Eintrag weiter verarbeiten
Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats
Gespeichert in:
| Zeitschriftentitel: | Stroke |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Stroke, 28, 1997, 1, S. 155-162 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Ovid Technologies (Wolters Kluwer Health)
|
| Schlagwörter: |
| author_facet |
Wang, Xinkang Barone, Frank C. Aiyar, Nambi V. Feuerstein, Giora Z. Wang, Xinkang Barone, Frank C. Aiyar, Nambi V. Feuerstein, Giora Z. |
|---|---|
| author |
Wang, Xinkang Barone, Frank C. Aiyar, Nambi V. Feuerstein, Giora Z. |
| spellingShingle |
Wang, Xinkang Barone, Frank C. Aiyar, Nambi V. Feuerstein, Giora Z. Stroke Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats Advanced and Specialized Nursing Cardiology and Cardiovascular Medicine Neurology (clinical) |
| author_sort |
wang, xinkang |
| spelling |
Wang, Xinkang Barone, Frank C. Aiyar, Nambi V. Feuerstein, Giora Z. 0039-2499 1524-4628 Ovid Technologies (Wolters Kluwer Health) Advanced and Specialized Nursing Cardiology and Cardiovascular Medicine Neurology (clinical) http://dx.doi.org/10.1161/01.str.28.1.155 <jats:p><jats:italic>Background and Purpose</jats:italic>The expression of interleukin-1β (IL-1β) is upregulated after focal brain ischemia, and previous work has demonstrated its involvement in ischemic injury. The IL-1 receptor antagonist (IL-1ra), a natural competitive antagonist of IL-1 receptors (IL-1Rs), has been demonstrated to play a role in attenuating brain ischemic injury. To hypothesize the involvement of the IL-1 system in ischemic injury, we examined other IL-1 components, including IL-1ra, IL-1RI, and IL-1RII for their mRNA expression after focal stroke.</jats:p><jats:p><jats:italic>Methods</jats:italic>Quantitative reverse transcription and polymerase chain reaction (RT-PCR) technique was used to examine the mRNA expression profile of IL-1ra and two IL-1R isoforms in a temporal fashion (n=4 for each time point) after permanent occlusion of the middle cerebral artery (MCAO) in spontaneously hypertensive rats. IL-1ra and IL-1R mRNA expression was confirmed by Northern blot analysis using poly(A) RNA isolated after 2 and 12 hours of MCAO.</jats:p><jats:p><jats:italic>Results</jats:italic>Very low levels of IL-1ra mRNA were detected in sham-operated or nonischemic cortex. IL-1ra mRNA in ischemic cortex was greatly increased at 12 hours (16.5-fold increase over sham samples,<jats:italic>P</jats:italic><.001) and remained elevated for up to 5 days (17.2-fold increase,<jats:italic>P</jats:italic><.01) after MCAO. IL-1RI mRNA was relatively highly expressed in normal cortex and was further elevated late after ischemic injury (3.3-fold increase at day 5,<jats:italic>P</jats:italic><.001). In contrast, the low basal expression of IL-1RII mRNA was remarkably elevated at 6 hours (5.3-fold increase,<jats:italic>P</jats:italic><.05), reaching peak levels 12 hours (10.3-fold increase,<jats:italic>P</jats:italic><.001) after MCAO.</jats:p><jats:p><jats:italic>Conclusions</jats:italic>Differential expression of IL-1β, IL-1ra, IL-1RI, and IL-1RII mRNAs after focal stroke may suggest a distinct role(s) for each component of the IL-1 system in ischemic injury. The data also stress the importance of evaluating all the components of a given cytokine system (eg, agonist, receptors, and natural antagonist) after focal stroke.</jats:p> Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats Stroke |
| doi_str_mv |
10.1161/01.str.28.1.155 |
| facet_avail |
Online Free |
| finc_class_facet |
Medizin |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE2MS8wMS5zdHIuMjguMS4xNTU |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE2MS8wMS5zdHIuMjguMS4xNTU |
| institution |
DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 |
| imprint |
