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microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner)
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| Zeitschriftentitel: | Insect Molecular Biology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Insect Molecular Biology, 27, 2018, 3, S. 279-294 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Yu, T. Li, X. Coates, B.‐S. Zhang, Q. Siegfried, B.‐D. Zhou, X. Yu, T. Li, X. Coates, B.‐S. Zhang, Q. Siegfried, B.‐D. Zhou, X. |
|---|---|
| author |
Yu, T. Li, X. Coates, B.‐S. Zhang, Q. Siegfried, B.‐D. Zhou, X. |
| spellingShingle |
Yu, T. Li, X. Coates, B.‐S. Zhang, Q. Siegfried, B.‐D. Zhou, X. Insect Molecular Biology microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner) Insect Science Genetics Molecular Biology |
| author_sort |
yu, t. |
| spelling |
Yu, T. Li, X. Coates, B.‐S. Zhang, Q. Siegfried, B.‐D. Zhou, X. 0962-1075 1365-2583 Wiley Insect Science Genetics Molecular Biology http://dx.doi.org/10.1111/imb.12376 <jats:title>Abstract</jats:title><jats:p>Transgenic maize hybrids that express insecticidal <jats:italic>Bacillus thuringiensis</jats:italic> (<jats:italic>Bt</jats:italic>) crystalline (Cry) protein toxins effectively protect against the European corn borer, <jats:italic>Ostrinia nubilalis</jats:italic>, a devastating maize pest. Field monitoring and laboratory selections have detected varying levels of <jats:italic>O. nubilalis</jats:italic> resistance to Cry1Ab toxin. MicroRNAs (miRNAs) are short noncoding RNAs that are involved in post‐transcriptional gene regulation. Their potential roles in the evolution of <jats:italic>Bt</jats:italic> resistance, however, remain largely unknown. Sequencing of small RNA libraries from the midgut of Cry1Ab‐susceptible and resistant <jats:italic>O. nubilalis</jats:italic> larvae resulted in the discovery of 277 miRNAs, including 248 conserved and 29 novel. Comparative analyses of miRNA expression profiles between the laboratory strains predicted 26 and nine significantly up‐ and down‐regulated transcripts, respectively, in the midgut of Cry1Ab resistant larvae. Amongst 15 differentially regulated miRNAs examined by quantitative real‐time PCR, nine (60%) were validated as cosegregating with Cry1Ab resistance in a backcross progeny. Differentially expressed miRNAs were predicted to affect transcripts involved in cell membrane components with functions in metabolism and binding, and the putative <jats:italic>Bt</jats:italic>‐resistance genes <jats:italic>aminopeptidase N</jats:italic> and <jats:italic>cadherin</jats:italic>. These results lay the foundation for future investigation of the potential role of miRNAs in the evolution of <jats:italic>Bt</jats:italic> resistance.</jats:p> microRNA profiling between <i>Bacillus thuringiensis</i> Cry1Ab‐susceptible and ‐resistant European corn borer, <i>Ostrinia nubilalis</i> (Hübner) Insect Molecular Biology |
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| title |
microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner) |
| title_unstemmed |
microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner) |
| title_full |
microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner) |
| title_fullStr |
microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner) |
| title_full_unstemmed |
microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner) |
| title_short |
microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner) |
| title_sort |
microrna profiling between <i>bacillus thuringiensis</i> cry1ab‐susceptible and ‐resistant european corn borer, <i>ostrinia nubilalis</i> (hübner) |
| topic |
Insect Science Genetics Molecular Biology |
| url |
http://dx.doi.org/10.1111/imb.12376 |
| publishDate |
2018 |
| physical |
279-294 |
| description |
<jats:title>Abstract</jats:title><jats:p>Transgenic maize hybrids that express insecticidal <jats:italic>Bacillus thuringiensis</jats:italic> (<jats:italic>Bt</jats:italic>) crystalline (Cry) protein toxins effectively protect against the European corn borer, <jats:italic>Ostrinia nubilalis</jats:italic>, a devastating maize pest. Field monitoring and laboratory selections have detected varying levels of <jats:italic>O. nubilalis</jats:italic> resistance to Cry1Ab toxin. MicroRNAs (miRNAs) are short noncoding RNAs that are involved in post‐transcriptional gene regulation. Their potential roles in the evolution of <jats:italic>Bt</jats:italic> resistance, however, remain largely unknown. Sequencing of small RNA libraries from the midgut of Cry1Ab‐susceptible and resistant <jats:italic>O. nubilalis</jats:italic> larvae resulted in the discovery of 277 miRNAs, including 248 conserved and 29 novel. Comparative analyses of miRNA expression profiles between the laboratory strains predicted 26 and nine significantly up‐ and down‐regulated transcripts, respectively, in the midgut of Cry1Ab resistant larvae. Amongst 15 differentially regulated miRNAs examined by quantitative real‐time PCR, nine (60%) were validated as cosegregating with Cry1Ab resistance in a backcross progeny. Differentially expressed miRNAs were predicted to affect transcripts involved in cell membrane components with functions in metabolism and binding, and the putative <jats:italic>Bt</jats:italic>‐resistance genes <jats:italic>aminopeptidase N</jats:italic> and <jats:italic>cadherin</jats:italic>. These results lay the foundation for future investigation of the potential role of miRNAs in the evolution of <jats:italic>Bt</jats:italic> resistance.</jats:p> |
