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Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma.
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| Zeitschriftentitel: | Genes & Development |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Genes & Development, 4, 1990, 5, S. 789-805 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
|
| Schlagwörter: |
| author_facet |
Mattox, W Palmer, M J Baker, B S Mattox, W Palmer, M J Baker, B S |
|---|---|
| author |
Mattox, W Palmer, M J Baker, B S |
| spellingShingle |
Mattox, W Palmer, M J Baker, B S Genes & Development Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. Developmental Biology Genetics |
| author_sort |
mattox, w |
| spelling |
Mattox, W Palmer, M J Baker, B S 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.4.5.789 <jats:p>The transformer-2 (tra-2) gene of Drosophila melanogaster plays essential roles in both sexual differentiation in the female soma and spermatogenesis in the male germ line. In the female soma, tra-2 is known to act with other genes in the sex determination regulatory cascade to control the sex-specific alternative splicing of transcripts from the doublesex gene. Here, we determine whether or not any sex-specific tra-2 products are expressed that may account for either of these sex-specific activities. Sequence analysis of the tra-2 gene and 10 tra-2 cDNA clones coupled with nuclease protection analysis reveals a variety of alternatively spliced tra-2 mRNAs that each encode one of four distinct but overlapping polypeptides. Three of the encoded polypeptides contain both a ribonucleoprotein consensus sequence and arginine/serine-rich regions, suggesting a direct role for these products in RNA splicing. We show that although two transcripts are expressed male specifically in the germ line, the tra-2 transcripts expressed in the soma are not sex-specific. The translation of products from a tra-2-lacZ fusion gene in both sexes suggests that the female-specific functioning of tra-2 in somatic tissues is not attributable to a translational mechanism. We suggest that tra-2 activity in somatic tissues is regulated through a post-translational sex-specific interaction with the product of the tra gene rather than through the expression of a female-specific tra-2 polypeptide.</jats:p> Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. Genes & Development |
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| title |
Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| title_unstemmed |
Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| title_full |
Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| title_fullStr |
Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| title_full_unstemmed |
Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| title_short |
Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| title_sort |
alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| topic |
Developmental Biology Genetics |
| url |
http://dx.doi.org/10.1101/gad.4.5.789 |
| publishDate |
1990 |
| physical |
789-805 |
| description |
<jats:p>The transformer-2 (tra-2) gene of Drosophila melanogaster plays essential roles in both sexual differentiation in the female soma and spermatogenesis in the male germ line. In the female soma, tra-2 is known to act with other genes in the sex determination regulatory cascade to control the sex-specific alternative splicing of transcripts from the doublesex gene. Here, we determine whether or not any sex-specific tra-2 products are expressed that may account for either of these sex-specific activities. Sequence analysis of the tra-2 gene and 10 tra-2 cDNA clones coupled with nuclease protection analysis reveals a variety of alternatively spliced tra-2 mRNAs that each encode one of four distinct but overlapping polypeptides. Three of the encoded polypeptides contain both a ribonucleoprotein consensus sequence and arginine/serine-rich regions, suggesting a direct role for these products in RNA splicing. We show that although two transcripts are expressed male specifically in the germ line, the tra-2 transcripts expressed in the soma are not sex-specific. The translation of products from a tra-2-lacZ fusion gene in both sexes suggests that the female-specific functioning of tra-2 in somatic tissues is not attributable to a translational mechanism. We suggest that tra-2 activity in somatic tissues is regulated through a post-translational sex-specific interaction with the product of the tra gene rather than through the expression of a female-specific tra-2 polypeptide.</jats:p> |
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| description | <jats:p>The transformer-2 (tra-2) gene of Drosophila melanogaster plays essential roles in both sexual differentiation in the female soma and spermatogenesis in the male germ line. In the female soma, tra-2 is known to act with other genes in the sex determination regulatory cascade to control the sex-specific alternative splicing of transcripts from the doublesex gene. Here, we determine whether or not any sex-specific tra-2 products are expressed that may account for either of these sex-specific activities. Sequence analysis of the tra-2 gene and 10 tra-2 cDNA clones coupled with nuclease protection analysis reveals a variety of alternatively spliced tra-2 mRNAs that each encode one of four distinct but overlapping polypeptides. Three of the encoded polypeptides contain both a ribonucleoprotein consensus sequence and arginine/serine-rich regions, suggesting a direct role for these products in RNA splicing. We show that although two transcripts are expressed male specifically in the germ line, the tra-2 transcripts expressed in the soma are not sex-specific. The translation of products from a tra-2-lacZ fusion gene in both sexes suggests that the female-specific functioning of tra-2 in somatic tissues is not attributable to a translational mechanism. We suggest that tra-2 activity in somatic tissues is regulated through a post-translational sex-specific interaction with the product of the tra gene rather than through the expression of a female-specific tra-2 polypeptide.</jats:p> |
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| spelling | Mattox, W Palmer, M J Baker, B S 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.4.5.789 <jats:p>The transformer-2 (tra-2) gene of Drosophila melanogaster plays essential roles in both sexual differentiation in the female soma and spermatogenesis in the male germ line. In the female soma, tra-2 is known to act with other genes in the sex determination regulatory cascade to control the sex-specific alternative splicing of transcripts from the doublesex gene. Here, we determine whether or not any sex-specific tra-2 products are expressed that may account for either of these sex-specific activities. Sequence analysis of the tra-2 gene and 10 tra-2 cDNA clones coupled with nuclease protection analysis reveals a variety of alternatively spliced tra-2 mRNAs that each encode one of four distinct but overlapping polypeptides. Three of the encoded polypeptides contain both a ribonucleoprotein consensus sequence and arginine/serine-rich regions, suggesting a direct role for these products in RNA splicing. We show that although two transcripts are expressed male specifically in the germ line, the tra-2 transcripts expressed in the soma are not sex-specific. The translation of products from a tra-2-lacZ fusion gene in both sexes suggests that the female-specific functioning of tra-2 in somatic tissues is not attributable to a translational mechanism. We suggest that tra-2 activity in somatic tissues is regulated through a post-translational sex-specific interaction with the product of the tra gene rather than through the expression of a female-specific tra-2 polypeptide.</jats:p> Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. Genes & Development |
| spellingShingle | Mattox, W, Palmer, M J, Baker, B S, Genes & Development, Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma., Developmental Biology, Genetics |
| title | Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| title_full | Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| title_fullStr | Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| title_full_unstemmed | Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| title_short | Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| title_sort | alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| title_unstemmed | Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. |
| topic | Developmental Biology, Genetics |
| url | http://dx.doi.org/10.1101/gad.4.5.789 |