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Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes
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| Zeitschriftentitel: | Circulation Research |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Circulation Research, 125, 2019, Suppl_1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Ovid Technologies (Wolters Kluwer Health)
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| Schlagwörter: |
| author_facet |
Jeon, Young Keul Jang, Ji Hyun Youm, Jae Boum Zhang, Yin Hua Kim, Sung Joon Jeon, Young Keul Jang, Ji Hyun Youm, Jae Boum Zhang, Yin Hua Kim, Sung Joon |
|---|---|
| author |
Jeon, Young Keul Jang, Ji Hyun Youm, Jae Boum Zhang, Yin Hua Kim, Sung Joon |
| spellingShingle |
Jeon, Young Keul Jang, Ji Hyun Youm, Jae Boum Zhang, Yin Hua Kim, Sung Joon Circulation Research Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes Cardiology and Cardiovascular Medicine Physiology |
| author_sort |
jeon, young keul |
| spelling |
Jeon, Young Keul Jang, Ji Hyun Youm, Jae Boum Zhang, Yin Hua Kim, Sung Joon 0009-7330 1524-4571 Ovid Technologies (Wolters Kluwer Health) Cardiology and Cardiovascular Medicine Physiology http://dx.doi.org/10.1161/res.125.suppl_1.191 <jats:p> Left ventricle (LV) and right ventricle (RV) have distinctive structural and functional characteristics as well as heterogeneous physiological properties. The RV is exposed to a relatively low pulmonary vasculature, which results in less mechanical afterload. Consistent with these physiological differences, the RV has a thinner free wall than the LV, and the movement of its contraction is geometrically different. Despite these definite differences, the studies of basic excitation-contraction coupling and calcium homeostasis of RV has been less studied than in LV. To establish the interventricular difference, we evaluated the basic electrophysiological and calcium-contractile properties of myocyte with or without β-adrenergic stimulation. Analyses of contraction and Ca <jats:sup>2+</jats:sup> signaling and action potential duration (APD) in isolated RV myocytes showed more prominent APD prolongation with less significant changes in sarcomere shortening and calcium transient, implying less efficient E-C coupling RV myocytes. To investigate the prolonged APD of RV, we measured transient outward potassium (Ito) current and L-type calcium channel (LTCC) current of two ventricles. Although the peak current of two ion channel was not different significantly in two ventricle, the half inactivation voltage of RV Ito was smaller than LV. Comparing with LV, RV myocytes showed round peak, slower early relaxation, and faster late relaxation, suggesting the difference of calcium sensitivity between two ventricles. To investigate the difference, we examined the expression level of calcium-binding proteins that regulate myofilament activities. Using immunoblotting from enriched protein of myofilament fraction, we found that the calcium binding proteins such as troponin I were lower in RV. Taken together, our results suggest that calcium binding proteins of RV was differ from that of LV, which induce the modified calcium sensitivity. We confirmed that reduced calcium binding proteins induced the decrease of calcium sensitivity and modified E-C coupling using Bers’ mathematical rat cardiomyocyte model. The calcium sensitivity of RV is a clue to explain the different physiological properties of the RV. </jats:p> Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes Circulation Research |
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Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes |
| title_unstemmed |
Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes |
| title_full |
Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes |
| title_fullStr |
Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes |
| title_full_unstemmed |
Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes |
| title_short |
Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes |
| title_sort |
abstract 191: difference in calcium sensitivity between right and left ventricles with lower expression of calcium binding proteins in right ventricular myocytes |
| topic |
Cardiology and Cardiovascular Medicine Physiology |
| url |
http://dx.doi.org/10.1161/res.125.suppl_1.191 |
| publishDate |
2019 |
| physical |
|
| description |
<jats:p>
Left ventricle (LV) and right ventricle (RV) have distinctive structural and functional characteristics as well as heterogeneous physiological properties. The RV is exposed to a relatively low pulmonary vasculature, which results in less mechanical afterload. Consistent with these physiological differences, the RV has a thinner free wall than the LV, and the movement of its contraction is geometrically different. Despite these definite differences, the studies of basic excitation-contraction coupling and calcium homeostasis of RV has been less studied than in LV. To establish the interventricular difference, we evaluated the basic electrophysiological and calcium-contractile properties of myocyte with or without β-adrenergic stimulation. Analyses of contraction and Ca
<jats:sup>2+</jats:sup>
signaling and action potential duration (APD) in isolated RV myocytes showed more prominent APD prolongation with less significant changes in sarcomere shortening and calcium transient, implying less efficient E-C coupling RV myocytes. To investigate the prolonged APD of RV, we measured transient outward potassium (Ito) current and L-type calcium channel (LTCC) current of two ventricles. Although the peak current of two ion channel was not different significantly in two ventricle, the half inactivation voltage of RV Ito was smaller than LV. Comparing with LV, RV myocytes showed round peak, slower early relaxation, and faster late relaxation, suggesting the difference of calcium sensitivity between two ventricles. To investigate the difference, we examined the expression level of calcium-binding proteins that regulate myofilament activities. Using immunoblotting from enriched protein of myofilament fraction, we found that the calcium binding proteins such as troponin I were lower in RV. Taken together, our results suggest that calcium binding proteins of RV was differ from that of LV, which induce the modified calcium sensitivity. We confirmed that reduced calcium binding proteins induced the decrease of calcium sensitivity and modified E-C coupling using Bers’ mathematical rat cardiomyocyte model. The calcium sensitivity of RV is a clue to explain the different physiological properties of the RV.
