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Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood
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| Zeitschriftentitel: | Medical Principles and Practice |
|---|---|
| Personen und Körperschaften: | , |
| In: | Medical Principles and Practice, 28, 2019, 4, S. 361-366 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
S. Karger AG
|
| Schlagwörter: |
| author_facet |
Al-Faris, Lama Al-Humood, Salah Al-Faris, Lama Al-Humood, Salah |
|---|---|
| author |
Al-Faris, Lama Al-Humood, Salah |
| spellingShingle |
Al-Faris, Lama Al-Humood, Salah Medical Principles and Practice Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood General Medicine |
| author_sort |
al-faris, lama |
| spelling |
Al-Faris, Lama Al-Humood, Salah 1011-7571 1423-0151 S. Karger AG General Medicine http://dx.doi.org/10.1159/000499428 <jats:p><b><i>Objective:</i></b> Red blood cells (RBCs) in storage undergo structural and biochemical changes that may cause functional effects. Studies exploring structural changes affecting the expression levels of CD55 and CD59 on RBCs are limited. The aim of this study was to investigate the pattern of CD55 and CD59 expression on RBCs in stored blood from Arab donors. <b><i>Materials and Methods:</i></b> Flow-cytometric analysis was performed on RBCs from 92 packed RBC (PRBC) units, stored for varying times, and from 56 nonstored RBC from healthy controls using the commercial REDQUANT kit. <b><i>Results:</i></b> The proportions of CD55- and CD59-deficient RBCs from stored PRBC units did not significantly differ when compared with those from healthy controls; however, the mean fluorescent intensity (MFI) of CD59 expression, but not MFI of CD55 expression, on RBCs from stored PRBC units was significantly reduced when compared to the expression of RBCs from healthy controls (<i>p</i> = 0.02). MFI of CD55 expression on RBCs from PRBC units did not significantly differ among the 3 groups of stored RBC; however, there was a statistically significant time-dependent preferential decline in MFI of CD59 expression on RBCs from stored PRBC units (<i>p</i> &#x3c; 0.01). <b><i>Conclusion:</i></b> There is a preferential time-dependent decline in the expression of CD59, but not of CD55, on stored RBCs, the in vivo significance of which in relation to the response to PRBC transfusion needs further investigation.</jats:p> Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood Medical Principles and Practice |
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10.1159/000499428 |
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S. Karger AG, 2019 |
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S. Karger AG, 2019 |
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1011-7571 1423-0151 |
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2019 |
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Medical Principles and Practice |
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49 |
| title |
Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood |
| title_unstemmed |
Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood |
| title_full |
Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood |
| title_fullStr |
Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood |
| title_full_unstemmed |
Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood |
| title_short |
Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood |
| title_sort |
altered expression levels of cd59, but not cd55, on red blood cells in stored blood |
| topic |
General Medicine |
| url |
http://dx.doi.org/10.1159/000499428 |
| publishDate |
2019 |
| physical |
361-366 |
| description |
<jats:p><b><i>Objective:</i></b> Red blood cells (RBCs) in storage undergo structural and biochemical changes that may cause functional effects. Studies exploring structural changes affecting the expression levels of CD55 and CD59 on RBCs are limited. The aim of this study was to investigate the pattern of CD55 and CD59 expression on RBCs in stored blood from Arab donors. <b><i>Materials and Methods:</i></b> Flow-cytometric analysis was performed on RBCs from 92 packed RBC (PRBC) units, stored for varying times, and from 56 nonstored RBC from healthy controls using the commercial REDQUANT kit. <b><i>Results:</i></b> The proportions of CD55- and CD59-deficient RBCs from stored PRBC units did not significantly differ when compared with those from healthy controls; however, the mean fluorescent intensity (MFI) of CD59 expression, but not MFI of CD55 expression, on RBCs from stored PRBC units was significantly reduced when compared to the expression of RBCs from healthy controls (<i>p</i> = 0.02). MFI of CD55 expression on RBCs from PRBC units did not significantly differ among the 3 groups of stored RBC; however, there was a statistically significant time-dependent preferential decline in MFI of CD59 expression on RBCs from stored PRBC units (<i>p</i> &#x3c; 0.01). <b><i>Conclusion:</i></b> There is a preferential time-dependent decline in the expression of CD59, but not of CD55, on stored RBCs, the in vivo significance of which in relation to the response to PRBC transfusion needs further investigation.</jats:p> |
