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Quantitative assessment of epidermal Langerhans cells using automated image analysis
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| Zeitschriftentitel: | Skin Research and Technology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Skin Research and Technology, 4, 1998, 1, S. 37-40 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Smolle, Josef Hofmann‐Wellenhof, Rainer Smolle, Josef Hofmann‐Wellenhof, Rainer |
|---|---|
| author |
Smolle, Josef Hofmann‐Wellenhof, Rainer |
| spellingShingle |
Smolle, Josef Hofmann‐Wellenhof, Rainer Skin Research and Technology Quantitative assessment of epidermal Langerhans cells using automated image analysis Dermatology |
| author_sort |
smolle, josef |
| spelling |
Smolle, Josef Hofmann‐Wellenhof, Rainer 0909-752X 1600-0846 Wiley Dermatology http://dx.doi.org/10.1111/j.1600-0846.1998.tb00084.x <jats:p><jats:bold>Background/aims: </jats:bold> Langerhans cells play a central role In the skin immune system. Quantitative changes have been observed in a variety of dermatological conditions. This paper presents a user‐independent automated measurement procedure for Langerhans cells in vertical skin sections.</jats:p><jats:p><jats:bold>Methods: </jats:bold> Frozen sections were stained immunohistochemically with a CD1 monoclonal antibody. Counterstaining was performed with Mayer's hematoxylin. An image analysis procedure was developed, which automatically recognizes the area occupied by the epidermis and the CD1 ‐positive structures, respectively.</jats:p><jats:p><jats:bold>Results: </jats:bold> The procedure was tested on specimens of normal skin and of chronic cutaneous lupus erythematosus. The results significantly reflect the decrease of Langerhans cells in the latter condition.</jats:p><jats:p><jats:bold>Conclusion: </jats:bold> The proposed image analysis program facilitates a fully automated measurement of immunohistochemically stained epidermal structures in vertical skin sections.</jats:p> Quantitative assessment of epidermal Langerhans cells using automated image analysis Skin Research and Technology |
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10.1111/j.1600-0846.1998.tb00084.x |
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1998 |
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| title |
Quantitative assessment of epidermal Langerhans cells using automated image analysis |
| title_unstemmed |
Quantitative assessment of epidermal Langerhans cells using automated image analysis |
| title_full |
Quantitative assessment of epidermal Langerhans cells using automated image analysis |
| title_fullStr |
Quantitative assessment of epidermal Langerhans cells using automated image analysis |
| title_full_unstemmed |
Quantitative assessment of epidermal Langerhans cells using automated image analysis |
| title_short |
Quantitative assessment of epidermal Langerhans cells using automated image analysis |
| title_sort |
quantitative assessment of epidermal langerhans cells using automated image analysis |
| topic |
Dermatology |
| url |
http://dx.doi.org/10.1111/j.1600-0846.1998.tb00084.x |
| publishDate |
1998 |
| physical |
37-40 |
| description |
<jats:p><jats:bold>Background/aims: </jats:bold> Langerhans cells play a central role In the skin immune system. Quantitative changes have been observed in a variety of dermatological conditions. This paper presents a user‐independent automated measurement procedure for Langerhans cells in vertical skin sections.</jats:p><jats:p><jats:bold>Methods: </jats:bold> Frozen sections were stained immunohistochemically with a CD1 monoclonal antibody. Counterstaining was performed with Mayer's hematoxylin. An image analysis procedure was developed, which automatically recognizes the area occupied by the epidermis and the CD1 ‐positive structures, respectively.</jats:p><jats:p><jats:bold>Results: </jats:bold> The procedure was tested on specimens of normal skin and of chronic cutaneous lupus erythematosus. The results significantly reflect the decrease of Langerhans cells in the latter condition.</jats:p><jats:p><jats:bold>Conclusion: </jats:bold> The proposed image analysis program facilitates a fully automated measurement of immunohistochemically stained epidermal structures in vertical skin sections.</jats:p> |
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| author | Smolle, Josef, Hofmann‐Wellenhof, Rainer |
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| author_sort | smolle, josef |
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| container_title | Skin Research and Technology |
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| description | <jats:p><jats:bold>Background/aims: </jats:bold> Langerhans cells play a central role In the skin immune system. Quantitative changes have been observed in a variety of dermatological conditions. This paper presents a user‐independent automated measurement procedure for Langerhans cells in vertical skin sections.</jats:p><jats:p><jats:bold>Methods: </jats:bold> Frozen sections were stained immunohistochemically with a CD1 monoclonal antibody. Counterstaining was performed with Mayer's hematoxylin. An image analysis procedure was developed, which automatically recognizes the area occupied by the epidermis and the CD1 ‐positive structures, respectively.</jats:p><jats:p><jats:bold>Results: </jats:bold> The procedure was tested on specimens of normal skin and of chronic cutaneous lupus erythematosus. The results significantly reflect the decrease of Langerhans cells in the latter condition.</jats:p><jats:p><jats:bold>Conclusion: </jats:bold> The proposed image analysis program facilitates a fully automated measurement of immunohistochemically stained epidermal structures in vertical skin sections.</jats:p> |
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| imprint | Wiley, 1998 |
| imprint_str_mv | Wiley, 1998 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14 |
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| physical | 37-40 |
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| publishDateSort | 1998 |
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| series | Skin Research and Technology |
| source_id | 49 |
| spelling | Smolle, Josef Hofmann‐Wellenhof, Rainer 0909-752X 1600-0846 Wiley Dermatology http://dx.doi.org/10.1111/j.1600-0846.1998.tb00084.x <jats:p><jats:bold>Background/aims: </jats:bold> Langerhans cells play a central role In the skin immune system. Quantitative changes have been observed in a variety of dermatological conditions. This paper presents a user‐independent automated measurement procedure for Langerhans cells in vertical skin sections.</jats:p><jats:p><jats:bold>Methods: </jats:bold> Frozen sections were stained immunohistochemically with a CD1 monoclonal antibody. Counterstaining was performed with Mayer's hematoxylin. An image analysis procedure was developed, which automatically recognizes the area occupied by the epidermis and the CD1 ‐positive structures, respectively.</jats:p><jats:p><jats:bold>Results: </jats:bold> The procedure was tested on specimens of normal skin and of chronic cutaneous lupus erythematosus. The results significantly reflect the decrease of Langerhans cells in the latter condition.</jats:p><jats:p><jats:bold>Conclusion: </jats:bold> The proposed image analysis program facilitates a fully automated measurement of immunohistochemically stained epidermal structures in vertical skin sections.</jats:p> Quantitative assessment of epidermal Langerhans cells using automated image analysis Skin Research and Technology |
| spellingShingle | Smolle, Josef, Hofmann‐Wellenhof, Rainer, Skin Research and Technology, Quantitative assessment of epidermal Langerhans cells using automated image analysis, Dermatology |
| title | Quantitative assessment of epidermal Langerhans cells using automated image analysis |
| title_full | Quantitative assessment of epidermal Langerhans cells using automated image analysis |
| title_fullStr | Quantitative assessment of epidermal Langerhans cells using automated image analysis |
| title_full_unstemmed | Quantitative assessment of epidermal Langerhans cells using automated image analysis |
| title_short | Quantitative assessment of epidermal Langerhans cells using automated image analysis |
| title_sort | quantitative assessment of epidermal langerhans cells using automated image analysis |
| title_unstemmed | Quantitative assessment of epidermal Langerhans cells using automated image analysis |
| topic | Dermatology |
| url | http://dx.doi.org/10.1111/j.1600-0846.1998.tb00084.x |