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The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid
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| Zeitschriftentitel: | Insect Molecular Biology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Insect Molecular Biology, 29, 2020, 1, S. 92-103 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Ma, Z. Zhang, Y. You, C. Zeng, X. Gao, X. Ma, Z. Zhang, Y. You, C. Zeng, X. Gao, X. |
|---|---|
| author |
Ma, Z. Zhang, Y. You, C. Zeng, X. Gao, X. |
| spellingShingle |
Ma, Z. Zhang, Y. You, C. Zeng, X. Gao, X. Insect Molecular Biology The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid Insect Science Genetics Molecular Biology |
| author_sort |
ma, z. |
| spelling |
Ma, Z. Zhang, Y. You, C. Zeng, X. Gao, X. 0962-1075 1365-2583 Wiley Insect Science Genetics Molecular Biology http://dx.doi.org/10.1111/imb.12615 <jats:title>Abstract</jats:title><jats:p>Ninety‐four putative G protein‐coupled receptors (GPCRs) were identified in the <jats:italic>Musca domestica</jats:italic> genome. They were annotated and compared with their homologues in <jats:italic>Drosophila melanogaster</jats:italic>. Phylogenetic analyses of the GPCRs from both species revealed that several family members shared a closer relationship based on the domain architecture. The expression profiles of these genes were examined by quantitative real‐time PCR amongst three strains of the house fly, a near‐isogenic line strain with imidacloprid resistance (N‐IRS), the corresponding susceptible strain (CSS) and another strain derived from field populations with imidacloprid resistance (IRS). We found that five GPCR genes were upregulated in the N‐IRS and eight GPCR genes were upregulated in the IRS strains compared to the CSS strain. The transgenic lines of <jats:italic>D. melanogaster</jats:italic> with the GPCR genes (<jats:italic>LOC101899380</jats:italic> in the N‐IRS strain and <jats:italic>LOC101895664</jats:italic> in the IRS strain) exhibited significantly increased tolerance to imidacloprid, and higher expression of cytochrome P450 genes. Bioinformatic analysis of <jats:italic>LOC101899380</jats:italic> was carried out based on its full‐length nucleic acid sequence and putative amino acid sequence, and it was named Methuselah‐like10 (Mthl10) owing to its homology with <jats:italic>D. melanogaster</jats:italic> Mthl10. A cell‐base cell counting kit‐8 toxicity assay demonstrated that the expression of the GPCR gene <jats:italic>LOC101899380</jats:italic> in <jats:italic>Spodoptera frugiperda</jats:italic> (Sf9) cells using a baculovirus‐mediated expression system can elevate the cell tolerance to imidacloprid, indirectly supporting the hypothesis that the GPCR gene <jats:italic>LOC101899380</jats:italic> plays some role in imidacloprid resistance. These results should be useful for furthering understanding of the regulatory pathway by which house flies develop resistance.</jats:p> The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, <i>Musca domestica</i> (Diptera: Muscidae), to imidacloprid Insect Molecular Biology |
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| title |
The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid |
| title_unstemmed |
The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid |
| title_full |
The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid |
| title_fullStr |
The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid |
| title_full_unstemmed |
The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid |
| title_short |
The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid |
| title_sort |
the role of g protein‐coupled receptor‐related genes in cytochrome p450‐mediated resistance of the house fly, <i>musca domestica</i> (diptera: muscidae), to imidacloprid |
| topic |
Insect Science Genetics Molecular Biology |
| url |
http://dx.doi.org/10.1111/imb.12615 |
| publishDate |
2020 |
| physical |
92-103 |
| description |
<jats:title>Abstract</jats:title><jats:p>Ninety‐four putative G protein‐coupled receptors (GPCRs) were identified in the <jats:italic>Musca domestica</jats:italic> genome. They were annotated and compared with their homologues in <jats:italic>Drosophila melanogaster</jats:italic>. Phylogenetic analyses of the GPCRs from both species revealed that several family members shared a closer relationship based on the domain architecture. The expression profiles of these genes were examined by quantitative real‐time PCR amongst three strains of the house fly, a near‐isogenic line strain with imidacloprid resistance (N‐IRS), the corresponding susceptible strain (CSS) and another strain derived from field populations with imidacloprid resistance (IRS). We found that five GPCR genes were upregulated in the N‐IRS and eight GPCR genes were upregulated in the IRS strains compared to the CSS strain. The transgenic lines of <jats:italic>D. melanogaster</jats:italic> with the GPCR genes (<jats:italic>LOC101899380</jats:italic> in the N‐IRS strain and <jats:italic>LOC101895664</jats:italic> in the IRS strain) exhibited significantly increased tolerance to imidacloprid, and higher expression of cytochrome P450 genes. Bioinformatic analysis of <jats:italic>LOC101899380</jats:italic> was carried out based on its full‐length nucleic acid sequence and putative amino acid sequence, and it was named Methuselah‐like10 (Mthl10) owing to its homology with <jats:italic>D. melanogaster</jats:italic> Mthl10. A cell‐base cell counting kit‐8 toxicity assay demonstrated that the expression of the GPCR gene <jats:italic>LOC101899380</jats:italic> in <jats:italic>Spodoptera frugiperda</jats:italic> (Sf9) cells using a baculovirus‐mediated expression system can elevate the cell tolerance to imidacloprid, indirectly supporting the hypothesis that the GPCR gene <jats:italic>LOC101899380</jats:italic> plays some role in imidacloprid resistance. These results should be useful for furthering understanding of the regulatory pathway by which house flies develop resistance.</jats:p> |