Ovid Technologies (Wolters Kluwer Health), 1997 |
| imprint_str_mv |
Ovid Technologies (Wolters Kluwer Health), 1997 |
| issn |
0039-2499 1524-4628 |
| issn_str_mv |
0039-2499 1524-4628 |
| language |
English |
| mega_collection |
Ovid Technologies (Wolters Kluwer Health) (CrossRef) |
| match_str |
wang1997interleukin1receptorandreceptorantagonistgeneexpressionafterfocalstrokeinrats |
| publishDateSort |
1997 |
| publisher |
Ovid Technologies (Wolters Kluwer Health) |
| recordtype |
ai |
| record_format |
ai |
| series |
Stroke |
| source_id |
49 |
| title |
Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats |
| title_unstemmed |
Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats |
| title_full |
Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats |
| title_fullStr |
Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats |
| title_full_unstemmed |
Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats |
| title_short |
Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats |
| title_sort |
interleukin-1 receptor and receptor antagonist gene expression after focal stroke in rats |
| topic |
Advanced and Specialized Nursing Cardiology and Cardiovascular Medicine Neurology (clinical) |
| url |
http://dx.doi.org/10.1161/01.str.28.1.155 |
| publishDate |
1997 |
| physical |
155-162 |
| description |
<jats:p><jats:italic>Background and Purpose</jats:italic>The expression of interleukin-1β (IL-1β) is upregulated after focal brain ischemia, and previous work has demonstrated its involvement in ischemic injury. The IL-1 receptor antagonist (IL-1ra), a natural competitive antagonist of IL-1 receptors (IL-1Rs), has been demonstrated to play a role in attenuating brain ischemic injury. To hypothesize the involvement of the IL-1 system in ischemic injury, we examined other IL-1 components, including IL-1ra, IL-1RI, and IL-1RII for their mRNA expression after focal stroke.</jats:p><jats:p><jats:italic>Methods</jats:italic>Quantitative reverse transcription and polymerase chain reaction (RT-PCR) technique was used to examine the mRNA expression profile of IL-1ra and two IL-1R isoforms in a temporal fashion (n=4 for each time point) after permanent occlusion of the middle cerebral artery (MCAO) in spontaneously hypertensive rats. IL-1ra and IL-1R mRNA expression was confirmed by Northern blot analysis using poly(A) RNA isolated after 2 and 12 hours of MCAO.</jats:p><jats:p><jats:italic>Results</jats:italic>Very low levels of IL-1ra mRNA were detected in sham-operated or nonischemic cortex. IL-1ra mRNA in ischemic cortex was greatly increased at 12 hours (16.5-fold increase over sham samples,<jats:italic>P</jats:italic><.001) and remained elevated for up to 5 days (17.2-fold increase,<jats:italic>P</jats:italic><.01) after MCAO. IL-1RI mRNA was relatively highly expressed in normal cortex and was further elevated late after ischemic injury (3.3-fold increase at day 5,<jats:italic>P</jats:italic><.001). In contrast, the low basal expression of IL-1RII mRNA was remarkably elevated at 6 hours (5.3-fold increase,<jats:italic>P</jats:italic><.05), reaching peak levels 12 hours (10.3-fold increase,<jats:italic>P</jats:italic><.001) after MCAO.</jats:p><jats:p><jats:italic>Conclusions</jats:italic>Differential expression of IL-1β, IL-1ra, IL-1RI, and IL-1RII mRNAs after focal stroke may suggest a distinct role(s) for each component of the IL-1 system in ischemic injury. The data also stress the importance of evaluating all the components of a given cytokine system (eg, agonist, receptors, and natural antagonist) after focal stroke.</jats:p> |
| container_issue |
1 |
| container_start_page |
155 |
| container_title |
Stroke |
| container_volume |
