Eintrag weiter verarbeiten
Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features
Gespeichert in:
| Zeitschriftentitel: | International Journal of Polymer Science |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | International Journal of Polymer Science, 2017, 2017, S. 1-8 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Hindawi Limited
|
| Schlagwörter: |
| author_facet |
Dimida, Simona Barca, Amilcare Cancelli, Nadia De Benedictis, Vincenzo Raucci, Maria Grazia Demitri, Christian Dimida, Simona Barca, Amilcare Cancelli, Nadia De Benedictis, Vincenzo Raucci, Maria Grazia Demitri, Christian |
|---|---|
| author |
Dimida, Simona Barca, Amilcare Cancelli, Nadia De Benedictis, Vincenzo Raucci, Maria Grazia Demitri, Christian |
| spellingShingle |
Dimida, Simona Barca, Amilcare Cancelli, Nadia De Benedictis, Vincenzo Raucci, Maria Grazia Demitri, Christian International Journal of Polymer Science Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features Polymers and Plastics |
| author_sort |
dimida, simona |
| spelling |
Dimida, Simona Barca, Amilcare Cancelli, Nadia De Benedictis, Vincenzo Raucci, Maria Grazia Demitri, Christian 1687-9422 1687-9430 Hindawi Limited Polymers and Plastics http://dx.doi.org/10.1155/2017/8410750 <jats:p>Genipin (GN) is a natural molecule extracted from the fruit of<jats:italic> Gardenia jasminoides</jats:italic> Ellis according to modern microbiological processes. Genipin is considered as a favorable cross-linking agent due to its low cytotoxicity compared to widely used cross-linkers; it cross-links compounds with primary amine groups such as proteins, collagen, and chitosan. Chitosan is a biocompatible polymer that is currently studied in bone tissue engineering for its capacity to promote growth and mineral-rich matrix deposition by osteoblasts in culture. In this work, two genipin cross-linked chitosan scaffolds for bone repair and regeneration were prepared with different GN concentrations, and their chemical, physical, and biological properties were explored. Scanning electron microscopy and mechanical tests revealed that nonremarkable changes in morphology, porosity, and mechanical strength of scaffolds are induced by increasing the cross-linking degree. Also, the degradation rate was shown to decrease while increasing the cross-linking degree, with the high cross-linking density of the scaffold disabling the hydrolysis activity. Finally, basic biocompatibility was investigated in vitro, by evaluating proliferation of two human-derived cell lines, namely, the MG63 (human immortalized osteosarcoma) and the hMSCs (human mesenchymal stem cells), as suitable cell models for bone tissue engineering applications of biomaterials.</jats:p> Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features International Journal of Polymer Science |
| doi_str_mv |
10.1155/2017/8410750 |
| facet_avail |
Online Free |
| finc_class_facet |
Chemie und Pharmazie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1NS8yMDE3Lzg0MTA3NTA |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1NS8yMDE3Lzg0MTA3NTA |
| institution |
DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Zwi2 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 |
| imprint |
Hindawi Limited, 2017 |
| imprint_str_mv |
Hindawi Limited, 2017 |
| issn |
1687-9430 1687-9422 |
| issn_str_mv |
1687-9430 1687-9422 |
| language |
English |
| mega_collection |
Hindawi Limited (CrossRef) |
| match_str |
dimida2017effectsofgenipinconcentrationoncrosslinkedchitosanscaffoldsforbonetissueengineeringstructuralcharacterizationandevidenceofbiocompatibilityfeatures |
| publishDateSort |
2017 |
| publisher |
Hindawi Limited |
| recordtype |
ai |
| record_format |
ai |
| series |
International Journal of Polymer Science |
| source_id |
49 |
| title |
Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features |
| title_unstemmed |
Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features |
| title_full |
Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features |
| title_fullStr |
Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features |
| title_full_unstemmed |
Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features |
| title_short |
Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features |
