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Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine
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| Zeitschriftentitel: | Circulation Research |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Circulation Research, 125, 2019, Suppl_1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Ovid Technologies (Wolters Kluwer Health)
|
| Schlagwörter: |
| author_facet |
Velayutham, Nivedhitha Alfieri, Christina M Agnew, Emma J Riggs, Kyle W Baker, Richard S Zafar, Farhan Yutzey, Katherine E Velayutham, Nivedhitha Alfieri, Christina M Agnew, Emma J Riggs, Kyle W Baker, Richard S Zafar, Farhan Yutzey, Katherine E |
|---|---|
| author |
Velayutham, Nivedhitha Alfieri, Christina M Agnew, Emma J Riggs, Kyle W Baker, Richard S Zafar, Farhan Yutzey, Katherine E |
| spellingShingle |
Velayutham, Nivedhitha Alfieri, Christina M Agnew, Emma J Riggs, Kyle W Baker, Richard S Zafar, Farhan Yutzey, Katherine E Circulation Research Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine Cardiology and Cardiovascular Medicine Physiology |
| author_sort |
velayutham, nivedhitha |
| spelling |
Velayutham, Nivedhitha Alfieri, Christina M Agnew, Emma J Riggs, Kyle W Baker, Richard S Zafar, Farhan Yutzey, Katherine E 0009-7330 1524-4571 Ovid Technologies (Wolters Kluwer Health) Cardiology and Cardiovascular Medicine Physiology http://dx.doi.org/10.1161/res.125.suppl_1.423 <jats:p> <jats:bold>Objectives:</jats:bold> Cardiomyocyte (CM) cell cycle arrest and decline of mononucleated diploid CMs have been implicated in loss of regenerative potential in postnatal mouse hearts. Sarcomeric and extracellular matrix (ECM) maturation also occur concurrently in mice, influencing CM proliferative arrest. Recent studies show a 3-day neonatal period of cardiac regeneration in pigs similar to mice, but the dynamics of postnatal pig CM growth are unknown. Our objective is to explore cardiac cell cycling, growth and maturation in postnatal pigs to understand the events guiding loss of cardiac regenerative capacity in large mammals. </jats:p> <jats:p> <jats:bold>Methods & Results:</jats:bold> Left-ventricular tissue from farm pigs (White Yorkshire-Landrace) at Postnatal day (P)0, P7, P15, P30, 2 months (2mo) and 6mo were utilized. CM dissociations revealed predominant CM mononucleation at birth in swine, with persistence of ~50% (1186/2537 cells, n=5) mononucleated CMs at P15, and ~10% (227/1785 cells, n=4) at 2mo. By 6mo, pig CMs are entirely multinucleated, exhibiting 4-16 nuclei per cell. Assessing hypertrophic growth in dissociated pig CMs revealed longitudinal CM growth relative to increased nucleation at all ages. However, onset of diametric hypertrophy only occurs beyond 2mo. When nuclear pHH3 and mRNA expression of cell cycle genes was assessed, pig hearts show robust cell-cycling up to 2mo. Also, fetal TNNI1 and MYH6 are active up to 2mo in pig hearts. Ongoing studies on collagen remodeling indicate ECM remodeling in swine occurs beyond P7. Future studies are designed to identify nuclear ploidy in postnatal pig CMs and measure cytokinesis. </jats:p> <jats:p> <jats:bold>Conclusions:</jats:bold> Cardiac maturational events are staggered over a 2-6 month postnatal window in pigs, with older pig hearts exhibiting extensive CM multinucleation and differences in longitudinal versus diametric CM growth. These fundamental variations in CM growth characteristics are important to consider when designing preclinical trials for cardiac regenerative strategies in pigs. Also, despite a similar period of regenerative capacity as mice, pig hearts do not undergo loss of CM cell cycling and mononucleation until 2mo after birth. Utilizing pigs may thus offer unique opportunities to study aspects of heart regeneration unavailable in other animal models. </jats:p> Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine Circulation Research |
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10.1161/res.125.suppl_1.423 |
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Ovid Technologies (Wolters Kluwer Health), 2019 |
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0009-7330 1524-4571 |
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| title |
Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine |
| title_unstemmed |
Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine |
| title_full |
Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine |
| title_fullStr |
Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine |
| title_full_unstemmed |
Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine |
| title_short |
Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine |
| title_sort |
abstract 423: cardiomyocyte maturation and multinucleation in postnatal swine |
| topic |
Cardiology and Cardiovascular Medicine Physiology |
| url |
http://dx.doi.org/10.1161/res.125.suppl_1.423 |
| publishDate |
2019 |
| physical |
|
| description |
<jats:p>
<jats:bold>Objectives:</jats:bold>
Cardiomyocyte (CM) cell cycle arrest and decline of mononucleated diploid CMs have been implicated in loss of regenerative potential in postnatal mouse hearts. Sarcomeric and extracellular matrix (ECM) maturation also occur concurrently in mice, influencing CM proliferative arrest. Recent studies show a 3-day neonatal period of cardiac regeneration in pigs similar to mice, but the dynamics of postnatal pig CM growth are unknown. Our objective is to explore cardiac cell cycling, growth and maturation in postnatal pigs to understand the events guiding loss of cardiac regenerative capacity in large mammals.
