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Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level
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| Zeitschriftentitel: | The Plant Journal |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | The Plant Journal, 76, 2013, 5, S. 742-753 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Li, Genqiao Yu, Ming Fang, Tilin Cao, Shuanghe Carver, Brett F. Yan, Liuling Li, Genqiao Yu, Ming Fang, Tilin Cao, Shuanghe Carver, Brett F. Yan, Liuling |
|---|---|
| author |
Li, Genqiao Yu, Ming Fang, Tilin Cao, Shuanghe Carver, Brett F. Yan, Liuling |
| spellingShingle |
Li, Genqiao Yu, Ming Fang, Tilin Cao, Shuanghe Carver, Brett F. Yan, Liuling The Plant Journal Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level Cell Biology Plant Science Genetics |
| author_sort |
li, genqiao |
| spelling |
Li, Genqiao Yu, Ming Fang, Tilin Cao, Shuanghe Carver, Brett F. Yan, Liuling 0960-7412 1365-313X Wiley Cell Biology Plant Science Genetics http://dx.doi.org/10.1111/tpj.12326 <jats:title>Summary</jats:title><jats:p>Winter wheat requires a period of low temperatures to accelerate flowering (vernalization). This requirement could make winter wheat more vulnerable to elevated global temperature via insufficient vernalization. All known vernalization genes are cloned according to qualitative variation in vernalization requirement between spring and winter wheat, but the genes controlling quantitative variation for more or less vernalization requirement among winter wheat cultivars remain unknown. We report here that the gene for the vernalization requirement duration in winter wheat was cloned using a <jats:styled-content style="fixed-case">BC</jats:styled-content><jats:sub>1</jats:sub>F<jats:sub>2:3</jats:sub> population that segregated in a 3:1 ratio of early‐flowering plants and late‐flowering plants after vernalization for 3 weeks. The positional cloning of the gene for vernalization requirement duration demonstrated that this trait is controlled by <jats:italic>Ta</jats:italic><jats:styled-content style="fixed-case">VRN</jats:styled-content>‐<jats:styled-content style="fixed-case">A</jats:styled-content>1 at the protein level. The <jats:styled-content style="fixed-case">A</jats:styled-content>la<jats:sup>180</jats:sup> in vrn‐<jats:styled-content style="fixed-case">A</jats:styled-content>1a, encoded by the dominant allele for 3–week vernalization, was mutated to <jats:styled-content style="fixed-case">V</jats:styled-content>al<jats:sup>180</jats:sup> in vrn‐<jats:styled-content style="fixed-case">A</jats:styled-content>1b, encoded by the recessive allele for 6–week vernalization. Further studies with <jats:italic>in vitro</jats:italic> protein pull‐down assays and immunoprecipitation analyses indicated that the mutated <jats:styled-content style="fixed-case">V</jats:styled-content>al<jats:sup>180</jats:sup> in vrn‐<jats:styled-content style="fixed-case">A</jats:styled-content>1b protein decreased the ability to bind with <jats:italic><jats:styled-content style="fixed-case">T</jats:styled-content>a</jats:italic><jats:styled-content style="fixed-case">HOX</jats:styled-content>1 (the first homeobox protein in <jats:italic>Triticum aestivum</jats:italic>). The direct binding of <jats:italic>Ta</jats:italic><jats:styled-content style="fixed-case">VRN</jats:styled-content>‐A1 and <jats:italic>Ta</jats:italic><jats:styled-content style="fixed-case">HOX</jats:styled-content>1 proteins was confirmed in the nucleus of living plant cells by bimolecular fluorescence complementation (<jats:styled-content style="fixed-case">B</jats:styled-content>i<jats:styled-content style="fixed-case">FC</jats:styled-content>) analyses. The <jats:italic>Ta<jats:styled-content style="fixed-case">HOX</jats:styled-content>1</jats:italic> gene was found to be upregulated by low temperatures, and to have a significant genetic effect on heading date, suggesting that <jats:italic>Ta<jats:styled-content style="fixed-case">HOX</jats:styled-content>1</jats:italic> functions in the flowering pathway in winter wheat.</jats:p> Vernalization requirement duration in winter wheat is controlled by <i><scp>T</scp>a</i><scp>VRN</scp>‐<scp>A</scp>1 at the protein level The Plant Journal |
