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Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1
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| Zeitschriftentitel: | Animal Genetics |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Animal Genetics, 50, 2019, 2, S. 157-161 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Liang, J. Zhang, Y. Wang, L. Liu, X. Yan, H. Wang, L. Zhang, L. Liang, J. Zhang, Y. Wang, L. Liu, X. Yan, H. Wang, L. Zhang, L. |
|---|---|
| author |
Liang, J. Zhang, Y. Wang, L. Liu, X. Yan, H. Wang, L. Zhang, L. |
| spellingShingle |
Liang, J. Zhang, Y. Wang, L. Liu, X. Yan, H. Wang, L. Zhang, L. Animal Genetics Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1 Genetics Animal Science and Zoology General Medicine |
| author_sort |
liang, j. |
| spelling |
Liang, J. Zhang, Y. Wang, L. Liu, X. Yan, H. Wang, L. Zhang, L. 0268-9146 1365-2052 Wiley Genetics Animal Science and Zoology General Medicine http://dx.doi.org/10.1111/age.12759 <jats:title>Summary</jats:title><jats:p>Considerable diversity exists in porcine ear size, which is an important morphological feature of pig breeds. Previously, we localized four crucial candidate genes—<jats:italic>high mobility group <jats:styled-content style="fixed-case">AT</jats:styled-content>‐hook 2</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">HMGA</jats:styled-content>2</jats:italic>), <jats:italic><jats:styled-content style="fixed-case">LEM</jats:styled-content> domain‐containing 3</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">LEMD</jats:styled-content>3</jats:italic>), <jats:italic>methionine sulfoxide reductase B3</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">MSRB</jats:styled-content>3</jats:italic>) and <jats:italic>Wnt inhibitory factor 1</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1</jats:italic>)—on <jats:italic>Sus Scrofa</jats:italic> chromosome 5 affecting porcine ear size, then cloned <jats:italic><jats:styled-content style="fixed-case">LEMD</jats:styled-content>3</jats:italic> and <jats:italic><jats:styled-content style="fixed-case">MSBR</jats:styled-content>3</jats:italic>. In this study, we performed rapid amplification of <jats:styled-content style="fixed-case">cDNA</jats:styled-content> ends to obtain full‐length <jats:styled-content style="fixed-case">cDNA</jats:styled-content> sequences of 2338‐bp <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1</jats:italic> and 2998‐bp <jats:italic><jats:styled-content style="fixed-case">HMGA</jats:styled-content>2</jats:italic>. Using quantitative real‐time <jats:styled-content style="fixed-case">PCR</jats:styled-content>, we revealed that <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1</jats:italic> expression was highest in ear cartilage of 60‐day‐old pigs and that this is therefore a better candidate gene for ear size than <jats:italic><jats:styled-content style="fixed-case">HMGA</jats:styled-content>2</jats:italic>. We further screened coding sequence variants in both genes and identified only one missense mutation (<jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1:</jats:italic>c.1167C>G) in a conserved epidermal growth factor‐like domain from the mammalian <jats:styled-content style="fixed-case">WIF</jats:styled-content>1 protein. The protein‐altering mutation was significantly associated with ear size across the Large White × Minzhu hybrid and Beijing Black pig populations. When <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1:</jats:italic>c.1167C>G was included as fixed effect in the model to re‐run a genome‐wide association study in the Large White × Minzhu intercross population the <jats:italic>P</jats:italic>‐value of the peak <jats:styled-content style="fixed-case">SNP</jats:styled-content> on <jats:styled-content style="fixed-case">SSC</jats:styled-content>5 from re‐running the genome‐wide association study dropped from 2.45E‐12 to 7.33E‐05. Taken together, the <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1:</jats:italic>c.1167C>G could be an important mutation associated with ear size. Our findings provide helpful information for further studies of the molecular mechanisms controlling porcine ear size.</jats:p> Molecular cloning of <i><scp>WIF</scp>1</i> and <i><scp>HMGA</scp>2</i> reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in <i><scp>WIF</scp>1</i> Animal Genetics |
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10.1111/age.12759 |
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| imprint |
Wiley, 2019 |
| imprint_str_mv |
Wiley, 2019 |
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0268-9146 1365-2052 |
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English |
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liang2019molecularcloningofwif1andhmga2revealsearpreferentialexpressionwhileuncoveringamissensemutationassociatedwithporcineearsizeinwif1 |
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2019 |
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Wiley |
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Animal Genetics |
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| title |
Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1 |
| title_unstemmed |
Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1 |
| title_full |
Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1 |
| title_fullStr |
Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1 |
| title_full_unstemmed |
Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1 |
| title_short |
Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1 |
| title_sort |
molecular cloning of <i><scp>wif</scp>1</i> and <i><scp>hmga</scp>2</i> reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in <i><scp>wif</scp>1</i> |
| topic |
Genetics Animal Science and Zoology General Medicine |
| url |