</jats:p>
<jats:p>
<jats:bold>Methods & Results:</jats:bold>
Left-ventricular tissue from farm pigs (White Yorkshire-Landrace) at Postnatal day (P)0, P7, P15, P30, 2 months (2mo) and 6mo were utilized. CM dissociations revealed predominant CM mononucleation at birth in swine, with persistence of ~50% (1186/2537 cells, n=5) mononucleated CMs at P15, and ~10% (227/1785 cells, n=4) at 2mo. By 6mo, pig CMs are entirely multinucleated, exhibiting 4-16 nuclei per cell. Assessing hypertrophic growth in dissociated pig CMs revealed longitudinal CM growth relative to increased nucleation at all ages. However, onset of diametric hypertrophy only occurs beyond 2mo. When nuclear pHH3 and mRNA expression of cell cycle genes was assessed, pig hearts show robust cell-cycling up to 2mo. Also, fetal TNNI1 and MYH6 are active up to 2mo in pig hearts. Ongoing studies on collagen remodeling indicate ECM remodeling in swine occurs beyond P7. Future studies are designed to identify nuclear ploidy in postnatal pig CMs and measure cytokinesis.
</jats:p>
<jats:p>
<jats:bold>Conclusions:</jats:bold>
Cardiac maturational events are staggered over a 2-6 month postnatal window in pigs, with older pig hearts exhibiting extensive CM multinucleation and differences in longitudinal versus diametric CM growth. These fundamental variations in CM growth characteristics are important to consider when designing preclinical trials for cardiac regenerative strategies in pigs. Also, despite a similar period of regenerative capacity as mice, pig hearts do not undergo loss of CM cell cycling and mononucleation until 2mo after birth. Utilizing pigs may thus offer unique opportunities to study aspects of heart regeneration unavailable in other animal models.
</jats:p> |
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| author | Velayutham, Nivedhitha, Alfieri, Christina M, Agnew, Emma J, Riggs, Kyle W, Baker, Richard S, Zafar, Farhan, Yutzey, Katherine E |
| author_facet | Velayutham, Nivedhitha, Alfieri, Christina M, Agnew, Emma J, Riggs, Kyle W, Baker, Richard S, Zafar, Farhan, Yutzey, Katherine E, Velayutham, Nivedhitha, Alfieri, Christina M, Agnew, Emma J, Riggs, Kyle W, Baker, Richard S, Zafar, Farhan, Yutzey, Katherine E |
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| description | <jats:p> <jats:bold>Objectives:</jats:bold> Cardiomyocyte (CM) cell cycle arrest and decline of mononucleated diploid CMs have been implicated in loss of regenerative potential in postnatal mouse hearts. Sarcomeric and extracellular matrix (ECM) maturation also occur concurrently in mice, influencing CM proliferative arrest. Recent studies show a 3-day neonatal period of cardiac regeneration in pigs similar to mice, but the dynamics of postnatal pig CM growth are unknown. Our objective is to explore cardiac cell cycling, growth and maturation in postnatal pigs to understand the events guiding loss of cardiac regenerative capacity in large mammals. </jats:p> <jats:p> <jats:bold>Methods & Results:</jats:bold> Left-ventricular tissue from farm pigs (White Yorkshire-Landrace) at Postnatal day (P)0, P7, P15, P30, 2 months (2mo) and 6mo were utilized. CM dissociations revealed predominant CM mononucleation at birth in swine, with persistence of ~50% (1186/2537 cells, n=5) mononucleated CMs at P15, and ~10% (227/1785 cells, n=4) at 2mo. By 6mo, pig CMs are entirely multinucleated, exhibiting 4-16 nuclei per cell. Assessing hypertrophic growth in dissociated pig CMs revealed longitudinal CM growth relative to increased nucleation at all ages. However, onset of diametric hypertrophy only occurs beyond 2mo. When nuclear pHH3 and mRNA expression of cell cycle genes was assessed, pig hearts show robust cell-cycling up to 2mo. Also, fetal TNNI1 and MYH6 are active up to 2mo in pig hearts. Ongoing studies on collagen remodeling indicate ECM remodeling in swine occurs beyond P7. Future studies are designed to identify nuclear ploidy in postnatal pig CMs and measure cytokinesis. </jats:p> <jats:p> <jats:bold>Conclusions:</jats:bold> Cardiac maturational events are staggered over a 2-6 month postnatal window in pigs, with older pig hearts exhibiting extensive CM multinucleation and differences in longitudinal versus diametric CM growth. These fundamental variations in CM growth characteristics are important to consider when designing preclinical trials for cardiac regenerative strategies in pigs. Also, despite a similar period of regenerative capacity as mice, pig hearts do not undergo loss of CM cell cycling and mononucleation until 2mo after birth. Utilizing pigs may thus offer unique opportunities to study aspects of heart regeneration unavailable in other animal models. </jats:p> |