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10.1111/tpj.12326 |
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Biologie |
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| imprint |
Wiley, 2013 |
| imprint_str_mv |
Wiley, 2013 |
| issn |
1365-313X 0960-7412 |
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1365-313X 0960-7412 |
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li2013vernalizationrequirementdurationinwinterwheatiscontrolledbytavrna1attheproteinlevel |
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2013 |
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Wiley |
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ai |
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The Plant Journal |
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49 |
| title |
Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level |
| title_unstemmed |
Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level |
| title_full |
Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level |
| title_fullStr |
Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level |
| title_full_unstemmed |
Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level |
| title_short |
Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level |
| title_sort |
vernalization requirement duration in winter wheat is controlled by <i><scp>t</scp>a</i><scp>vrn</scp>‐<scp>a</scp>1 at the protein level |
| topic |
Cell Biology Plant Science Genetics |
| url |
http://dx.doi.org/10.1111/tpj.12326 |
| publishDate |
2013 |
| physical |
742-753 |
| description |
<jats:title>Summary</jats:title><jats:p>Winter wheat requires a period of low temperatures to accelerate flowering (vernalization). This requirement could make winter wheat more vulnerable to elevated global temperature via insufficient vernalization. All known vernalization genes are cloned according to qualitative variation in vernalization requirement between spring and winter wheat, but the genes controlling quantitative variation for more or less vernalization requirement among winter wheat cultivars remain unknown. We report here that the gene for the vernalization requirement duration in winter wheat was cloned using a <jats:styled-content style="fixed-case">BC</jats:styled-content><jats:sub>1</jats:sub>F<jats:sub>2:3</jats:sub> population that segregated in a 3:1 ratio of early‐flowering plants and late‐flowering plants after vernalization for 3 weeks. The positional cloning of the gene for vernalization requirement duration demonstrated that this trait is controlled by <jats:italic>Ta</jats:italic><jats:styled-content style="fixed-case">VRN</jats:styled-content>‐<jats:styled-content style="fixed-case">A</jats:styled-content>1 at the protein level. The <jats:styled-content style="fixed-case">A</jats:styled-content>la<jats:sup>180</jats:sup> in vrn‐<jats:styled-content style="fixed-case">A</jats:styled-content>1a, encoded by the dominant allele for 3–week vernalization, was mutated to <jats:styled-content style="fixed-case">V</jats:styled-content>al<jats:sup>180</jats:sup> in vrn‐<jats:styled-content style="fixed-case">A</jats:styled-content>1b, encoded by the recessive allele for 6–week vernalization. Further studies with <jats:italic>in vitro</jats:italic> protein pull‐down assays and immunoprecipitation analyses indicated that the mutated <jats:styled-content style="fixed-case">V</jats:styled-content>al<jats:sup>180</jats:sup> in vrn‐<jats:styled-content style="fixed-case">A</jats:styled-content>1b protein decreased the ability to bind with <jats:italic><jats:styled-content style="fixed-case">T</jats:styled-content>a</jats:italic><jats:styled-content style="fixed-case">HOX</jats:styled-content>1 (the first homeobox protein in <jats:italic>Triticum aestivum</jats:italic>). The direct binding of <jats:italic>Ta</jats:italic><jats:styled-content style="fixed-case">VRN</jats:styled-content>‐A1 and <jats:italic>Ta</jats:italic><jats:styled-content