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Histochemical demonstration of prolactin binding sites.
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| Zeitschriftentitel: | Journal of Histochemistry & Cytochemistry |
|---|---|
| Personen und Körperschaften: | , |
| In: | Journal of Histochemistry & Cytochemistry, 37, 1989, 6, S. 773-779 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
SAGE Publications
|
| Schlagwörter: |
| author_facet |
Michel, E Parsons, J A Michel, E Parsons, J A |
|---|---|
| author |
Michel, E Parsons, J A |
| spellingShingle |
Michel, E Parsons, J A Journal of Histochemistry & Cytochemistry Histochemical demonstration of prolactin binding sites. Histology Anatomy |
| author_sort |
michel, e |
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Michel, E Parsons, J A 0022-1554 1551-5044 SAGE Publications Histology Anatomy http://dx.doi.org/10.1177/37.6.2723398 <jats:p> We have developed a new probe for histochemical demonstration of prolactin binding sites. Ovine prolactin (oPRL) was conjugated with the N-hydroxy-succinimide ester derivative of the fluorochrome 7-amino-4-methylcoumarin-3-acetic acid. Under mild reaction conditions the ester derivative reacted with available NH2 groups of the prolactin molecule to form stable bonds. The coupling reaction yielded products that co-migrated with oPRL but had slightly decreased isoelectric points. The receptor binding and bioactivity of the flurochrome-hormone derivatives were decreased essentially proportional to the extent of conjugation. The derivatives were further tested for their ability to label PRL binding sites, using frozen sections of mammary gland and brain tissue of lactating rats. The results presented in this report describe the validation of this probe with regard to labeling of PRL binding sites at the light microscopic level in known target organs (mammary gland and choroid plexus). In addition, this fluorescent probe was used to demonstrate the presence of PRL binding sites at a novel site, the ependymal lining of the third ventricle. </jats:p> Histochemical demonstration of prolactin binding sites. Journal of Histochemistry & Cytochemistry |
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Journal of Histochemistry & Cytochemistry |
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| title |
Histochemical demonstration of prolactin binding sites. |
| title_unstemmed |
Histochemical demonstration of prolactin binding sites. |
| title_full |
Histochemical demonstration of prolactin binding sites. |
| title_fullStr |
Histochemical demonstration of prolactin binding sites. |
| title_full_unstemmed |
Histochemical demonstration of prolactin binding sites. |
| title_short |
Histochemical demonstration of prolactin binding sites. |
| title_sort |
histochemical demonstration of prolactin binding sites. |
| topic |
Histology Anatomy |
| url |
http://dx.doi.org/10.1177/37.6.2723398 |
| publishDate |
1989 |
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773-779 |
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<jats:p> We have developed a new probe for histochemical demonstration of prolactin binding sites. Ovine prolactin (oPRL) was conjugated with the N-hydroxy-succinimide ester derivative of the fluorochrome 7-amino-4-methylcoumarin-3-acetic acid. Under mild reaction conditions the ester derivative reacted with available NH2 groups of the prolactin molecule to form stable bonds. The coupling reaction yielded products that co-migrated with oPRL but had slightly decreased isoelectric points. The receptor binding and bioactivity of the flurochrome-hormone derivatives were decreased essentially proportional to the extent of conjugation. The derivatives were further tested for their ability to label PRL binding sites, using frozen sections of mammary gland and brain tissue of lactating rats. The results presented in this report describe the validation of this probe with regard to labeling of PRL binding sites at the light microscopic level in known target organs (mammary gland and choroid plexus). In addition, this fluorescent probe was used to demonstrate the presence of PRL binding sites at a novel site, the ependymal lining of the third ventricle. </jats:p> |
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| description | <jats:p> We have developed a new probe for histochemical demonstration of prolactin binding sites. Ovine prolactin (oPRL) was conjugated with the N-hydroxy-succinimide ester derivative of the fluorochrome 7-amino-4-methylcoumarin-3-acetic acid. Under mild reaction conditions the ester derivative reacted with available NH2 groups of the prolactin molecule to form stable bonds. The coupling reaction yielded products that co-migrated with oPRL but had slightly decreased isoelectric points. The receptor binding and bioactivity of the flurochrome-hormone derivatives were decreased essentially proportional to the extent of conjugation. The derivatives were further tested for their ability to label PRL binding sites, using frozen sections of mammary gland and brain tissue of lactating rats. The results presented in this report describe the validation of this probe with regard to labeling of PRL binding sites at the light microscopic level in known target organs (mammary gland and choroid plexus). In addition, this fluorescent probe was used to demonstrate the presence of PRL binding sites at a novel site, the ependymal lining of the third ventricle. </jats:p> |
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| imprint | SAGE Publications, 1989 |
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| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| physical | 773-779 |
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| series | Journal of Histochemistry & Cytochemistry |
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| spelling | Michel, E Parsons, J A 0022-1554 1551-5044 SAGE Publications Histology Anatomy http://dx.doi.org/10.1177/37.6.2723398 <jats:p> We have developed a new probe for histochemical demonstration of prolactin binding sites. Ovine prolactin (oPRL) was conjugated with the N-hydroxy-succinimide ester derivative of the fluorochrome 7-amino-4-methylcoumarin-3-acetic acid. Under mild reaction conditions the ester derivative reacted with available NH2 groups of the prolactin molecule to form stable bonds. The coupling reaction yielded products that co-migrated with oPRL but had slightly decreased isoelectric points. The receptor binding and bioactivity of the flurochrome-hormone derivatives were decreased essentially proportional to the extent of conjugation. The derivatives were further tested for their ability to label PRL binding sites, using frozen sections of mammary gland and brain tissue of lactating rats. The results presented in this report describe the validation of this probe with regard to labeling of PRL binding sites at the light microscopic level in known target organs (mammary gland and choroid plexus). In addition, this fluorescent probe was used to demonstrate the presence of PRL binding sites at a novel site, the ependymal lining of the third ventricle. </jats:p> Histochemical demonstration of prolactin binding sites. Journal of Histochemistry & Cytochemistry |
| spellingShingle | Michel, E, Parsons, J A, Journal of Histochemistry & Cytochemistry, Histochemical demonstration of prolactin binding sites., Histology, Anatomy |
| title | Histochemical demonstration of prolactin binding sites. |
| title_full | Histochemical demonstration of prolactin binding sites. |
| title_fullStr | Histochemical demonstration of prolactin binding sites. |
| title_full_unstemmed | Histochemical demonstration of prolactin binding sites. |
| title_short | Histochemical demonstration of prolactin binding sites. |
| title_sort | histochemical demonstration of prolactin binding sites. |
| title_unstemmed | Histochemical demonstration of prolactin binding sites. |
| topic | Histology, Anatomy |
| url | http://dx.doi.org/10.1177/37.6.2723398 |