28 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792341779362611205 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T16:25:19.659Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Interleukin-1+Receptor+and+Receptor+Antagonist+Gene+Expression+After+Focal+Stroke+in+Rats&rft.date=1997-01-01&genre=article&issn=1524-4628&volume=28&issue=1&spage=155&epage=162&pages=155-162&jtitle=Stroke&atitle=Interleukin-1+Receptor+and+Receptor+Antagonist+Gene+Expression+After+Focal+Stroke+in+Rats&aulast=Feuerstein&aufirst=Giora+Z.&rft_id=info%3Adoi%2F10.1161%2F01.str.28.1.155&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792341779362611205 |
| author | Wang, Xinkang, Barone, Frank C., Aiyar, Nambi V., Feuerstein, Giora Z. |
| author_facet | Wang, Xinkang, Barone, Frank C., Aiyar, Nambi V., Feuerstein, Giora Z., Wang, Xinkang, Barone, Frank C., Aiyar, Nambi V., Feuerstein, Giora Z. |
| author_sort | wang, xinkang |
| container_issue | 1 |
| container_start_page | 155 |
| container_title | Stroke |
| container_volume | 28 |
| description | <jats:p><jats:italic>Background and Purpose</jats:italic>The expression of interleukin-1β (IL-1β) is upregulated after focal brain ischemia, and previous work has demonstrated its involvement in ischemic injury. The IL-1 receptor antagonist (IL-1ra), a natural competitive antagonist of IL-1 receptors (IL-1Rs), has been demonstrated to play a role in attenuating brain ischemic injury. To hypothesize the involvement of the IL-1 system in ischemic injury, we examined other IL-1 components, including IL-1ra, IL-1RI, and IL-1RII for their mRNA expression after focal stroke.</jats:p><jats:p><jats:italic>Methods</jats:italic>Quantitative reverse transcription and polymerase chain reaction (RT-PCR) technique was used to examine the mRNA expression profile of IL-1ra and two IL-1R isoforms in a temporal fashion (n=4 for each time point) after permanent occlusion of the middle cerebral artery (MCAO) in spontaneously hypertensive rats. IL-1ra and IL-1R mRNA expression was confirmed by Northern blot analysis using poly(A) RNA isolated after 2 and 12 hours of MCAO.</jats:p><jats:p><jats:italic>Results</jats:italic>Very low levels of IL-1ra mRNA were detected in sham-operated or nonischemic cortex. IL-1ra mRNA in ischemic cortex was greatly increased at 12 hours (16.5-fold increase over sham samples,<jats:italic>P</jats:italic><.001) and remained elevated for up to 5 days (17.2-fold increase,<jats:italic>P</jats:italic><.01) after MCAO. IL-1RI mRNA was relatively highly expressed in normal cortex and was further elevated late after ischemic injury (3.3-fold increase at day 5,<jats:italic>P</jats:italic><.001). In contrast, the low basal expression of IL-1RII mRNA was remarkably elevated at 6 hours (5.3-fold increase,<jats:italic>P</jats:italic><.05), reaching peak levels 12 hours (10.3-fold increase,<jats:italic>P</jats:italic><.001) after MCAO.</jats:p><jats:p><jats:italic>Conclusions</jats:italic>Differential expression of IL-1β, IL-1ra, IL-1RI, and IL-1RII mRNAs after focal stroke may suggest a distinct role(s) for each component of the IL-1 system in ischemic injury. The data also stress the importance of evaluating all the components of a given cytokine system (eg, agonist, receptors, and natural antagonist) after focal stroke.</jats:p> |
| doi_str_mv | 10.1161/01.str.28.1.155 |
| facet_avail | Online, Free |
| finc_class_facet | Medizin |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE2MS8wMS5zdHIuMjguMS4xNTU |
| imprint | Ovid Technologies (Wolters Kluwer Health), 1997 |
| imprint_str_mv | Ovid Technologies (Wolters Kluwer Health), 1997 |
| institution | DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4 |
| issn | 0039-2499, 1524-4628 |
| issn_str_mv | 0039-2499, 1524-4628 |
| language | English |
| last_indexed | 2024-03-01T16:25:19.659Z |
| match_str | wang1997interleukin1receptorandreceptorantagonistgeneexpressionafterfocalstrokeinrats |
