Eintrag weiter verarbeiten
A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase
Gespeichert in:
| Zeitschriftentitel: | Genes & Development |
|---|---|
| Personen und Körperschaften: | , |
| In: | Genes & Development, 19, 2005, 1, S. 77-89 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cold Spring Harbor Laboratory
|
| Schlagwörter: |
| author_facet |
Mochizuki, Kazufumi Gorovsky, Martin A. Mochizuki, Kazufumi Gorovsky, Martin A. |
|---|---|
| author |
Mochizuki, Kazufumi Gorovsky, Martin A. |
| spellingShingle |
Mochizuki, Kazufumi Gorovsky, Martin A. Genes & Development A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase Developmental Biology Genetics |
| author_sort |
mochizuki, kazufumi |
| spelling |
Mochizuki, Kazufumi Gorovsky, Martin A. 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.1265105 <jats:p>Previous studies indicated that genome rearrangement involving DNA sequence elimination that occurs at late stages of conjugation in <jats:italic>Tetrahymena</jats:italic> is epigenetically controlled by siRNA-like scan (scn) RNAs produced from nongenic, heterogeneous, bidirectional, micronuclear transcripts synthesized at early stages of conjugation. Here, we show that Dcl1p, one of three <jats:italic>Tetrahymena</jats:italic> Dicer-like enzymes, is required for processing the micronuclear transcripts to scnRNAs. <jats:italic>DCL1</jats:italic> is also required for methylation of histone H3 at Lys 9, which, in wild-type cells, specifically occurs on the sequences (IESs) being eliminated. These results argue that Dcl1p processes nongenic micronuclear transcripts to scnRNAs and is required for IES elimination. This is the first evidence linking nongenic micronuclear transcripts, scnRNAs, and genome rearrangement. Dcl1p also is required for proper mitotic and meiotic segregation of micronuclear chromosomes and for normal chromosome alignment in meiotic prophase, suggesting that <jats:italic>DCL1</jats:italic> has multiple functions in regulating chromosome dynamics.</jats:p> A Dicer-like protein in <i>Tetrahymena</i> has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase Genes & Development |
| doi_str_mv |
10.1101/gad.1265105 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwMS9nYWQuMTI2NTEwNQ |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwMS9nYWQuMTI2NTEwNQ |
| institution |
DE-Zi4 DE-Gla1 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 |
| imprint |
Cold Spring Harbor Laboratory, 2005 |
| imprint_str_mv |
Cold Spring Harbor Laboratory, 2005 |
| issn |
0890-9369 1549-5477 |
| issn_str_mv |
0890-9369 1549-5477 |
| language |
English |
| mega_collection |
Cold Spring Harbor Laboratory (CrossRef) |
| match_str |
mochizuki2005adicerlikeproteinintetrahymenahasdistinctfunctionsingenomerearrangementchromosomesegregationandmeioticprophase |
| publishDateSort |
2005 |
| publisher |
Cold Spring Harbor Laboratory |
| recordtype |
ai |
| record_format |
ai |
| series |
Genes & Development |
| source_id |
49 |
| title |
A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| title_unstemmed |
A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| title_full |
A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| title_fullStr |
A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| title_full_unstemmed |
A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| title_short |
A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| title_sort |
a dicer-like protein in <i>tetrahymena</i> has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| topic |
Developmental Biology Genetics |
| url |
http://dx.doi.org/10.1101/gad.1265105 |
| publishDate |
2005 |
| physical |
77-89 |
| description |
<jats:p>Previous studies indicated that genome rearrangement involving DNA sequence elimination that occurs at late stages of conjugation in <jats:italic>Tetrahymena</jats:italic> is epigenetically controlled by siRNA-like scan (scn) RNAs produced from nongenic, heterogeneous, bidirectional, micronuclear transcripts synthesized at early stages of conjugation. Here, we show that Dcl1p, one of three <jats:italic>Tetrahymena</jats:italic> Dicer-like enzymes, is required for processing the micronuclear transcripts to scnRNAs. <jats:italic>DCL1</jats:italic> is also required for methylation of histone H3 at Lys 9, which, in wild-type cells, specifically occurs on the sequences (IESs) being eliminated. These results argue that Dcl1p processes nongenic micronuclear transcripts to scnRNAs and is required for IES elimination. This is the first evidence linking nongenic micronuclear transcripts, scnRNAs, and genome rearrangement. Dcl1p also is required for proper mitotic and meiotic segregation of micronuclear chromosomes and for normal chromosome alignment in meiotic prophase, suggesting that <jats:italic>DCL1</jats:italic> has multiple functions in regulating chromosome dynamics.</jats:p> |
| container_issue |
1 |
| container_start_page |
77 |
| container_title |
Genes & Development |
| container_volume |