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| spelling | Velayutham, Nivedhitha Alfieri, Christina M Agnew, Emma J Riggs, Kyle W Baker, Richard S Zafar, Farhan Yutzey, Katherine E 0009-7330 1524-4571 Ovid Technologies (Wolters Kluwer Health) Cardiology and Cardiovascular Medicine Physiology http://dx.doi.org/10.1161/res.125.suppl_1.423 <jats:p> <jats:bold>Objectives:</jats:bold> Cardiomyocyte (CM) cell cycle arrest and decline of mononucleated diploid CMs have been implicated in loss of regenerative potential in postnatal mouse hearts. Sarcomeric and extracellular matrix (ECM) maturation also occur concurrently in mice, influencing CM proliferative arrest. Recent studies show a 3-day neonatal period of cardiac regeneration in pigs similar to mice, but the dynamics of postnatal pig CM growth are unknown. Our objective is to explore cardiac cell cycling, growth and maturation in postnatal pigs to understand the events guiding loss of cardiac regenerative capacity in large mammals. </jats:p> <jats:p> <jats:bold>Methods & Results:</jats:bold> Left-ventricular tissue from farm pigs (White Yorkshire-Landrace) at Postnatal day (P)0, P7, P15, P30, 2 months (2mo) and 6mo were utilized. CM dissociations revealed predominant CM mononucleation at birth in swine, with persistence of ~50% (1186/2537 cells, n=5) mononucleated CMs at P15, and ~10% (227/1785 cells, n=4) at 2mo. By 6mo, pig CMs are entirely multinucleated, exhibiting 4-16 nuclei per cell. Assessing hypertrophic growth in dissociated pig CMs revealed longitudinal CM growth relative to increased nucleation at all ages. However, onset of diametric hypertrophy only occurs beyond 2mo. When nuclear pHH3 and mRNA expression of cell cycle genes was assessed, pig hearts show robust cell-cycling up to 2mo. Also, fetal TNNI1 and MYH6 are active up to 2mo in pig hearts. Ongoing studies on collagen remodeling indicate ECM remodeling in swine occurs beyond P7. Future studies are designed to identify nuclear ploidy in postnatal pig CMs and measure cytokinesis. </jats:p> <jats:p> <jats:bold>Conclusions:</jats:bold> Cardiac maturational events are staggered over a 2-6 month postnatal window in pigs, with older pig hearts exhibiting extensive CM multinucleation and differences in longitudinal versus diametric CM growth. These fundamental variations in CM growth characteristics are important to consider when designing preclinical trials for cardiac regenerative strategies in pigs. Also, despite a similar period of regenerative capacity as mice, pig hearts do not undergo loss of CM cell cycling and mononucleation until 2mo after birth. Utilizing pigs may thus offer unique opportunities to study aspects of heart regeneration unavailable in other animal models. </jats:p> Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine Circulation Research |
| spellingShingle | Velayutham, Nivedhitha, Alfieri, Christina M, Agnew, Emma J, Riggs, Kyle W, Baker, Richard S, Zafar, Farhan, Yutzey, Katherine E, Circulation Research, Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine, Cardiology and Cardiovascular Medicine, Physiology |
| title | Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine |
| title_full | Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine |
| title_fullStr | Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine |
| title_full_unstemmed | Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine |
| title_short | Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine |
| title_sort | abstract 423: cardiomyocyte maturation and multinucleation in postnatal swine |
| title_unstemmed | Abstract 423: Cardiomyocyte Maturation and Multinucleation in Postnatal Swine |
| topic | Cardiology and Cardiovascular Medicine, Physiology |
| url | http://dx.doi.org/10.1161/res.125.suppl_1.423 |