Eintrag weiter verarbeiten
Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
Gespeichert in:
Zeitschriftentitel: | The Scientific World Journal |
---|---|
Personen und Körperschaften: | , , , , |
In: | The Scientific World Journal, 2017, 2017, S. 1-7 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Hindawi Limited
|
Schlagwörter: |
author_facet |
Gul, Rahman Jan, Syed Umer Faridullah, Syed Sherani, Samiullah Jahan, Nusrat Gul, Rahman Jan, Syed Umer Faridullah, Syed Sherani, Samiullah Jahan, Nusrat |
---|---|
author |
Gul, Rahman Jan, Syed Umer Faridullah, Syed Sherani, Samiullah Jahan, Nusrat |
spellingShingle |
Gul, Rahman Jan, Syed Umer Faridullah, Syed Sherani, Samiullah Jahan, Nusrat The Scientific World Journal Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine |
author_sort |
gul, rahman |
spelling |
Gul, Rahman Jan, Syed Umer Faridullah, Syed Sherani, Samiullah Jahan, Nusrat 2356-6140 1537-744X Hindawi Limited General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1155/2017/5873648 <jats:p>The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the<jats:italic> E. intermedia</jats:italic> to prove its uses in Pakistani folk medicines for the treatment of asthma and bronchitis. Antioxidant activity was analyzed by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. Standard methods were used for the identification of cardiac glycosides, phenolic compounds, flavonoids, anthraquinones, and alkaloids. High performance liquid chromatography (HPLC) was used for quantitative purpose of ephedrine alkaloids in<jats:italic> E. intermedia</jats:italic>. The quantitative separation was confirmed on Shimadzu 10AVP column (Shampack) of internal diameter (id) 3.0 mm and 50 mm in length. The extract of the solute in flow rate of 1 ml/min at the wavelength 210 nm and methanolic extract showed the antioxidant activity and powerful oxygen free radicals scavenging activities and the IC50 for the<jats:italic> E. intermedia</jats:italic> plant was near to the reference standard ascorbic acid. The HPLC method was useful for the quantitative purpose of ephedrine (E) and pseudoephedrine (PE) used for 45 samples of one species collected from central habitat in three districts (Ziarat, Shairani, and Kalat) of Balochistan. Results showed that average alkaloid substance in<jats:italic> E. intermedia</jats:italic> was as follows: PE (0.209%, 0.238%, and 0.22%) and E (0.0538%, 0.0666%, and 0.0514%).</jats:p> Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from<i> Ephedra intermedia</i> Indigenous to Balochistan The Scientific World Journal |
doi_str_mv |
10.1155/2017/5873648 |
facet_avail |
Online Free |
format |
ElectronicArticle |
fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1NS8yMDE3LzU4NzM2NDg |
id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1NS8yMDE3LzU4NzM2NDg |
institution |
DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Rs1 DE-Pl11 DE-105 DE-14 DE-Ch1 |
imprint |
Hindawi Limited, 2017 |
imprint_str_mv |
Hindawi Limited, 2017 |
issn |
2356-6140 1537-744X |
issn_str_mv |
2356-6140 1537-744X |
language |
English |
mega_collection |
Hindawi Limited (CrossRef) |
match_str |
gul2017preliminaryphytochemicalscreeningquantitativeanalysisofalkaloidsandantioxidantactivityofcrudeplantextractsfromephedraintermediaindigenoustobalochistan |
publishDateSort |
2017 |
publisher |
Hindawi Limited |
recordtype |
ai |
record_format |
ai |
series |
The Scientific World Journal |
source_id |
49 |
title |
Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan |
title_unstemmed |
Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan |
title_full |
Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan |
title_fullStr |
Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan |
title_full_unstemmed |
Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan |
title_short |
Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan |
title_sort |
preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from<i> ephedra intermedia</i> indigenous to balochistan |
topic |
General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine |
url |
http://dx.doi.org/10.1155/2017/5873648 |
publishDate |
2017 |
physical |
1-7 |
description |
