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Engineering virus resistance using a modified potato gene
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| Zeitschriftentitel: | Plant Biotechnology Journal |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Plant Biotechnology Journal, 9, 2011, 9, S. 1014-1021 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Cavatorta, Jason Perez, Kari W. Gray, Stewart M. Van Eck, Joyce Yeam, Inhwa Jahn, Molly Cavatorta, Jason Perez, Kari W. Gray, Stewart M. Van Eck, Joyce Yeam, Inhwa Jahn, Molly |
|---|---|
| author |
Cavatorta, Jason Perez, Kari W. Gray, Stewart M. Van Eck, Joyce Yeam, Inhwa Jahn, Molly |
| spellingShingle |
Cavatorta, Jason Perez, Kari W. Gray, Stewart M. Van Eck, Joyce Yeam, Inhwa Jahn, Molly Plant Biotechnology Journal Engineering virus resistance using a modified potato gene Plant Science Agronomy and Crop Science Biotechnology |
| author_sort |
cavatorta, jason |
| spelling |
Cavatorta, Jason Perez, Kari W. Gray, Stewart M. Van Eck, Joyce Yeam, Inhwa Jahn, Molly 1467-7644 1467-7652 Wiley Plant Science Agronomy and Crop Science Biotechnology http://dx.doi.org/10.1111/j.1467-7652.2011.00622.x <jats:title>Summary</jats:title><jats:p>Natural mutations in translation initiation factor eIF4E confer resistance to potyviruses in many plant species. Potato is a staple food crop plagued by several potyviruses, yet to date no known eIF4E‐mediated resistance genes have been identified. In this study, we demonstrate that transgenic expression of the <jats:italic>pvr1</jats:italic><jats:sup>2</jats:sup> gene from pepper confers resistance to <jats:italic>Potato virus Y</jats:italic> (PVY) in potato. We then use this information to convert the susceptible potato ortholog of this allele into a <jats:italic>de novo</jats:italic> allele for resistance to PVY using site‐directed mutagenesis. Potato plants overexpressing the mutated potato allele are resistant to virus infection. Resistant lines expressed high levels of eIF4E mRNA and protein. The resistant plants showed growth similar to untransformed controls and produced phenotypically similar tubers. This technique disrupts a key step in the viral infection process and may potentially be used to engineer virus resistance in a number of economically important plant–viral pathosystems. Furthermore, the general public may be more amenable to the ‘intragenic’ nature of this approach because the transferred coding region is modified from a gene in the target crop rather than from a distant species.</jats:p> Engineering virus resistance using a modified potato gene Plant Biotechnology Journal |
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10.1111/j.1467-7652.2011.00622.x |
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Biologie Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft Technik |
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Wiley, 2011 |
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| title |
Engineering virus resistance using a modified potato gene |
| title_unstemmed |
Engineering virus resistance using a modified potato gene |
| title_full |
Engineering virus resistance using a modified potato gene |
| title_fullStr |
Engineering virus resistance using a modified potato gene |
| title_full_unstemmed |
Engineering virus resistance using a modified potato gene |
| title_short |
Engineering virus resistance using a modified potato gene |
| title_sort |
engineering virus resistance using a modified potato gene |
| topic |
Plant Science Agronomy and Crop Science Biotechnology |
| url |
http://dx.doi.org/10.1111/j.1467-7652.2011.00622.x |
| publishDate |
2011 |
| physical |
1014-1021 |
| description |
<jats:title>Summary</jats:title><jats:p>Natural mutations in translation initiation factor eIF4E confer resistance to potyviruses in many plant species. Potato is a staple food crop plagued by several potyviruses, yet to date no known eIF4E‐mediated resistance genes have been identified. In this study, we demonstrate that transgenic expression of the <jats:italic>pvr1</jats:italic><jats:sup>2</jats:sup> gene from pepper confers resistance to <jats:italic>Potato virus Y</jats:italic> (PVY) in potato. We then use this information to convert the susceptible potato ortholog of this allele into a <jats:italic>de novo</jats:italic> allele for resistance to PVY using site‐directed mutagenesis. Potato plants overexpressing the mutated potato allele are resistant to virus infection. Resistant lines expressed high levels of eIF4E mRNA and protein. The resistant plants showed growth similar to untransformed controls and produced phenotypically similar tubers. This technique disrupts a key step in the viral infection process and may potentially be used to engineer virus resistance in a number of economically important plant–viral pathosystems. Furthermore, the general public may be more amenable to the ‘intragenic’ nature of this approach because the transferred coding region is modified from a gene in the target crop rather than from a distant species.</jats:p> |
