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Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Hi...
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| Zeitschriftentitel: | Plant Physiology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Plant Physiology, 129, 2002, 4, S. 1744-1754 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Oxford University Press (OUP)
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| Schlagwörter: |
| author_facet |
Thu-Hang, Pham Bassie, Ludovic Safwat, Gehan Trung-Nghia, Pham Christou, Paul Capell, Teresa Thu-Hang, Pham Bassie, Ludovic Safwat, Gehan Trung-Nghia, Pham Christou, Paul Capell, Teresa |
|---|---|
| author |
Thu-Hang, Pham Bassie, Ludovic Safwat, Gehan Trung-Nghia, Pham Christou, Paul Capell, Teresa |
| spellingShingle |
Thu-Hang, Pham Bassie, Ludovic Safwat, Gehan Trung-Nghia, Pham Christou, Paul Capell, Teresa Plant Physiology Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine Plant Science Genetics Physiology |
| author_sort |
thu-hang, pham |
| spelling |
Thu-Hang, Pham Bassie, Ludovic Safwat, Gehan Trung-Nghia, Pham Christou, Paul Capell, Teresa 1532-2548 0032-0889 Oxford University Press (OUP) Plant Science Genetics Physiology http://dx.doi.org/10.1104/pp.010966 <jats:title>Abstract</jats:title> <jats:p>We posed the question of whether steady-state levels of the higher polyamines spermidine and spermine in plants can be influenced by overexpression of a heterologous cDNA involved in the later steps of the pathway, in the absence of any further manipulation of the two synthases that are also involved in their biosynthesis. Transgenic rice (Oryza sativa) plants engineered with the heterologous Datura stramonium S-adenosylmethionine decarboxylase (samdc) cDNA exhibited accumulation of the transgene steady-state mRNA. Transgene expression did not affect expression of the orthologoussamdc gene. Significant increases in SAMDC activity translated to a direct increase in the level of spermidine, but not spermine, in leaves. Seeds recovered from a number of plants exhibited significant increases in spermidine and spermine levels. We demonstrate that overexpression of the D. stramonium samdc cDNA in transgenic rice is sufficient for accumulation of spermidine in leaves and spermidine and spermine in seeds. These findings suggest that increases in enzyme activity in one of the two components of the later parts of the pathway leading to the higher polyamines is sufficient to alter their levels mostly in seeds and, to some extent, in vegetative tissue such as leaves. Implications of our results on the design of rational approaches for the modulation of the polyamine pathway in plants are discussed in the general framework of metabolic pathway engineering.</jats:p> Expression of a Heterologous <i>S-Adenosylmethionine Decarboxylase</i> cDNA in Plants Demonstrates That Changes in<i>S</i>-Adenosyl-<scp>l</scp>-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine Plant Physiology |
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| title |
Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine |
| title_unstemmed |
Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine |
| title_full |
Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine |
| title_fullStr |
Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine |
| title_full_unstemmed |
Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine |
| title_short |
Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine |
| title_sort |
expression of a heterologous <i>s-adenosylmethionine decarboxylase</i> cdna in plants demonstrates that changes in<i>s</i>-adenosyl-<scp>l</scp>-methionine decarboxylase activity determine levels of the higher polyamines spermidine and spermine |
| topic |
Plant Science Genetics Physiology |
| url |
http://dx.doi.org/10.1104/pp.010966 |
| publishDate |
2002 |
| physical |
1744-1754 |
| description |
<jats:title>Abstract</jats:title>
<jats:p>We posed the question of whether steady-state levels of the higher polyamines spermidine and spermine in plants can be influenced by overexpression of a heterologous cDNA involved in the later steps of the pathway, in the absence of any further manipulation of the two synthases that are also involved in their biosynthesis. Transgenic rice (Oryza sativa) plants engineered with the heterologous Datura stramonium S-adenosylmethionine decarboxylase (samdc) cDNA exhibited accumulation of the transgene steady-state mRNA. Transgene expression did not affect expression of the orthologoussamdc gene. Significant increases in SAMDC activity translated to a direct increase in the level of spermidine, but not spermine, in leaves. Seeds recovered from a number of plants exhibited significant increases in spermidine and spermine levels. We demonstrate that overexpression of the D. stramonium samdc cDNA in transgenic rice is sufficient for accumulation of spermidine in leaves and spermidine and spermine in seeds. These findings suggest that increases in enzyme activity in one of the two components of the later parts of the pathway leading to the higher polyamines is sufficient to alter their levels mostly in seeds and, to some extent, in vegetative tissue such as leaves. Implications of our results on the design of rational approaches for the modulation of the polyamine pathway in plants are discussed in the general framework of metabolic pathway engineering.</jats:p> |
