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Intergenic transcription through a Polycomb group response element counteracts silencing
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| Zeitschriftentitel: | Genes & Development |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Genes & Development, 19, 2005, 6, S. 697-708 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
|
| Schlagwörter: |
| author_facet |
Schmitt, Sabine Prestel, Matthias Paro, Renato Schmitt, Sabine Prestel, Matthias Paro, Renato |
|---|---|
| author |
Schmitt, Sabine Prestel, Matthias Paro, Renato |
| spellingShingle |
Schmitt, Sabine Prestel, Matthias Paro, Renato Genes & Development Intergenic transcription through a Polycomb group response element counteracts silencing Developmental Biology Genetics |
| author_sort |
schmitt, sabine |
| spelling |
Schmitt, Sabine Prestel, Matthias Paro, Renato 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.326205 <jats:p>Polycomb group response elements (PREs) mediate the mitotic inheritance of gene expression programs and thus maintain determined cell fates. By default, PREs silence associated genes via the targeting of Polycomb group (PcG) complexes. Upon an activating signal, however, PREs recruit counteracting trithorax group (trxG) proteins, which in turn maintain target genes in a transcriptionally active state. Using a transgenic reporter system, we show that the switch from the silenced to the activated state of a PRE requires noncoding transcription. Continuous transcription through the PRE induced by an <jats:italic>actin</jats:italic> promoter prevents the establishment of PcG-mediated silencing. The maintenance of epigenetic activation requires transcription through the PRE to proceed at least until embryogenesis is completed. At the homeotic bithorax complex of <jats:italic>Drosophila</jats:italic>, intergenic PRE transcripts can be detected not only during embryogenesis, but also at late larval stages, suggesting that transcription through endogenous PREs is required continuously as an anti-silencing mechanism to prevent the access of repressive PcG complexes to the chromatin. Furthermore, all other PREs outside the homeotic complex we tested were found to be transcribed in the same tissue as the mRNA of the corresponding target gene, suggesting that anti-silencing by transcription is a fundamental aspect of the cellular memory system.</jats:p> Intergenic transcription through a Polycomb group response element counteracts silencing Genes & Development |
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10.1101/gad.326205 |
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Cold Spring Harbor Laboratory, 2005 |
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Genes & Development |
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| title |
Intergenic transcription through a Polycomb group response element counteracts silencing |
| title_unstemmed |
Intergenic transcription through a Polycomb group response element counteracts silencing |
| title_full |
Intergenic transcription through a Polycomb group response element counteracts silencing |
| title_fullStr |
Intergenic transcription through a Polycomb group response element counteracts silencing |
| title_full_unstemmed |
Intergenic transcription through a Polycomb group response element counteracts silencing |
| title_short |
Intergenic transcription through a Polycomb group response element counteracts silencing |
| title_sort |
intergenic transcription through a polycomb group response element counteracts silencing |
| topic |
Developmental Biology Genetics |
| url |
http://dx.doi.org/10.1101/gad.326205 |
| publishDate |
2005 |
| physical |
697-708 |
| description |
<jats:p>Polycomb group response elements (PREs) mediate the mitotic inheritance of gene expression programs and thus maintain determined cell fates. By default, PREs silence associated genes via the targeting of Polycomb group (PcG) complexes. Upon an activating signal, however, PREs recruit counteracting trithorax group (trxG) proteins, which in turn maintain target genes in a transcriptionally active state. Using a transgenic reporter system, we show that the switch from the silenced to the activated state of a PRE requires noncoding transcription. Continuous transcription through the PRE induced by an <jats:italic>actin</jats:italic> promoter prevents the establishment of PcG-mediated silencing. The maintenance of epigenetic activation requires transcription through the PRE to proceed at least until embryogenesis is completed. At the homeotic bithorax complex of <jats:italic>Drosophila</jats:italic>, intergenic PRE transcripts can be detected not only during embryogenesis, but also at late larval stages, suggesting that transcription through endogenous PREs is required continuously as an anti-silencing mechanism to prevent the access of repressive PcG complexes to the chromatin. Furthermore, all other PREs outside the homeotic complex we tested were found to be transcribed in the same tissue as the mRNA of the corresponding target gene, suggesting that anti-silencing by transcription is a fundamental aspect of the cellular memory system.</jats:p> |
