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Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing
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| Zeitschriftentitel: | Vox Sanguinis |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Vox Sanguinis, 112, 2017, 4, S. 360-366 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Hong, X. Chen, S. Ying, Y. Liu, Y. Xu, X. He, J. Zhu, F. Hong, X. Chen, S. Ying, Y. Liu, Y. Xu, X. He, J. Zhu, F. |
|---|---|
| author |
Hong, X. Chen, S. Ying, Y. Liu, Y. Xu, X. He, J. Zhu, F. |
| spellingShingle |
Hong, X. Chen, S. Ying, Y. Liu, Y. Xu, X. He, J. Zhu, F. Vox Sanguinis Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing Hematology General Medicine |
| author_sort |
hong, x. |
| spelling |
Hong, X. Chen, S. Ying, Y. Liu, Y. Xu, X. He, J. Zhu, F. 0042-9007 1423-0410 Wiley Hematology General Medicine http://dx.doi.org/10.1111/vox.12507 <jats:sec><jats:title>Background and Objectives</jats:title><jats:p>Human platelet alloantigen (<jats:styled-content style="fixed-case">HPA</jats:styled-content>) genotyping is important for the diagnosis and prevention the alloimmune platelet disorders. In this study, a simultaneous genotyping method for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to ‐28bw systems was established using multiplex <jats:styled-content style="fixed-case">PCR</jats:styled-content>‐<jats:styled-content style="fixed-case">SBT</jats:styled-content> and the frequencies of genotypes and alleles of <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to ‐28bw systems in the Zhejiang Han population were analysed.</jats:p></jats:sec><jats:sec><jats:title>Materials and Methods</jats:title><jats:p>The specific primers were designed according to the nucleotide sequences of <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to 28bw systems which are located in <jats:italic><jats:styled-content style="fixed-case">ITGB</jats:styled-content>3, <jats:styled-content style="fixed-case">GP</jats:styled-content>1<jats:styled-content style="fixed-case">BA</jats:styled-content>,<jats:styled-content style="fixed-case"> ITGA</jats:styled-content>2B, <jats:styled-content style="fixed-case">ITGA</jats:styled-content>2, <jats:styled-content style="fixed-case">GP</jats:styled-content>1<jats:styled-content style="fixed-case">BB</jats:styled-content></jats:italic> and <jats:italic><jats:styled-content style="fixed-case">CD</jats:styled-content>109,</jats:italic> respectively. The multiplex <jats:styled-content style="fixed-case">PCR</jats:styled-content> amplification systems were used, and then, the amplicons were purified and sequenced. A total of 335 healthy volunteer blood donors were detected.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The genotypes of ten reference samples from Platelet Immunology Workshop of <jats:styled-content style="fixed-case">ISBT</jats:styled-content> were in concordance with the known genotypes. Among the 28 <jats:styled-content style="fixed-case">HPA</jats:styled-content> systems, <jats:styled-content style="fixed-case">HPA</jats:styled-content> a and b alleles were found in <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to 6w, <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐15 and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐21w systems in the Chinese Han population, while only <jats:styled-content style="fixed-case">HPA</jats:styled-content> aa genotype was detected in the other <jats:styled-content style="fixed-case">HPA</jats:styled-content> systems. The frequencies of <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1b were 0·993 and 0·007, with 0·943 and 0·057 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐2a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐2b, 0·527 and 0·473 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐3a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐3b, 0·997 and 0·003 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐4a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐4b, 0·991 and 0·009 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐5a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐5b, 0·980 and 0·020 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐6wa and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐6wb, 0·508 and 0·492 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐15a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐15b and 0·994 and 0·006 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐21wa and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐21wb.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>One multiplex <jats:styled-content style="fixed-case">PCR</jats:styled-content>‐<jats:styled-content style="fixed-case">SBT</jats:styled-content> method for <jats:styled-content style="fixed-case">HPA</jats:styled-content>s was established and the data of the study could help to prevent and treat for alloimmune thrombocytopenia.</jats:p></jats:sec> Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing Vox Sanguinis |
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10.1111/vox.12507 |
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Online |
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| imprint |
Wiley, 2017 |
| imprint_str_mv |
Wiley, 2017 |
| issn |
0042-9007 1423-0410 |
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0042-9007 1423-0410 |
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English |
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hong2017simultaneousgenotypingofhumanplateletalloantigen1to28bwsystemsbymultiplexpolymerasechainreactionsequencebasedtyping |
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2017 |
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Wiley |
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Vox Sanguinis |
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49 |
| title |
Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| title_unstemmed |
Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| title_full |
Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| title_fullStr |
Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| title_full_unstemmed |
Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| title_short |
Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| title_sort |
simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| topic |
Hematology General Medicine |
| url |
http://dx.doi.org/10.1111/vox.12507 |
| publishDate |
2017 |
| physical |
360-366 |
| description |
