Eintrag weiter verarbeiten
Soluble fibrin consists of fibrin oligomers of heterogeneous distribution
Gespeichert in:
Zeitschriftentitel: | European Journal of Biochemistry |
---|---|
Personen und Körperschaften: | , , |
In: | European Journal of Biochemistry, 155, 1986, 3, S. 583-588 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Wiley
|
Schlagwörter: |
author_facet |
RÖTKER, Jürgen PREISSNER, Klaus T. MÜLLER‐BERGHAUS, Gert RÖTKER, Jürgen PREISSNER, Klaus T. MÜLLER‐BERGHAUS, Gert |
---|---|
author |
RÖTKER, Jürgen PREISSNER, Klaus T. MÜLLER‐BERGHAUS, Gert |
spellingShingle |
RÖTKER, Jürgen PREISSNER, Klaus T. MÜLLER‐BERGHAUS, Gert European Journal of Biochemistry Soluble fibrin consists of fibrin oligomers of heterogeneous distribution Biochemistry |
author_sort |
rötker, jürgen |
spelling |
RÖTKER, Jürgen PREISSNER, Klaus T. MÜLLER‐BERGHAUS, Gert 0014-2956 1432-1033 Wiley Biochemistry http://dx.doi.org/10.1111/j.1432-1033.1986.tb09528.x <jats:p>Soluble fibrin is observed in patients with intravascular coagulation and represents an intermediary product of conversion of fibrin monomers into a fibrin clot whereby the presence of fibrinogen may suppress fibrin clot formation. The interactions between fibrin and fibrinogen and the occurrence of fibrin oligomers in soluble fibrin were studied by sucrose density ultracentrifugation. Different concentrations of soluble fibrin, prepared by mixing <jats:sup>125</jats:sup>I‐fibrin (24 nM – 1.5 μM) with a constant concentration of <jats:sup>131</jats:sup>I‐fibrinogen (6 μM) were analyzed at 37°C in stable linear sucrose density gradients containing a uniform concentration of unlabelled fibrinogen (6 μM) and calcium ions in order to mimic the physiological situation. At any fibrin concentration, <jats:sup>125</jats:sup>I‐fibrin sedimented faster than <jats:sup>131</jats:sup>I‐fibrinogen through 5 – 30% (w/v) sucrose gradients. Sedimentation rates of fibrin increased from 9 S to 23 S depending on the initial fibrin concentration. The relative amount of residual fibrin monomer not incorporated into oligomers was calculated from the sedimentation profiles. At any fibrin concentration, the portion of free monomer was always more than twofold higher for batroxobin‐generated (desAA‐) fibrin than for thrombin‐generated (desAABB‐) fibrin. Apparent association constants for desAABB‐fibrin were 3–10 times higher than those for desAA‐fibrin indicating a stronger interaction between monomers of the former type of fibrin. In the presence of excess fibrinogen the predominant species in soluble desAA‐fibrin were monomers and dimers, whereas dimers, trimers and higher‐molecular‐mass oligomers were present in soluble desAABB‐fibrin. Strong interactions between both types of fibrin were demonstrated from their cosedimentation, whereby the size of these copolymers was shown to be governed by the oligomer size of the desAABB‐fibrin type. These results provide evidence for the occurrence of differently sized oligomers of fibrin in soluble fibrin and for the concept of a cooperative polymerization process between both types of fibrin devoid of any stable complexes between fibrin and fibrinogen.</jats:p> Soluble fibrin consists of fibrin oligomers of heterogeneous distribution European Journal of Biochemistry |
doi_str_mv |
10.1111/j.1432-1033.1986.tb09528.x |
facet_avail |
Online Free |
finc_class_facet |
Chemie und Pharmazie |
format |
ElectronicArticle |
fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTExMS9qLjE0MzItMTAzMy4xOTg2LnRiMDk1MjgueA |
id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTExMS9qLjE0MzItMTAzMy4xOTg2LnRiMDk1MjgueA |
institution |
DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Zwi2 |
imprint |
Wiley, 1986 |
imprint_str_mv |
Wiley, 1986 |
issn |
0014-2956 1432-1033 |
issn_str_mv |
0014-2956 1432-1033 |
language |
English |
mega_collection |