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| author | Al-Faris, Lama, Al-Humood, Salah |
| author_facet | Al-Faris, Lama, Al-Humood, Salah, Al-Faris, Lama, Al-Humood, Salah |
| author_sort | al-faris, lama |
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| container_title | Medical Principles and Practice |
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| description | <jats:p><b><i>Objective:</i></b> Red blood cells (RBCs) in storage undergo structural and biochemical changes that may cause functional effects. Studies exploring structural changes affecting the expression levels of CD55 and CD59 on RBCs are limited. The aim of this study was to investigate the pattern of CD55 and CD59 expression on RBCs in stored blood from Arab donors. <b><i>Materials and Methods:</i></b> Flow-cytometric analysis was performed on RBCs from 92 packed RBC (PRBC) units, stored for varying times, and from 56 nonstored RBC from healthy controls using the commercial REDQUANT kit. <b><i>Results:</i></b> The proportions of CD55- and CD59-deficient RBCs from stored PRBC units did not significantly differ when compared with those from healthy controls; however, the mean fluorescent intensity (MFI) of CD59 expression, but not MFI of CD55 expression, on RBCs from stored PRBC units was significantly reduced when compared to the expression of RBCs from healthy controls (<i>p</i> = 0.02). MFI of CD55 expression on RBCs from PRBC units did not significantly differ among the 3 groups of stored RBC; however, there was a statistically significant time-dependent preferential decline in MFI of CD59 expression on RBCs from stored PRBC units (<i>p</i> &#x3c; 0.01). <b><i>Conclusion:</i></b> There is a preferential time-dependent decline in the expression of CD59, but not of CD55, on stored RBCs, the in vivo significance of which in relation to the response to PRBC transfusion needs further investigation.</jats:p> |
| doi_str_mv | 10.1159/000499428 |
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| imprint | S. Karger AG, 2019 |
| imprint_str_mv | S. Karger AG, 2019 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 1011-7571, 1423-0151 |
| issn_str_mv | 1011-7571, 1423-0151 |
| language | English |
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| physical | 361-366 |
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| publishDateSort | 2019 |
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| spelling | Al-Faris, Lama Al-Humood, Salah 1011-7571 1423-0151 S. Karger AG General Medicine http://dx.doi.org/10.1159/000499428 <jats:p><b><i>Objective:</i></b> Red blood cells (RBCs) in storage undergo structural and biochemical changes that may cause functional effects. Studies exploring structural changes affecting the expression levels of CD55 and CD59 on RBCs are limited. The aim of this study was to investigate the pattern of CD55 and CD59 expression on RBCs in stored blood from Arab donors. <b><i>Materials and Methods:</i></b> Flow-cytometric analysis was performed on RBCs from 92 packed RBC (PRBC) units, stored for varying times, and from 56 nonstored RBC from healthy controls using the commercial REDQUANT kit. <b><i>Results:</i></b> The proportions of CD55- and CD59-deficient RBCs from stored PRBC units did not significantly differ when compared with those from healthy controls; however, the mean fluorescent intensity (MFI) of CD59 expression, but not MFI of CD55 expression, on RBCs from stored PRBC units was significantly reduced when compared to the expression of RBCs from healthy controls (<i>p</i> = 0.02). MFI of CD55 expression on RBCs from PRBC units did not significantly differ among the 3 groups of stored RBC; however, there was a statistically significant time-dependent preferential decline in MFI of CD59 expression on RBCs from stored PRBC units (<i>p</i> &#x3c; 0.01). <b><i>Conclusion:</i></b> There is a preferential time-dependent decline in the expression of CD59, but not of CD55, on stored RBCs, the in vivo significance of which in relation to the response to PRBC transfusion needs further investigation.</jats:p> Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood Medical Principles and Practice |
| spellingShingle | Al-Faris, Lama, Al-Humood, Salah, Medical Principles and Practice, Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood, General Medicine |
| title | Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood |
| title_full | Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood |
| title_fullStr | Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood |
| title_full_unstemmed | Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood |
| title_short | Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood |
| title_sort | altered expression levels of cd59, but not cd55, on red blood cells in stored blood |
| title_unstemmed | Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood |
| topic | General Medicine |
| url | http://dx.doi.org/10.1159/000499428 |