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TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line.
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| Zeitschriftentitel: | Acta Ophthalmologica Scandinavica |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Acta Ophthalmologica Scandinavica, 85, 2007, s240, S. 0-0 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
JANSSENS, S CASPERS, L WILLERMAIN, F DELPORTE, C JANSSENS, S CASPERS, L WILLERMAIN, F DELPORTE, C |
|---|---|
| author |
JANSSENS, S CASPERS, L WILLERMAIN, F DELPORTE, C |
| spellingShingle |
JANSSENS, S CASPERS, L WILLERMAIN, F DELPORTE, C Acta Ophthalmologica Scandinavica TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. Ophthalmology |
| author_sort |
janssens, s |
| spelling |
JANSSENS, S CASPERS, L WILLERMAIN, F DELPORTE, C 1395-3907 1600-0420 Wiley Ophthalmology http://dx.doi.org/10.1111/j.1600-0420.2007.01063_3298.x <jats:title>Abstract</jats:title><jats:p> <jats:italic>Purpose:</jats:italic> Under physiological conditions, the outer retina is dehydrated by water movements through retinal pigmented epithelial (RPE) cells. However, during intraocular inflammation, water accumulation into the retina leads to the formation of retinal oedema. Accordingly, the knowledge of the type of aquaporins (AQPs) expressed by RPE cells and its regulation by cytokines is of crucial importance. Therefore, we studied the expression of AQP4 on the retinal epithelial ARPE‐19 cell line and its regulation by cytokines.</jats:p><jats:p> <jats:italic>Methods:</jats:italic> ARPE‐19 cells were grown for 24h in the presence or absence of 100 ng/ml interferon gamma (IFN gamma) or 30 ng/ml tumor necrosis factor alpha (TNF alpha). AQP4 expression was evaluated by semi‐quantitative Western blot analysis and by immunofluorescence.</jats:p><jats:p> <jats:italic>Results:</jats:italic> Protein immunoblots of membranes from ARPE‐19 cells were probed with an affinity purified anti‐AQP4 antibody and an anti‐glucose transporter 1. The immunoblots revealed the presence of a ± 30 kDa band, corresponding to the expected molecular weight of AQP4 observed in kidney membranes used as positive control. Immunofluorescence revealed a strong AQP4 labeling. AQP4 protein expression after 24 h treatment of ARPE‐19 cells with 100 ng/ml IFN gamma or 30 ng/ml TNF alpha were respectively of 158 ± 23 % (mean ± S.E.M, n = 3, p<0.05) and 171 ± 10 % (mean ± S.E.M, n = 3, p<0.05) compared to untreated cells.</jats:p><jats:p> <jats:italic>Conclusions:</jats:italic> ARPE‐19 cells constitutively express AQP4. In addition, AQP4 expression was significantly increased in response to IFN gamma and TNF alpha.</jats:p> TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. Acta Ophthalmologica Scandinavica |
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10.1111/j.1600-0420.2007.01063_3298.x |
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| title |
TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. |
| title_unstemmed |
TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. |
| title_full |
TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. |
| title_fullStr |
TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. |
| title_full_unstemmed |
TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. |
| title_short |
TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. |
| title_sort |
tnf‐alpha and interferon‐gamma upregulate aqp4 protein expression in arpe‐19, a human retinal pigmented epithelial cell line. |
| topic |
Ophthalmology |
| url |
http://dx.doi.org/10.1111/j.1600-0420.2007.01063_3298.x |
| publishDate |
2007 |
| physical |
0-0 |
| description |
<jats:title>Abstract</jats:title><jats:p> <jats:italic>Purpose:</jats:italic> Under physiological conditions, the outer retina is dehydrated by water movements through retinal pigmented epithelial (RPE) cells. However, during intraocular inflammation, water accumulation into the retina leads to the formation of retinal oedema. Accordingly, the knowledge of the type of aquaporins (AQPs) expressed by RPE cells and its regulation by cytokines is of crucial importance. Therefore, we studied the expression of AQP4 on the retinal epithelial ARPE‐19 cell line and its regulation by cytokines.</jats:p><jats:p> <jats:italic>Methods:</jats:italic> ARPE‐19 cells were grown for 24h in the presence or absence of 100 ng/ml interferon gamma (IFN gamma) or 30 ng/ml tumor necrosis factor alpha (TNF alpha). AQP4 expression was evaluated by semi‐quantitative Western blot analysis and by immunofluorescence.</jats:p><jats:p> <jats:italic>Results:</jats:italic> Protein immunoblots of membranes from ARPE‐19 cells were probed with an affinity purified anti‐AQP4 antibody and an anti‐glucose transporter 1. The immunoblots revealed the presence of a ± 30 kDa band, corresponding to the expected molecular weight of AQP4 observed in kidney membranes used as positive control. Immunofluorescence revealed a strong AQP4 labeling. AQP4 protein expression after 24 h treatment of ARPE‐19 cells with 100 ng/ml IFN gamma or 30 ng/ml TNF alpha were respectively of 158 ± 23 % (mean ± S.E.M, n = 3, p<0.05) and 171 ± 10 % (mean ± S.E.M, n = 3, p<0.05) compared to untreated cells.</jats:p><jats:p> <jats:italic>Conclusions:</jats:italic> ARPE‐19 cells constitutively express AQP4. In addition, AQP4 expression was significantly increased in response to IFN gamma and TNF alpha.</jats:p> |