<jats:sec><jats:title>Background and Objectives</jats:title><jats:p>Human platelet alloantigen (<jats:styled-content style="fixed-case">HPA</jats:styled-content>) genotyping is important for the diagnosis and prevention the alloimmune platelet disorders. In this study, a simultaneous genotyping method for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to ‐28bw systems was established using multiplex <jats:styled-content style="fixed-case">PCR</jats:styled-content>‐<jats:styled-content style="fixed-case">SBT</jats:styled-content> and the frequencies of genotypes and alleles of <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to ‐28bw systems in the Zhejiang Han population were analysed.</jats:p></jats:sec><jats:sec><jats:title>Materials and Methods</jats:title><jats:p>The specific primers were designed according to the nucleotide sequences of <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to 28bw systems which are located in <jats:italic><jats:styled-content style="fixed-case">ITGB</jats:styled-content>3, <jats:styled-content style="fixed-case">GP</jats:styled-content>1<jats:styled-content style="fixed-case">BA</jats:styled-content>,<jats:styled-content style="fixed-case"> ITGA</jats:styled-content>2B, <jats:styled-content style="fixed-case">ITGA</jats:styled-content>2, <jats:styled-content style="fixed-case">GP</jats:styled-content>1<jats:styled-content style="fixed-case">BB</jats:styled-content></jats:italic> and <jats:italic><jats:styled-content style="fixed-case">CD</jats:styled-content>109,</jats:italic> respectively. The multiplex <jats:styled-content style="fixed-case">PCR</jats:styled-content> amplification systems were used, and then, the amplicons were purified and sequenced. A total of 335 healthy volunteer blood donors were detected.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The genotypes of ten reference samples from Platelet Immunology Workshop of <jats:styled-content style="fixed-case">ISBT</jats:styled-content> were in concordance with the known genotypes. Among the 28 <jats:styled-content style="fixed-case">HPA</jats:styled-content> systems, <jats:styled-content style="fixed-case">HPA</jats:styled-content> a and b alleles were found in <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to 6w, <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐15 and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐21w systems in the Chinese Han population, while only <jats:styled-content style="fixed-case">HPA</jats:styled-content> aa genotype was detected in the other <jats:styled-content style="fixed-case">HPA</jats:styled-content> systems. The frequencies of <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1b were 0·993 and 0·007, with 0·943 and 0·057 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐2a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐2b, 0·527 and 0·473 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐3a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐3b, 0·997 and 0·003 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐4a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐4b, 0·991 and 0·009 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐5a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐5b, 0·980 and 0·020 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐6wa and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐6wb, 0·508 and 0·492 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐15a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐15b and 0·994 and 0·006 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐21wa and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐21wb.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>One multiplex <jats:styled-content style="fixed-case">PCR</jats:styled-content>‐<jats:styled-content style="fixed-case">SBT</jats:styled-content> method for <jats:styled-content style="fixed-case">HPA</jats:styled-content>s was established and the data of the study could help to prevent and treat for alloimmune thrombocytopenia.</jats:p></jats:sec> |
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| author | Hong, X., Chen, S., Ying, Y., Liu, Y., Xu, X., He, J., Zhu, F. |
| author_facet | Hong, X., Chen, S., Ying, Y., Liu, Y., Xu, X., He, J., Zhu, F., Hong, X., Chen, S., Ying, Y., Liu, Y., Xu, X., He, J., Zhu, F. |
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| description | <jats:sec><jats:title>Background and Objectives</jats:title><jats:p>Human platelet alloantigen (<jats:styled-content style="fixed-case">HPA</jats:styled-content>) genotyping is important for the diagnosis and prevention the alloimmune platelet disorders. In this study, a simultaneous genotyping method for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to ‐28bw systems was established using multiplex <jats:styled-content style="fixed-case">PCR</jats:styled-content>‐<jats:styled-content style="fixed-case">SBT</jats:styled-content> and the frequencies of genotypes and alleles of <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to ‐28bw systems in the Zhejiang Han population were analysed.</jats:p></jats:sec><jats:sec><jats:title>Materials and Methods</jats:title><jats:p>The specific primers were designed according to the nucleotide sequences of <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to 28bw systems which are located in <jats:italic><jats:styled-content style="fixed-case">ITGB</jats:styled-content>3, <jats:styled-content style="fixed-case">GP</jats:styled-content>1<jats:styled-content style="fixed-case">BA</jats:styled-content>,<jats:styled-content style="fixed-case"> ITGA</jats:styled-content>2B, <jats:styled-content style="fixed-case">ITGA</jats:styled-content>2, <jats:styled-content style="fixed-case">GP</jats:styled-content>1<jats:styled-content style="fixed-case">BB</jats:styled-content></jats:italic> and <jats:italic><jats:styled-content style="fixed-case">CD</jats:styled-content>109,</jats:italic> respectively. The multiplex <jats:styled-content style="fixed-case">PCR</jats:styled-content> amplification systems were used, and then, the amplicons were purified and sequenced. A total of 335 healthy volunteer blood donors were detected.