style="fixed-case">HOX</jats:styled-content>1 proteins was confirmed in the nucleus of living plant cells by bimolecular fluorescence complementation (<jats:styled-content style="fixed-case">B</jats:styled-content>i<jats:styled-content style="fixed-case">FC</jats:styled-content>) analyses. The <jats:italic>Ta<jats:styled-content style="fixed-case">HOX</jats:styled-content>1</jats:italic> gene was found to be upregulated by low temperatures, and to have a significant genetic effect on heading date, suggesting that <jats:italic>Ta<jats:styled-content style="fixed-case">HOX</jats:styled-content>1</jats:italic> functions in the flowering pathway in winter wheat.</jats:p> |
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| description | <jats:title>Summary</jats:title><jats:p>Winter wheat requires a period of low temperatures to accelerate flowering (vernalization). This requirement could make winter wheat more vulnerable to elevated global temperature via insufficient vernalization. All known vernalization genes are cloned according to qualitative variation in vernalization requirement between spring and winter wheat, but the genes controlling quantitative variation for more or less vernalization requirement among winter wheat cultivars remain unknown. We report here that the gene for the vernalization requirement duration in winter wheat was cloned using a <jats:styled-content style="fixed-case">BC</jats:styled-content><jats:sub>1</jats:sub>F<jats:sub>2:3</jats:sub> population that segregated in a 3:1 ratio of early‐flowering plants and late‐flowering plants after vernalization for 3 weeks. The positional cloning of the gene for vernalization requirement duration demonstrated that this trait is controlled by <jats:italic>Ta</jats:italic><jats:styled-content style="fixed-case">VRN</jats:styled-content>‐<jats:styled-content style="fixed-case">A</jats:styled-content>1 at the protein level. The <jats:styled-content style="fixed-case">A</jats:styled-content>la<jats:sup>180</jats:sup> in vrn‐<jats:styled-content style="fixed-case">A</jats:styled-content>1a, encoded by the dominant allele for 3–week vernalization, was mutated to <jats:styled-content style="fixed-case">V</jats:styled-content>al<jats:sup>180</jats:sup> in vrn‐<jats:styled-content style="fixed-case">A</jats:styled-content>1b, encoded by the recessive allele for 6–week vernalization. Further studies with <jats:italic>in vitro</jats:italic> protein pull‐down assays and immunoprecipitation analyses indicated that the mutated <jats:styled-content style="fixed-case">V</jats:styled-content>al<jats:sup>180</jats:sup> in vrn‐<jats:styled-content style="fixed-case">A</jats:styled-content>1b protein decreased the ability to bind with <jats:italic><jats:styled-content style="fixed-case">T</jats:styled-content>a</jats:italic><jats:styled-content style="fixed-case">HOX</jats:styled-content>1 (the first homeobox protein in <jats:italic>Triticum aestivum</jats:italic>). The direct binding of <jats:italic>Ta</jats:italic><jats:styled-content style="fixed-case">VRN</jats:styled-content>‐A1 and <jats:italic>Ta</jats:italic><jats:styled-content style="fixed-case">HOX</jats:styled-content>1 proteins was confirmed in the nucleus of living plant cells by bimolecular fluorescence complementation (<jats:styled-content style="fixed-case">B</jats:styled-content>i<jats:styled-content style="fixed-case">FC</jats:styled-content>) analyses. The <jats:italic>Ta<jats:styled-content style="fixed-case">HOX</jats:styled-content>1</jats:italic> gene was found to be upregulated by low temperatures, and to have a significant genetic effect on heading date, suggesting that <jats:italic>Ta<jats:styled-content style="fixed-case">HOX</jats:styled-content>1</jats:italic> functions in the flowering pathway in winter wheat.</jats:p> |