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| author | Yu, T., Li, X., Coates, B.‐S., Zhang, Q., Siegfried, B.‐D., Zhou, X. |
| author_facet | Yu, T., Li, X., Coates, B.‐S., Zhang, Q., Siegfried, B.‐D., Zhou, X., Yu, T., Li, X., Coates, B.‐S., Zhang, Q., Siegfried, B.‐D., Zhou, X. |
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| description | <jats:title>Abstract</jats:title><jats:p>Transgenic maize hybrids that express insecticidal <jats:italic>Bacillus thuringiensis</jats:italic> (<jats:italic>Bt</jats:italic>) crystalline (Cry) protein toxins effectively protect against the European corn borer, <jats:italic>Ostrinia nubilalis</jats:italic>, a devastating maize pest. Field monitoring and laboratory selections have detected varying levels of <jats:italic>O. nubilalis</jats:italic> resistance to Cry1Ab toxin. MicroRNAs (miRNAs) are short noncoding RNAs that are involved in post‐transcriptional gene regulation. Their potential roles in the evolution of <jats:italic>Bt</jats:italic> resistance, however, remain largely unknown. Sequencing of small RNA libraries from the midgut of Cry1Ab‐susceptible and resistant <jats:italic>O. nubilalis</jats:italic> larvae resulted in the discovery of 277 miRNAs, including 248 conserved and 29 novel. Comparative analyses of miRNA expression profiles between the laboratory strains predicted 26 and nine significantly up‐ and down‐regulated transcripts, respectively, in the midgut of Cry1Ab resistant larvae. Amongst 15 differentially regulated miRNAs examined by quantitative real‐time PCR, nine (60%) were validated as cosegregating with Cry1Ab resistance in a backcross progeny. Differentially expressed miRNAs were predicted to affect transcripts involved in cell membrane components with functions in metabolism and binding, and the putative <jats:italic>Bt</jats:italic>‐resistance genes <jats:italic>aminopeptidase N</jats:italic> and <jats:italic>cadherin</jats:italic>. These results lay the foundation for future investigation of the potential role of miRNAs in the evolution of <jats:italic>Bt</jats:italic> resistance.</jats:p> |
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| spelling | Yu, T. Li, X. Coates, B.‐S. Zhang, Q. Siegfried, B.‐D. Zhou, X. 0962-1075 1365-2583 Wiley Insect Science Genetics Molecular Biology http://dx.doi.org/10.1111/imb.12376 <jats:title>Abstract</jats:title><jats:p>Transgenic maize hybrids that express insecticidal <jats:italic>Bacillus thuringiensis</jats:italic> (<jats:italic>Bt</jats:italic>) crystalline (Cry) protein toxins effectively protect against the European corn borer, <jats:italic>Ostrinia nubilalis</jats:italic>, a devastating maize pest. Field monitoring and laboratory selections have detected varying levels of <jats:italic>O. nubilalis</jats:italic> resistance to Cry1Ab toxin. MicroRNAs (miRNAs) are short noncoding RNAs that are involved in post‐transcriptional gene regulation. Their potential roles in the evolution of <jats:italic>Bt</jats:italic> resistance, however, remain largely unknown. Sequencing of small RNA libraries from the midgut of Cry1Ab‐susceptible and resistant <jats:italic>O. nubilalis</jats:italic> larvae resulted in the discovery of 277 miRNAs, including 248 conserved and 29 novel. Comparative analyses of miRNA expression profiles between the laboratory strains predicted 26 and nine significantly up‐ and down‐regulated transcripts, respectively, in the midgut of Cry1Ab resistant larvae. Amongst 15 differentially regulated miRNAs examined by quantitative real‐time PCR, nine (60%) were validated as cosegregating with Cry1Ab resistance in a backcross progeny. Differentially expressed miRNAs were predicted to affect transcripts involved in cell membrane components with functions in metabolism and binding, and the putative <jats:italic>Bt</jats:italic>‐resistance genes <jats:italic>aminopeptidase N</jats:italic> and <jats:italic>cadherin</jats:italic>. These results lay the foundation for future investigation of the potential role of miRNAs in the evolution of <jats:italic>Bt</jats:italic> resistance.</jats:p> microRNA profiling between <i>Bacillus thuringiensis</i> Cry1Ab‐susceptible and ‐resistant European corn borer, <i>Ostrinia nubilalis</i> (Hübner) Insect Molecular Biology |
| spellingShingle | Yu, T., Li, X., Coates, B.‐S., Zhang, Q., Siegfried, B.‐D., Zhou, X., Insect Molecular Biology, microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner), Insect Science, Genetics, Molecular Biology |
| title | microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner) |
| title_full | microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner) |
| title_fullStr | microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner) |
| title_full_unstemmed | microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner) |
| title_short | microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner) |
| title_sort | microrna profiling between <i>bacillus thuringiensis</i> cry1ab‐susceptible and ‐resistant european corn borer, <i>ostrinia nubilalis</i> (hübner) |
| title_unstemmed | microRNA profiling between Bacillus thuringiensis Cry1Ab‐susceptible and ‐resistant European corn borer, Ostrinia nubilalis (Hübner) |
| topic | Insect Science, Genetics, Molecular Biology |
| url | http://dx.doi.org/10.1111/imb.12376 |