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Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus
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| Zeitschriftentitel: | Insect Molecular Biology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , |
| In: | Insect Molecular Biology, 28, 2019, 6, S. 862-872 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Dai, X. Li, R. Li, X. Liang, Y. Gao, Y. Xu, Y. Shi, L. Zhou, Y. Wang, H. Dai, X. Li, R. Li, X. Liang, Y. Gao, Y. Xu, Y. Shi, L. Zhou, Y. Wang, H. |
|---|---|
| author |
Dai, X. Li, R. Li, X. Liang, Y. Gao, Y. Xu, Y. Shi, L. Zhou, Y. Wang, H. |
| spellingShingle |
Dai, X. Li, R. Li, X. Liang, Y. Gao, Y. Xu, Y. Shi, L. Zhou, Y. Wang, H. Insect Molecular Biology Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus Insect Science Genetics Molecular Biology |
| author_sort |
dai, x. |
| spelling |
Dai, X. Li, R. Li, X. Liang, Y. Gao, Y. Xu, Y. Shi, L. Zhou, Y. Wang, H. 0962-1075 1365-2583 Wiley Insect Science Genetics Molecular Biology http://dx.doi.org/10.1111/imb.12603 <jats:title>Abstract</jats:title><jats:p>Sucrose is the main product of photosynthesis in plants, providing a rich carbon and energy source for the physiological growth and development of insects. In a previous study, we identified a novel sucrose hydrolase (SUH) in the larval midgut of moths. Intriguingly, there are two copies of <jats:italic>Suh</jats:italic>, namely <jats:italic>Suh1</jats:italic> and <jats:italic>Suh2</jats:italic>, in several species of butterflies. However, the biochemical characteristics of SUHs in butterflies remain unclear. In this study, we found that this duplication and subsequent diversification produced two <jats:italic>Suh</jats:italic> genes in <jats:italic>Papilio xuthus</jats:italic>. These two <jats:italic>PxSuh</jats:italic> genes were significantly divergent in terms of their expression pattern and enzyme properties. <jats:italic>PxSuh</jats:italic> messenger RNA expression was highest during the larval stage, reduced in the prepupal and pupal stages and, for <jats:italic>PxSuh1</jats:italic>, slightly increased again in the adult. The observed levels of <jats:italic>PxSuh2</jats:italic> were overall below those of <jats:italic>PxSuh1</jats:italic> amongst the development stages examined. Compared with PxSUH2, which has maintained the original gene function of maltose hydrolysis, PxSUH1 exhibits substrate specificity for sucrose with an optimum enzyme activity occurring at an alkaline pH. The data show that <jats:italic>PxSuh1</jats:italic> is evolutionarily adapted for effective functioning in an alkaline digestive system. Furthermore, we find that functional diversification of <jats:italic>Suh</jats:italic> facilitates <jats:italic>P. xuthus</jats:italic> to digestive carbohydrate of host plants. Thus, our findings offer new insights into the ecological and evolutionary adaptation of digestive enzymes in butterflies.</jats:p> Gene duplication and subsequent functional diversification of sucrose hydrolase in <i>Papilio xuthus</i> Insect Molecular Biology |
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| title |
Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus |
| title_unstemmed |
Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus |
| title_full |
Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus |
| title_fullStr |
Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus |
| title_full_unstemmed |
Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus |
| title_short |
Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus |
| title_sort |
gene duplication and subsequent functional diversification of sucrose hydrolase in <i>papilio xuthus</i> |
| topic |
Insect Science Genetics Molecular Biology |
| url |
http://dx.doi.org/10.1111/imb.12603 |
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2019 |
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862-872 |
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<jats:title>Abstract</jats:title><jats:p>Sucrose is the main product of photosynthesis in plants, providing a rich carbon and energy source for the physiological growth and development of insects. In a previous study, we identified a novel sucrose hydrolase (SUH) in the larval midgut of moths. Intriguingly, there are two copies of <jats:italic>Suh</jats:italic>, namely <jats:italic>Suh1</jats:italic> and <jats:italic>Suh2</jats:italic>, in several species of butterflies. However, the biochemical characteristics of SUHs in butterflies remain unclear. In this study, we found that this duplication and subsequent diversification produced two <jats:italic>Suh</jats:italic> genes in <jats:italic>Papilio xuthus</jats:italic>. These two <jats:italic>PxSuh</jats:italic> genes were significantly divergent in terms of their expression pattern and enzyme properties. <jats:italic>PxSuh</jats:italic> messenger RNA expression was highest during the larval stage, reduced in the prepupal and pupal stages and, for <jats:italic>PxSuh1</jats:italic>, slightly increased again in the adult. The observed levels of <jats:italic>PxSuh2</jats:italic> were overall below those of <jats:italic>PxSuh1</jats:italic> amongst the development stages examined. Compared with PxSUH2, which has maintained the original gene function of maltose hydrolysis, PxSUH1 exhibits substrate specificity for sucrose with an optimum enzyme activity occurring at an alkaline pH. The data show that <jats:italic>PxSuh1</jats:italic> is evolutionarily adapted for effective functioning in an alkaline digestive system. Furthermore, we find that functional diversification of <jats:italic>Suh</jats:italic> facilitates <jats:italic>P. xuthus</jats:italic> to digestive carbohydrate of host plants. Thus, our findings offer new insights into the ecological and evolutionary adaptation of digestive enzymes in butterflies.</jats:p> |
