Eintrag weiter verarbeiten
Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling
Gespeichert in:
| Zeitschriftentitel: | Genome Research |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Genome Research, 9, 1999, 12, S. 1305-1312 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
|
| Schlagwörter: |
| author_facet |
Kawamoto, Shoko Ohnishi, Tadashi Kita, Hiroko Chisaka, Osamu Okubo, Kousaku Kawamoto, Shoko Ohnishi, Tadashi Kita, Hiroko Chisaka, Osamu Okubo, Kousaku |
|---|---|
| author |
Kawamoto, Shoko Ohnishi, Tadashi Kita, Hiroko Chisaka, Osamu Okubo, Kousaku |
| spellingShingle |
Kawamoto, Shoko Ohnishi, Tadashi Kita, Hiroko Chisaka, Osamu Okubo, Kousaku Genome Research Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling Genetics (clinical) Genetics |
| author_sort |
kawamoto, shoko |
| spelling |
Kawamoto, Shoko Ohnishi, Tadashi Kita, Hiroko Chisaka, Osamu Okubo, Kousaku 1088-9051 1549-5469 Cold Spring Harbor Laboratory Genetics (clinical) Genetics http://dx.doi.org/10.1101/gr.9.12.1305 <jats:p>The availability of comprehensive sets of genes has prompted the researchers to carry out systematic collection of gene expression data. RT–PCR has the highest specificity and sensitivity for transcript detection among all available methods. Low throughput, especially when quantitative data are desired, has precluded RT–PCR from genome-wide application. Here we report a PCR-based expression profiling method, introduced amplified fragment length polymorphism (iAFLP), that has the same specificity and sensitivity as RT–PCR and a throughput level comparable to that of DNA–microarray hybridization. In this method, restricted ends of total cDNAs from six sources were ligated with adaptors having various length of short insertions to a common sequence (polymorphic adaptors). Amplification of a pool of these differentially adapted cDNAs with a gene-specific primer and an adaptor primer allows us to quantitate the abundance of any transcript in six mRNA sources. Using three different primer colors this technique allows quantitation of expression of 864 genes across six different sources per day with a single autosequencer, which is comparable to the throughput of microarray analysis in terms of number of genes × number of sources.</jats:p> Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling Genome Research |
| doi_str_mv |
10.1101/gr.9.12.1305 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwMS9nci45LjEyLjEzMDU |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwMS9nci45LjEyLjEzMDU |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 |
| imprint |
Cold Spring Harbor Laboratory, 1999 |
| imprint_str_mv |
Cold Spring Harbor Laboratory, 1999 |
| issn |
1088-9051 1549-5469 |
| issn_str_mv |
1088-9051 1549-5469 |
| language |
English |
| mega_collection |
Cold Spring Harbor Laboratory (CrossRef) |
| match_str |
kawamoto1999expressionprofilingbyiaflpapcrbasedmethodforgenomewidegeneexpressionprofiling |
| publishDateSort |
1999 |
| publisher |
Cold Spring Harbor Laboratory |
| recordtype |
ai |
| record_format |
ai |
| series |
Genome Research |
| source_id |
49 |
| title |
Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling |
| title_unstemmed |
Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling |
| title_full |
Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling |
| title_fullStr |
Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling |
| title_full_unstemmed |
Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling |
| title_short |
Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling |
| title_sort |
expression profiling by iaflp: a pcr-based method for genome-wide gene expression profiling |
| topic |
Genetics (clinical) Genetics |
| url |
http://dx.doi.org/10.1101/gr.9.12.1305 |
| publishDate |
1999 |
| physical |
1305-1312 |
| description |
<jats:p>The availability of comprehensive sets of genes has prompted the researchers to carry out systematic collection of gene expression data. RT–PCR has the highest specificity and sensitivity for transcript detection among all available methods. Low throughput, especially when quantitative data are desired, has precluded RT–PCR from genome-wide application. Here we report a PCR-based expression profiling method, introduced amplified fragment length polymorphism (iAFLP), that has the same specificity and sensitivity as RT–PCR and a throughput level comparable to that of DNA–microarray hybridization. In this method, restricted ends of total cDNAs from six sources were ligated with adaptors having various length of short insertions to a common sequence (polymorphic adaptors). Amplification of a pool of these differentially adapted cDNAs with a gene-specific primer and an adaptor primer allows us to quantitate the abundance of any transcript in six mRNA sources. Using three different primer colors this technique allows quantitation of expression of 864 genes across six different sources per day with a single autosequencer, which is comparable to the throughput of microarray analysis in terms of number of genes × number of sources.</jats:p> |
| container_issue |
12 |
| container_start_page |
1305 |
| container_title |
Genome Research |
| container_volume |
