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The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion
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| Zeitschriftentitel: | British Journal of Pharmacology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | British Journal of Pharmacology, 79, 1983, 2, S. 363-371 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Gilfillan, A.M. Hollingsworth, M. Jones, A.W. Gilfillan, A.M. Hollingsworth, M. Jones, A.W. |
|---|---|
| author |
Gilfillan, A.M. Hollingsworth, M. Jones, A.W. |
| spellingShingle |
Gilfillan, A.M. Hollingsworth, M. Jones, A.W. British Journal of Pharmacology The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion Pharmacology |
| author_sort |
gilfillan, a.m. |
| spelling |
Gilfillan, A.M. Hollingsworth, M. Jones, A.W. 0007-1188 1476-5381 Wiley Pharmacology http://dx.doi.org/10.1111/j.1476-5381.1983.tb11008.x <jats:p><jats:list list-type="explicit-label"> <jats:list-item><jats:p>Lung slices from adult rats incubated in [methyl‐<jats:sup>3</jats:sup>H]‐choline chloride formed [<jats:sup>3</jats:sup>H]‐disaturated phosphatidylcholine ([<jats:sup>3</jats:sup>H]‐DSPC) which was used as an index of lung surfactant.</jats:p></jats:list-item> <jats:list-item><jats:p>The slices were perifused after 3 h incubation in [methyl‐<jats:sup>3</jats:sup>H]‐choline chloride and the overflow of [<jats:sup>3</jats:sup>H]‐DSPC, as a rate coefficient, was used as a measure of surfactant secretion. The basal overflow of [<jats:sup>3</jats:sup>H]‐DSPC rapidly declined over the first 30 min of perifusion and then declined slowly.</jats:p></jats:list-item> <jats:list-item><jats:p>Salbutamol induced a prolonged, and sometimes delayed, increase in [<jats:sup>3</jats:sup>H]‐DSPC overflow, which was reduced by (±)‐propranolol. Potassium chloride produced an immediate, and usually transient, increase in [<jats:sup>3</jats:sup>H]‐DSPC overflow which was not modified by atropine or (±)‐propranolol. Adenosine 5′‐triphosphate, but not phenylephrine, also increased [<jats:sup>3</jats:sup>H]‐DSPC overflow.</jats:p></jats:list-item> <jats:list-item><jats:p>This method can measure the magnitude and time‐course of lung surfactant secretion induced by drugs.</jats:p></jats:list-item> </jats:list></jats:p> The pharmacological modulation of [<sup>3</sup>H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion British Journal of Pharmacology |
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10.1111/j.1476-5381.1983.tb11008.x |
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Wiley, 1983 |
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Wiley, 1983 |
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1983 |
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British Journal of Pharmacology |
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49 |
| title |
The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| title_unstemmed |
The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| title_full |
The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| title_fullStr |
The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| title_full_unstemmed |
The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| title_short |
The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| title_sort |
the pharmacological modulation of [<sup>3</sup>h]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| topic |
Pharmacology |
| url |
http://dx.doi.org/10.1111/j.1476-5381.1983.tb11008.x |
| publishDate |
1983 |
| physical |
363-371 |
| description |
<jats:p><jats:list list-type="explicit-label">
<jats:list-item><jats:p>Lung slices from adult rats incubated in [methyl‐<jats:sup>3</jats:sup>H]‐choline chloride formed [<jats:sup>3</jats:sup>H]‐disaturated phosphatidylcholine ([<jats:sup>3</jats:sup>H]‐DSPC) which was used as an index of lung surfactant.</jats:p></jats:list-item>
<jats:list-item><jats:p>The slices were perifused after 3 h incubation in [methyl‐<jats:sup>3</jats:sup>H]‐choline chloride and the overflow of [<jats:sup>3</jats:sup>H]‐DSPC, as a rate coefficient, was used as a measure of surfactant secretion. The basal overflow of [<jats:sup>3</jats:sup>H]‐DSPC rapidly declined over the first 30 min of perifusion and then declined slowly.</jats:p></jats:list-item>
<jats:list-item><jats:p>Salbutamol induced a prolonged, and sometimes delayed, increase in [<jats:sup>3</jats:sup>H]‐DSPC overflow, which was reduced by (±)‐propranolol. Potassium chloride produced an immediate, and usually transient, increase in [<jats:sup>3</jats:sup>H]‐DSPC overflow which was not modified by atropine or (±)‐propranolol. Adenosine 5′‐triphosphate, but not phenylephrine, also increased [<jats:sup>3</jats:sup>H]‐DSPC overflow.</jats:p></jats:list-item>
<jats:list-item><jats:p>This method can measure the magnitude and time‐course of lung surfactant secretion induced by drugs.</jats:p></jats:list-item>
