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Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation
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| Zeitschriftentitel: | Plant Physiology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Plant Physiology, 139, 2005, 4, S. 1995-2005 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Oxford University Press (OUP)
|
| Schlagwörter: |
| author_facet |
Bölling, Christian Fiehn, Oliver Bölling, Christian Fiehn, Oliver |
|---|---|
| author |
Bölling, Christian Fiehn, Oliver |
| spellingShingle |
Bölling, Christian Fiehn, Oliver Plant Physiology Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation Plant Science Genetics Physiology |
| author_sort |
bölling, christian |
| spelling |
Bölling, Christian Fiehn, Oliver 1532-2548 0032-0889 Oxford University Press (OUP) Plant Science Genetics Physiology http://dx.doi.org/10.1104/pp.105.071589 <jats:title>Abstract</jats:title> <jats:p>A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result of the rapid sampling, extraction, and analysis by gas chromatography coupled to time-of-flight mass spectrometry, more than 800 analytes from a single sample could be measured, of which more than 100 could be identified. Analyte responses could be determined mostly with ses less than 10%. Wild-type cells of C. reinhardtii strain CC-125 subjected to nitrogen-, phosphorus-, sulfur-, or iron-depleted growth conditions develop highly distinctive metabolite profiles. Individual metabolites undergo marked changes in their steady-state levels. Compared to control conditions, sulfur-depleted cells accumulated 4-hydroxyproline more than 50-fold, whereas the amount of 2-ketovaline was reduced to 2% of control levels. The contribution of each compound to the differences observed in the metabolic phenotypes is summarized in a quantitatively rigorous way by principal component analysis, which clearly discriminates the cells from different growth regimes and indicates that phosphorus-depleted conditions induce a deficiency syndrome quite different from the response to nitrogen, sulfur, or iron starvation.</jats:p> Metabolite Profiling of <i>Chlamydomonas reinhardtii</i> under Nutrient Deprivation Plant Physiology |
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Plant Physiology |
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| title |
Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation |
| title_unstemmed |
Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation |
| title_full |
Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation |
| title_fullStr |
Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation |
| title_full_unstemmed |
Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation |
| title_short |
Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation |
| title_sort |
metabolite profiling of <i>chlamydomonas reinhardtii</i> under nutrient deprivation |
| topic |
Plant Science Genetics Physiology |
| url |
http://dx.doi.org/10.1104/pp.105.071589 |
| publishDate |
2005 |
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1995-2005 |
| description |
<jats:title>Abstract</jats:title>
<jats:p>A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result of the rapid sampling, extraction, and analysis by gas chromatography coupled to time-of-flight mass spectrometry, more than 800 analytes from a single sample could be measured, of which more than 100 could be identified. Analyte responses could be determined mostly with ses less than 10%. Wild-type cells of C. reinhardtii strain CC-125 subjected to nitrogen-, phosphorus-, sulfur-, or iron-depleted growth conditions develop highly distinctive metabolite profiles. Individual metabolites undergo marked changes in their steady-state levels. Compared to control conditions, sulfur-depleted cells accumulated 4-hydroxyproline more than 50-fold, whereas the amount of 2-ketovaline was reduced to 2% of control levels. The contribution of each compound to the differences observed in the metabolic phenotypes is summarized in a quantitatively rigorous way by principal component analysis, which clearly discriminates the cells from different growth regimes and indicates that phosphorus-depleted conditions induce a deficiency syndrome quite different from the response to nitrogen, sulfur, or iron starvation.</jats:p> |
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| author | Bölling, Christian, Fiehn, Oliver |
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| description | <jats:title>Abstract</jats:title> <jats:p>A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result of the rapid sampling, extraction, and analysis by gas chromatography coupled to time-of-flight mass spectrometry, more than 800 analytes from a single sample could be measured, of which more than 100 could be identified. Analyte responses could be determined mostly with ses less than 10%. Wild-type cells of C. reinhardtii strain CC-125 subjected to nitrogen-, phosphorus-, sulfur-, or iron-depleted growth conditions develop highly distinctive metabolite profiles. Individual metabolites undergo marked changes in their steady-state levels. Compared to control conditions, sulfur-depleted cells accumulated 4-hydroxyproline more than 50-fold, whereas the amount of 2-ketovaline was reduced to 2% of control levels. The contribution of each compound to the differences observed in the metabolic phenotypes is summarized in a quantitatively rigorous way by principal component analysis, which clearly discriminates the cells from different growth regimes and indicates that phosphorus-depleted conditions induce a deficiency syndrome quite different from the response to nitrogen, sulfur, or iron starvation.</jats:p> |
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| spelling | Bölling, Christian Fiehn, Oliver 1532-2548 0032-0889 Oxford University Press (OUP) Plant Science Genetics Physiology http://dx.doi.org/10.1104/pp.105.071589 <jats:title>Abstract</jats:title> <jats:p>A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result of the rapid sampling, extraction, and analysis by gas chromatography coupled to time-of-flight mass spectrometry, more than 800 analytes from a single sample could be measured, of which more than 100 could be identified. Analyte responses could be determined mostly with ses less than 10%. Wild-type cells of C. reinhardtii strain CC-125 subjected to nitrogen-, phosphorus-, sulfur-, or iron-depleted growth conditions develop highly distinctive metabolite profiles. Individual metabolites undergo marked changes in their steady-state levels. Compared to control conditions, sulfur-depleted cells accumulated 4-hydroxyproline more than 50-fold, whereas the amount of 2-ketovaline was reduced to 2% of control levels. The contribution of each compound to the differences observed in the metabolic phenotypes is summarized in a quantitatively rigorous way by principal component analysis, which clearly discriminates the cells from different growth regimes and indicates that phosphorus-depleted conditions induce a deficiency syndrome quite different from the response to nitrogen, sulfur, or iron starvation.</jats:p> Metabolite Profiling of <i>Chlamydomonas reinhardtii</i> under Nutrient Deprivation Plant Physiology |
| spellingShingle | Bölling, Christian, Fiehn, Oliver, Plant Physiology, Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation, Plant Science, Genetics, Physiology |
| title | Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation |
| title_full | Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation |
| title_fullStr | Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation |
| title_full_unstemmed | Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation |
| title_short | Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation |
| title_sort | metabolite profiling of <i>chlamydomonas reinhardtii</i> under nutrient deprivation |
| title_unstemmed | Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation |
| topic | Plant Science, Genetics, Physiology |
| url | http://dx.doi.org/10.1104/pp.105.071589 |