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Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant
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| Zeitschriftentitel: | Kidney and Blood Pressure Research |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Kidney and Blood Pressure Research, 17, 1994, 1, S. 27-39 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
S. Karger AG
|
| Schlagwörter: |
| author_facet |
Dijkstra, S. Lohrmann, E. Van Kerkhove, E. Greger, R. Dijkstra, S. Lohrmann, E. Van Kerkhove, E. Greger, R. |
|---|---|
| author |
Dijkstra, S. Lohrmann, E. Van Kerkhove, E. Greger, R. |
| spellingShingle |
Dijkstra, S. Lohrmann, E. Van Kerkhove, E. Greger, R. Kidney and Blood Pressure Research Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant Cardiology and Cardiovascular Medicine Nephrology Cardiology and Cardiovascular Medicine Nephrology |
| author_sort |
dijkstra, s. |
| spelling |
Dijkstra, S. Lohrmann, E. Van Kerkhove, E. Greger, R. 1420-4096 1423-0143 S. Karger AG Cardiology and Cardiovascular Medicine Nephrology Cardiology and Cardiovascular Medicine Nephrology http://dx.doi.org/10.1159/000173785 <jats:p>The active pump mechanisms involved in K<sup>+</sup> secretion of the malpighian tubules of the ant and present in the luminal membrane were investigated on isolated, luminally perfused tubules of <i>Formica. </i>The specific blocker for vacuolar type ATPases, bafilomycin A<sub>1</sub>, was found to half-maximally inhibit secretion at a concentration of 10<sup>-5</sup> mol/l when added to the lumen. N-Ethylmaleimide reduced the calculated short circuit current (I<sub>sc</sub>) to 78 and 21% of control value when added at 5 · 10<sup>-4</sup> mol/l, respectively, to the lumen and the bath. Reducing luminal pH inhibited I<sub>sc</sub> with a half-maximal inhibition at a luminal pH of 4.5. Acidified omeprazole, Schering compound 28080 and vanadate (both 10<sup>-3</sup> and 10<sup>-4</sup> mol/l) inhibited I<sub>sc</sub> only partially. The present data suggest that the luminal membrane of ant malpighian tubules contains a H<sup>+</sup> pump. This pump is only poorly bafilomycin-sensitive. Furthermore, additional active transport systems responsible for secretion may be present. Part of these results have been published as abstracts.</jats:p> Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant Kidney and Blood Pressure Research |
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1994 |
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49 |
| title |
Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant |
| title_unstemmed |
Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant |
| title_full |
Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant |
| title_fullStr |
Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant |
| title_full_unstemmed |
Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant |
| title_short |
Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant |
| title_sort |
characteristics of the luminal proton pump in malpighian tubules of the ant |
| topic |
Cardiology and Cardiovascular Medicine Nephrology Cardiology and Cardiovascular Medicine Nephrology |
| url |
http://dx.doi.org/10.1159/000173785 |
| publishDate |
1994 |
| physical |
27-39 |
| description |
<jats:p>The active pump mechanisms involved in K<sup>+</sup> secretion of the malpighian tubules of the ant and present in the luminal membrane were investigated on isolated, luminally perfused tubules of <i>Formica. </i>The specific blocker for vacuolar type ATPases, bafilomycin A<sub>1</sub>, was found to half-maximally inhibit secretion at a concentration of 10<sup>-5</sup> mol/l when added to the lumen. N-Ethylmaleimide reduced the calculated short circuit current (I<sub>sc</sub>) to 78 and 21% of control value when added at 5 · 10<sup>-4</sup> mol/l, respectively, to the lumen and the bath. Reducing luminal pH inhibited I<sub>sc</sub> with a half-maximal inhibition at a luminal pH of 4.5. Acidified omeprazole, Schering compound 28080 and vanadate (both 10<sup>-3</sup> and 10<sup>-4</sup> mol/l) inhibited I<sub>sc</sub> only partially. The present data suggest that the luminal membrane of ant malpighian tubules contains a H<sup>+</sup> pump. This pump is only poorly bafilomycin-sensitive. Furthermore, additional active transport systems responsible for secretion may be present. Part of these results have been published as abstracts.</jats:p> |