</jats:list></jats:p> |
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| author | Gilfillan, A.M., Hollingsworth, M., Jones, A.W. |
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| author_sort | gilfillan, a.m. |
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| description | <jats:p><jats:list list-type="explicit-label"> <jats:list-item><jats:p>Lung slices from adult rats incubated in [methyl‐<jats:sup>3</jats:sup>H]‐choline chloride formed [<jats:sup>3</jats:sup>H]‐disaturated phosphatidylcholine ([<jats:sup>3</jats:sup>H]‐DSPC) which was used as an index of lung surfactant.</jats:p></jats:list-item> <jats:list-item><jats:p>The slices were perifused after 3 h incubation in [methyl‐<jats:sup>3</jats:sup>H]‐choline chloride and the overflow of [<jats:sup>3</jats:sup>H]‐DSPC, as a rate coefficient, was used as a measure of surfactant secretion. The basal overflow of [<jats:sup>3</jats:sup>H]‐DSPC rapidly declined over the first 30 min of perifusion and then declined slowly.</jats:p></jats:list-item> <jats:list-item><jats:p>Salbutamol induced a prolonged, and sometimes delayed, increase in [<jats:sup>3</jats:sup>H]‐DSPC overflow, which was reduced by (±)‐propranolol. Potassium chloride produced an immediate, and usually transient, increase in [<jats:sup>3</jats:sup>H]‐DSPC overflow which was not modified by atropine or (±)‐propranolol. Adenosine 5′‐triphosphate, but not phenylephrine, also increased [<jats:sup>3</jats:sup>H]‐DSPC overflow.</jats:p></jats:list-item> <jats:list-item><jats:p>This method can measure the magnitude and time‐course of lung surfactant secretion induced by drugs.</jats:p></jats:list-item> </jats:list></jats:p> |
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| imprint | Wiley, 1983 |
| imprint_str_mv | Wiley, 1983 |
| institution | DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15 |
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| match_str | gilfillan1983thepharmacologicalmodulationof3hdisaturatedphosphatidylcholineoverflowfromperifusedlungslicesofadultratsanewmethodforthestudyoflungsurfactantsecretion |
| mega_collection | Wiley (CrossRef) |
| physical | 363-371 |
| publishDate | 1983 |
| publishDateSort | 1983 |
| publisher | Wiley |
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| recordtype | ai |
| series | British Journal of Pharmacology |
| source_id | 49 |
| spelling | Gilfillan, A.M. Hollingsworth, M. Jones, A.W. 0007-1188 1476-5381 Wiley Pharmacology http://dx.doi.org/10.1111/j.1476-5381.1983.tb11008.x <jats:p><jats:list list-type="explicit-label"> <jats:list-item><jats:p>Lung slices from adult rats incubated in [methyl‐<jats:sup>3</jats:sup>H]‐choline chloride formed [<jats:sup>3</jats:sup>H]‐disaturated phosphatidylcholine ([<jats:sup>3</jats:sup>H]‐DSPC) which was used as an index of lung surfactant.</jats:p></jats:list-item> <jats:list-item><jats:p>The slices were perifused after 3 h incubation in [methyl‐<jats:sup>3</jats:sup>H]‐choline chloride and the overflow of [<jats:sup>3</jats:sup>H]‐DSPC, as a rate coefficient, was used as a measure of surfactant secretion. The basal overflow of [<jats:sup>3</jats:sup>H]‐DSPC rapidly declined over the first 30 min of perifusion and then declined slowly.</jats:p></jats:list-item> <jats:list-item><jats:p>Salbutamol induced a prolonged, and sometimes delayed, increase in [<jats:sup>3</jats:sup>H]‐DSPC overflow, which was reduced by (±)‐propranolol. Potassium chloride produced an immediate, and usually transient, increase in [<jats:sup>3</jats:sup>H]‐DSPC overflow which was not modified by atropine or (±)‐propranolol. Adenosine 5′‐triphosphate, but not phenylephrine, also increased [<jats:sup>3</jats:sup>H]‐DSPC overflow.</jats:p></jats:list-item> <jats:list-item><jats:p>This method can measure the magnitude and time‐course of lung surfactant secretion induced by drugs.</jats:p></jats:list-item> </jats:list></jats:p> The pharmacological modulation of [<sup>3</sup>H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion British Journal of Pharmacology |
| spellingShingle | Gilfillan, A.M., Hollingsworth, M., Jones, A.W., British Journal of Pharmacology, The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion, Pharmacology |
| title | The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| title_full | The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| title_fullStr | The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| title_full_unstemmed | The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| title_short | The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| title_sort | the pharmacological modulation of [<sup>3</sup>h]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| title_unstemmed | The pharmacological modulation of [3H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion |
| topic | Pharmacology |
| url | http://dx.doi.org/10.1111/j.1476-5381.1983.tb11008.x |