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MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke
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| Zeitschriftentitel: | CNS Neuroscience & Therapeutics |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | CNS Neuroscience & Therapeutics, 19, 2013, 10, S. 813-819 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Sun, Yang Gui, Huan Li, Qi Luo, Zhu‐Min Zheng, Min‐Jun Duan, Jun‐Li Liu, Xia Sun, Yang Gui, Huan Li, Qi Luo, Zhu‐Min Zheng, Min‐Jun Duan, Jun‐Li Liu, Xia |
|---|---|
| author |
Sun, Yang Gui, Huan Li, Qi Luo, Zhu‐Min Zheng, Min‐Jun Duan, Jun‐Li Liu, Xia |
| spellingShingle |
Sun, Yang Gui, Huan Li, Qi Luo, Zhu‐Min Zheng, Min‐Jun Duan, Jun‐Li Liu, Xia CNS Neuroscience & Therapeutics MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke Pharmacology (medical) Physiology (medical) Psychiatry and Mental health Pharmacology |
| author_sort |
sun, yang |
| spelling |
Sun, Yang Gui, Huan Li, Qi Luo, Zhu‐Min Zheng, Min‐Jun Duan, Jun‐Li Liu, Xia 1755-5930 1755-5949 Wiley Pharmacology (medical) Physiology (medical) Psychiatry and Mental health Pharmacology http://dx.doi.org/10.1111/cns.12142 <jats:title>Summary</jats:title><jats:sec><jats:title>Aims</jats:title><jats:p>To explore the role and underlying mechanism of miR‐124 in stroke.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>miR‐124 expression was determined by real‐time PCR. The effect of miR‐124 on infarct area was assessed in middle cerebral artery occlusion (MCAO) mice. The influence of miR‐124 on oxygen and glucose deprivation (OGD) induced neuron apoptosis and death was examined by immunofluorescence. The effect of miR‐124 on apoptosis‐related proteins was determined by Western blot.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The level of miR‐124 is significantly increased in ischemic penumbra as compared with that in nonischemic area of MACO mice. Brain tissue of stroke‐prone spontaneously hypertensive rats (SHR‐SP) also showed higher level of miR‐124 as compared with that of spontaneously hypertensive rats (SHR). Consistently, OGD treatment obviously increased miR‐124 level in primary neurons. <jats:italic>In vivo</jats:italic>, miR‐124 overexpression significantly decreased, while miR‐124 knockdown significantly increased, the infarct area of MCAO mice. <jats:italic>In vitro</jats:italic>, gain or loss of miR‐124 function resulted in reduced or increased neuron apoptosis and death induced by OGD, and increased or reduced antiapoptosis protein, Bcl‐2 and Bcl‐xl, respectively.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>miR‐124 plays a neurons‐protective role <jats:italic>via</jats:italic> apoptosis‐inhibiting pathway in ischemic stroke.</jats:p></jats:sec> MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke CNS Neuroscience & Therapeutics |
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10.1111/cns.12142 |
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Online Free |
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Chemie und Pharmazie Biologie Medizin Psychologie |
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Wiley, 2013 |
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Wiley, 2013 |
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1755-5930 1755-5949 |
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2013 |
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CNS Neuroscience & Therapeutics |
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49 |
| title |
MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke |
| title_unstemmed |
MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke |
| title_full |
MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke |
| title_fullStr |
MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke |
| title_full_unstemmed |
MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke |
| title_short |
MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke |
| title_sort |
microrna‐124 protects neurons against apoptosis in cerebral ischemic stroke |
| topic |
Pharmacology (medical) Physiology (medical) Psychiatry and Mental health Pharmacology |
| url |
http://dx.doi.org/10.1111/cns.12142 |
| publishDate |
2013 |
| physical |
813-819 |
| description |
<jats:title>Summary</jats:title><jats:sec><jats:title>Aims</jats:title><jats:p>To explore the role and underlying mechanism of miR‐124 in stroke.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>miR‐124 expression was determined by real‐time PCR. The effect of miR‐124 on infarct area was assessed in middle cerebral artery occlusion (MCAO) mice. The influence of miR‐124 on oxygen and glucose deprivation (OGD) induced neuron apoptosis and death was examined by immunofluorescence. The effect of miR‐124 on apoptosis‐related proteins was determined by Western blot.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The level of miR‐124 is significantly increased in ischemic penumbra as compared with that in nonischemic area of MACO mice. Brain tissue of stroke‐prone spontaneously hypertensive rats (SHR‐SP) also showed higher level of miR‐124 as compared with that of spontaneously hypertensive rats (SHR). Consistently, OGD treatment obviously increased miR‐124 level in primary neurons. <jats:italic>In vivo</jats:italic>, miR‐124 overexpression significantly decreased, while miR‐124 knockdown significantly increased, the infarct area of MCAO mice. <jats:italic>In vitro</jats:italic>, gain or loss of miR‐124 function resulted in reduced or increased neuron apoptosis and death induced by OGD, and increased or reduced antiapoptosis protein, Bcl‐2 and Bcl‐xl, respectively.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>miR‐124 plays a neurons‐protective role <jats:italic>via</jats:italic> apoptosis‐inhibiting pathway in ischemic stroke.</jats:p></jats:sec> |
