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N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma
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| Zeitschriftentitel: | European Journal of Inflammation |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | European Journal of Inflammation, 3, 2005, 1, S. 17-22 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
SAGE Publications
|
| Schlagwörter: |
| author_facet |
Laragione, T. Tonelli, R. D'Incalci, M. Colombo, T. Ghezzi, P. Laragione, T. Tonelli, R. D'Incalci, M. Colombo, T. Ghezzi, P. |
|---|---|
| author |
Laragione, T. Tonelli, R. D'Incalci, M. Colombo, T. Ghezzi, P. |
| spellingShingle |
Laragione, T. Tonelli, R. D'Incalci, M. Colombo, T. Ghezzi, P. European Journal of Inflammation N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma General Medicine Immunology Immunology and Allergy |
| author_sort |
laragione, t. |
| spelling |
Laragione, T. Tonelli, R. D'Incalci, M. Colombo, T. Ghezzi, P. 2058-7392 2058-7392 SAGE Publications General Medicine Immunology Immunology and Allergy http://dx.doi.org/10.1177/1721727x0500300104 <jats:p> The redox state of surface protein thiols influences a variety of cell functions, and we recently reported that adhesion molecules can be redox regulated. We investigated the effect of reducing surface thiols using N-acetylcysteine (NAC) on the biological properties of murine melanoma B16F1 cells. Treating the cells with NAC (5mM for 2h, then removed by washing) augmented their capacity to adhere to fibronectin, as well as to adhere to and invade an endothelial cell monolayer. This was associated with an augmented expression of reduced surface protein thiols. However, when control or NAC-pretreated melanoma cells were injected i.v. in mice to induce experimental lung metastases, we could observe an inhibition of metastatic potential by NAC. This discrepancy suggest that other redox sensitive steps, in addition to adhesion, are important in regulating the metastatic phenotype in vivo. </jats:p> N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma European Journal of Inflammation |
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| title |
N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma |
| title_unstemmed |
N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma |
| title_full |
N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma |
| title_fullStr |
N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma |
| title_full_unstemmed |
N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma |
| title_short |
N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma |
| title_sort |
n-acetylcysteine augments surface thiols and differentially modulates cell adhesion and invasion in vitro and metastatic potential in vivo of b16f1 melanoma |
| topic |
General Medicine Immunology Immunology and Allergy |
| url |
http://dx.doi.org/10.1177/1721727x0500300104 |
| publishDate |
2005 |
| physical |
17-22 |
| description |
<jats:p> The redox state of surface protein thiols influences a variety of cell functions, and we recently reported that adhesion molecules can be redox regulated. We investigated the effect of reducing surface thiols using N-acetylcysteine (NAC) on the biological properties of murine melanoma B16F1 cells. Treating the cells with NAC (5mM for 2h, then removed by washing) augmented their capacity to adhere to fibronectin, as well as to adhere to and invade an endothelial cell monolayer. This was associated with an augmented expression of reduced surface protein thiols. However, when control or NAC-pretreated melanoma cells were injected i.v. in mice to induce experimental lung metastases, we could observe an inhibition of metastatic potential by NAC. This discrepancy suggest that other redox sensitive steps, in addition to adhesion, are important in regulating the metastatic phenotype in vivo. </jats:p> |
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| author | Laragione, T., Tonelli, R., D'Incalci, M., Colombo, T., Ghezzi, P. |
| author_facet | Laragione, T., Tonelli, R., D'Incalci, M., Colombo, T., Ghezzi, P., Laragione, T., Tonelli, R., D'Incalci, M., Colombo, T., Ghezzi, P. |
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| description | <jats:p> The redox state of surface protein thiols influences a variety of cell functions, and we recently reported that adhesion molecules can be redox regulated. We investigated the effect of reducing surface thiols using N-acetylcysteine (NAC) on the biological properties of murine melanoma B16F1 cells. Treating the cells with NAC (5mM for 2h, then removed by washing) augmented their capacity to adhere to fibronectin, as well as to adhere to and invade an endothelial cell monolayer. This was associated with an augmented expression of reduced surface protein thiols. However, when control or NAC-pretreated melanoma cells were injected i.v. in mice to induce experimental lung metastases, we could observe an inhibition of metastatic potential by NAC. This discrepancy suggest that other redox sensitive steps, in addition to adhesion, are important in regulating the metastatic phenotype in vivo. </jats:p> |
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| imprint_str_mv | SAGE Publications, 2005 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229 |
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| spelling | Laragione, T. Tonelli, R. D'Incalci, M. Colombo, T. Ghezzi, P. 2058-7392 2058-7392 SAGE Publications General Medicine Immunology Immunology and Allergy http://dx.doi.org/10.1177/1721727x0500300104 <jats:p> The redox state of surface protein thiols influences a variety of cell functions, and we recently reported that adhesion molecules can be redox regulated. We investigated the effect of reducing surface thiols using N-acetylcysteine (NAC) on the biological properties of murine melanoma B16F1 cells. Treating the cells with NAC (5mM for 2h, then removed by washing) augmented their capacity to adhere to fibronectin, as well as to adhere to and invade an endothelial cell monolayer. This was associated with an augmented expression of reduced surface protein thiols. However, when control or NAC-pretreated melanoma cells were injected i.v. in mice to induce experimental lung metastases, we could observe an inhibition of metastatic potential by NAC. This discrepancy suggest that other redox sensitive steps, in addition to adhesion, are important in regulating the metastatic phenotype in vivo. </jats:p> N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma European Journal of Inflammation |
| spellingShingle | Laragione, T., Tonelli, R., D'Incalci, M., Colombo, T., Ghezzi, P., European Journal of Inflammation, N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma, General Medicine, Immunology, Immunology and Allergy |
| title | N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma |
| title_full | N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma |
| title_fullStr | N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma |
| title_full_unstemmed | N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma |
| title_short | N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma |
| title_sort | n-acetylcysteine augments surface thiols and differentially modulates cell adhesion and invasion in vitro and metastatic potential in vivo of b16f1 melanoma |
| title_unstemmed | N-Acetylcysteine Augments Surface Thiols and Differentially Modulates Cell Adhesion and Invasion in vitro and Metastatic Potential in vivo of B16F1 Melanoma |
| topic | General Medicine, Immunology, Immunology and Allergy |
| url | http://dx.doi.org/10.1177/1721727x0500300104 |