author_facet Gul, Rahman
Jan, Syed Umer
Faridullah, Syed
Sherani, Samiullah
Jahan, Nusrat
Gul, Rahman
Jan, Syed Umer
Faridullah, Syed
Sherani, Samiullah
Jahan, Nusrat
author Gul, Rahman
Jan, Syed Umer
Faridullah, Syed
Sherani, Samiullah
Jahan, Nusrat
spellingShingle Gul, Rahman
Jan, Syed Umer
Faridullah, Syed
Sherani, Samiullah
Jahan, Nusrat
The Scientific World Journal
Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
General Environmental Science
General Biochemistry, Genetics and Molecular Biology
General Medicine
author_sort gul, rahman
spelling Gul, Rahman Jan, Syed Umer Faridullah, Syed Sherani, Samiullah Jahan, Nusrat 2356-6140 1537-744X Hindawi Limited General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1155/2017/5873648 <jats:p>The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the<jats:italic> E. intermedia</jats:italic> to prove its uses in Pakistani folk medicines for the treatment of asthma and bronchitis. Antioxidant activity was analyzed by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. Standard methods were used for the identification of cardiac glycosides, phenolic compounds, flavonoids, anthraquinones, and alkaloids. High performance liquid chromatography (HPLC) was used for quantitative purpose of ephedrine alkaloids in<jats:italic> E. intermedia</jats:italic>. The quantitative separation was confirmed on Shimadzu 10AVP column (Shampack) of internal diameter (id) 3.0 mm and 50 mm in length. The extract of the solute in flow rate of 1 ml/min at the wavelength 210 nm and methanolic extract showed the antioxidant activity and powerful oxygen free radicals scavenging activities and the IC50 for the<jats:italic> E. intermedia</jats:italic> plant was near to the reference standard ascorbic acid. The HPLC method was useful for the quantitative purpose of ephedrine (E) and pseudoephedrine (PE) used for 45 samples of one species collected from central habitat in three districts (Ziarat, Shairani, and Kalat) of Balochistan. Results showed that average alkaloid substance in<jats:italic> E. intermedia</jats:italic> was as follows: PE (0.209%, 0.238%, and 0.22%) and E (0.0538%, 0.0666%, and 0.0514%).</jats:p> Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from<i> Ephedra intermedia</i> Indigenous to Balochistan The Scientific World Journal
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title Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
title_unstemmed Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
title_full Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
title_fullStr Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
title_full_unstemmed Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
title_short Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
title_sort preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from<i> ephedra intermedia</i> indigenous to balochistan
topic General Environmental Science
General Biochemistry, Genetics and Molecular Biology
General Medicine
url http://dx.doi.org/10.1155/2017/5873648
publishDate 2017
physical 1-7
description <jats:p>The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the<jats:italic> E. intermedia</jats:italic> to prove its uses in Pakistani folk medicines for the treatment of asthma and bronchitis. Antioxidant activity was analyzed by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. Standard methods were used for the identification of cardiac glycosides, phenolic compounds, flavonoids, anthraquinones, and alkaloids. High performance liquid chromatography (HPLC) was used for quantitative purpose of ephedrine alkaloids in<jats:italic> E. intermedia</jats:italic>. The quantitative separation was confirmed on Shimadzu 10AVP column (Shampack) of internal diameter (id) 3.0 mm and 50 mm in length. The extract of the solute in flow rate of 1 ml/min at the wavelength 210 nm and methanolic extract showed the antioxidant activity and powerful oxygen free radicals scavenging activities and the IC50 for the<jats:italic> E. intermedia</jats:italic> plant was near to the reference standard ascorbic acid. The HPLC method was useful for the quantitative purpose of ephedrine (E) and pseudoephedrine (PE) used for 45 samples of one species collected from central habitat in three districts (Ziarat, Shairani, and Kalat) of Balochistan. Results showed that average alkaloid substance in<jats:italic> E. intermedia</jats:italic> was as follows: PE (0.209%, 0.238%, and 0.22%) and E (0.0538%, 0.0666%, and 0.0514%).</jats:p>
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spelling Gul, Rahman Jan, Syed Umer Faridullah, Syed Sherani, Samiullah Jahan, Nusrat 2356-6140 1537-744X Hindawi Limited General Environmental Science General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1155/2017/5873648 <jats:p>The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the<jats:italic> E. intermedia</jats:italic> to prove its uses in Pakistani folk medicines for the treatment of asthma and bronchitis. Antioxidant activity was analyzed by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. Standard methods were used for the identification of cardiac glycosides, phenolic compounds, flavonoids, anthraquinones, and alkaloids. High performance liquid chromatography (HPLC) was used for quantitative purpose of ephedrine alkaloids in<jats:italic> E. intermedia</jats:italic>. The quantitative separation was confirmed on Shimadzu 10AVP column (Shampack) of internal diameter (id) 3.0 mm and 50 mm in length. The extract of the solute in flow rate of 1 ml/min at the wavelength 210 nm and methanolic extract showed the antioxidant activity and powerful oxygen free radicals scavenging activities and the IC50 for the<jats:italic> E. intermedia</jats:italic> plant was near to the reference standard ascorbic acid. The HPLC method was useful for the quantitative purpose of ephedrine (E) and pseudoephedrine (PE) used for 45 samples of one species collected from central habitat in three districts (Ziarat, Shairani, and Kalat) of Balochistan. Results showed that average alkaloid substance in<jats:italic> E. intermedia</jats:italic> was as follows: PE (0.209%, 0.238%, and 0.22%) and E (0.0538%, 0.0666%, and 0.0514%).</jats:p> Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from<i> Ephedra intermedia</i> Indigenous to Balochistan The Scientific World Journal
spellingShingle Gul, Rahman, Jan, Syed Umer, Faridullah, Syed, Sherani, Samiullah, Jahan, Nusrat, The Scientific World Journal, Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan, General Environmental Science, General Biochemistry, Genetics and Molecular Biology, General Medicine
title Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
title_full Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
title_fullStr Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
title_full_unstemmed Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
title_short Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
title_sort preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from<i> ephedra intermedia</i> indigenous to balochistan
title_unstemmed Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan
topic General Environmental Science, General Biochemistry, Genetics and Molecular Biology, General Medicine
url http://dx.doi.org/10.1155/2017/5873648