<jats:p>The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the<jats:italic> E. intermedia</jats:italic> to prove its uses in Pakistani folk medicines for the treatment of asthma and bronchitis. Antioxidant activity was analyzed by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. Standard methods were used for the identification of cardiac glycosides, phenolic compounds, flavonoids, anthraquinones, and alkaloids. High performance liquid chromatography (HPLC) was used for quantitative purpose of ephedrine alkaloids in<jats:italic> E. intermedia</jats:italic>. The quantitative separation was confirmed on Shimadzu 10AVP column (Shampack) of internal diameter (id) 3.0 mm and 50 mm in length. The extract of the solute in flow rate of 1 ml/min at the wavelength 210 nm and methanolic extract showed the antioxidant activity and powerful oxygen free radicals scavenging activities and the IC50 for the<jats:italic> E. intermedia</jats:italic> plant was near to the reference standard ascorbic acid. The HPLC method was useful for the quantitative purpose of ephedrine (E) and pseudoephedrine (PE) used for 45 samples of one species collected from central habitat in three districts (Ziarat, Shairani, and Kalat) of Balochistan. Results showed that average alkaloid substance in<jats:italic> E. intermedia</jats:italic> was as follows: PE (0.209%, 0.238%, and 0.22%) and E (0.0538%, 0.0666%, and 0.0514%).</jats:p> |
container_start_page |
1 |
container_title |
The Scientific World Journal |
container_volume |
2017 |
format_de105 |
Article, E-Article |
format_de14 |
Article, E-Article |
format_de15 |
Article, E-Article |
format_de520 |
Article, E-Article |
format_de540 |
Article, E-Article |
format_dech1 |
Article, E-Article |
format_ded117 |
Article, E-Article |
format_degla1 |
E-Article |
format_del152 |
Buch |
format_del189 |
Article, E-Article |
format_dezi4 |
Article |
format_dezwi2 |
Article, E-Article |
format_finc |
Article, E-Article |
format_nrw |
Article, E-Article |
_version_ |
1792346580941013002 |
geogr_code |
not assigned |
last_indexed |
2024-03-01T17:41:39.123Z |
geogr_code_person |
not assigned |
openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Preliminary+Phytochemical+Screening%2C+Quantitative+Analysis+of+Alkaloids%2C+and+Antioxidant+Activity+of+Crude+Plant+Extracts+from+Ephedra+intermedia+Indigenous+to+Balochistan&rft.date=2017-01-01&genre=article&issn=1537-744X&volume=2017&spage=1&epage=7&pages=1-7&jtitle=The+Scientific+World+Journal&atitle=Preliminary+Phytochemical+Screening%2C+Quantitative+Analysis+of+Alkaloids%2C+and+Antioxidant+Activity+of+Crude+Plant+Extracts+from%3Ci%3E+Ephedra+intermedia%3C%2Fi%3E+Indigenous+to+Balochistan&aulast=Jahan&aufirst=Nusrat&rft_id=info%3Adoi%2F10.1155%2F2017%2F5873648&rft.language%5B0%5D=eng |
SOLR | |
_version_ | 1792346580941013002 |
author | Gul, Rahman, Jan, Syed Umer, Faridullah, Syed, Sherani, Samiullah, Jahan, Nusrat |
author_facet | Gul, Rahman, Jan, Syed Umer, Faridullah, Syed, Sherani, Samiullah, Jahan, Nusrat, Gul, Rahman, Jan, Syed Umer, Faridullah, Syed, Sherani, Samiullah, Jahan, Nusrat |
author_sort | gul, rahman |
container_start_page | 1 |
container_title | The Scientific World Journal |
container_volume | 2017 |
description | <jats:p>The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the<jats:italic> E. intermedia</jats:italic> to prove its uses in Pakistani folk medicines for the treatment of asthma and bronchitis. Antioxidant activity was analyzed by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. Standard methods were used for the identification of cardiac glycosides, phenolic compounds, flavonoids, anthraquinones, and alkaloids. High performance liquid chromatography (HPLC) was used for quantitative purpose of ephedrine alkaloids in<jats:italic> E. intermedia</jats:italic>. The quantitative separation was confirmed on Shimadzu 10AVP column (Shampack) of internal diameter (id) 3.0 mm and 50 mm in length. The extract of the solute in flow rate of 1 ml/min at the wavelength 210 nm and methanolic extract showed the antioxidant activity and powerful oxygen free radicals scavenging activities and the IC50 for the<jats:italic> E. intermedia</jats:italic> plant was near to the reference standard ascorbic acid. The HPLC method was useful for the quantitative purpose of ephedrine (E) and pseudoephedrine (PE) used for 45 samples of one species collected from central habitat in three districts (Ziarat, Shairani, and Kalat) of Balochistan. Results showed that average alkaloid substance in<jats:italic> E. intermedia</jats:italic> was as follows: PE (0.209%, 0.238%, and 0.22%) and E (0.0538%, 0.0666%, and 0.0514%).</jats:p> |
