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Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone
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| Zeitschriftentitel: | European Journal of Biochemistry |
|---|---|
| Personen und Körperschaften: | , |
| In: | European Journal of Biochemistry, 3, 1968, 4, S. 488-491 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Moron, J. Polonsky, J. Moron, J. Polonsky, J. |
|---|---|
| author |
Moron, J. Polonsky, J. |
| spellingShingle |
Moron, J. Polonsky, J. European Journal of Biochemistry Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone Biochemistry |
| author_sort |
moron, j. |
| spelling |
Moron, J. Polonsky, J. 0014-2956 1432-1033 Wiley Biochemistry http://dx.doi.org/10.1111/j.1432-1033.1967.tb19557.x <jats:p>2‐Hydroxy‐2‐methylbutyric acid (III) occurs esterified to glaucarubolone (II) in glaucarubinone (I), the bitter principle of <jats:italic>Simaruba glauca</jats:italic> (Simarubaceae).</jats:p><jats:p>No experimental results concerning the biosynthesis of this acid have yet been reported.</jats:p><jats:p>The biogenetic precursor of 2‐hydroxy‐2‐methylbutyric acid might be either mevalonic acid, acetoacetate, or isoleucine.</jats:p><jats:p>We have shown previously that mevalonic acid is not incorporated into the 2‐hydroxy‐2‐methylbutyrate component of glaucarubinone (I) although it is into glaucarubolone (II).</jats:p><jats:p>We now report our feeding experiments with two batches of young shoots of <jats:italic>Simaruba glauca</jats:italic> using <jats:sc>l</jats:sc>‐[U‐<jats:sup>14</jats:sup>C]isoleucine and sodium [1‐<jats:sup>14</jats:sup>C]acetate.</jats:p><jats:p>After 8 days the bitter principles were isolated and the total incorporation of radioactivity determined. The radioactive glaucarubinone (I) was hydrolysed to glaucarubolone (II) and 2‐hydroxy 2‐methyl butyric acid (III). This acid, when obtained from glaucarubolone after incorporation of acetate, has been found to be weakly labelled. In contrast, it carries essentially all the activity of glaucarubinone, when isolated after incorporation of isoleucine.</jats:p><jats:p>These results establish the specific incorporation of isoleucine into the 2‐hydroxy 2‐methyl butyroyl part of glaucarubinone.</jats:p> Incorporation spécifique de la L‐[U‐<sup>14</sup>C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone European Journal of Biochemistry |
| doi_str_mv |
10.1111/j.1432-1033.1967.tb19557.x |
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Online Free |
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Chemie und Pharmazie |
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Wiley, 1968 |
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Wiley, 1968 |
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0014-2956 1432-1033 |
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0014-2956 1432-1033 |
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moron1968incorporationspecifiquedelalu14cisoleucinedanslegroupementhydroxy2methyl2butyroyledelaglaucarubinone |
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1968 |
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Wiley |
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ai |
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ai |
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European Journal of Biochemistry |
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49 |
| title |
Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| title_unstemmed |
Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| title_full |
Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| title_fullStr |
Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| title_full_unstemmed |
Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| title_short |
Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| title_sort |
incorporation spécifique de la l‐[u‐<sup>14</sup>c] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| topic |
Biochemistry |
| url |
http://dx.doi.org/10.1111/j.1432-1033.1967.tb19557.x |
| publishDate |
1968 |
| physical |
488-491 |
| description |
<jats:p>2‐Hydroxy‐2‐methylbutyric acid (III) occurs esterified to glaucarubolone (II) in glaucarubinone (I), the bitter principle of <jats:italic>Simaruba glauca</jats:italic> (Simarubaceae).</jats:p><jats:p>No experimental results concerning the biosynthesis of this acid have yet been reported.</jats:p><jats:p>The biogenetic precursor of 2‐hydroxy‐2‐methylbutyric acid might be either mevalonic acid, acetoacetate, or isoleucine.</jats:p><jats:p>We have shown previously that mevalonic acid is not incorporated into the 2‐hydroxy‐2‐methylbutyrate component of glaucarubinone (I) although it is into glaucarubolone (II).</jats:p><jats:p>We now report our feeding experiments with two batches of young shoots of <jats:italic>Simaruba glauca</jats:italic> using <jats:sc>l</jats:sc>‐[U‐<jats:sup>14</jats:sup>C]isoleucine and sodium [1‐<jats:sup>14</jats:sup>C]acetate.</jats:p><jats:p>After 8 days the bitter principles were isolated and the total incorporation of radioactivity determined. The radioactive glaucarubinone (I) was hydrolysed to glaucarubolone (II) and 2‐hydroxy 2‐methyl butyric acid (III). This acid, when obtained from glaucarubolone after incorporation of acetate, has been found to be weakly labelled. In contrast, it carries essentially all the activity of glaucarubinone, when isolated after incorporation of isoleucine.</jats:p><jats:p>These results establish the specific incorporation of isoleucine into the 2‐hydroxy 2‐methyl butyroyl part of glaucarubinone.</jats:p> |