| title_sort |
effects of genipin concentration on cross-linked chitosan scaffolds for bone tissue engineering: structural characterization and evidence of biocompatibility features |
| topic |
Polymers and Plastics |
| url |
http://dx.doi.org/10.1155/2017/8410750 |
| publishDate |
2017 |
| physical |
1-8 |
| description |
<jats:p>Genipin (GN) is a natural molecule extracted from the fruit of<jats:italic> Gardenia jasminoides</jats:italic> Ellis according to modern microbiological processes. Genipin is considered as a favorable cross-linking agent due to its low cytotoxicity compared to widely used cross-linkers; it cross-links compounds with primary amine groups such as proteins, collagen, and chitosan. Chitosan is a biocompatible polymer that is currently studied in bone tissue engineering for its capacity to promote growth and mineral-rich matrix deposition by osteoblasts in culture. In this work, two genipin cross-linked chitosan scaffolds for bone repair and regeneration were prepared with different GN concentrations, and their chemical, physical, and biological properties were explored. Scanning electron microscopy and mechanical tests revealed that nonremarkable changes in morphology, porosity, and mechanical strength of scaffolds are induced by increasing the cross-linking degree. Also, the degradation rate was shown to decrease while increasing the cross-linking degree, with the high cross-linking density of the scaffold disabling the hydrolysis activity. Finally, basic biocompatibility was investigated in vitro, by evaluating proliferation of two human-derived cell lines, namely, the MG63 (human immortalized osteosarcoma) and the hMSCs (human mesenchymal stem cells), as suitable cell models for bone tissue engineering applications of biomaterials.</jats:p> |
| container_start_page |
1 |
| container_title |
International Journal of Polymer Science |
| container_volume |
2017 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792348005899173897 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T18:04:03.243Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Effects+of+Genipin+Concentration+on+Cross-Linked+Chitosan+Scaffolds+for+Bone+Tissue+Engineering%3A+Structural+Characterization+and+Evidence+of+Biocompatibility+Features&rft.date=2017-01-01&genre=article&issn=1687-9430&volume=2017&spage=1&epage=8&pages=1-8&jtitle=International+Journal+of+Polymer+Science&atitle=Effects+of+Genipin+Concentration+on+Cross-Linked+Chitosan+Scaffolds+for+Bone+Tissue+Engineering%3A+Structural+Characterization+and+Evidence+of+Biocompatibility+Features&aulast=Demitri&aufirst=Christian&rft_id=info%3Adoi%2F10.1155%2F2017%2F8410750&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792348005899173897 |
| author | Dimida, Simona, Barca, Amilcare, Cancelli, Nadia, De Benedictis, Vincenzo, Raucci, Maria Grazia, Demitri, Christian |
| author_facet | Dimida, Simona, Barca, Amilcare, Cancelli, Nadia, De Benedictis, Vincenzo, Raucci, Maria Grazia, Demitri, Christian, Dimida, Simona, Barca, Amilcare, Cancelli, Nadia, De Benedictis, Vincenzo, Raucci, Maria Grazia, Demitri, Christian |
| author_sort | dimida, simona |
| container_start_page | 1 |
| container_title | International Journal of Polymer Science |
| container_volume | 2017 |
| description | <jats:p>Genipin (GN) is a natural molecule extracted from the fruit of<jats:italic> Gardenia jasminoides</jats:italic> Ellis according to modern microbiological processes. Genipin is considered as a favorable cross-linking agent due to its low cytotoxicity compared to widely used cross-linkers; it cross-links compounds with primary amine groups such as proteins, collagen, and chitosan. Chitosan is a biocompatible polymer that is currently studied in bone tissue engineering for its capacity to promote growth and mineral-rich matrix deposition by osteoblasts in culture. In this work, two genipin cross-linked chitosan scaffolds for bone repair and regeneration were prepared with different GN concentrations, and their chemical, physical, and biological properties were explored. Scanning electron microscopy and mechanical tests revealed that nonremarkable changes in morphology, porosity, and mechanical strength of scaffolds are induced by increasing the cross-linking degree. Also, the degradation rate was shown to decrease while increasing the cross-linking degree, with the high cross-linking density of the scaffold disabling the hydrolysis activity. Finally, basic biocompatibility was investigated in vitro, by evaluating proliferation of two human-derived cell lines, namely, the MG63 (human immortalized osteosarcoma) and the hMSCs (human mesenchymal stem cells), as suitable cell models for bone tissue engineering applications of biomaterials.</jats:p> |
