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Resveratrol-induced apoptotic death in human U251 glioma cells
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Zeitschriftentitel: | Molecular Cancer Therapeutics |
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Personen und Körperschaften: | , , , , , , , , , , , |
In: | Molecular Cancer Therapeutics, 4, 2005, 4, S. 554-561 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
American Association for Cancer Research (AACR)
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Schlagwörter: |
author_facet |
Jiang, Hao Zhang, Lijie Kuo, Jarret Kuo, Kelly Gautam, Subhash C. Groc, Laurent Rodriguez, Alba I. Koubi, David Jackson Hunter, Tangella Corcoran, George B. Seidman, Michael D. Levine, Robert A. Jiang, Hao Zhang, Lijie Kuo, Jarret Kuo, Kelly Gautam, Subhash C. Groc, Laurent Rodriguez, Alba I. Koubi, David Jackson Hunter, Tangella Corcoran, George B. Seidman, Michael D. Levine, Robert A. |
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author |
Jiang, Hao Zhang, Lijie Kuo, Jarret Kuo, Kelly Gautam, Subhash C. Groc, Laurent Rodriguez, Alba I. Koubi, David Jackson Hunter, Tangella Corcoran, George B. Seidman, Michael D. Levine, Robert A. |
spellingShingle |
Jiang, Hao Zhang, Lijie Kuo, Jarret Kuo, Kelly Gautam, Subhash C. Groc, Laurent Rodriguez, Alba I. Koubi, David Jackson Hunter, Tangella Corcoran, George B. Seidman, Michael D. Levine, Robert A. Molecular Cancer Therapeutics Resveratrol-induced apoptotic death in human U251 glioma cells Cancer Research Oncology |
author_sort |
jiang, hao |
spelling |
Jiang, Hao Zhang, Lijie Kuo, Jarret Kuo, Kelly Gautam, Subhash C. Groc, Laurent Rodriguez, Alba I. Koubi, David Jackson Hunter, Tangella Corcoran, George B. Seidman, Michael D. Levine, Robert A. 1535-7163 1538-8514 American Association for Cancer Research (AACR) Cancer Research Oncology http://dx.doi.org/10.1158/1535-7163.mct-04-0056 <jats:title>Abstract</jats:title> <jats:p>Resveratrol (trans-3,4′,5-trihydroxystilbene) is a naturally occurring polyphenolic compound highly enriched in grapes, peanuts, red wine, and a variety of food sources. Resveratrol has antiinflammatory and antioxidant properties, and also has potent anticancer properties. Human glioma U251 cells were used to understand the molecular mechanisms by which resveratrol acts as an anticancer agent, since glioma is a particularly difficult cancer to treat and eradicate. Our data show that resveratrol induces dose- and time-dependent death of U251 cells, as measured by lactate dehydrogenase release and internucleosomal DNA fragmentation assays. Resveratrol induces activation of caspase-3 and increases the cleavage of the downstream caspase substrate, poly(ADP-ribose) polymerase. Resveratrol-induced DNA fragmentation can be completely blocked by either a general caspase inhibitor (Z-VAD-FMK) or a selective caspase-3 inhibitor (Z-DEVD-FMK), but not by a selective caspase-1 inhibitor. Resveratrol induces cytochrome c release from mitochondria to the cytoplasm and activation of caspase-9. Resveratrol also increases expression of proapoptotic Bax and its translocation to the mitochondria. Resveratrol inhibits U251 proliferation, as measured by MTS assay [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt], and induces G0/G1 growth arrest, as determined by flow cytometry. The cyclin-dependent kinase inhibitor, olomoucine, prevents cell cycle progression and resveratrol-induced apoptosis. These results suggest that multiple signaling pathways may underlie the apoptotic death of U251 glioma induced by resveratrol, which warrants further exploration as an anticancer agent in human glioma.</jats:p> Resveratrol-induced apoptotic death in human U251 glioma cells Molecular Cancer Therapeutics |
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10.1158/1535-7163.mct-04-0056 |
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American Association for Cancer Research (AACR), 2005 |
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American Association for Cancer Research (AACR), 2005 |
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1535-7163 1538-8514 |
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2005 |