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| author | Cavatorta, Jason, Perez, Kari W., Gray, Stewart M., Van Eck, Joyce, Yeam, Inhwa, Jahn, Molly |
| author_facet | Cavatorta, Jason, Perez, Kari W., Gray, Stewart M., Van Eck, Joyce, Yeam, Inhwa, Jahn, Molly, Cavatorta, Jason, Perez, Kari W., Gray, Stewart M., Van Eck, Joyce, Yeam, Inhwa, Jahn, Molly |
| author_sort | cavatorta, jason |
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| description | <jats:title>Summary</jats:title><jats:p>Natural mutations in translation initiation factor eIF4E confer resistance to potyviruses in many plant species. Potato is a staple food crop plagued by several potyviruses, yet to date no known eIF4E‐mediated resistance genes have been identified. In this study, we demonstrate that transgenic expression of the <jats:italic>pvr1</jats:italic><jats:sup>2</jats:sup> gene from pepper confers resistance to <jats:italic>Potato virus Y</jats:italic> (PVY) in potato. We then use this information to convert the susceptible potato ortholog of this allele into a <jats:italic>de novo</jats:italic> allele for resistance to PVY using site‐directed mutagenesis. Potato plants overexpressing the mutated potato allele are resistant to virus infection. Resistant lines expressed high levels of eIF4E mRNA and protein. The resistant plants showed growth similar to untransformed controls and produced phenotypically similar tubers. This technique disrupts a key step in the viral infection process and may potentially be used to engineer virus resistance in a number of economically important plant–viral pathosystems. Furthermore, the general public may be more amenable to the ‘intragenic’ nature of this approach because the transferred coding region is modified from a gene in the target crop rather than from a distant species.</jats:p> |
| doi_str_mv | 10.1111/j.1467-7652.2011.00622.x |
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| imprint_str_mv | Wiley, 2011 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
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| physical | 1014-1021 |
| publishDate | 2011 |
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| series | Plant Biotechnology Journal |
| source_id | 49 |
| spelling | Cavatorta, Jason Perez, Kari W. Gray, Stewart M. Van Eck, Joyce Yeam, Inhwa Jahn, Molly 1467-7644 1467-7652 Wiley Plant Science Agronomy and Crop Science Biotechnology http://dx.doi.org/10.1111/j.1467-7652.2011.00622.x <jats:title>Summary</jats:title><jats:p>Natural mutations in translation initiation factor eIF4E confer resistance to potyviruses in many plant species. Potato is a staple food crop plagued by several potyviruses, yet to date no known eIF4E‐mediated resistance genes have been identified. In this study, we demonstrate that transgenic expression of the <jats:italic>pvr1</jats:italic><jats:sup>2</jats:sup> gene from pepper confers resistance to <jats:italic>Potato virus Y</jats:italic> (PVY) in potato. We then use this information to convert the susceptible potato ortholog of this allele into a <jats:italic>de novo</jats:italic> allele for resistance to PVY using site‐directed mutagenesis. Potato plants overexpressing the mutated potato allele are resistant to virus infection. Resistant lines expressed high levels of eIF4E mRNA and protein. The resistant plants showed growth similar to untransformed controls and produced phenotypically similar tubers. This technique disrupts a key step in the viral infection process and may potentially be used to engineer virus resistance in a number of economically important plant–viral pathosystems. Furthermore, the general public may be more amenable to the ‘intragenic’ nature of this approach because the transferred coding region is modified from a gene in the target crop rather than from a distant species.</jats:p> Engineering virus resistance using a modified potato gene Plant Biotechnology Journal |
| spellingShingle | Cavatorta, Jason, Perez, Kari W., Gray, Stewart M., Van Eck, Joyce, Yeam, Inhwa, Jahn, Molly, Plant Biotechnology Journal, Engineering virus resistance using a modified potato gene, Plant Science, Agronomy and Crop Science, Biotechnology |
| title | Engineering virus resistance using a modified potato gene |
| title_full | Engineering virus resistance using a modified potato gene |
| title_fullStr | Engineering virus resistance using a modified potato gene |
| title_full_unstemmed | Engineering virus resistance using a modified potato gene |
| title_short | Engineering virus resistance using a modified potato gene |
| title_sort | engineering virus resistance using a modified potato gene |
| title_unstemmed | Engineering virus resistance using a modified potato gene |
| topic | Plant Science, Agronomy and Crop Science, Biotechnology |
| url | http://dx.doi.org/10.1111/j.1467-7652.2011.00622.x |