of a 1% Sodium dodecyl sulphate in donor system). Unlike biological membranes, intercellular cement does not contain phospholipids. The observed correlation between penetration coefficients through the <jats:styled-content style="fixed-case">PM</jats:styled-content> and pig skin was not significant (R = 0.82). The experiments confirm that the <jats:styled-content style="fixed-case">PM</jats:styled-content> constitutes a less credible model for the studies of transepidermal transport in real life conditions.</jats:p></jats:sec> Artificial membranes as models in penetration investigations Skin Research and Technology |
| spellingShingle | Krulikowska, M., Arct, J., Lucova, M., Cetner, B., Majewski, S., Skin Research and Technology, Artificial membranes as models in penetration investigations, Dermatology |
| title | Artificial membranes as models in penetration investigations |
| title_full | Artificial membranes as models in penetration investigations |
| title_fullStr | Artificial membranes as models in penetration investigations |
| title_full_unstemmed | Artificial membranes as models in penetration investigations |
| title_short | Artificial membranes as models in penetration investigations |
| title_sort | artificial membranes as models in penetration investigations |
| title_unstemmed | Artificial membranes as models in penetration investigations |
| topic | Dermatology |
| url | http://dx.doi.org/10.1111/j.1600-0846.2012.00620.x |