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Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction
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| Zeitschriftentitel: | Immunology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Immunology, 153, 2018, 2, S. 214-224 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Kolenbrander, Anne Grewe, Bastian Nemazee, David Überla, Klaus Temchura, Vladimir Kolenbrander, Anne Grewe, Bastian Nemazee, David Überla, Klaus Temchura, Vladimir |
|---|---|
| author |
Kolenbrander, Anne Grewe, Bastian Nemazee, David Überla, Klaus Temchura, Vladimir |
| spellingShingle |
Kolenbrander, Anne Grewe, Bastian Nemazee, David Überla, Klaus Temchura, Vladimir Immunology Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction Immunology Immunology and Allergy |
| author_sort |
kolenbrander, anne |
| spelling |
Kolenbrander, Anne Grewe, Bastian Nemazee, David Überla, Klaus Temchura, Vladimir 0019-2805 1365-2567 Wiley Immunology Immunology and Allergy http://dx.doi.org/10.1111/imm.12834 <jats:title>Summary</jats:title><jats:p>The minimum requirements for <jats:italic>in vitro</jats:italic> modelling of natural <jats:styled-content style="fixed-case">CD</jats:styled-content>4<jats:sup>+</jats:sup> T‐cell differentiation into T follicular helper (<jats:styled-content style="fixed-case">Tfh</jats:styled-content>) cells are still under investigation. We co‐cultured wild‐type and T‐cell receptor (<jats:styled-content style="fixed-case">TCR</jats:styled-content>) transgenic <jats:styled-content style="fixed-case">CD</jats:styled-content>4<jats:sup>+</jats:sup> T cells from naive mice with dendritic cells and B‐cell receptor (<jats:styled-content style="fixed-case">BCR</jats:styled-content>) transgenic B cells in the presence of <jats:styled-content style="fixed-case">HIV</jats:styled-content>‐derived virus‐like particles containing matched B‐cell and T‐cell epitopes. This co‐culturing induced co‐expression of <jats:styled-content style="fixed-case">Tfh</jats:styled-content>‐master regulator transcription factor <jats:styled-content style="fixed-case">BCL</jats:styled-content>‐6 and <jats:styled-content style="fixed-case">CXCR</jats:styled-content>5 in up to 10% of the wild‐type and up to 40% of the <jats:styled-content style="fixed-case">TCR</jats:styled-content>‐transgenic <jats:styled-content style="fixed-case">CD</jats:styled-content>4<jats:sup>+</jats:sup> T cells. Phenotypic markers, production of interleukin‐21 and isotype switching of the B cells to IgG1 further indicated a helper function of the induced <jats:styled-content style="fixed-case">Tfh</jats:styled-content> cells <jats:italic>in vitro</jats:italic>. Dendritic cells supported the generation of functional <jats:styled-content style="fixed-case">Tfh</jats:styled-content> cells, but were unable to induce them without cognate B cells. Hence, our study presents a robust experimental system for efficient generation of functionally active <jats:styled-content style="fixed-case">Tfh</jats:styled-content> cells <jats:italic>in vitro</jats:italic> and confirms the importance of cognate B‐ and T‐cell cross‐talk for the <jats:styled-content style="fixed-case">Tfh</jats:styled-content> differentiation process.</jats:p> Generation of T follicular helper cells <i>in vitro</i>: requirement for <scp>B</scp>‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction Immunology |
| doi_str_mv |
10.1111/imm.12834 |
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Medizin |
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ElectronicArticle |
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| imprint |
Wiley, 2018 |
| imprint_str_mv |
Wiley, 2018 |
| issn |
0019-2805 1365-2567 |
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0019-2805 1365-2567 |
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kolenbrander2018generationoftfollicularhelpercellsinvitrorequirementforbcellreceptorcrosslinkingandcognatebandtcellinteraction |
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2018 |
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Immunology |
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49 |
| title |
Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction |
| title_unstemmed |
Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction |
| title_full |
Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction |
| title_fullStr |
Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction |
| title_full_unstemmed |
Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction |
| title_short |
Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction |
| title_sort |
generation of t follicular helper cells <i>in vitro</i>: requirement for <scp>b</scp>‐cell receptor cross‐linking and cognate b‐ and t‐cell interaction |
| topic |
Immunology Immunology and Allergy |
| url |
http://dx.doi.org/10.1111/imm.12834 |
| publishDate |
2018 |
| physical |
214-224 |
| description |
<jats:title>Summary</jats:title><jats:p>The minimum requirements for <jats:italic>in vitro</jats:italic> modelling of natural <jats:styled-content style="fixed-case">CD</jats:styled-content>4<jats:sup>+</jats:sup> T‐cell differentiation into T follicular helper (<jats:styled-content style="fixed-case">Tfh</jats:styled-content>) cells are still under investigation. We co‐cultured wild‐type and T‐cell receptor (<jats:styled-content style="fixed-case">TCR</jats:styled-content>) transgenic <jats:styled-content style="fixed-case">CD</jats:styled-content>4<jats:sup>+</jats:sup> T cells from naive mice with dendritic cells and B‐cell receptor (<jats:styled-content style="fixed-case">BCR</jats:styled-content>) transgenic B cells in the presence of <jats:styled-content style="fixed-case">HIV</jats:styled-content>‐derived virus‐like particles containing matched B‐cell and T‐cell epitopes. This co‐culturing induced co‐expression of <jats:styled-content style="fixed-case">Tfh</jats:styled-content>‐master regulator transcription factor <jats:styled-content style="fixed-case">BCL</jats:styled-content>‐6 and <jats:styled-content style="fixed-case">CXCR</jats:styled-content>5 in up to 10% of the wild‐type and up to 40% of the <jats:styled-content style="fixed-case">TCR</jats:styled-content>‐transgenic <jats:styled-content style="fixed-case">CD</jats:styled-content>4<jats:sup>+</jats:sup> T cells. Phenotypic markers, production of interleukin‐21 and isotype switching of the B cells to IgG1 further indicated a helper function of the induced <jats:styled-content style="fixed-case">Tfh</jats:styled-content> cells <jats:italic>in vitro</jats:italic>. Dendritic cells supported the generation of functional <jats:styled-content style="fixed-case">Tfh</jats:styled-content> cells, but were unable to induce them without cognate B cells. Hence, our study presents a robust experimental system for efficient generation of functionally active <jats:styled-content style="fixed-case">Tfh</jats:styled-content> cells <jats:italic>in vitro</jats:italic> and confirms the importance of cognate B‐ and T‐cell cross‐talk for the <jats:styled-content style="fixed-case">Tfh</jats:styled-content> differentiation process.</jats:p> |
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| author | Kolenbrander, Anne, Grewe, Bastian, Nemazee, David, Überla, Klaus, Temchura, Vladimir |
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| description | <jats:title>Summary</jats:title><jats:p>The minimum requirements for <jats:italic>in vitro</jats:italic> modelling of natural <jats:styled-content style="fixed-case">CD</jats:styled-content>4<jats:sup>+</jats:sup> T‐cell differentiation into T follicular helper (<jats:styled-content style="fixed-case">Tfh</jats:styled-content>) cells are still under investigation. We co‐cultured wild‐type and T‐cell receptor (<jats:styled-content style="fixed-case">TCR</jats:styled-content>) transgenic <jats:styled-content style="fixed-case">CD</jats:styled-content>4<jats:sup>+</jats:sup> T cells from naive mice with dendritic cells and B‐cell receptor (<jats:styled-content style="fixed-case">BCR</jats:styled-content>) transgenic B cells in the presence of <jats:styled-content style="fixed-case">HIV</jats:styled-content>‐derived virus‐like particles containing matched B‐cell and T‐cell epitopes. This co‐culturing induced co‐expression of <jats:styled-content style="fixed-case">Tfh</jats:styled-content>‐master regulator transcription factor <jats:styled-content style="fixed-case">BCL</jats:styled-content>‐6 and <jats:styled-content style="fixed-case">CXCR</jats:styled-content>5 in up to 10% of the wild‐type and up to 40% of the <jats:styled-content style="fixed-case">TCR</jats:styled-content>‐transgenic <jats:styled-content style="fixed-case">CD</jats:styled-content>4<jats:sup>+</jats:sup> T cells. Phenotypic markers, production of interleukin‐21 and isotype switching of the B cells to IgG1 further indicated a helper function of the induced <jats:styled-content style="fixed-case">Tfh</jats:styled-content> cells <jats:italic>in vitro</jats:italic>. Dendritic cells supported the generation of functional <jats:styled-content style="fixed-case">Tfh</jats:styled-content> cells, but were unable to induce them without cognate B cells. Hence, our study presents a robust experimental system for efficient generation of functionally active <jats:styled-content style="fixed-case">Tfh</jats:styled-content> cells <jats:italic>in vitro</jats:italic> and confirms the importance of cognate B‐ and T‐cell cross‐talk for the <jats:styled-content style="fixed-case">Tfh</jats:styled-content> differentiation process.</jats:p> |