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| author | Thu-Hang, Pham, Bassie, Ludovic, Safwat, Gehan, Trung-Nghia, Pham, Christou, Paul, Capell, Teresa |
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| description | <jats:title>Abstract</jats:title> <jats:p>We posed the question of whether steady-state levels of the higher polyamines spermidine and spermine in plants can be influenced by overexpression of a heterologous cDNA involved in the later steps of the pathway, in the absence of any further manipulation of the two synthases that are also involved in their biosynthesis. Transgenic rice (Oryza sativa) plants engineered with the heterologous Datura stramonium S-adenosylmethionine decarboxylase (samdc) cDNA exhibited accumulation of the transgene steady-state mRNA. Transgene expression did not affect expression of the orthologoussamdc gene. Significant increases in SAMDC activity translated to a direct increase in the level of spermidine, but not spermine, in leaves. Seeds recovered from a number of plants exhibited significant increases in spermidine and spermine levels. We demonstrate that overexpression of the D. stramonium samdc cDNA in transgenic rice is sufficient for accumulation of spermidine in leaves and spermidine and spermine in seeds. These findings suggest that increases in enzyme activity in one of the two components of the later parts of the pathway leading to the higher polyamines is sufficient to alter their levels mostly in seeds and, to some extent, in vegetative tissue such as leaves. Implications of our results on the design of rational approaches for the modulation of the polyamine pathway in plants are discussed in the general framework of metabolic pathway engineering.</jats:p> |
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| spelling | Thu-Hang, Pham Bassie, Ludovic Safwat, Gehan Trung-Nghia, Pham Christou, Paul Capell, Teresa 1532-2548 0032-0889 Oxford University Press (OUP) Plant Science Genetics Physiology http://dx.doi.org/10.1104/pp.010966 <jats:title>Abstract</jats:title> <jats:p>We posed the question of whether steady-state levels of the higher polyamines spermidine and spermine in plants can be influenced by overexpression of a heterologous cDNA involved in the later steps of the pathway, in the absence of any further manipulation of the two synthases that are also involved in their biosynthesis. Transgenic rice (Oryza sativa) plants engineered with the heterologous Datura stramonium S-adenosylmethionine decarboxylase (samdc) cDNA exhibited accumulation of the transgene steady-state mRNA. Transgene expression did not affect expression of the orthologoussamdc gene. Significant increases in SAMDC activity translated to a direct increase in the level of spermidine, but not spermine, in leaves. Seeds recovered from a number of plants exhibited significant increases in spermidine and spermine levels. We demonstrate that overexpression of the D. stramonium samdc cDNA in transgenic rice is sufficient for accumulation of spermidine in leaves and spermidine and spermine in seeds. These findings suggest that increases in enzyme activity in one of the two components of the later parts of the pathway leading to the higher polyamines is sufficient to alter their levels mostly in seeds and, to some extent, in vegetative tissue such as leaves. Implications of our results on the design of rational approaches for the modulation of the polyamine pathway in plants are discussed in the general framework of metabolic pathway engineering.</jats:p> Expression of a Heterologous <i>S-Adenosylmethionine Decarboxylase</i> cDNA in Plants Demonstrates That Changes in<i>S</i>-Adenosyl-<scp>l</scp>-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine Plant Physiology |
| spellingShingle | Thu-Hang, Pham, Bassie, Ludovic, Safwat, Gehan, Trung-Nghia, Pham, Christou, Paul, Capell, Teresa, Plant Physiology, Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine, Plant Science, Genetics, Physiology |
| title | Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine |
| title_full | Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine |
| title_fullStr | Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine |
| title_full_unstemmed | Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine |
| title_short | Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine |
| title_sort | expression of a heterologous <i>s-adenosylmethionine decarboxylase</i> cdna in plants demonstrates that changes in<i>s</i>-adenosyl-<scp>l</scp>-methionine decarboxylase activity determine levels of the higher polyamines spermidine and spermine |
| title_unstemmed | Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes inS-Adenosyl-l-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine |
| topic | Plant Science, Genetics, Physiology |
| url | http://dx.doi.org/10.1104/pp.010966 |