http://dx.doi.org/10.1111/age.12759 |
| publishDate |
2019 |
| physical |
157-161 |
| description |
<jats:title>Summary</jats:title><jats:p>Considerable diversity exists in porcine ear size, which is an important morphological feature of pig breeds. Previously, we localized four crucial candidate genes—<jats:italic>high mobility group <jats:styled-content style="fixed-case">AT</jats:styled-content>‐hook 2</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">HMGA</jats:styled-content>2</jats:italic>), <jats:italic><jats:styled-content style="fixed-case">LEM</jats:styled-content> domain‐containing 3</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">LEMD</jats:styled-content>3</jats:italic>), <jats:italic>methionine sulfoxide reductase B3</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">MSRB</jats:styled-content>3</jats:italic>) and <jats:italic>Wnt inhibitory factor 1</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1</jats:italic>)—on <jats:italic>Sus Scrofa</jats:italic> chromosome 5 affecting porcine ear size, then cloned <jats:italic><jats:styled-content style="fixed-case">LEMD</jats:styled-content>3</jats:italic> and <jats:italic><jats:styled-content style="fixed-case">MSBR</jats:styled-content>3</jats:italic>. In this study, we performed rapid amplification of <jats:styled-content style="fixed-case">cDNA</jats:styled-content> ends to obtain full‐length <jats:styled-content style="fixed-case">cDNA</jats:styled-content> sequences of 2338‐bp <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1</jats:italic> and 2998‐bp <jats:italic><jats:styled-content style="fixed-case">HMGA</jats:styled-content>2</jats:italic>. Using quantitative real‐time <jats:styled-content style="fixed-case">PCR</jats:styled-content>, we revealed that <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1</jats:italic> expression was highest in ear cartilage of 60‐day‐old pigs and that this is therefore a better candidate gene for ear size than <jats:italic><jats:styled-content style="fixed-case">HMGA</jats:styled-content>2</jats:italic>. We further screened coding sequence variants in both genes and identified only one missense mutation (<jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1:</jats:italic>c.1167C>G) in a conserved epidermal growth factor‐like domain from the mammalian <jats:styled-content style="fixed-case">WIF</jats:styled-content>1 protein. The protein‐altering mutation was significantly associated with ear size across the Large White × Minzhu hybrid and Beijing Black pig populations. When <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1:</jats:italic>c.1167C>G was included as fixed effect in the model to re‐run a genome‐wide association study in the Large White × Minzhu intercross population the <jats:italic>P</jats:italic>‐value of the peak <jats:styled-content style="fixed-case">SNP</jats:styled-content> on <jats:styled-content style="fixed-case">SSC</jats:styled-content>5 from re‐running the genome‐wide association study dropped from 2.45E‐12 to 7.33E‐05. Taken together, the <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1:</jats:italic>c.1167C>G could be an important mutation associated with ear size. Our findings provide helpful information for further studies of the molecular mechanisms controlling porcine ear size.</jats:p> |
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| description | <jats:title>Summary</jats:title><jats:p>Considerable diversity exists in porcine ear size, which is an important morphological feature of pig breeds. Previously, we localized four crucial candidate genes—<jats:italic>high mobility group <jats:styled-content style="fixed-case">AT</jats:styled-content>‐hook 2</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">HMGA</jats:styled-content>2</jats:italic>), <jats:italic><jats:styled-content style="fixed-case">LEM</jats:styled-content> domain‐containing 3</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">LEMD</jats:styled-content>3</jats:italic>), <jats:italic>methionine sulfoxide reductase B3</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">MSRB</jats:styled-content>3</jats:italic>) and <jats:italic>Wnt inhibitory factor 1</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1</jats:italic>)—on <jats:italic>Sus Scrofa</jats:italic> chromosome 5 affecting porcine ear size, then cloned <jats:italic><jats:styled-content style="fixed-case">LEMD</jats:styled-content>3</jats:italic> and <jats:italic><jats:styled-content style="fixed-case">MSBR</jats:styled-content>3</jats:italic>. In this study, we performed rapid amplification of <jats:styled-content style="fixed-case">cDNA</jats:styled-content> ends to obtain full‐length <jats:styled-content style="fixed-case">cDNA</jats:styled-content> sequences of 2338‐bp <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1</jats:italic> and 2998‐bp <jats:italic><jats:styled-content style="fixed-case">HMGA</jats:styled-content>2</jats:italic>. Using quantitative real‐time <jats:styled-content style="fixed-case">PCR</jats:styled-content>, we revealed that <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1</jats:italic> expression was highest in ear cartilage of 60‐day‐old pigs and that this is therefore a better candidate gene for ear size than <jats:italic><jats:styled-content style="fixed-case">HMGA</jats:styled-content>2</jats:italic>. We further screened coding sequence variants in both genes and identified only one missense mutation (<jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1:</jats:italic>c.1167C>G) in a conserved epidermal growth factor‐like domain from the mammalian <jats:styled-content style="fixed-case">WIF</jats:styled-content>1 protein. The protein‐altering mutation was significantly associated with ear size across the Large White × Minzhu hybrid and Beijing Black pig populations. When <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1:</jats:italic>c.1167C>G was included as fixed effect in the model to re‐run a genome‐wide association study in the Large White × Minzhu intercross population the <jats:italic>P</jats:italic>‐value of the peak <jats:styled-content style="fixed-case">SNP</jats:styled-content> on <jats:styled-content style="fixed-case">SSC</jats:styled-content>5 from re‐running the genome‐wide association study dropped from 2.45E‐12 to 7.33E‐05. Taken together, the <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1:</jats:italic>c.1167C>G could be an important mutation associated with ear size. Our findings provide helpful information for further studies of the molecular mechanisms controlling porcine ear size.</jats:p> |