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| spelling | Li, Genqiao Yu, Ming Fang, Tilin Cao, Shuanghe Carver, Brett F. Yan, Liuling 0960-7412 1365-313X Wiley Cell Biology Plant Science Genetics http://dx.doi.org/10.1111/tpj.12326 <jats:title>Summary</jats:title><jats:p>Winter wheat requires a period of low temperatures to accelerate flowering (vernalization). This requirement could make winter wheat more vulnerable to elevated global temperature via insufficient vernalization. All known vernalization genes are cloned according to qualitative variation in vernalization requirement between spring and winter wheat, but the genes controlling quantitative variation for more or less vernalization requirement among winter wheat cultivars remain unknown. We report here that the gene for the vernalization requirement duration in winter wheat was cloned using a <jats:styled-content style="fixed-case">BC</jats:styled-content><jats:sub>1</jats:sub>F<jats:sub>2:3</jats:sub> population that segregated in a 3:1 ratio of early‐flowering plants and late‐flowering plants after vernalization for 3 weeks. The positional cloning of the gene for vernalization requirement duration demonstrated that this trait is controlled by <jats:italic>Ta</jats:italic><jats:styled-content style="fixed-case">VRN</jats:styled-content>‐<jats:styled-content style="fixed-case">A</jats:styled-content>1 at the protein level. The <jats:styled-content style="fixed-case">A</jats:styled-content>la<jats:sup>180</jats:sup> in vrn‐<jats:styled-content style="fixed-case">A</jats:styled-content>1a, encoded by the dominant allele for 3–week vernalization, was mutated to <jats:styled-content style="fixed-case">V</jats:styled-content>al<jats:sup>180</jats:sup> in vrn‐<jats:styled-content style="fixed-case">A</jats:styled-content>1b, encoded by the recessive allele for 6–week vernalization. Further studies with <jats:italic>in vitro</jats:italic> protein pull‐down assays and immunoprecipitation analyses indicated that the mutated <jats:styled-content style="fixed-case">V</jats:styled-content>al<jats:sup>180</jats:sup> in vrn‐<jats:styled-content style="fixed-case">A</jats:styled-content>1b protein decreased the ability to bind with <jats:italic><jats:styled-content style="fixed-case">T</jats:styled-content>a</jats:italic><jats:styled-content style="fixed-case">HOX</jats:styled-content>1 (the first homeobox protein in <jats:italic>Triticum aestivum</jats:italic>). The direct binding of <jats:italic>Ta</jats:italic><jats:styled-content style="fixed-case">VRN</jats:styled-content>‐A1 and <jats:italic>Ta</jats:italic><jats:styled-content style="fixed-case">HOX</jats:styled-content>1 proteins was confirmed in the nucleus of living plant cells by bimolecular fluorescence complementation (<jats:styled-content style="fixed-case">B</jats:styled-content>i<jats:styled-content style="fixed-case">FC</jats:styled-content>) analyses. The <jats:italic>Ta<jats:styled-content style="fixed-case">HOX</jats:styled-content>1</jats:italic> gene was found to be upregulated by low temperatures, and to have a significant genetic effect on heading date, suggesting that <jats:italic>Ta<jats:styled-content style="fixed-case">HOX</jats:styled-content>1</jats:italic> functions in the flowering pathway in winter wheat.</jats:p> Vernalization requirement duration in winter wheat is controlled by <i><scp>T</scp>a</i><scp>VRN</scp>‐<scp>A</scp>1 at the protein level The Plant Journal |
| spellingShingle | Li, Genqiao, Yu, Ming, Fang, Tilin, Cao, Shuanghe, Carver, Brett F., Yan, Liuling, The Plant Journal, Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level, Cell Biology, Plant Science, Genetics |
| title | Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level |
| title_full | Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level |
| title_fullStr | Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level |
| title_full_unstemmed | Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level |
| title_short | Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level |
| title_sort | vernalization requirement duration in winter wheat is controlled by <i><scp>t</scp>a</i><scp>vrn</scp>‐<scp>a</scp>1 at the protein level |
| title_unstemmed | Vernalization requirement duration in winter wheat is controlled by TaVRN‐A1 at the protein level |
| topic | Cell Biology, Plant Science, Genetics |
| url | http://dx.doi.org/10.1111/tpj.12326 |