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American Association for Cancer Research (AACR) |
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Molecular Cancer Therapeutics |
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title |
Resveratrol-induced apoptotic death in human U251 glioma cells |
title_unstemmed |
Resveratrol-induced apoptotic death in human U251 glioma cells |
title_full |
Resveratrol-induced apoptotic death in human U251 glioma cells |
title_fullStr |
Resveratrol-induced apoptotic death in human U251 glioma cells |
title_full_unstemmed |
Resveratrol-induced apoptotic death in human U251 glioma cells |
title_short |
Resveratrol-induced apoptotic death in human U251 glioma cells |
title_sort |
resveratrol-induced apoptotic death in human u251 glioma cells |
topic |
Cancer Research Oncology |
url |
http://dx.doi.org/10.1158/1535-7163.mct-04-0056 |
publishDate |
2005 |
physical |
554-561 |
description |
<jats:title>Abstract</jats:title>
<jats:p>Resveratrol (trans-3,4′,5-trihydroxystilbene) is a naturally occurring polyphenolic compound highly enriched in grapes, peanuts, red wine, and a variety of food sources. Resveratrol has antiinflammatory and antioxidant properties, and also has potent anticancer properties. Human glioma U251 cells were used to understand the molecular mechanisms by which resveratrol acts as an anticancer agent, since glioma is a particularly difficult cancer to treat and eradicate. Our data show that resveratrol induces dose- and time-dependent death of U251 cells, as measured by lactate dehydrogenase release and internucleosomal DNA fragmentation assays. Resveratrol induces activation of caspase-3 and increases the cleavage of the downstream caspase substrate, poly(ADP-ribose) polymerase. Resveratrol-induced DNA fragmentation can be completely blocked by either a general caspase inhibitor (Z-VAD-FMK) or a selective caspase-3 inhibitor (Z-DEVD-FMK), but not by a selective caspase-1 inhibitor. Resveratrol induces cytochrome c release from mitochondria to the cytoplasm and activation of caspase-9. Resveratrol also increases expression of proapoptotic Bax and its translocation to the mitochondria. Resveratrol inhibits U251 proliferation, as measured by MTS assay [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt], and induces G0/G1 growth arrest, as determined by flow cytometry. The cyclin-dependent kinase inhibitor, olomoucine, prevents cell cycle progression and resveratrol-induced apoptosis. These results suggest that multiple signaling pathways may underlie the apoptotic death of U251 glioma induced by resveratrol, which warrants further exploration as an anticancer agent in human glioma.</jats:p> |
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author | Jiang, Hao, Zhang, Lijie, Kuo, Jarret, Kuo, Kelly, Gautam, Subhash C., Groc, Laurent, Rodriguez, Alba I., Koubi, David, Jackson Hunter, Tangella, Corcoran, George B., Seidman, Michael D., Levine, Robert A. |
author_facet | Jiang, Hao, Zhang, Lijie, Kuo, Jarret, Kuo, Kelly, Gautam, Subhash C., Groc, Laurent, Rodriguez, Alba I., Koubi, David, Jackson Hunter, Tangella, Corcoran, George B., Seidman, Michael D., Levine, Robert A., Jiang, Hao, Zhang, Lijie, Kuo, Jarret, Kuo, Kelly, Gautam, Subhash C., Groc, Laurent, Rodriguez, Alba I., Koubi, David, Jackson Hunter, Tangella, Corcoran, George B., Seidman, Michael D., Levine, Robert A. |
author_sort | jiang, hao |
container_issue | 4 |
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description | <jats:title>Abstract</jats:title> <jats:p>Resveratrol (trans-3,4′,5-trihydroxystilbene) is a naturally occurring polyphenolic compound highly enriched in grapes, peanuts, red wine, and a variety of food sources. Resveratrol has antiinflammatory and antioxidant properties, and also has potent anticancer properties. Human glioma U251 cells were used to understand the molecular mechanisms by which resveratrol acts as an anticancer agent, since glioma is a particularly difficult cancer to treat and eradicate. Our data show that resveratrol induces dose- and time-dependent death of U251 cells, as measured by lactate dehydrogenase release and internucleosomal DNA fragmentation assays. Resveratrol induces activation of caspase-3 and increases the cleavage