9 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792335420039626756 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T14:44:16.137Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Expression+Profiling+by+iAFLP%3A+A+PCR-Based+Method+for+Genome-Wide+Gene+Expression+Profiling&rft.date=1999-12-01&genre=article&issn=1549-5469&volume=9&issue=12&spage=1305&epage=1312&pages=1305-1312&jtitle=Genome+Research&atitle=Expression+Profiling+by+iAFLP%3A+A+PCR-Based+Method+for+Genome-Wide+Gene+Expression+Profiling&aulast=Okubo&aufirst=Kousaku&rft_id=info%3Adoi%2F10.1101%2Fgr.9.12.1305&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792335420039626756 |
| author | Kawamoto, Shoko, Ohnishi, Tadashi, Kita, Hiroko, Chisaka, Osamu, Okubo, Kousaku |
| author_facet | Kawamoto, Shoko, Ohnishi, Tadashi, Kita, Hiroko, Chisaka, Osamu, Okubo, Kousaku, Kawamoto, Shoko, Ohnishi, Tadashi, Kita, Hiroko, Chisaka, Osamu, Okubo, Kousaku |
| author_sort | kawamoto, shoko |
| container_issue | 12 |
| container_start_page | 1305 |
| container_title | Genome Research |
| container_volume | 9 |
| description | <jats:p>The availability of comprehensive sets of genes has prompted the researchers to carry out systematic collection of gene expression data. RT–PCR has the highest specificity and sensitivity for transcript detection among all available methods. Low throughput, especially when quantitative data are desired, has precluded RT–PCR from genome-wide application. Here we report a PCR-based expression profiling method, introduced amplified fragment length polymorphism (iAFLP), that has the same specificity and sensitivity as RT–PCR and a throughput level comparable to that of DNA–microarray hybridization. In this method, restricted ends of total cDNAs from six sources were ligated with adaptors having various length of short insertions to a common sequence (polymorphic adaptors). Amplification of a pool of these differentially adapted cDNAs with a gene-specific primer and an adaptor primer allows us to quantitate the abundance of any transcript in six mRNA sources. Using three different primer colors this technique allows quantitation of expression of 864 genes across six different sources per day with a single autosequencer, which is comparable to the throughput of microarray analysis in terms of number of genes × number of sources.</jats:p> |
| doi_str_mv | 10.1101/gr.9.12.1305 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwMS9nci45LjEyLjEzMDU |
| imprint | Cold Spring Harbor Laboratory, 1999 |
| imprint_str_mv | Cold Spring Harbor Laboratory, 1999 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 1088-9051, 1549-5469 |
| issn_str_mv | 1088-9051, 1549-5469 |
| language | English |
| last_indexed | 2024-03-01T14:44:16.137Z |
| match_str | kawamoto1999expressionprofilingbyiaflpapcrbasedmethodforgenomewidegeneexpressionprofiling |
| mega_collection | Cold Spring Harbor Laboratory (CrossRef) |
| physical | 1305-1312 |
| publishDate | 1999 |
| publishDateSort | 1999 |
| publisher | Cold Spring Harbor Laboratory |
| record_format | ai |
| recordtype | ai |
| series | Genome Research |
| source_id | 49 |
| spelling | Kawamoto, Shoko Ohnishi, Tadashi Kita, Hiroko Chisaka, Osamu Okubo, Kousaku 1088-9051 1549-5469 Cold Spring Harbor Laboratory Genetics (clinical) Genetics http://dx.doi.org/10.1101/gr.9.12.1305 <jats:p>The availability of comprehensive sets of genes has prompted the researchers to carry out systematic collection of gene expression data. RT–PCR has the highest specificity and sensitivity for transcript detection among all available methods. Low throughput, especially when quantitative data are desired, has precluded RT–PCR from genome-wide application. Here we report a PCR-based expression profiling method, introduced amplified fragment length polymorphism (iAFLP), that has the same specificity and sensitivity as RT–PCR and a throughput level comparable to that of DNA–microarray hybridization. In this method, restricted ends of total cDNAs from six sources were ligated with adaptors having various length of short insertions to a common sequence (polymorphic adaptors). Amplification of a pool of these differentially adapted cDNAs with a gene-specific primer and an adaptor primer allows us to quantitate the abundance of any transcript in six mRNA sources. Using three different primer colors this technique allows quantitation of expression of 864 genes across six different sources per day with a single autosequencer, which is comparable to the throughput of microarray analysis in terms of number of genes × number of sources.</jats:p> Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling Genome Research |
| spellingShingle | Kawamoto, Shoko, Ohnishi, Tadashi, Kita, Hiroko, Chisaka, Osamu, Okubo, Kousaku, Genome Research, Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling, Genetics (clinical), Genetics |
| title | Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling |
| title_full | Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling |
| title_fullStr | Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling |
| title_full_unstemmed | Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling |
| title_short | Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling |
| title_sort | expression profiling by iaflp: a pcr-based method for genome-wide gene expression profiling |
| title_unstemmed | Expression Profiling by iAFLP: A PCR-Based Method for Genome-Wide Gene Expression Profiling |
| topic | Genetics (clinical), Genetics |
| url | http://dx.doi.org/10.1101/gr.9.12.1305 |