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| imprint | Wiley, 2019 |
| imprint_str_mv | Wiley, 2019 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229 |
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| issn_str_mv | 0268-9146, 1365-2052 |
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| physical | 157-161 |
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| spelling | Liang, J. Zhang, Y. Wang, L. Liu, X. Yan, H. Wang, L. Zhang, L. 0268-9146 1365-2052 Wiley Genetics Animal Science and Zoology General Medicine http://dx.doi.org/10.1111/age.12759 <jats:title>Summary</jats:title><jats:p>Considerable diversity exists in porcine ear size, which is an important morphological feature of pig breeds. Previously, we localized four crucial candidate genes—<jats:italic>high mobility group <jats:styled-content style="fixed-case">AT</jats:styled-content>‐hook 2</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">HMGA</jats:styled-content>2</jats:italic>), <jats:italic><jats:styled-content style="fixed-case">LEM</jats:styled-content> domain‐containing 3</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">LEMD</jats:styled-content>3</jats:italic>), <jats:italic>methionine sulfoxide reductase B3</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">MSRB</jats:styled-content>3</jats:italic>) and <jats:italic>Wnt inhibitory factor 1</jats:italic> (<jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1</jats:italic>)—on <jats:italic>Sus Scrofa</jats:italic> chromosome 5 affecting porcine ear size, then cloned <jats:italic><jats:styled-content style="fixed-case">LEMD</jats:styled-content>3</jats:italic> and <jats:italic><jats:styled-content style="fixed-case">MSBR</jats:styled-content>3</jats:italic>. In this study, we performed rapid amplification of <jats:styled-content style="fixed-case">cDNA</jats:styled-content> ends to obtain full‐length <jats:styled-content style="fixed-case">cDNA</jats:styled-content> sequences of 2338‐bp <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1</jats:italic> and 2998‐bp <jats:italic><jats:styled-content style="fixed-case">HMGA</jats:styled-content>2</jats:italic>. Using quantitative real‐time <jats:styled-content style="fixed-case">PCR</jats:styled-content>, we revealed that <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1</jats:italic> expression was highest in ear cartilage of 60‐day‐old pigs and that this is therefore a better candidate gene for ear size than <jats:italic><jats:styled-content style="fixed-case">HMGA</jats:styled-content>2</jats:italic>. We further screened coding sequence variants in both genes and identified only one missense mutation (<jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1:</jats:italic>c.1167C>G) in a conserved epidermal growth factor‐like domain from the mammalian <jats:styled-content style="fixed-case">WIF</jats:styled-content>1 protein. The protein‐altering mutation was significantly associated with ear size across the Large White × Minzhu hybrid and Beijing Black pig populations. When <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1:</jats:italic>c.1167C>G was included as fixed effect in the model to re‐run a genome‐wide association study in the Large White × Minzhu intercross population the <jats:italic>P</jats:italic>‐value of the peak <jats:styled-content style="fixed-case">SNP</jats:styled-content> on <jats:styled-content style="fixed-case">SSC</jats:styled-content>5 from re‐running the genome‐wide association study dropped from 2.45E‐12 to 7.33E‐05. Taken together, the <jats:italic><jats:styled-content style="fixed-case">WIF</jats:styled-content>1:</jats:italic>c.1167C>G could be an important mutation associated with ear size. Our findings provide helpful information for further studies of the molecular mechanisms controlling porcine ear size.</jats:p> Molecular cloning of <i><scp>WIF</scp>1</i> and <i><scp>HMGA</scp>2</i> reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in <i><scp>WIF</scp>1</i> Animal Genetics |
| spellingShingle | Liang, J., Zhang, Y., Wang, L., Liu, X., Yan, H., Wang, L., Zhang, L., Animal Genetics, Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1, Genetics, Animal Science and Zoology, General Medicine |
| title | Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1 |
| title_full | Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1 |
| title_fullStr | Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1 |
| title_full_unstemmed | Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1 |
| title_short | Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1 |
| title_sort | molecular cloning of <i><scp>wif</scp>1</i> and <i><scp>hmga</scp>2</i> reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in <i><scp>wif</scp>1</i> |
| title_unstemmed | Molecular cloning of WIF1 and HMGA2 reveals ear‐preferential expression while uncovering a missense mutation associated with porcine ear size in WIF1 |
| topic | Genetics, Animal Science and Zoology, General Medicine |
| url | http://dx.doi.org/10.1111/age.12759 |