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Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver.
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| Zeitschriftentitel: | Journal of Histochemistry & Cytochemistry |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Journal of Histochemistry & Cytochemistry, 36, 1988, 2, S. 179-184 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
SAGE Publications
|
| Schlagwörter: |
| author_facet |
Richmond, R E Pereira, M A Carter, J H Carter, H W Long, R E Richmond, R E Pereira, M A Carter, J H Carter, H W Long, R E |
|---|---|
| author |
Richmond, R E Pereira, M A Carter, J H Carter, H W Long, R E |
| spellingShingle |
Richmond, R E Pereira, M A Carter, J H Carter, H W Long, R E Journal of Histochemistry & Cytochemistry Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. Histology Anatomy |
| author_sort |
richmond, r e |
| spelling |
Richmond, R E Pereira, M A Carter, J H Carter, H W Long, R E 0022-1554 1551-5044 SAGE Publications Histology Anatomy http://dx.doi.org/10.1177/36.2.3121722 <jats:p> This study examined the possibility of using an immunohistochemical technique to detect the expression of myc and src oncogene proteins (ops) in livers of male Sprague-Dawley rats after treatment with the carcinogen diethylnitrosamine (with or without phenobarbital promotion) or untreated. We found that the majority of nodules and tumors from these livers stained for myc and src ops, indicating that myc and src expression did occur in these structures. These results were expected, since myc and src expression has been previously observed by others using different techniques. However, in our study, myc and src op staining was also noted in normal liver areas from rats in any of the four treatment groups (DENA, DENA + PB, PB alone, or untreated). The staining pattern of normal liver was different for each oncogene probe but was consistent within the four groups. In most cases, oncogene expression of normal liver occurred at sites of abnormal (but non-neoplastic) hepatocytes. The method reported here used both a qualitative technique of op expression analysis and a quantitative method using a Zeiss computer-driven image analysis system. </jats:p> Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. Journal of Histochemistry & Cytochemistry |
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1988 |
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Journal of Histochemistry & Cytochemistry |
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49 |
| title |
Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| title_unstemmed |
Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| title_full |
Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| title_fullStr |
Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| title_full_unstemmed |
Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| title_short |
Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| title_sort |
quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| topic |
Histology Anatomy |
| url |
http://dx.doi.org/10.1177/36.2.3121722 |
| publishDate |
1988 |
| physical |
179-184 |
| description |
<jats:p> This study examined the possibility of using an immunohistochemical technique to detect the expression of myc and src oncogene proteins (ops) in livers of male Sprague-Dawley rats after treatment with the carcinogen diethylnitrosamine (with or without phenobarbital promotion) or untreated. We found that the majority of nodules and tumors from these livers stained for myc and src ops, indicating that myc and src expression did occur in these structures. These results were expected, since myc and src expression has been previously observed by others using different techniques. However, in our study, myc and src op staining was also noted in normal liver areas from rats in any of the four treatment groups (DENA, DENA + PB, PB alone, or untreated). The staining pattern of normal liver was different for each oncogene probe but was consistent within the four groups. In most cases, oncogene expression of normal liver occurred at sites of abnormal (but non-neoplastic) hepatocytes. The method reported here used both a qualitative technique of op expression analysis and a quantitative method using a Zeiss computer-driven image analysis system. </jats:p> |
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| author | Richmond, R E, Pereira, M A, Carter, J H, Carter, H W, Long, R E |
| author_facet | Richmond, R E, Pereira, M A, Carter, J H, Carter, H W, Long, R E, Richmond, R E, Pereira, M A, Carter, J H, Carter, H W, Long, R E |
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| description | <jats:p> This study examined the possibility of using an immunohistochemical technique to detect the expression of myc and src oncogene proteins (ops) in livers of male Sprague-Dawley rats after treatment with the carcinogen diethylnitrosamine (with or without phenobarbital promotion) or untreated. We found that the majority of nodules and tumors from these livers stained for myc and src ops, indicating that myc and src expression did occur in these structures. These results were expected, since myc and src expression has been previously observed by others using different techniques. However, in our study, myc and src op staining was also noted in normal liver areas from rats in any of the four treatment groups (DENA, DENA + PB, PB alone, or untreated). The staining pattern of normal liver was different for each oncogene probe but was consistent within the four groups. In most cases, oncogene expression of normal liver occurred at sites of abnormal (but non-neoplastic) hepatocytes. The method reported here used both a qualitative technique of op expression analysis and a quantitative method using a Zeiss computer-driven image analysis system. </jats:p> |
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| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE3Ny8zNi4yLjMxMjE3MjI |
| imprint | SAGE Publications, 1988 |
| imprint_str_mv | SAGE Publications, 1988 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14 |
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| physical | 179-184 |
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| series | Journal of Histochemistry & Cytochemistry |
| source_id | 49 |
| spelling | Richmond, R E Pereira, M A Carter, J H Carter, H W Long, R E 0022-1554 1551-5044 SAGE Publications Histology Anatomy http://dx.doi.org/10.1177/36.2.3121722 <jats:p> This study examined the possibility of using an immunohistochemical technique to detect the expression of myc and src oncogene proteins (ops) in livers of male Sprague-Dawley rats after treatment with the carcinogen diethylnitrosamine (with or without phenobarbital promotion) or untreated. We found that the majority of nodules and tumors from these livers stained for myc and src ops, indicating that myc and src expression did occur in these structures. These results were expected, since myc and src expression has been previously observed by others using different techniques. However, in our study, myc and src op staining was also noted in normal liver areas from rats in any of the four treatment groups (DENA, DENA + PB, PB alone, or untreated). The staining pattern of normal liver was different for each oncogene probe but was consistent within the four groups. In most cases, oncogene expression of normal liver occurred at sites of abnormal (but non-neoplastic) hepatocytes. The method reported here used both a qualitative technique of op expression analysis and a quantitative method using a Zeiss computer-driven image analysis system. </jats:p> Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. Journal of Histochemistry & Cytochemistry |
| spellingShingle | Richmond, R E, Pereira, M A, Carter, J H, Carter, H W, Long, R E, Journal of Histochemistry & Cytochemistry, Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver., Histology, Anatomy |
| title | Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| title_full | Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| title_fullStr | Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| title_full_unstemmed | Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| title_short | Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| title_sort | quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| title_unstemmed | Quantitative and qualitative immunohistochemical detection of myc and src oncogene proteins in normal, nodule, and neoplastic rat liver. |
| topic | Histology, Anatomy |
| url | http://dx.doi.org/10.1177/36.2.3121722 |