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| author | Moron, J., Polonsky, J. |
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| author_sort | moron, j. |
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| description | <jats:p>2‐Hydroxy‐2‐methylbutyric acid (III) occurs esterified to glaucarubolone (II) in glaucarubinone (I), the bitter principle of <jats:italic>Simaruba glauca</jats:italic> (Simarubaceae).</jats:p><jats:p>No experimental results concerning the biosynthesis of this acid have yet been reported.</jats:p><jats:p>The biogenetic precursor of 2‐hydroxy‐2‐methylbutyric acid might be either mevalonic acid, acetoacetate, or isoleucine.</jats:p><jats:p>We have shown previously that mevalonic acid is not incorporated into the 2‐hydroxy‐2‐methylbutyrate component of glaucarubinone (I) although it is into glaucarubolone (II).</jats:p><jats:p>We now report our feeding experiments with two batches of young shoots of <jats:italic>Simaruba glauca</jats:italic> using <jats:sc>l</jats:sc>‐[U‐<jats:sup>14</jats:sup>C]isoleucine and sodium [1‐<jats:sup>14</jats:sup>C]acetate.</jats:p><jats:p>After 8 days the bitter principles were isolated and the total incorporation of radioactivity determined. The radioactive glaucarubinone (I) was hydrolysed to glaucarubolone (II) and 2‐hydroxy 2‐methyl butyric acid (III). This acid, when obtained from glaucarubolone after incorporation of acetate, has been found to be weakly labelled. In contrast, it carries essentially all the activity of glaucarubinone, when isolated after incorporation of isoleucine.</jats:p><jats:p>These results establish the specific incorporation of isoleucine into the 2‐hydroxy 2‐methyl butyroyl part of glaucarubinone.</jats:p> |
| doi_str_mv | 10.1111/j.1432-1033.1967.tb19557.x |
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| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTExMS9qLjE0MzItMTAzMy4xOTY3LnRiMTk1NTcueA |
| imprint | Wiley, 1968 |
| imprint_str_mv | Wiley, 1968 |
| institution | DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
| issn | 0014-2956, 1432-1033 |
| issn_str_mv | 0014-2956, 1432-1033 |
| language | English |
| last_indexed | 2024-03-01T14:43:17.936Z |
| match_str | moron1968incorporationspecifiquedelalu14cisoleucinedanslegroupementhydroxy2methyl2butyroyledelaglaucarubinone |
| mega_collection | Wiley (CrossRef) |
| physical | 488-491 |
| publishDate | 1968 |
| publishDateSort | 1968 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | European Journal of Biochemistry |
| source_id | 49 |
| spelling | Moron, J. Polonsky, J. 0014-2956 1432-1033 Wiley Biochemistry http://dx.doi.org/10.1111/j.1432-1033.1967.tb19557.x <jats:p>2‐Hydroxy‐2‐methylbutyric acid (III) occurs esterified to glaucarubolone (II) in glaucarubinone (I), the bitter principle of <jats:italic>Simaruba glauca</jats:italic> (Simarubaceae).</jats:p><jats:p>No experimental results concerning the biosynthesis of this acid have yet been reported.</jats:p><jats:p>The biogenetic precursor of 2‐hydroxy‐2‐methylbutyric acid might be either mevalonic acid, acetoacetate, or isoleucine.</jats:p><jats:p>We have shown previously that mevalonic acid is not incorporated into the 2‐hydroxy‐2‐methylbutyrate component of glaucarubinone (I) although it is into glaucarubolone (II).</jats:p><jats:p>We now report our feeding experiments with two batches of young shoots of <jats:italic>Simaruba glauca</jats:italic> using <jats:sc>l</jats:sc>‐[U‐<jats:sup>14</jats:sup>C]isoleucine and sodium [1‐<jats:sup>14</jats:sup>C]acetate.</jats:p><jats:p>After 8 days the bitter principles were isolated and the total incorporation of radioactivity determined. The radioactive glaucarubinone (I) was hydrolysed to glaucarubolone (II) and 2‐hydroxy 2‐methyl butyric acid (III). This acid, when obtained from glaucarubolone after incorporation of acetate, has been found to be weakly labelled. In contrast, it carries essentially all the activity of glaucarubinone, when isolated after incorporation of isoleucine.</jats:p><jats:p>These results establish the specific incorporation of isoleucine into the 2‐hydroxy 2‐methyl butyroyl part of glaucarubinone.</jats:p> Incorporation spécifique de la L‐[U‐<sup>14</sup>C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone European Journal of Biochemistry |
| spellingShingle | Moron, J., Polonsky, J., European Journal of Biochemistry, Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone, Biochemistry |
| title | Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| title_full | Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| title_fullStr | Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| title_full_unstemmed | Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| title_short | Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| title_sort | incorporation spécifique de la l‐[u‐<sup>14</sup>c] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| title_unstemmed | Incorporation spécifique de la L‐[U‐14C] isoleucine dans le groupement hydroxy‐2 méthyl‐2 butyroyle de la glaucarubinone |
| topic | Biochemistry |
| url | http://dx.doi.org/10.1111/j.1432-1033.1967.tb19557.x |