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| author | Ma, Z., Zhang, Y., You, C., Zeng, X., Gao, X. |
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| description | <jats:title>Abstract</jats:title><jats:p>Ninety‐four putative G protein‐coupled receptors (GPCRs) were identified in the <jats:italic>Musca domestica</jats:italic> genome. They were annotated and compared with their homologues in <jats:italic>Drosophila melanogaster</jats:italic>. Phylogenetic analyses of the GPCRs from both species revealed that several family members shared a closer relationship based on the domain architecture. The expression profiles of these genes were examined by quantitative real‐time PCR amongst three strains of the house fly, a near‐isogenic line strain with imidacloprid resistance (N‐IRS), the corresponding susceptible strain (CSS) and another strain derived from field populations with imidacloprid resistance (IRS). We found that five GPCR genes were upregulated in the N‐IRS and eight GPCR genes were upregulated in the IRS strains compared to the CSS strain. The transgenic lines of <jats:italic>D. melanogaster</jats:italic> with the GPCR genes (<jats:italic>LOC101899380</jats:italic> in the N‐IRS strain and <jats:italic>LOC101895664</jats:italic> in the IRS strain) exhibited significantly increased tolerance to imidacloprid, and higher expression of cytochrome P450 genes. Bioinformatic analysis of <jats:italic>LOC101899380</jats:italic> was carried out based on its full‐length nucleic acid sequence and putative amino acid sequence, and it was named Methuselah‐like10 (Mthl10) owing to its homology with <jats:italic>D. melanogaster</jats:italic> Mthl10. A cell‐base cell counting kit‐8 toxicity assay demonstrated that the expression of the GPCR gene <jats:italic>LOC101899380</jats:italic> in <jats:italic>Spodoptera frugiperda</jats:italic> (Sf9) cells using a baculovirus‐mediated expression system can elevate the cell tolerance to imidacloprid, indirectly supporting the hypothesis that the GPCR gene <jats:italic>LOC101899380</jats:italic> plays some role in imidacloprid resistance. These results should be useful for furthering understanding of the regulatory pathway by which house flies develop resistance.</jats:p> |
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| institution | DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275 |
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| spelling | Ma, Z. Zhang, Y. You, C. Zeng, X. Gao, X. 0962-1075 1365-2583 Wiley Insect Science Genetics Molecular Biology http://dx.doi.org/10.1111/imb.12615 <jats:title>Abstract</jats:title><jats:p>Ninety‐four putative G protein‐coupled receptors (GPCRs) were identified in the <jats:italic>Musca domestica</jats:italic> genome. They were annotated and compared with their homologues in <jats:italic>Drosophila melanogaster</jats:italic>. Phylogenetic analyses of the GPCRs from both species revealed that several family members shared a closer relationship based on the domain architecture. The expression profiles of these genes were examined by quantitative real‐time PCR amongst three strains of the house fly, a near‐isogenic line strain with imidacloprid resistance (N‐IRS), the corresponding susceptible strain (CSS) and another strain derived from field populations with imidacloprid resistance (IRS). We found that five GPCR genes were upregulated in the N‐IRS and eight GPCR genes were upregulated in the IRS strains compared to the CSS strain. The transgenic lines of <jats:italic>D. melanogaster</jats:italic> with the GPCR genes (<jats:italic>LOC101899380</jats:italic> in the N‐IRS strain and <jats:italic>LOC101895664</jats:italic> in the IRS strain) exhibited significantly increased tolerance to imidacloprid, and higher expression of cytochrome P450 genes. Bioinformatic analysis of <jats:italic>LOC101899380</jats:italic> was carried out based on its full‐length nucleic acid sequence and putative amino acid sequence, and it was named Methuselah‐like10 (Mthl10) owing to its homology with <jats:italic>D. melanogaster</jats:italic> Mthl10. A cell‐base cell counting kit‐8 toxicity assay demonstrated that the expression of the GPCR gene <jats:italic>LOC101899380</jats:italic> in <jats:italic>Spodoptera frugiperda</jats:italic> (Sf9) cells using a baculovirus‐mediated expression system can elevate the cell tolerance to imidacloprid, indirectly supporting the hypothesis that the GPCR gene <jats:italic>LOC101899380</jats:italic> plays some role in imidacloprid resistance. These results should be useful for furthering understanding of the regulatory pathway by which house flies develop resistance.</jats:p> The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, <i>Musca domestica</i> (Diptera: Muscidae), to imidacloprid Insect Molecular Biology |
| spellingShingle | Ma, Z., Zhang, Y., You, C., Zeng, X., Gao, X., Insect Molecular Biology, The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid, Insect Science, Genetics, Molecular Biology |
| title | The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid |
| title_full | The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid |
| title_fullStr | The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid |
| title_full_unstemmed | The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid |
| title_short | The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid |
| title_sort | the role of g protein‐coupled receptor‐related genes in cytochrome p450‐mediated resistance of the house fly, <i>musca domestica</i> (diptera: muscidae), to imidacloprid |
| title_unstemmed | The role of G protein‐coupled receptor‐related genes in cytochrome P450‐mediated resistance of the house fly, Musca domestica (Diptera: Muscidae), to imidacloprid |
| topic | Insect Science, Genetics, Molecular Biology |
| url | http://dx.doi.org/10.1111/imb.12615 |