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The genotypes of ten reference samples from Platelet Immunology Workshop of <jats:styled-content style="fixed-case">ISBT</jats:styled-content> were in concordance with the known genotypes. Among the 28 <jats:styled-content style="fixed-case">HPA</jats:styled-content> systems, <jats:styled-content style="fixed-case">HPA</jats:styled-content> a and b alleles were found in <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to 6w, <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐15 and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐21w systems in the Chinese Han population, while only <jats:styled-content style="fixed-case">HPA</jats:styled-content> aa genotype was detected in the other <jats:styled-content style="fixed-case">HPA</jats:styled-content> systems. The frequencies of <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1b were 0·993 and 0·007, with 0·943 and 0·057 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐2a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐2b, 0·527 and 0·473 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐3a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐3b, 0·997 and 0·003 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐4a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐4b, 0·991 and 0·009 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐5a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐5b, 0·980 and 0·020 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐6wa and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐6wb, 0·508 and 0·492 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐15a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐15b and 0·994 and 0·006 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐21wa and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐21wb.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>One multiplex <jats:styled-content style="fixed-case">PCR</jats:styled-content>‐<jats:styled-content style="fixed-case">SBT</jats:styled-content> method for <jats:styled-content style="fixed-case">HPA</jats:styled-content>s was established and the data of the study could help to prevent and treat for alloimmune thrombocytopenia.</jats:p></jats:sec> |
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| series | Vox Sanguinis |
| source_id | 49 |
| spelling | Hong, X. Chen, S. Ying, Y. Liu, Y. Xu, X. He, J. Zhu, F. 0042-9007 1423-0410 Wiley Hematology General Medicine http://dx.doi.org/10.1111/vox.12507 <jats:sec><jats:title>Background and Objectives</jats:title><jats:p>Human platelet alloantigen (<jats:styled-content style="fixed-case">HPA</jats:styled-content>) genotyping is important for the diagnosis and prevention the alloimmune platelet disorders. In this study, a simultaneous genotyping method for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to ‐28bw systems was established using multiplex <jats:styled-content style="fixed-case">PCR</jats:styled-content>‐<jats:styled-content style="fixed-case">SBT</jats:styled-content> and the frequencies of genotypes and alleles of <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to ‐28bw systems in the Zhejiang Han population were analysed.</jats:p></jats:sec><jats:sec><jats:title>Materials and Methods</jats:title><jats:p>The specific primers were designed according to the nucleotide sequences of <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to 28bw systems which are located in <jats:italic><jats:styled-content style="fixed-case">ITGB</jats:styled-content>3, <jats:styled-content style="fixed-case">GP</jats:styled-content>1<jats:styled-content style="fixed-case">BA</jats:styled-content>,<jats:styled-content style="fixed-case"> ITGA</jats:styled-content>2B, <jats:styled-content style="fixed-case">ITGA</jats:styled-content>2, <jats:styled-content style="fixed-case">GP</jats:styled-content>1<jats:styled-content style="fixed-case">BB</jats:styled-content></jats:italic> and <jats:italic><jats:styled-content style="fixed-case">CD</jats:styled-content>109,</jats:italic> respectively. The multiplex <jats:styled-content style="fixed-case">PCR</jats:styled-content> amplification systems were used, and then, the amplicons were purified and sequenced. A total of 335 healthy volunteer blood donors were detected.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The genotypes of ten reference samples from Platelet Immunology Workshop of <jats:styled-content style="fixed-case">ISBT</jats:styled-content> were in concordance with the known genotypes. Among the 28 <jats:styled-content style="fixed-case">HPA</jats:styled-content> systems, <jats:styled-content style="fixed-case">HPA</jats:styled-content> a and b alleles were found in <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1 to 6w, <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐15 and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐21w systems in the Chinese Han population, while only <jats:styled-content style="fixed-case">HPA</jats:styled-content> aa genotype was detected in the other <jats:styled-content style="fixed-case">HPA</jats:styled-content> systems. The frequencies of <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐1b were 0·993 and 0·007, with 0·943 and 0·057 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐2a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐2b, 0·527 and 0·473 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐3a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐3b, 0·997 and 0·003 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐4a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐4b, 0·991 and 0·009 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐5a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐5b, 0·980 and 0·020 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐6wa and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐6wb, 0·508 and 0·492 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐15a and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐15b and 0·994 and 0·006 for <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐21wa and <jats:styled-content style="fixed-case">HPA</jats:styled-content>‐21wb.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>One multiplex <jats:styled-content style="fixed-case">PCR</jats:styled-content>‐<jats:styled-content style="fixed-case">SBT</jats:styled-content> method for <jats:styled-content style="fixed-case">HPA</jats:styled-content>s was established and the data of the study could help to prevent and treat for alloimmune thrombocytopenia.</jats:p></jats:sec> Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing Vox Sanguinis |
| spellingShingle | Hong, X., Chen, S., Ying, Y., Liu, Y., Xu, X., He, J., Zhu, F., Vox Sanguinis, Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing, Hematology, General Medicine |
| title | Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| title_full | Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| title_fullStr | Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| title_full_unstemmed | Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| title_short | Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| title_sort | simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| title_unstemmed | Simultaneous genotyping of human platelet alloantigen‐1 to 28bw systems by multiplex polymerase chain reaction sequence‐based typing |
| topic | Hematology, General Medicine |
| url | http://dx.doi.org/10.1111/vox.12507 |