</jats:p> |
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| description | <jats:p> Left ventricle (LV) and right ventricle (RV) have distinctive structural and functional characteristics as well as heterogeneous physiological properties. The RV is exposed to a relatively low pulmonary vasculature, which results in less mechanical afterload. Consistent with these physiological differences, the RV has a thinner free wall than the LV, and the movement of its contraction is geometrically different. Despite these definite differences, the studies of basic excitation-contraction coupling and calcium homeostasis of RV has been less studied than in LV. To establish the interventricular difference, we evaluated the basic electrophysiological and calcium-contractile properties of myocyte with or without β-adrenergic stimulation. Analyses of contraction and Ca <jats:sup>2+</jats:sup> signaling and action potential duration (APD) in isolated RV myocytes showed more prominent APD prolongation with less significant changes in sarcomere shortening and calcium transient, implying less efficient E-C coupling RV myocytes. To investigate the prolonged APD of RV, we measured transient outward potassium (Ito) current and L-type calcium channel (LTCC) current of two ventricles. Although the peak current of two ion channel was not different significantly in two ventricle, the half inactivation voltage of RV Ito was smaller than LV. Comparing with LV, RV myocytes showed round peak, slower early relaxation, and faster late relaxation, suggesting the difference of calcium sensitivity between two ventricles. To investigate the difference, we examined the expression level of calcium-binding proteins that regulate myofilament activities. Using immunoblotting from enriched protein of myofilament fraction, we found that the calcium binding proteins such as troponin I were lower in RV. Taken together, our results suggest that calcium binding proteins of RV was differ from that of LV, which induce the modified calcium sensitivity. We confirmed that reduced calcium binding proteins induced the decrease of calcium sensitivity and modified E-C coupling using Bers’ mathematical rat cardiomyocyte model. The calcium sensitivity of RV is a clue to explain the different physiological properties of the RV. </jats:p> |
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| spelling | Jeon, Young Keul Jang, Ji Hyun Youm, Jae Boum Zhang, Yin Hua Kim, Sung Joon 0009-7330 1524-4571 Ovid Technologies (Wolters Kluwer Health) Cardiology and Cardiovascular Medicine Physiology http://dx.doi.org/10.1161/res.125.suppl_1.191 <jats:p> Left ventricle (LV) and right ventricle (RV) have distinctive structural and functional characteristics as well as heterogeneous physiological properties. The RV is exposed to a relatively low pulmonary vasculature, which results in less mechanical afterload. Consistent with these physiological differences, the RV has a thinner free wall than the LV, and the movement of its contraction is geometrically different. Despite these definite differences, the studies of basic excitation-contraction coupling and calcium homeostasis of RV has been less studied than in LV. To establish the interventricular difference, we evaluated the basic electrophysiological and calcium-contractile properties of myocyte with or without β-adrenergic stimulation. Analyses of contraction and Ca <jats:sup>2+</jats:sup> signaling and action potential duration (APD) in isolated RV myocytes showed more prominent APD prolongation with less significant changes in sarcomere shortening and calcium transient, implying less efficient E-C coupling RV myocytes. To investigate the prolonged APD of RV, we measured transient outward potassium (Ito) current and L-type calcium channel (LTCC) current of two ventricles. Although the peak current of two ion channel was not different significantly in two ventricle, the half inactivation voltage of RV Ito was smaller than LV. Comparing with LV, RV myocytes showed round peak, slower early relaxation, and faster late relaxation, suggesting the difference of calcium sensitivity between two ventricles. To investigate the difference, we examined the expression level of calcium-binding proteins that regulate myofilament activities. Using immunoblotting from enriched protein of myofilament fraction, we found that the calcium binding proteins such as troponin I were lower in RV. Taken together, our results suggest that calcium binding proteins of RV was differ from that of LV, which induce the modified calcium sensitivity. We confirmed that reduced calcium binding proteins induced the decrease of calcium sensitivity and modified E-C coupling using Bers’ mathematical rat cardiomyocyte model. The calcium sensitivity of RV is a clue to explain the different physiological properties of the RV. </jats:p> Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes Circulation Research |
| spellingShingle | Jeon, Young Keul, Jang, Ji Hyun, Youm, Jae Boum, Zhang, Yin Hua, Kim, Sung Joon, Circulation Research, Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes, Cardiology and Cardiovascular Medicine, Physiology |
| title | Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes |
| title_full | Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes |
| title_fullStr | Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes |
| title_full_unstemmed | Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes |
| title_short | Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes |
| title_sort | abstract 191: difference in calcium sensitivity between right and left ventricles with lower expression of calcium binding proteins in right ventricular myocytes |
| title_unstemmed | Abstract 191: Difference in Calcium Sensitivity Between Right and Left Ventricles With Lower Expression of Calcium Binding Proteins in Right Ventricular Myocytes |
| topic | Cardiology and Cardiovascular Medicine, Physiology |
| url | http://dx.doi.org/10.1161/res.125.suppl_1.191 |