Wiley (CrossRef) |
match_str |
rotker1986solublefibrinconsistsoffibrinoligomersofheterogeneousdistribution |
publishDateSort |
1986 |
publisher |
Wiley |
recordtype |
ai |
record_format |
ai |
series |
European Journal of Biochemistry |
source_id |
49 |
title |
Soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
title_unstemmed |
Soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
title_full |
Soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
title_fullStr |
Soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
title_full_unstemmed |
Soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
title_short |
Soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
title_sort |
soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
topic |
Biochemistry |
url |
http://dx.doi.org/10.1111/j.1432-1033.1986.tb09528.x |
publishDate |
1986 |
physical |
583-588 |
description |
<jats:p>Soluble fibrin is observed in patients with intravascular coagulation and represents an intermediary product of conversion of fibrin monomers into a fibrin clot whereby the presence of fibrinogen may suppress fibrin clot formation. The interactions between fibrin and fibrinogen and the occurrence of fibrin oligomers in soluble fibrin were studied by sucrose density ultracentrifugation. Different concentrations of soluble fibrin, prepared by mixing <jats:sup>125</jats:sup>I‐fibrin (24 nM – 1.5 μM) with a constant concentration of <jats:sup>131</jats:sup>I‐fibrinogen (6 μM) were analyzed at 37°C in stable linear sucrose density gradients containing a uniform concentration of unlabelled fibrinogen (6 μM) and calcium ions in order to mimic the physiological situation. At any fibrin concentration, <jats:sup>125</jats:sup>I‐fibrin sedimented faster than <jats:sup>131</jats:sup>I‐fibrinogen through 5 – 30% (w/v) sucrose gradients. Sedimentation rates of fibrin increased from 9 S to 23 S depending on the initial fibrin concentration. The relative amount of residual fibrin monomer not incorporated into oligomers was calculated from the sedimentation profiles. At any fibrin concentration, the portion of free monomer was always more than twofold higher for batroxobin‐generated (desAA‐) fibrin than for thrombin‐generated (desAABB‐) fibrin. Apparent association constants for desAABB‐fibrin were 3–10 times higher than those for desAA‐fibrin indicating a stronger interaction between monomers of the former type of fibrin. In the presence of excess fibrinogen the predominant species in soluble desAA‐fibrin were monomers and dimers, whereas dimers, trimers and higher‐molecular‐mass oligomers were present in soluble desAABB‐fibrin. Strong interactions between both types of fibrin were demonstrated from their cosedimentation, whereby the size of these copolymers was shown to be governed by the oligomer size of the desAABB‐fibrin type. These results provide evidence for the occurrence of differently sized oligomers of fibrin in soluble fibrin and for the concept of a cooperative polymerization process between both types of fibrin devoid of any stable complexes between fibrin and fibrinogen.</jats:p> |
container_issue |
3 |
container_start_page |
583 |
container_title |
European Journal of Biochemistry |
container_volume |
155 |
format_de105 |
Article, E-Article |
format_de14 |
Article, E-Article |
format_de15 |
Article, E-Article |
format_de520 |
Article, E-Article |
format_de540 |
Article, E-Article |
format_dech1 |
Article, E-Article |
format_ded117 |
Article, E-Article |
format_degla1 |
E-Article |
format_del152 |
Buch |
format_del189 |
Article, E-Article |
format_dezi4 |
Article |
format_dezwi2 |
Article, E-Article |
format_finc |
Article, E-Article |
format_nrw |
Article, E-Article |
_version_ |
1792339811708698624 |
geogr_code |
not assigned |
last_indexed |
2024-03-01T15:53:35.41Z |
geogr_code_person |
not assigned |
openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Soluble+fibrin+consists+of+fibrin+oligomers+of+heterogeneous+distribution&rft.date=1986-03-01&genre=article&issn=1432-1033&volume=155&issue=3&spage=583&epage=588&pages=583-588&jtitle=European+Journal+of+Biochemistry&atitle=Soluble+fibrin+consists+of+fibrin+oligomers+of+heterogeneous+distribution&aulast=M%C3%9CLLER%E2%80%90BERGHAUS&aufirst=Gert&rft_id=info%3Adoi%2F10.1111%2Fj.1432-1033.1986.tb09528.x&rft.language%5B0%5D=eng |
SOLR | |
_version_ | 1792339811708698624 |
author | RÖTKER, Jürgen, PREISSNER, Klaus T., MÜLLER‐BERGHAUS, Gert |
author_facet | RÖTKER, Jürgen, PREISSNER, Klaus T., MÜLLER‐BERGHAUS, Gert, RÖTKER, Jürgen, PREISSNER, Klaus T., MÜLLER‐BERGHAUS, Gert |
author_sort | rötker, jürgen |
container_issue | 3 |
container_start_page | 583 |
container_title | European Journal of Biochemistry |
container_volume | 155 |
description | <jats:p>Soluble fibrin is observed in patients with intravascular coagulation and represents an intermediary product of conversion of fibrin monomers into a fibrin clot whereby the presence of fibrinogen may suppress fibrin clot formation. The interactions between fibrin and fibrinogen and the occurrence of fibrin oligomers in soluble fibrin were studied by sucrose density ultracentrifugation. Different concentrations of soluble fibrin, prepared by mixing <jats:sup>125</jats:sup>I‐fibrin (24 nM – 1.5 μM) with a constant concentration of <jats:sup>131</jats:sup>I‐fibrinogen (6 μM) were analyzed at 37°C in stable linear sucrose density gradients containing a uniform concentration of unlabelled fibrinogen (6 μM) and calcium ions in order to mimic the physiological situation. At any fibrin concentration, <jats:sup>125</jats:sup>I‐fibrin sedimented faster than <jats:sup>131</jats:sup>I‐fibrinogen through 5 – 30% (w/v) sucrose gradients. Sedimentation rates of fibrin increased from 9 S to 23 S depending on the initial fibrin concentration. The relative amount of residual fibrin monomer not incorporated into oligomers was calculated from the sedimentation profiles. At any fibrin concentration, the portion of free monomer was always more than twofold higher for batroxobin‐generated (desAA‐) fibrin than for thrombin‐generated (desAABB‐) fibrin. Apparent association constants for desAABB‐fibrin were 3–10 times higher than those for desAA‐fibrin indicating a stronger interaction between monomers of the former type of fibrin. In the presence of excess fibrinogen the predominant species in soluble desAA‐fibrin were monomers and dimers, whereas dimers, trimers and higher‐molecular‐mass oligomers were present in soluble desAABB‐fibrin. Strong interactions between both types of fibrin were demonstrated from their cosedimentation, whereby the size of these copolymers was shown to be governed by the oligomer size of the desAABB‐fibrin type. These results provide evidence for the occurrence of differently sized oligomers of fibrin in soluble fibrin and for the concept of a cooperative polymerization process between both types of fibrin devoid of any stable complexes between fibrin and fibrinogen.</jats:p> |
doi_str_mv | 10.1111/j.1432-1033.1986.tb09528.x |
facet_avail | Online, Free |
finc_class_facet | Chemie und Pharmazie |
format | ElectronicArticle |
format_de105 | Article, E-Article |
format_de14 | Article, E-Article |
format_de15 | Article, E-Article |
format_de520 | Article, E-Article |
format_de540 | Article, E-Article |
format_dech1 | Article, E-Article |
format_ded117 | Article, E-Article |
format_degla1 | E-Article |
format_del152 | Buch |
format_del189 | Article, E-Article |
format_dezi4 | Article |
format_dezwi2 | Article, E-Article |
format_finc | Article, E-Article |
format_nrw | Article, E-Article |
geogr_code | not assigned |
geogr_code_person | not assigned |
id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTExMS9qLjE0MzItMTAzMy4xOTg2LnRiMDk1MjgueA |