19 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792346383153365002 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:38:31.404Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=A+Dicer-like+protein+in+Tetrahymena+has+distinct+functions+in+genome+rearrangement%2C+chromosome+segregation%2C+and+meiotic+prophase&rft.date=2005-01-01&genre=article&issn=1549-5477&volume=19&issue=1&spage=77&epage=89&pages=77-89&jtitle=Genes+%26+Development&atitle=A+Dicer-like+protein+in+%3Ci%3ETetrahymena%3C%2Fi%3E+has+distinct+functions+in+genome+rearrangement%2C+chromosome+segregation%2C+and+meiotic+prophase&aulast=Gorovsky&aufirst=Martin+A.&rft_id=info%3Adoi%2F10.1101%2Fgad.1265105&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792346383153365002 |
| author | Mochizuki, Kazufumi, Gorovsky, Martin A. |
| author_facet | Mochizuki, Kazufumi, Gorovsky, Martin A., Mochizuki, Kazufumi, Gorovsky, Martin A. |
| author_sort | mochizuki, kazufumi |
| container_issue | 1 |
| container_start_page | 77 |
| container_title | Genes & Development |
| container_volume | 19 |
| description | <jats:p>Previous studies indicated that genome rearrangement involving DNA sequence elimination that occurs at late stages of conjugation in <jats:italic>Tetrahymena</jats:italic> is epigenetically controlled by siRNA-like scan (scn) RNAs produced from nongenic, heterogeneous, bidirectional, micronuclear transcripts synthesized at early stages of conjugation. Here, we show that Dcl1p, one of three <jats:italic>Tetrahymena</jats:italic> Dicer-like enzymes, is required for processing the micronuclear transcripts to scnRNAs. <jats:italic>DCL1</jats:italic> is also required for methylation of histone H3 at Lys 9, which, in wild-type cells, specifically occurs on the sequences (IESs) being eliminated. These results argue that Dcl1p processes nongenic micronuclear transcripts to scnRNAs and is required for IES elimination. This is the first evidence linking nongenic micronuclear transcripts, scnRNAs, and genome rearrangement. Dcl1p also is required for proper mitotic and meiotic segregation of micronuclear chromosomes and for normal chromosome alignment in meiotic prophase, suggesting that <jats:italic>DCL1</jats:italic> has multiple functions in regulating chromosome dynamics.</jats:p> |
| doi_str_mv | 10.1101/gad.1265105 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTEwMS9nYWQuMTI2NTEwNQ |
| imprint | Cold Spring Harbor Laboratory, 2005 |
| imprint_str_mv | Cold Spring Harbor Laboratory, 2005 |
| institution | DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 0890-9369, 1549-5477 |
| issn_str_mv | 0890-9369, 1549-5477 |
| language | English |
| last_indexed | 2024-03-01T17:38:31.404Z |
| match_str | mochizuki2005adicerlikeproteinintetrahymenahasdistinctfunctionsingenomerearrangementchromosomesegregationandmeioticprophase |
| mega_collection | Cold Spring Harbor Laboratory (CrossRef) |
| physical | 77-89 |
| publishDate | 2005 |
| publishDateSort | 2005 |
| publisher | Cold Spring Harbor Laboratory |
| record_format | ai |
| recordtype | ai |
| series | Genes & Development |
| source_id | 49 |
| spelling | Mochizuki, Kazufumi Gorovsky, Martin A. 0890-9369 1549-5477 Cold Spring Harbor Laboratory Developmental Biology Genetics http://dx.doi.org/10.1101/gad.1265105 <jats:p>Previous studies indicated that genome rearrangement involving DNA sequence elimination that occurs at late stages of conjugation in <jats:italic>Tetrahymena</jats:italic> is epigenetically controlled by siRNA-like scan (scn) RNAs produced from nongenic, heterogeneous, bidirectional, micronuclear transcripts synthesized at early stages of conjugation. Here, we show that Dcl1p, one of three <jats:italic>Tetrahymena</jats:italic> Dicer-like enzymes, is required for processing the micronuclear transcripts to scnRNAs. <jats:italic>DCL1</jats:italic> is also required for methylation of histone H3 at Lys 9, which, in wild-type cells, specifically occurs on the sequences (IESs) being eliminated. These results argue that Dcl1p processes nongenic micronuclear transcripts to scnRNAs and is required for IES elimination. This is the first evidence linking nongenic micronuclear transcripts, scnRNAs, and genome rearrangement. Dcl1p also is required for proper mitotic and meiotic segregation of micronuclear chromosomes and for normal chromosome alignment in meiotic prophase, suggesting that <jats:italic>DCL1</jats:italic> has multiple functions in regulating chromosome dynamics.</jats:p> A Dicer-like protein in <i>Tetrahymena</i> has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase Genes & Development |
| spellingShingle | Mochizuki, Kazufumi, Gorovsky, Martin A., Genes & Development, A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase, Developmental Biology, Genetics |
| title | A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| title_full | A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| title_fullStr | A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| title_full_unstemmed | A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| title_short | A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| title_sort | a dicer-like protein in <i>tetrahymena</i> has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| title_unstemmed | A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase |
| topic | Developmental Biology, Genetics |
| url | http://dx.doi.org/10.1101/gad.1265105 |