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Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover
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| Zeitschriftentitel: | Insect Molecular Biology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , |
| In: | Insect Molecular Biology, 25, 2016, 4, S. 450-459 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Peng, T. Pan, Y. Gao, X. Xi, J. Zhang, L. Yang, C. Bi, R. Yang, S. Xin, X. Shang, Q. Peng, T. Pan, Y. Gao, X. Xi, J. Zhang, L. Yang, C. Bi, R. Yang, S. Xin, X. Shang, Q. |
|---|---|
| author |
Peng, T. Pan, Y. Gao, X. Xi, J. Zhang, L. Yang, C. Bi, R. Yang, S. Xin, X. Shang, Q. |
| spellingShingle |
Peng, T. Pan, Y. Gao, X. Xi, J. Zhang, L. Yang, C. Bi, R. Yang, S. Xin, X. Shang, Q. Insect Molecular Biology Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover Insect Science Genetics Molecular Biology |
| author_sort |
peng, t. |
| spelling |
Peng, T. Pan, Y. Gao, X. Xi, J. Zhang, L. Yang, C. Bi, R. Yang, S. Xin, X. Shang, Q. 0962-1075 1365-2583 Wiley Insect Science Genetics Molecular Biology http://dx.doi.org/10.1111/imb.12230 <jats:title>Abstract</jats:title><jats:p>Cotton plants accumulate phytotoxins, such as gossypol and related sesquiterpene aldehydes, to resist insect herbivores. The survival of insects exposed to toxic secondary metabolites depends on the detoxification metabolism mediated by limited groups of cytochrome P450. Gossypol has an antibiotic effect on <jats:italic>Aphis gossypii</jats:italic>, and as the concentrations of gossypol were increased in the present study, the mortality of cotton aphids increased from 4 to 28%. The fecundity of the cotton aphids exposed to gossypol was also significantly reduced compared with the control. The transcriptional levels of <jats:italic>CYP6DA2</jats:italic> in cotton aphids were significantly induced when exposed to gossypol, and knockdown of the <jats:italic>CYP6DA2</jats:italic> transcripts by RNA interference (RNAi) significantly increased the toxicity of gossypol to cotton aphids. To further understand the gossypol regulatory cascade, the 5'‐flanking promoter sequences of <jats:italic>CYP6DA2</jats:italic> were isolated with a genome walker, and the promoter was very active and was inducible by gossypol. Co‐transfection of the <jats:italic>cap ‘n’ collar isoform C</jats:italic> (<jats:italic>CncC</jats:italic>) and <jats:italic>CYP6DA2</jats:italic> promoters dramatically increased the expression of <jats:italic>CYP6DA2</jats:italic>, and suppression of the <jats:italic>CncC</jats:italic> transcripts by RNAi significantly decreased the expression levels of <jats:italic>CYP6DA2</jats:italic>, and significantly increased the toxicity of gossypol to cotton aphids. Thus, the transcriptional regulation of <jats:italic>CYP6DA2</jats:italic> involved the transcriptional factor <jats:italic>CncC</jats:italic>.</jats:p> Cytochrome P450 <i>CYP6DA2</i> regulated by <i>cap ‘n’collar isoform C (CncC)</i> is associated with gossypol tolerance in <i>Aphis gossypii</i> Glover Insect Molecular Biology |
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Wiley, 2016 |
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| title |
Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover |
| title_unstemmed |
Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover |
| title_full |
Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover |
| title_fullStr |
Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover |
| title_full_unstemmed |
Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover |
| title_short |
Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover |
| title_sort |
cytochrome p450 <i>cyp6da2</i> regulated by <i>cap ‘n’collar isoform c (cncc)</i> is associated with gossypol tolerance in <i>aphis gossypii</i> glover |
| topic |
Insect Science Genetics Molecular Biology |
| url |
http://dx.doi.org/10.1111/imb.12230 |
| publishDate |
2016 |
| physical |
450-459 |
| description |
<jats:title>Abstract</jats:title><jats:p>Cotton plants accumulate phytotoxins, such as gossypol and related sesquiterpene aldehydes, to resist insect herbivores. The survival of insects exposed to toxic secondary metabolites depends on the detoxification metabolism mediated by limited groups of cytochrome P450. Gossypol has an antibiotic effect on <jats:italic>Aphis gossypii</jats:italic>, and as the concentrations of gossypol were increased in the present study, the mortality of cotton aphids increased from 4 to 28%. The fecundity of the cotton aphids exposed to gossypol was also significantly reduced compared with the control. The transcriptional levels of <jats:italic>CYP6DA2</jats:italic> in cotton aphids were significantly induced when exposed to gossypol, and knockdown of the <jats:italic>CYP6DA2</jats:italic> transcripts by RNA interference (RNAi) significantly increased the toxicity of gossypol to cotton aphids. To further understand the gossypol regulatory cascade, the 5'‐flanking promoter sequences of <jats:italic>CYP6DA2</jats:italic> were isolated with a genome walker, and the promoter was very active and was inducible by gossypol. Co‐transfection of the <jats:italic>cap ‘n’ collar isoform C</jats:italic> (<jats:italic>CncC</jats:italic>) and <jats:italic>CYP6DA2</jats:italic> promoters dramatically increased the expression of <jats:italic>CYP6DA2</jats:italic>, and suppression of the <jats:italic>CncC</jats:italic> transcripts by RNAi significantly decreased the expression levels of <jats:italic>CYP6DA2</jats:italic>, and significantly increased the toxicity of gossypol to cotton aphids. Thus, the transcriptional regulation of <jats:italic>CYP6DA2</jats:italic> involved the transcriptional factor <jats:italic>CncC</jats:italic>.</jats:p> |