of the downstream caspase substrate, poly(ADP-ribose) polymerase. Resveratrol-induced DNA fragmentation can be completely blocked by either a general caspase inhibitor (Z-VAD-FMK) or a selective caspase-3 inhibitor (Z-DEVD-FMK), but not by a selective caspase-1 inhibitor. Resveratrol induces cytochrome c release from mitochondria to the cytoplasm and activation of caspase-9. Resveratrol also increases expression of proapoptotic Bax and its translocation to the mitochondria. Resveratrol inhibits U251 proliferation, as measured by MTS assay [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt], and induces G0/G1 growth arrest, as determined by flow cytometry. The cyclin-dependent kinase inhibitor, olomoucine, prevents cell cycle progression and resveratrol-induced apoptosis. These results suggest that multiple signaling pathways may underlie the apoptotic death of U251 glioma induced by resveratrol, which warrants further exploration as an anticancer agent in human glioma.</jats:p> |
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spelling | Jiang, Hao Zhang, Lijie Kuo, Jarret Kuo, Kelly Gautam, Subhash C. Groc, Laurent Rodriguez, Alba I. Koubi, David Jackson Hunter, Tangella Corcoran, George B. Seidman, Michael D. Levine, Robert A. 1535-7163 1538-8514 American Association for Cancer Research (AACR) Cancer Research Oncology http://dx.doi.org/10.1158/1535-7163.mct-04-0056 <jats:title>Abstract</jats:title> <jats:p>Resveratrol (trans-3,4′,5-trihydroxystilbene) is a naturally occurring polyphenolic compound highly enriched in grapes, peanuts, red wine, and a variety of food sources. Resveratrol has antiinflammatory and antioxidant properties, and also has potent anticancer properties. Human glioma U251 cells were used to understand the molecular mechanisms by which resveratrol acts as an anticancer agent, since glioma is a particularly difficult cancer to treat and eradicate. Our data show that resveratrol induces dose- and time-dependent death of U251 cells, as measured by lactate dehydrogenase release and internucleosomal DNA fragmentation assays. Resveratrol induces activation of caspase-3 and increases the cleavage of the downstream caspase substrate, poly(ADP-ribose) polymerase. Resveratrol-induced DNA fragmentation can be completely blocked by either a general caspase inhibitor (Z-VAD-FMK) or a selective caspase-3 inhibitor (Z-DEVD-FMK), but not by a selective caspase-1 inhibitor. Resveratrol induces cytochrome c release from mitochondria to the cytoplasm and activation of caspase-9. Resveratrol also increases expression of proapoptotic Bax and its translocation to the mitochondria. Resveratrol inhibits U251 proliferation, as measured by MTS assay [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt], and induces G0/G1 growth arrest, as determined by flow cytometry. The cyclin-dependent kinase inhibitor, olomoucine, prevents cell cycle progression and resveratrol-induced apoptosis. These results suggest that multiple signaling pathways may underlie the apoptotic death of U251 glioma induced by resveratrol, which warrants further exploration as an anticancer agent in human glioma.</jats:p> Resveratrol-induced apoptotic death in human U251 glioma cells Molecular Cancer Therapeutics |
spellingShingle | Jiang, Hao, Zhang, Lijie, Kuo, Jarret, Kuo, Kelly, Gautam, Subhash C., Groc, Laurent, Rodriguez, Alba I., Koubi, David, Jackson Hunter, Tangella, Corcoran, George B., Seidman, Michael D., Levine, Robert A., Molecular Cancer Therapeutics, Resveratrol-induced apoptotic death in human U251 glioma cells, Cancer Research, Oncology |
title | Resveratrol-induced apoptotic death in human U251 glioma cells |
title_full | Resveratrol-induced apoptotic death in human U251 glioma cells |
title_fullStr | Resveratrol-induced apoptotic death in human U251 glioma cells |
title_full_unstemmed | Resveratrol-induced apoptotic death in human U251 glioma cells |
title_short | Resveratrol-induced apoptotic death in human U251 glioma cells |
title_sort | resveratrol-induced apoptotic death in human u251 glioma cells |
title_unstemmed | Resveratrol-induced apoptotic death in human U251 glioma cells |
topic | Cancer Research, Oncology |
url | http://dx.doi.org/10.1158/1535-7163.mct-04-0056 |