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| author | Sun, Yang, Gui, Huan, Li, Qi, Luo, Zhu‐Min, Zheng, Min‐Jun, Duan, Jun‐Li, Liu, Xia |
| author_facet | Sun, Yang, Gui, Huan, Li, Qi, Luo, Zhu‐Min, Zheng, Min‐Jun, Duan, Jun‐Li, Liu, Xia, Sun, Yang, Gui, Huan, Li, Qi, Luo, Zhu‐Min, Zheng, Min‐Jun, Duan, Jun‐Li, Liu, Xia |
| author_sort | sun, yang |
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| description | <jats:title>Summary</jats:title><jats:sec><jats:title>Aims</jats:title><jats:p>To explore the role and underlying mechanism of miR‐124 in stroke.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>miR‐124 expression was determined by real‐time PCR. The effect of miR‐124 on infarct area was assessed in middle cerebral artery occlusion (MCAO) mice. The influence of miR‐124 on oxygen and glucose deprivation (OGD) induced neuron apoptosis and death was examined by immunofluorescence. The effect of miR‐124 on apoptosis‐related proteins was determined by Western blot.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The level of miR‐124 is significantly increased in ischemic penumbra as compared with that in nonischemic area of MACO mice. Brain tissue of stroke‐prone spontaneously hypertensive rats (SHR‐SP) also showed higher level of miR‐124 as compared with that of spontaneously hypertensive rats (SHR). Consistently, OGD treatment obviously increased miR‐124 level in primary neurons. <jats:italic>In vivo</jats:italic>, miR‐124 overexpression significantly decreased, while miR‐124 knockdown significantly increased, the infarct area of MCAO mice. <jats:italic>In vitro</jats:italic>, gain or loss of miR‐124 function resulted in reduced or increased neuron apoptosis and death induced by OGD, and increased or reduced antiapoptosis protein, Bcl‐2 and Bcl‐xl, respectively.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>miR‐124 plays a neurons‐protective role <jats:italic>via</jats:italic> apoptosis‐inhibiting pathway in ischemic stroke.</jats:p></jats:sec> |
| doi_str_mv | 10.1111/cns.12142 |
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| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTExMS9jbnMuMTIxNDI |
| imprint | Wiley, 2013 |
| imprint_str_mv | Wiley, 2013 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 1755-5930, 1755-5949 |
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| publishDateSort | 2013 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | CNS Neuroscience & Therapeutics |
| source_id | 49 |
| spelling | Sun, Yang Gui, Huan Li, Qi Luo, Zhu‐Min Zheng, Min‐Jun Duan, Jun‐Li Liu, Xia 1755-5930 1755-5949 Wiley Pharmacology (medical) Physiology (medical) Psychiatry and Mental health Pharmacology http://dx.doi.org/10.1111/cns.12142 <jats:title>Summary</jats:title><jats:sec><jats:title>Aims</jats:title><jats:p>To explore the role and underlying mechanism of miR‐124 in stroke.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>miR‐124 expression was determined by real‐time PCR. The effect of miR‐124 on infarct area was assessed in middle cerebral artery occlusion (MCAO) mice. The influence of miR‐124 on oxygen and glucose deprivation (OGD) induced neuron apoptosis and death was examined by immunofluorescence. The effect of miR‐124 on apoptosis‐related proteins was determined by Western blot.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The level of miR‐124 is significantly increased in ischemic penumbra as compared with that in nonischemic area of MACO mice. Brain tissue of stroke‐prone spontaneously hypertensive rats (SHR‐SP) also showed higher level of miR‐124 as compared with that of spontaneously hypertensive rats (SHR). Consistently, OGD treatment obviously increased miR‐124 level in primary neurons. <jats:italic>In vivo</jats:italic>, miR‐124 overexpression significantly decreased, while miR‐124 knockdown significantly increased, the infarct area of MCAO mice. <jats:italic>In vitro</jats:italic>, gain or loss of miR‐124 function resulted in reduced or increased neuron apoptosis and death induced by OGD, and increased or reduced antiapoptosis protein, Bcl‐2 and Bcl‐xl, respectively.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>miR‐124 plays a neurons‐protective role <jats:italic>via</jats:italic> apoptosis‐inhibiting pathway in ischemic stroke.</jats:p></jats:sec> MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke CNS Neuroscience & Therapeutics |
| spellingShingle | Sun, Yang, Gui, Huan, Li, Qi, Luo, Zhu‐Min, Zheng, Min‐Jun, Duan, Jun‐Li, Liu, Xia, CNS Neuroscience & Therapeutics, MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke, Pharmacology (medical), Physiology (medical), Psychiatry and Mental health, Pharmacology |
| title | MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke |
| title_full | MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke |
| title_fullStr | MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke |
| title_full_unstemmed | MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke |
| title_short | MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke |
| title_sort | microrna‐124 protects neurons against apoptosis in cerebral ischemic stroke |
| title_unstemmed | MicroRNA‐124 Protects Neurons Against Apoptosis in Cerebral Ischemic Stroke |
| topic | Pharmacology (medical), Physiology (medical), Psychiatry and Mental health, Pharmacology |
| url | http://dx.doi.org/10.1111/cns.12142 |