| mega_collection | Ovid Technologies (Wolters Kluwer Health) (CrossRef) |
| physical | 155-162 |
| publishDate | 1997 |
| publishDateSort | 1997 |
| publisher | Ovid Technologies (Wolters Kluwer Health) |
| record_format | ai |
| recordtype | ai |
| series | Stroke |
| source_id | 49 |
| spelling | Wang, Xinkang Barone, Frank C. Aiyar, Nambi V. Feuerstein, Giora Z. 0039-2499 1524-4628 Ovid Technologies (Wolters Kluwer Health) Advanced and Specialized Nursing Cardiology and Cardiovascular Medicine Neurology (clinical) http://dx.doi.org/10.1161/01.str.28.1.155 <jats:p><jats:italic>Background and Purpose</jats:italic>The expression of interleukin-1β (IL-1β) is upregulated after focal brain ischemia, and previous work has demonstrated its involvement in ischemic injury. The IL-1 receptor antagonist (IL-1ra), a natural competitive antagonist of IL-1 receptors (IL-1Rs), has been demonstrated to play a role in attenuating brain ischemic injury. To hypothesize the involvement of the IL-1 system in ischemic injury, we examined other IL-1 components, including IL-1ra, IL-1RI, and IL-1RII for their mRNA expression after focal stroke.</jats:p><jats:p><jats:italic>Methods</jats:italic>Quantitative reverse transcription and polymerase chain reaction (RT-PCR) technique was used to examine the mRNA expression profile of IL-1ra and two IL-1R isoforms in a temporal fashion (n=4 for each time point) after permanent occlusion of the middle cerebral artery (MCAO) in spontaneously hypertensive rats. IL-1ra and IL-1R mRNA expression was confirmed by Northern blot analysis using poly(A) RNA isolated after 2 and 12 hours of MCAO.</jats:p><jats:p><jats:italic>Results</jats:italic>Very low levels of IL-1ra mRNA were detected in sham-operated or nonischemic cortex. IL-1ra mRNA in ischemic cortex was greatly increased at 12 hours (16.5-fold increase over sham samples,<jats:italic>P</jats:italic><.001) and remained elevated for up to 5 days (17.2-fold increase,<jats:italic>P</jats:italic><.01) after MCAO. IL-1RI mRNA was relatively highly expressed in normal cortex and was further elevated late after ischemic injury (3.3-fold increase at day 5,<jats:italic>P</jats:italic><.001). In contrast, the low basal expression of IL-1RII mRNA was remarkably elevated at 6 hours (5.3-fold increase,<jats:italic>P</jats:italic><.05), reaching peak levels 12 hours (10.3-fold increase,<jats:italic>P</jats:italic><.001) after MCAO.</jats:p><jats:p><jats:italic>Conclusions</jats:italic>Differential expression of IL-1β, IL-1ra, IL-1RI, and IL-1RII mRNAs after focal stroke may suggest a distinct role(s) for each component of the IL-1 system in ischemic injury. The data also stress the importance of evaluating all the components of a given cytokine system (eg, agonist, receptors, and natural antagonist) after focal stroke.</jats:p> Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats Stroke |
| spellingShingle | Wang, Xinkang, Barone, Frank C., Aiyar, Nambi V., Feuerstein, Giora Z., Stroke, Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats, Advanced and Specialized Nursing, Cardiology and Cardiovascular Medicine, Neurology (clinical) |
| title | Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats |
| title_full | Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats |
| title_fullStr | Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats |
| title_full_unstemmed | Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats |
| title_short | Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats |
| title_sort | interleukin-1 receptor and receptor antagonist gene expression after focal stroke in rats |
| title_unstemmed | Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats |
| topic | Advanced and Specialized Nursing, Cardiology and Cardiovascular Medicine, Neurology (clinical) |
| url | http://dx.doi.org/10.1161/01.str.28.1.155 |