doi_str_mv | 10.1155/2017/5873648 |
facet_avail | Online, Free |
format | ElectronicArticle |
format_de105 | Article, E-Article |
format_de14 | Article, E-Article |
format_de15 | Article, E-Article |
format_de520 | Article, E-Article |
format_de540 | Article, E-Article |
format_dech1 | Article, E-Article |
format_ded117 | Article, E-Article |
format_degla1 | E-Article |
format_del152 | Buch |
format_del189 | Article, E-Article |
format_dezi4 | Article |
format_dezwi2 | Article, E-Article |
format_finc | Article, E-Article |
format_nrw | Article, E-Article |
geogr_code | not assigned |
geogr_code_person | not assigned |
id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1NS8yMDE3LzU4NzM2NDg |
imprint | Hindawi Limited, 2017 |
imprint_str_mv | Hindawi Limited, 2017 |
institution | DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1 |
issn | 2356-6140, 1537-744X |
issn_str_mv | 2356-6140, 1537-744X |
language | English |
last_indexed | 2024-03-01T17:41:39.123Z |
match_str | gul2017preliminaryphytochemicalscreeningquantitativeanalysisofalkaloidsandantioxidantactivityofcrudeplantextractsfromephedraintermediaindigenoustobalochistan |
mega_collection | Hindawi Limited (CrossRef) |
physical | 1-7 |
publishDate | 2017 |
publishDateSort | 2017 |
publisher | Hindawi Limited |
record_format | ai |
recordtype | ai |
series | The Scientific World Journal |
source_id | 49 |
spelling | Gul, Rahman Jan, Syed Umer Faridullah, Syed Sherani, Samiullah Jahan, Nusrat 2356-6140 1537-744X Hindawi Limited General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1155/2017/5873648 <jats:p>The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the<jats:italic> E. intermedia</jats:italic> to prove its uses in Pakistani folk medicines for the treatment of asthma and bronchitis. Antioxidant activity was analyzed by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. Standard methods were used for the identification of cardiac glycosides, phenolic compounds, flavonoids, anthraquinones, and alkaloids. High performance liquid chromatography (HPLC) was used for quantitative purpose of ephedrine alkaloids in<jats:italic> E. intermedia</jats:italic>. The quantitative separation was confirmed on Shimadzu 10AVP column (Shampack) of internal diameter (id) 3.0 mm and 50 mm in length. The extract of the solute in flow rate of 1 ml/min at the wavelength 210 nm and methanolic extract showed the antioxidant activity and powerful oxygen free radicals scavenging activities and the IC50 for the<jats:italic> E. intermedia</jats:italic> plant was near to the reference standard ascorbic acid. The HPLC method was useful for the quantitative purpose of ephedrine (E) and pseudoephedrine (PE) used for 45 samples of one species collected from central habitat in three districts (Ziarat, Shairani, and Kalat) of Balochistan. Results showed that average alkaloid substance in<jats:italic> E. intermedia</jats:italic> was as follows: PE (0.209%, 0.238%, and 0.22%) and E (0.0538%, 0.0666%, and 0.0514%).</jats:p> Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from<i> Ephedra intermedia</i> Indigenous to Balochistan The Scientific World Journal |
spellingShingle | Gul, Rahman, Jan, Syed Umer, Faridullah, Syed, Sherani, Samiullah, Jahan, Nusrat, The Scientific World Journal, Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan, General Environmental Science, General Biochemistry, Genetics and Molecular Biology, General Medicine |
title | Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan |
title_full | Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan |
title_fullStr | Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan |
title_full_unstemmed | Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan |
title_short | Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan |
title_sort | preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from<i> ephedra intermedia</i> indigenous to balochistan |
title_unstemmed | Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan |
topic | General Environmental Science, General Biochemistry, Genetics and Molecular Biology, General Medicine |
url | http://dx.doi.org/10.1155/2017/5873648 |