| doi_str_mv | 10.1155/2017/8410750 |
| facet_avail | Online, Free |
| finc_class_facet | Chemie und Pharmazie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1NS8yMDE3Lzg0MTA3NTA |
| imprint | Hindawi Limited, 2017 |
| imprint_str_mv | Hindawi Limited, 2017 |
| institution | DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1 |
| issn | 1687-9430, 1687-9422 |
| issn_str_mv | 1687-9430, 1687-9422 |
| language | English |
| last_indexed | 2024-03-01T18:04:03.243Z |
| match_str | dimida2017effectsofgenipinconcentrationoncrosslinkedchitosanscaffoldsforbonetissueengineeringstructuralcharacterizationandevidenceofbiocompatibilityfeatures |
| mega_collection | Hindawi Limited (CrossRef) |
| physical | 1-8 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| publisher | Hindawi Limited |
| record_format | ai |
| recordtype | ai |
| series | International Journal of Polymer Science |
| source_id | 49 |
| spelling | Dimida, Simona Barca, Amilcare Cancelli, Nadia De Benedictis, Vincenzo Raucci, Maria Grazia Demitri, Christian 1687-9422 1687-9430 Hindawi Limited Polymers and Plastics http://dx.doi.org/10.1155/2017/8410750 <jats:p>Genipin (GN) is a natural molecule extracted from the fruit of<jats:italic> Gardenia jasminoides</jats:italic> Ellis according to modern microbiological processes. Genipin is considered as a favorable cross-linking agent due to its low cytotoxicity compared to widely used cross-linkers; it cross-links compounds with primary amine groups such as proteins, collagen, and chitosan. Chitosan is a biocompatible polymer that is currently studied in bone tissue engineering for its capacity to promote growth and mineral-rich matrix deposition by osteoblasts in culture. In this work, two genipin cross-linked chitosan scaffolds for bone repair and regeneration were prepared with different GN concentrations, and their chemical, physical, and biological properties were explored. Scanning electron microscopy and mechanical tests revealed that nonremarkable changes in morphology, porosity, and mechanical strength of scaffolds are induced by increasing the cross-linking degree. Also, the degradation rate was shown to decrease while increasing the cross-linking degree, with the high cross-linking density of the scaffold disabling the hydrolysis activity. Finally, basic biocompatibility was investigated in vitro, by evaluating proliferation of two human-derived cell lines, namely, the MG63 (human immortalized osteosarcoma) and the hMSCs (human mesenchymal stem cells), as suitable cell models for bone tissue engineering applications of biomaterials.</jats:p> Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features International Journal of Polymer Science |
| spellingShingle | Dimida, Simona, Barca, Amilcare, Cancelli, Nadia, De Benedictis, Vincenzo, Raucci, Maria Grazia, Demitri, Christian, International Journal of Polymer Science, Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features, Polymers and Plastics |
| title | Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features |
| title_full | Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features |
| title_fullStr | Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features |
| title_full_unstemmed | Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features |
| title_short | Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features |
| title_sort | effects of genipin concentration on cross-linked chitosan scaffolds for bone tissue engineering: structural characterization and evidence of biocompatibility features |
| title_unstemmed | Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features |
| topic | Polymers and Plastics |
| url | http://dx.doi.org/10.1155/2017/8410750 |