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| imprint | Wiley, 2018 |
| imprint_str_mv | Wiley, 2018 |
| institution | DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
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| spelling | Kolenbrander, Anne Grewe, Bastian Nemazee, David Überla, Klaus Temchura, Vladimir 0019-2805 1365-2567 Wiley Immunology Immunology and Allergy http://dx.doi.org/10.1111/imm.12834 <jats:title>Summary</jats:title><jats:p>The minimum requirements for <jats:italic>in vitro</jats:italic> modelling of natural <jats:styled-content style="fixed-case">CD</jats:styled-content>4<jats:sup>+</jats:sup> T‐cell differentiation into T follicular helper (<jats:styled-content style="fixed-case">Tfh</jats:styled-content>) cells are still under investigation. We co‐cultured wild‐type and T‐cell receptor (<jats:styled-content style="fixed-case">TCR</jats:styled-content>) transgenic <jats:styled-content style="fixed-case">CD</jats:styled-content>4<jats:sup>+</jats:sup> T cells from naive mice with dendritic cells and B‐cell receptor (<jats:styled-content style="fixed-case">BCR</jats:styled-content>) transgenic B cells in the presence of <jats:styled-content style="fixed-case">HIV</jats:styled-content>‐derived virus‐like particles containing matched B‐cell and T‐cell epitopes. This co‐culturing induced co‐expression of <jats:styled-content style="fixed-case">Tfh</jats:styled-content>‐master regulator transcription factor <jats:styled-content style="fixed-case">BCL</jats:styled-content>‐6 and <jats:styled-content style="fixed-case">CXCR</jats:styled-content>5 in up to 10% of the wild‐type and up to 40% of the <jats:styled-content style="fixed-case">TCR</jats:styled-content>‐transgenic <jats:styled-content style="fixed-case">CD</jats:styled-content>4<jats:sup>+</jats:sup> T cells. Phenotypic markers, production of interleukin‐21 and isotype switching of the B cells to IgG1 further indicated a helper function of the induced <jats:styled-content style="fixed-case">Tfh</jats:styled-content> cells <jats:italic>in vitro</jats:italic>. Dendritic cells supported the generation of functional <jats:styled-content style="fixed-case">Tfh</jats:styled-content> cells, but were unable to induce them without cognate B cells. Hence, our study presents a robust experimental system for efficient generation of functionally active <jats:styled-content style="fixed-case">Tfh</jats:styled-content> cells <jats:italic>in vitro</jats:italic> and confirms the importance of cognate B‐ and T‐cell cross‐talk for the <jats:styled-content style="fixed-case">Tfh</jats:styled-content> differentiation process.</jats:p> Generation of T follicular helper cells <i>in vitro</i>: requirement for <scp>B</scp>‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction Immunology |
| spellingShingle | Kolenbrander, Anne, Grewe, Bastian, Nemazee, David, Überla, Klaus, Temchura, Vladimir, Immunology, Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction, Immunology, Immunology and Allergy |
| title | Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction |
| title_full | Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction |
| title_fullStr | Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction |
| title_full_unstemmed | Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction |
| title_short | Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction |
| title_sort | generation of t follicular helper cells <i>in vitro</i>: requirement for <scp>b</scp>‐cell receptor cross‐linking and cognate b‐ and t‐cell interaction |
| title_unstemmed | Generation of T follicular helper cells in vitro: requirement for B‐cell receptor cross‐linking and cognate B‐ and T‐cell interaction |
| topic | Immunology, Immunology and Allergy |
| url | http://dx.doi.org/10.1111/imm.12834 |