imprint | Wiley, 1986 |
imprint_str_mv | Wiley, 1986 |
institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2 |
issn | 0014-2956, 1432-1033 |
issn_str_mv | 0014-2956, 1432-1033 |
language | English |
last_indexed | 2024-03-01T15:53:35.41Z |
match_str | rotker1986solublefibrinconsistsoffibrinoligomersofheterogeneousdistribution |
mega_collection | Wiley (CrossRef) |
physical | 583-588 |
publishDate | 1986 |
publishDateSort | 1986 |
publisher | Wiley |
record_format | ai |
recordtype | ai |
series | European Journal of Biochemistry |
source_id | 49 |
spelling | RÖTKER, Jürgen PREISSNER, Klaus T. MÜLLER‐BERGHAUS, Gert 0014-2956 1432-1033 Wiley Biochemistry http://dx.doi.org/10.1111/j.1432-1033.1986.tb09528.x <jats:p>Soluble fibrin is observed in patients with intravascular coagulation and represents an intermediary product of conversion of fibrin monomers into a fibrin clot whereby the presence of fibrinogen may suppress fibrin clot formation. The interactions between fibrin and fibrinogen and the occurrence of fibrin oligomers in soluble fibrin were studied by sucrose density ultracentrifugation. Different concentrations of soluble fibrin, prepared by mixing <jats:sup>125</jats:sup>I‐fibrin (24 nM – 1.5 μM) with a constant concentration of <jats:sup>131</jats:sup>I‐fibrinogen (6 μM) were analyzed at 37°C in stable linear sucrose density gradients containing a uniform concentration of unlabelled fibrinogen (6 μM) and calcium ions in order to mimic the physiological situation. At any fibrin concentration, <jats:sup>125</jats:sup>I‐fibrin sedimented faster than <jats:sup>131</jats:sup>I‐fibrinogen through 5 – 30% (w/v) sucrose gradients. Sedimentation rates of fibrin increased from 9 S to 23 S depending on the initial fibrin concentration. The relative amount of residual fibrin monomer not incorporated into oligomers was calculated from the sedimentation profiles. At any fibrin concentration, the portion of free monomer was always more than twofold higher for batroxobin‐generated (desAA‐) fibrin than for thrombin‐generated (desAABB‐) fibrin. Apparent association constants for desAABB‐fibrin were 3–10 times higher than those for desAA‐fibrin indicating a stronger interaction between monomers of the former type of fibrin. In the presence of excess fibrinogen the predominant species in soluble desAA‐fibrin were monomers and dimers, whereas dimers, trimers and higher‐molecular‐mass oligomers were present in soluble desAABB‐fibrin. Strong interactions between both types of fibrin were demonstrated from their cosedimentation, whereby the size of these copolymers was shown to be governed by the oligomer size of the desAABB‐fibrin type. These results provide evidence for the occurrence of differently sized oligomers of fibrin in soluble fibrin and for the concept of a cooperative polymerization process between both types of fibrin devoid of any stable complexes between fibrin and fibrinogen.</jats:p> Soluble fibrin consists of fibrin oligomers of heterogeneous distribution European Journal of Biochemistry |
spellingShingle | RÖTKER, Jürgen, PREISSNER, Klaus T., MÜLLER‐BERGHAUS, Gert, European Journal of Biochemistry, Soluble fibrin consists of fibrin oligomers of heterogeneous distribution, Biochemistry |
title | Soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
title_full | Soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
title_fullStr | Soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
title_full_unstemmed | Soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
title_short | Soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
title_sort | soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
title_unstemmed | Soluble fibrin consists of fibrin oligomers of heterogeneous distribution |
topic | Biochemistry |
url | http://dx.doi.org/10.1111/j.1432-1033.1986.tb09528.x |