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| author | Peng, T., Pan, Y., Gao, X., Xi, J., Zhang, L., Yang, C., Bi, R., Yang, S., Xin, X., Shang, Q. |
| author_facet | Peng, T., Pan, Y., Gao, X., Xi, J., Zhang, L., Yang, C., Bi, R., Yang, S., Xin, X., Shang, Q., Peng, T., Pan, Y., Gao, X., Xi, J., Zhang, L., Yang, C., Bi, R., Yang, S., Xin, X., Shang, Q. |
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| description | <jats:title>Abstract</jats:title><jats:p>Cotton plants accumulate phytotoxins, such as gossypol and related sesquiterpene aldehydes, to resist insect herbivores. The survival of insects exposed to toxic secondary metabolites depends on the detoxification metabolism mediated by limited groups of cytochrome P450. Gossypol has an antibiotic effect on <jats:italic>Aphis gossypii</jats:italic>, and as the concentrations of gossypol were increased in the present study, the mortality of cotton aphids increased from 4 to 28%. The fecundity of the cotton aphids exposed to gossypol was also significantly reduced compared with the control. The transcriptional levels of <jats:italic>CYP6DA2</jats:italic> in cotton aphids were significantly induced when exposed to gossypol, and knockdown of the <jats:italic>CYP6DA2</jats:italic> transcripts by RNA interference (RNAi) significantly increased the toxicity of gossypol to cotton aphids. To further understand the gossypol regulatory cascade, the 5'‐flanking promoter sequences of <jats:italic>CYP6DA2</jats:italic> were isolated with a genome walker, and the promoter was very active and was inducible by gossypol. Co‐transfection of the <jats:italic>cap ‘n’ collar isoform C</jats:italic> (<jats:italic>CncC</jats:italic>) and <jats:italic>CYP6DA2</jats:italic> promoters dramatically increased the expression of <jats:italic>CYP6DA2</jats:italic>, and suppression of the <jats:italic>CncC</jats:italic> transcripts by RNAi significantly decreased the expression levels of <jats:italic>CYP6DA2</jats:italic>, and significantly increased the toxicity of gossypol to cotton aphids. Thus, the transcriptional regulation of <jats:italic>CYP6DA2</jats:italic> involved the transcriptional factor <jats:italic>CncC</jats:italic>.</jats:p> |
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| imprint | Wiley, 2016 |
| imprint_str_mv | Wiley, 2016 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161 |
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| mega_collection | Wiley (CrossRef) |
| physical | 450-459 |
| publishDate | 2016 |
| publishDateSort | 2016 |
| publisher | Wiley |
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| series | Insect Molecular Biology |
| source_id | 49 |
| spelling | Peng, T. Pan, Y. Gao, X. Xi, J. Zhang, L. Yang, C. Bi, R. Yang, S. Xin, X. Shang, Q. 0962-1075 1365-2583 Wiley Insect Science Genetics Molecular Biology http://dx.doi.org/10.1111/imb.12230 <jats:title>Abstract</jats:title><jats:p>Cotton plants accumulate phytotoxins, such as gossypol and related sesquiterpene aldehydes, to resist insect herbivores. The survival of insects exposed to toxic secondary metabolites depends on the detoxification metabolism mediated by limited groups of cytochrome P450. Gossypol has an antibiotic effect on <jats:italic>Aphis gossypii</jats:italic>, and as the concentrations of gossypol were increased in the present study, the mortality of cotton aphids increased from 4 to 28%. The fecundity of the cotton aphids exposed to gossypol was also significantly reduced compared with the control. The transcriptional levels of <jats:italic>CYP6DA2</jats:italic> in cotton aphids were significantly induced when exposed to gossypol, and knockdown of the <jats:italic>CYP6DA2</jats:italic> transcripts by RNA interference (RNAi) significantly increased the toxicity of gossypol to cotton aphids. To further understand the gossypol regulatory cascade, the 5'‐flanking promoter sequences of <jats:italic>CYP6DA2</jats:italic> were isolated with a genome walker, and the promoter was very active and was inducible by gossypol. Co‐transfection of the <jats:italic>cap ‘n’ collar isoform C</jats:italic> (<jats:italic>CncC</jats:italic>) and <jats:italic>CYP6DA2</jats:italic> promoters dramatically increased the expression of <jats:italic>CYP6DA2</jats:italic>, and suppression of the <jats:italic>CncC</jats:italic> transcripts by RNAi significantly decreased the expression levels of <jats:italic>CYP6DA2</jats:italic>, and significantly increased the toxicity of gossypol to cotton aphids. Thus, the transcriptional regulation of <jats:italic>CYP6DA2</jats:italic> involved the transcriptional factor <jats:italic>CncC</jats:italic>.</jats:p> Cytochrome P450 <i>CYP6DA2</i> regulated by <i>cap ‘n’collar isoform C (CncC)</i> is associated with gossypol tolerance in <i>Aphis gossypii</i> Glover Insect Molecular Biology |
| spellingShingle | Peng, T., Pan, Y., Gao, X., Xi, J., Zhang, L., Yang, C., Bi, R., Yang, S., Xin, X., Shang, Q., Insect Molecular Biology, Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover, Insect Science, Genetics, Molecular Biology |
| title | Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover |
| title_full | Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover |
| title_fullStr | Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover |
| title_full_unstemmed | Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover |
| title_short | Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover |
| title_sort | cytochrome p450 <i>cyp6da2</i> regulated by <i>cap ‘n’collar isoform c (cncc)</i> is associated with gossypol tolerance in <i>aphis gossypii</i> glover |
| title_unstemmed | Cytochrome P450 CYP6DA2 regulated by cap ‘n’collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover |
| topic | Insect Science, Genetics, Molecular Biology |
| url | http://dx.doi.org/10.1111/imb.12230 |