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| author | Schmitt, Sabine, Prestel, Matthias, Paro, Renato |
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| description | <jats:p>Polycomb group response elements (PREs) mediate the mitotic inheritance of gene expression programs and thus maintain determined cell fates. By default, PREs silence associated genes via the targeting of Polycomb group (PcG) complexes. Upon an activating signal, however, PREs recruit counteracting trithorax group (trxG) proteins, which in turn maintain target genes in a transcriptionally active state. Using a transgenic reporter system, we show that the switch from the silenced to the activated state of a PRE requires noncoding transcription. Continuous transcription through the PRE induced by an <jats:italic>actin</jats:italic> promoter prevents the establishment of PcG-mediated silencing. The maintenance of epigenetic activation requires transcription through the PRE to proceed at least until embryogenesis is completed. At the homeotic bithorax complex of <jats:italic>Drosophila</jats:italic>, intergenic PRE transcripts can be detected not only during embryogenesis, but also at late larval stages, suggesting that transcription through endogenous PREs is required continuously as an anti-silencing mechanism to prevent the access of repressive PcG complexes to the chromatin. Furthermore, all other PREs outside the homeotic complex we tested were found to be transcribed in the same tissue as the mRNA of the corresponding target gene, suggesting that anti-silencing by transcription is a fundamental aspect of the cellular memory system.</jats:p> |
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| institution | DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
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| spelling | Schmitt, Sabine Prestel, Matthias Paro, Renato 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.326205 <jats:p>Polycomb group response elements (PREs) mediate the mitotic inheritance of gene expression programs and thus maintain determined cell fates. By default, PREs silence associated genes via the targeting of Polycomb group (PcG) complexes. Upon an activating signal, however, PREs recruit counteracting trithorax group (trxG) proteins, which in turn maintain target genes in a transcriptionally active state. Using a transgenic reporter system, we show that the switch from the silenced to the activated state of a PRE requires noncoding transcription. Continuous transcription through the PRE induced by an <jats:italic>actin</jats:italic> promoter prevents the establishment of PcG-mediated silencing. The maintenance of epigenetic activation requires transcription through the PRE to proceed at least until embryogenesis is completed. At the homeotic bithorax complex of <jats:italic>Drosophila</jats:italic>, intergenic PRE transcripts can be detected not only during embryogenesis, but also at late larval stages, suggesting that transcription through endogenous PREs is required continuously as an anti-silencing mechanism to prevent the access of repressive PcG complexes to the chromatin. Furthermore, all other PREs outside the homeotic complex we tested were found to be transcribed in the same tissue as the mRNA of the corresponding target gene, suggesting that anti-silencing by transcription is a fundamental aspect of the cellular memory system.</jats:p> Intergenic transcription through a Polycomb group response element counteracts silencing Genes & Development |
| spellingShingle | Schmitt, Sabine, Prestel, Matthias, Paro, Renato, Genes & Development, Intergenic transcription through a Polycomb group response element counteracts silencing, Developmental Biology, Genetics |
| title | Intergenic transcription through a Polycomb group response element counteracts silencing |
| title_full | Intergenic transcription through a Polycomb group response element counteracts silencing |
| title_fullStr | Intergenic transcription through a Polycomb group response element counteracts silencing |
| title_full_unstemmed | Intergenic transcription through a Polycomb group response element counteracts silencing |
| title_short | Intergenic transcription through a Polycomb group response element counteracts silencing |
| title_sort | intergenic transcription through a polycomb group response element counteracts silencing |
| title_unstemmed | Intergenic transcription through a Polycomb group response element counteracts silencing |
| topic | Developmental Biology, Genetics |
| url | http://dx.doi.org/10.1101/gad.326205 |