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| author | JANSSENS, S, CASPERS, L, WILLERMAIN, F, DELPORTE, C |
| author_facet | JANSSENS, S, CASPERS, L, WILLERMAIN, F, DELPORTE, C, JANSSENS, S, CASPERS, L, WILLERMAIN, F, DELPORTE, C |
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| description | <jats:title>Abstract</jats:title><jats:p> <jats:italic>Purpose:</jats:italic> Under physiological conditions, the outer retina is dehydrated by water movements through retinal pigmented epithelial (RPE) cells. However, during intraocular inflammation, water accumulation into the retina leads to the formation of retinal oedema. Accordingly, the knowledge of the type of aquaporins (AQPs) expressed by RPE cells and its regulation by cytokines is of crucial importance. Therefore, we studied the expression of AQP4 on the retinal epithelial ARPE‐19 cell line and its regulation by cytokines.</jats:p><jats:p> <jats:italic>Methods:</jats:italic> ARPE‐19 cells were grown for 24h in the presence or absence of 100 ng/ml interferon gamma (IFN gamma) or 30 ng/ml tumor necrosis factor alpha (TNF alpha). AQP4 expression was evaluated by semi‐quantitative Western blot analysis and by immunofluorescence.</jats:p><jats:p> <jats:italic>Results:</jats:italic> Protein immunoblots of membranes from ARPE‐19 cells were probed with an affinity purified anti‐AQP4 antibody and an anti‐glucose transporter 1. The immunoblots revealed the presence of a ± 30 kDa band, corresponding to the expected molecular weight of AQP4 observed in kidney membranes used as positive control. Immunofluorescence revealed a strong AQP4 labeling. AQP4 protein expression after 24 h treatment of ARPE‐19 cells with 100 ng/ml IFN gamma or 30 ng/ml TNF alpha were respectively of 158 ± 23 % (mean ± S.E.M, n = 3, p<0.05) and 171 ± 10 % (mean ± S.E.M, n = 3, p<0.05) compared to untreated cells.</jats:p><jats:p> <jats:italic>Conclusions:</jats:italic> ARPE‐19 cells constitutively express AQP4. In addition, AQP4 expression was significantly increased in response to IFN gamma and TNF alpha.</jats:p> |
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| imprint_str_mv | Wiley, 2007 |
| institution | DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15 |
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| spelling | JANSSENS, S CASPERS, L WILLERMAIN, F DELPORTE, C 1395-3907 1600-0420 Wiley Ophthalmology http://dx.doi.org/10.1111/j.1600-0420.2007.01063_3298.x <jats:title>Abstract</jats:title><jats:p> <jats:italic>Purpose:</jats:italic> Under physiological conditions, the outer retina is dehydrated by water movements through retinal pigmented epithelial (RPE) cells. However, during intraocular inflammation, water accumulation into the retina leads to the formation of retinal oedema. Accordingly, the knowledge of the type of aquaporins (AQPs) expressed by RPE cells and its regulation by cytokines is of crucial importance. Therefore, we studied the expression of AQP4 on the retinal epithelial ARPE‐19 cell line and its regulation by cytokines.</jats:p><jats:p> <jats:italic>Methods:</jats:italic> ARPE‐19 cells were grown for 24h in the presence or absence of 100 ng/ml interferon gamma (IFN gamma) or 30 ng/ml tumor necrosis factor alpha (TNF alpha). AQP4 expression was evaluated by semi‐quantitative Western blot analysis and by immunofluorescence.</jats:p><jats:p> <jats:italic>Results:</jats:italic> Protein immunoblots of membranes from ARPE‐19 cells were probed with an affinity purified anti‐AQP4 antibody and an anti‐glucose transporter 1. The immunoblots revealed the presence of a ± 30 kDa band, corresponding to the expected molecular weight of AQP4 observed in kidney membranes used as positive control. Immunofluorescence revealed a strong AQP4 labeling. AQP4 protein expression after 24 h treatment of ARPE‐19 cells with 100 ng/ml IFN gamma or 30 ng/ml TNF alpha were respectively of 158 ± 23 % (mean ± S.E.M, n = 3, p<0.05) and 171 ± 10 % (mean ± S.E.M, n = 3, p<0.05) compared to untreated cells.</jats:p><jats:p> <jats:italic>Conclusions:</jats:italic> ARPE‐19 cells constitutively express AQP4. In addition, AQP4 expression was significantly increased in response to IFN gamma and TNF alpha.</jats:p> TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. Acta Ophthalmologica Scandinavica |
| spellingShingle | JANSSENS, S, CASPERS, L, WILLERMAIN, F, DELPORTE, C, Acta Ophthalmologica Scandinavica, TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line., Ophthalmology |
| title | TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. |
| title_full | TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. |
| title_fullStr | TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. |
| title_full_unstemmed | TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. |
| title_short | TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. |
| title_sort | tnf‐alpha and interferon‐gamma upregulate aqp4 protein expression in arpe‐19, a human retinal pigmented epithelial cell line. |
| title_unstemmed | TNF‐alpha and Interferon‐gamma upregulate AQP4 protein expression in ARPE‐19, a human retinal pigmented epithelial cell line. |
| topic | Ophthalmology |
| url | http://dx.doi.org/10.1111/j.1600-0420.2007.01063_3298.x |