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| author | Dijkstra, S., Lohrmann, E., Van Kerkhove, E., Greger, R. |
| author_facet | Dijkstra, S., Lohrmann, E., Van Kerkhove, E., Greger, R., Dijkstra, S., Lohrmann, E., Van Kerkhove, E., Greger, R. |
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| description | <jats:p>The active pump mechanisms involved in K<sup>+</sup> secretion of the malpighian tubules of the ant and present in the luminal membrane were investigated on isolated, luminally perfused tubules of <i>Formica. </i>The specific blocker for vacuolar type ATPases, bafilomycin A<sub>1</sub>, was found to half-maximally inhibit secretion at a concentration of 10<sup>-5</sup> mol/l when added to the lumen. N-Ethylmaleimide reduced the calculated short circuit current (I<sub>sc</sub>) to 78 and 21% of control value when added at 5 · 10<sup>-4</sup> mol/l, respectively, to the lumen and the bath. Reducing luminal pH inhibited I<sub>sc</sub> with a half-maximal inhibition at a luminal pH of 4.5. Acidified omeprazole, Schering compound 28080 and vanadate (both 10<sup>-3</sup> and 10<sup>-4</sup> mol/l) inhibited I<sub>sc</sub> only partially. The present data suggest that the luminal membrane of ant malpighian tubules contains a H<sup>+</sup> pump. This pump is only poorly bafilomycin-sensitive. Furthermore, additional active transport systems responsible for secretion may be present. Part of these results have been published as abstracts.</jats:p> |
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| imprint | S. Karger AG, 1994 |
| imprint_str_mv | S. Karger AG, 1994 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229 |
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| physical | 27-39 |
| publishDate | 1994 |
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| series | Kidney and Blood Pressure Research |
| source_id | 49 |
| spelling | Dijkstra, S. Lohrmann, E. Van Kerkhove, E. Greger, R. 1420-4096 1423-0143 S. Karger AG Cardiology and Cardiovascular Medicine Nephrology Cardiology and Cardiovascular Medicine Nephrology http://dx.doi.org/10.1159/000173785 <jats:p>The active pump mechanisms involved in K<sup>+</sup> secretion of the malpighian tubules of the ant and present in the luminal membrane were investigated on isolated, luminally perfused tubules of <i>Formica. </i>The specific blocker for vacuolar type ATPases, bafilomycin A<sub>1</sub>, was found to half-maximally inhibit secretion at a concentration of 10<sup>-5</sup> mol/l when added to the lumen. N-Ethylmaleimide reduced the calculated short circuit current (I<sub>sc</sub>) to 78 and 21% of control value when added at 5 · 10<sup>-4</sup> mol/l, respectively, to the lumen and the bath. Reducing luminal pH inhibited I<sub>sc</sub> with a half-maximal inhibition at a luminal pH of 4.5. Acidified omeprazole, Schering compound 28080 and vanadate (both 10<sup>-3</sup> and 10<sup>-4</sup> mol/l) inhibited I<sub>sc</sub> only partially. The present data suggest that the luminal membrane of ant malpighian tubules contains a H<sup>+</sup> pump. This pump is only poorly bafilomycin-sensitive. Furthermore, additional active transport systems responsible for secretion may be present. Part of these results have been published as abstracts.</jats:p> Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant Kidney and Blood Pressure Research |
| spellingShingle | Dijkstra, S., Lohrmann, E., Van Kerkhove, E., Greger, R., Kidney and Blood Pressure Research, Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant, Cardiology and Cardiovascular Medicine, Nephrology, Cardiology and Cardiovascular Medicine, Nephrology |
| title | Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant |
| title_full | Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant |
| title_fullStr | Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant |
| title_full_unstemmed | Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant |
| title_short | Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant |
| title_sort | characteristics of the luminal proton pump in malpighian tubules of the ant |
| title_unstemmed | Characteristics of the Luminal Proton Pump in Malpighian Tubules of the Ant |
| topic | Cardiology and Cardiovascular Medicine, Nephrology, Cardiology and Cardiovascular Medicine, Nephrology |
| url | http://dx.doi.org/10.1159/000173785 |