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Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation
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Zeitschriftentitel: | Helicobacter |
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Personen und Körperschaften: | , |
In: | Helicobacter, 14, 2009, 2, S. 141-146 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Wiley
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author_facet |
Kwon, Dong H. Versalovic, James Kwon, Dong H. Versalovic, James |
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author |
Kwon, Dong H. Versalovic, James |
spellingShingle |
Kwon, Dong H. Versalovic, James Helicobacter Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation Infectious Diseases Gastroenterology General Medicine |
author_sort |
kwon, dong h. |
spelling |
Kwon, Dong H. Versalovic, James 1083-4389 1523-5378 Wiley Infectious Diseases Gastroenterology General Medicine http://dx.doi.org/10.1111/j.1523-5378.2009.00669.x <jats:title>Abstract</jats:title><jats:p><jats:bold>Background and Aims:</jats:bold> <jats:italic>Helicobacter pylori</jats:italic> infection is associated with a variety of diseases including gastric cancer. Flavodoxin is an electron transfer protein containing a flavin mononucleotide prosthetic group and substituted an iron‐containing electron transfer protein under iron‐limiting conditions. <jats:italic>H. pylori</jats:italic> flavodoxin has been reported but its pathogenic role is unclear. The aim of this study is to understand a pathogenic role of <jats:italic>H. pylori </jats:italic>flavodoxin under iron‐limiting condition.</jats:p><jats:p><jats:bold>Methods:</jats:bold> The flavodoxin‐encoding gene (<jats:italic>fldA</jats:italic>) was cloned from one of clinical <jats:italic>H. pylori</jats:italic> isolates (DU17) and its transcript was quantified by primer extension, Northern hybridization, and real‐time polymerase chain reaction in different concentrations of an iron chelator. The <jats:italic>fldA</jats:italic> transcript was also quantified in <jats:italic>H. pylori</jats:italic> ATCC 700392, lacking a ferric uptake regulatory (fur) protein.</jats:p><jats:p><jats:bold>Result:</jats:bold> Nucleotide sequence of the <jats:italic>fldA</jats:italic> from <jats:italic>H. pylori</jats:italic> DU17 revealed a 492‐bp (164 amino acids) open reading frame with a deduced amino acid sequence having 97% identity to that from the complete genomic sequence of <jats:italic>H. pylori </jats:italic>26695. The deduced promoter [–35, –10, and +1] of the <jats:italic>fldA</jats:italic> was 56‐bp upstream from the first codon of FldA. The <jats:italic>fldA </jats:italic>transcript (~0.55‐kb) was induced up to 14‐fold in both wild‐type and <jats:italic>fur</jats:italic>‐knocked‐out strains under iron‐limiting conditions, suggesting that the <jats:italic>fldA</jats:italic> induction is independent to the Fur protein.</jats:p><jats:p><jats:bold>Conclusion:</jats:bold> The <jats:italic>fldA</jats:italic> gene may play an important role in iron starvation conditions.</jats:p> Fur‐Independent Induction of <i>Helicobacter pylori</i> Flavodoxin‐Encoding Gene (<i>fldA</i>) Under Iron Starvation Helicobacter |
doi_str_mv |
10.1111/j.1523-5378.2009.00669.x |
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Online |
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Medizin |
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ElectronicArticle |
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ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTExMS9qLjE1MjMtNTM3OC4yMDA5LjAwNjY5Lng |
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DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 |
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Wiley, 2009 |
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Wiley, 2009 |
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1083-4389 1523-5378 |
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1083-4389 1523-5378 |
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English |
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2009 |
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Wiley |
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Helicobacter |
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49 |
title |
Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation |