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| author | Dai, X., Li, R., Li, X., Liang, Y., Gao, Y., Xu, Y., Shi, L., Zhou, Y., Wang, H. |
| author_facet | Dai, X., Li, R., Li, X., Liang, Y., Gao, Y., Xu, Y., Shi, L., Zhou, Y., Wang, H., Dai, X., Li, R., Li, X., Liang, Y., Gao, Y., Xu, Y., Shi, L., Zhou, Y., Wang, H. |
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| description | <jats:title>Abstract</jats:title><jats:p>Sucrose is the main product of photosynthesis in plants, providing a rich carbon and energy source for the physiological growth and development of insects. In a previous study, we identified a novel sucrose hydrolase (SUH) in the larval midgut of moths. Intriguingly, there are two copies of <jats:italic>Suh</jats:italic>, namely <jats:italic>Suh1</jats:italic> and <jats:italic>Suh2</jats:italic>, in several species of butterflies. However, the biochemical characteristics of SUHs in butterflies remain unclear. In this study, we found that this duplication and subsequent diversification produced two <jats:italic>Suh</jats:italic> genes in <jats:italic>Papilio xuthus</jats:italic>. These two <jats:italic>PxSuh</jats:italic> genes were significantly divergent in terms of their expression pattern and enzyme properties. <jats:italic>PxSuh</jats:italic> messenger RNA expression was highest during the larval stage, reduced in the prepupal and pupal stages and, for <jats:italic>PxSuh1</jats:italic>, slightly increased again in the adult. The observed levels of <jats:italic>PxSuh2</jats:italic> were overall below those of <jats:italic>PxSuh1</jats:italic> amongst the development stages examined. Compared with PxSUH2, which has maintained the original gene function of maltose hydrolysis, PxSUH1 exhibits substrate specificity for sucrose with an optimum enzyme activity occurring at an alkaline pH. The data show that <jats:italic>PxSuh1</jats:italic> is evolutionarily adapted for effective functioning in an alkaline digestive system. Furthermore, we find that functional diversification of <jats:italic>Suh</jats:italic> facilitates <jats:italic>P. xuthus</jats:italic> to digestive carbohydrate of host plants. Thus, our findings offer new insights into the ecological and evolutionary adaptation of digestive enzymes in butterflies.</jats:p> |
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| institution | DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
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| series | Insect Molecular Biology |
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| spelling | Dai, X. Li, R. Li, X. Liang, Y. Gao, Y. Xu, Y. Shi, L. Zhou, Y. Wang, H. 0962-1075 1365-2583 Wiley Insect Science Genetics Molecular Biology http://dx.doi.org/10.1111/imb.12603 <jats:title>Abstract</jats:title><jats:p>Sucrose is the main product of photosynthesis in plants, providing a rich carbon and energy source for the physiological growth and development of insects. In a previous study, we identified a novel sucrose hydrolase (SUH) in the larval midgut of moths. Intriguingly, there are two copies of <jats:italic>Suh</jats:italic>, namely <jats:italic>Suh1</jats:italic> and <jats:italic>Suh2</jats:italic>, in several species of butterflies. However, the biochemical characteristics of SUHs in butterflies remain unclear. In this study, we found that this duplication and subsequent diversification produced two <jats:italic>Suh</jats:italic> genes in <jats:italic>Papilio xuthus</jats:italic>. These two <jats:italic>PxSuh</jats:italic> genes were significantly divergent in terms of their expression pattern and enzyme properties. <jats:italic>PxSuh</jats:italic> messenger RNA expression was highest during the larval stage, reduced in the prepupal and pupal stages and, for <jats:italic>PxSuh1</jats:italic>, slightly increased again in the adult. The observed levels of <jats:italic>PxSuh2</jats:italic> were overall below those of <jats:italic>PxSuh1</jats:italic> amongst the development stages examined. Compared with PxSUH2, which has maintained the original gene function of maltose hydrolysis, PxSUH1 exhibits substrate specificity for sucrose with an optimum enzyme activity occurring at an alkaline pH. The data show that <jats:italic>PxSuh1</jats:italic> is evolutionarily adapted for effective functioning in an alkaline digestive system. Furthermore, we find that functional diversification of <jats:italic>Suh</jats:italic> facilitates <jats:italic>P. xuthus</jats:italic> to digestive carbohydrate of host plants. Thus, our findings offer new insights into the ecological and evolutionary adaptation of digestive enzymes in butterflies.</jats:p> Gene duplication and subsequent functional diversification of sucrose hydrolase in <i>Papilio xuthus</i> Insect Molecular Biology |
| spellingShingle | Dai, X., Li, R., Li, X., Liang, Y., Gao, Y., Xu, Y., Shi, L., Zhou, Y., Wang, H., Insect Molecular Biology, Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus, Insect Science, Genetics, Molecular Biology |
| title | Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus |
| title_full | Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus |
| title_fullStr | Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus |
| title_full_unstemmed | Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus |
| title_short | Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus |
| title_sort | gene duplication and subsequent functional diversification of sucrose hydrolase in <i>papilio xuthus</i> |
| title_unstemmed | Gene duplication and subsequent functional diversification of sucrose hydrolase in Papilio xuthus |
| topic | Insect Science, Genetics, Molecular Biology |
| url | http://dx.doi.org/10.1111/imb.12603 |