title_unstemmed |
Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation |
title_full |
Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation |
title_fullStr |
Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation |
title_full_unstemmed |
Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation |
title_short |
Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation |
title_sort |
fur‐independent induction of <i>helicobacter pylori</i> flavodoxin‐encoding gene (<i>flda</i>) under iron starvation |
topic |
Infectious Diseases Gastroenterology General Medicine |
url |
http://dx.doi.org/10.1111/j.1523-5378.2009.00669.x |
publishDate |
2009 |
physical |
141-146 |
description |
<jats:title>Abstract</jats:title><jats:p><jats:bold>Background and Aims:</jats:bold> <jats:italic>Helicobacter pylori</jats:italic> infection is associated with a variety of diseases including gastric cancer. Flavodoxin is an electron transfer protein containing a flavin mononucleotide prosthetic group and substituted an iron‐containing electron transfer protein under iron‐limiting conditions. <jats:italic>H. pylori</jats:italic> flavodoxin has been reported but its pathogenic role is unclear. The aim of this study is to understand a pathogenic role of <jats:italic>H. pylori </jats:italic>flavodoxin under iron‐limiting condition.</jats:p><jats:p><jats:bold>Methods:</jats:bold> The flavodoxin‐encoding gene (<jats:italic>fldA</jats:italic>) was cloned from one of clinical <jats:italic>H. pylori</jats:italic> isolates (DU17) and its transcript was quantified by primer extension, Northern hybridization, and real‐time polymerase chain reaction in different concentrations of an iron chelator. The <jats:italic>fldA</jats:italic> transcript was also quantified in <jats:italic>H. pylori</jats:italic> ATCC 700392, lacking a ferric uptake regulatory (fur) protein.</jats:p><jats:p><jats:bold>Result:</jats:bold> Nucleotide sequence of the <jats:italic>fldA</jats:italic> from <jats:italic>H. pylori</jats:italic> DU17 revealed a 492‐bp (164 amino acids) open reading frame with a deduced amino acid sequence having 97% identity to that from the complete genomic sequence of <jats:italic>H. pylori </jats:italic>26695. The deduced promoter [–35, –10, and +1] of the <jats:italic>fldA</jats:italic> was 56‐bp upstream from the first codon of FldA. The <jats:italic>fldA </jats:italic>transcript (~0.55‐kb) was induced up to 14‐fold in both wild‐type and <jats:italic>fur</jats:italic>‐knocked‐out strains under iron‐limiting conditions, suggesting that the <jats:italic>fldA</jats:italic> induction is independent to the Fur protein.</jats:p><jats:p><jats:bold>Conclusion:</jats:bold> The <jats:italic>fldA</jats:italic> gene may play an important role in iron starvation conditions.</jats:p> |
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author | Kwon, Dong H., Versalovic, James |
author_facet | Kwon, Dong H., Versalovic, James, Kwon, Dong H., Versalovic, James |
author_sort | kwon, dong h. |
container_issue | 2 |
container_start_page | 141 |
container_title | Helicobacter |
container_volume | 14 |
description | <jats:title>Abstract</jats:title><jats:p><jats:bold>Background and Aims:</jats:bold> <jats:italic>Helicobacter pylori</jats:italic> infection is associated with a variety of diseases including gastric cancer. Flavodoxin is an electron transfer protein containing a flavin mononucleotide prosthetic group and substituted an iron‐containing electron transfer protein under iron‐limiting conditions. <jats:italic>H. pylori</jats:italic> flavodoxin has been reported but its pathogenic role is unclear. The aim of this study is to understand a pathogenic role of <jats:italic>H. pylori </jats:italic>flavodoxin under iron‐limiting condition.</jats:p><jats:p><jats:bold>Methods:</jats:bold> The flavodoxin‐encoding gene (<jats:italic>fldA</jats:italic>) was cloned from one of clinical <jats:italic>H. pylori</jats:italic> isolates (DU17) and its transcript was quantified by primer extension, Northern hybridization, and real‐time polymerase chain reaction in different concentrations of an iron chelator. The <jats:italic>fldA</jats:italic> transcript was also quantified in <jats:italic>H. pylori</jats:italic> ATCC 700392, lacking a ferric uptake regulatory (fur) protein.</jats:p><jats:p><jats:bold>Result:</jats:bold> Nucleotide sequence of the <jats:italic>fldA</jats:italic> from <jats:italic>H. pylori</jats:italic> DU17 revealed a 492‐bp (164 amino acids) open reading frame with a deduced amino acid sequence having 97% identity to that from the complete genomic sequence of <jats:italic>H. pylori </jats:italic>26695. The deduced promoter [–35, –10, and +1] of the <jats:italic>fldA</jats:italic> was 56‐bp upstream from the first codon of FldA. The <jats:italic>fldA </jats:italic>transcript (~0.55‐kb) was induced up to 14‐fold in both wild‐type and <jats:italic>fur</jats:italic>‐knocked‐out strains under iron‐limiting conditions, suggesting that the <jats:italic>fldA</jats:italic> induction is independent to the Fur protein.</jats:p><jats:p><jats:bold>Conclusion:</jats:bold> The <jats:italic>fldA</jats:italic> gene may play an important role in iron starvation conditions.</jats:p> |
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institution | DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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spelling | Kwon, Dong H. Versalovic, James 1083-4389 1523-5378 Wiley Infectious Diseases Gastroenterology General Medicine http://dx.doi.org/10.1111/j.1523-5378.2009.00669.x <jats:title>Abstract</jats:title><jats:p><jats:bold>Background and Aims:</jats:bold> <jats:italic>Helicobacter pylori</jats:italic> infection is associated with a variety of diseases including gastric cancer. Flavodoxin is an electron transfer protein containing a flavin mononucleotide prosthetic group and substituted an iron‐containing electron transfer protein under iron‐limiting conditions. <jats:italic>H. pylori</jats:italic> flavodoxin has been reported but its pathogenic role is unclear. The aim of this study is to understand a pathogenic role of <jats:italic>H. pylori </jats:italic>flavodoxin under iron‐limiting condition.</jats:p><jats:p><jats:bold>Methods:</jats:bold> The flavodoxin‐encoding gene (<jats:italic>fldA</jats:italic>) was cloned from one of clinical <jats:italic>H. pylori</jats:italic> isolates (DU17) and its transcript was quantified by primer extension, Northern hybridization, and real‐time polymerase chain reaction in different concentrations of an iron chelator. The <jats:italic>fldA</jats:italic> transcript was also quantified in <jats:italic>H. pylori</jats:italic> ATCC 700392, lacking a ferric uptake regulatory (fur) protein.</jats:p><jats:p><jats:bold>Result:</jats:bold> Nucleotide sequence of the <jats:italic>fldA</jats:italic> from <jats:italic>H. pylori</jats:italic> DU17 revealed a 492‐bp (164 amino acids) open reading frame with a deduced amino acid sequence having 97% identity to that from the complete genomic sequence of <jats:italic>H. pylori </jats:italic>26695. The deduced promoter [–35, –10, and +1] of the <jats:italic>fldA</jats:italic> was 56‐bp upstream from the first codon of FldA. The <jats:italic>fldA </jats:italic>transcript (~0.55‐kb) was induced up to 14‐fold in both wild‐type and <jats:italic>fur</jats:italic>‐knocked‐out strains under iron‐limiting conditions, suggesting that the <jats:italic>fldA</jats:italic> induction is independent to the Fur protein.</jats:p><jats:p><jats:bold>Conclusion:</jats:bold> The <jats:italic>fldA</jats:italic> gene may play an important role in iron starvation conditions.</jats:p> Fur‐Independent Induction of <i>Helicobacter pylori</i> Flavodoxin‐Encoding Gene (<i>fldA</i>) Under Iron Starvation Helicobacter |
spellingShingle | Kwon, Dong H., Versalovic, James, Helicobacter, Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation, Infectious Diseases, Gastroenterology, General Medicine |
title | Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation |
title_full | Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation |
title_fullStr | Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation |
title_full_unstemmed | Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation |
title_short | Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation |
title_sort | fur‐independent induction of <i>helicobacter pylori</i> flavodoxin‐encoding gene (<i>flda</i>) under iron starvation |
title_unstemmed | Fur‐Independent Induction of Helicobacter pylori Flavodoxin‐Encoding Gene (fldA) Under Iron Starvation |
topic | Infectious Diseases, Gastroenterology, General Medicine |
url | http://dx.doi.org/10.1111/j.1523-5378.2009.00669.x |