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miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT
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| Zeitschriftentitel: | BioMed Research International |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , |
| In: | BioMed Research International, 2016, 2016, S. 1-11 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Hindawi Limited
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| Schlagwörter: |
| author_facet |
Wang, Bin Lv, Kexing Chen, Weixiong Zhao, Jing Luo, Jie Wu, Jianhui Li, Zenghong Qin, Hao Wong, Thian-Sze Yang, Weiqiang Fu, Qing-Ling Lei, Wenbin Wang, Bin Lv, Kexing Chen, Weixiong Zhao, Jing Luo, Jie Wu, Jianhui Li, Zenghong Qin, Hao Wong, Thian-Sze Yang, Weiqiang Fu, Qing-Ling Lei, Wenbin |
|---|---|
| author |
Wang, Bin Lv, Kexing Chen, Weixiong Zhao, Jing Luo, Jie Wu, Jianhui Li, Zenghong Qin, Hao Wong, Thian-Sze Yang, Weiqiang Fu, Qing-Ling Lei, Wenbin |
| spellingShingle |
Wang, Bin Lv, Kexing Chen, Weixiong Zhao, Jing Luo, Jie Wu, Jianhui Li, Zenghong Qin, Hao Wong, Thian-Sze Yang, Weiqiang Fu, Qing-Ling Lei, Wenbin BioMed Research International miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine |
| author_sort |
wang, bin |
| spelling |
Wang, Bin Lv, Kexing Chen, Weixiong Zhao, Jing Luo, Jie Wu, Jianhui Li, Zenghong Qin, Hao Wong, Thian-Sze Yang, Weiqiang Fu, Qing-Ling Lei, Wenbin 2314-6133 2314-6141 Hindawi Limited General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1155/2016/9652789 <jats:p>Previous studies have found that miR-375 and miR-205 were significantly dysregulated in laryngeal squamous cell carcinoma, which contributed to the invasion and migration of LSCC. However, the mechanisms of miR-375 and miR-205 regulating the invasion and migration of LSCC remain unknown. qRT-PCR was performed in 40 pairs of tissue samples to investigate the expression of miR-375 and miR-205 in LSCC and paracarcinoma tissues. To investigate whether or not miR-375 and miR-205 regulated the invasion and migration of LSCC synergistically via AKT-mediated epithelial-mesenchymal transition, miR-375 mimic and miR-205 inhibitor were transfected into SNU899 cells and miR-375 inhibitor and miR-205 mimic were transfected into SNU899 cells, respectively, with or without AKT inhibitor. Then the expressions of miR-375 and miR-205 were validated by qRT-PCR, cell migration and invasion were determined by wound healing assay and transwell invasive assay, and western blot analysis was performed to detect the expression of related proteins. Our results showed that miR-375 and miR-205 regulated the invasion and migration of LSCC via AKT-mediated EMT synergistically. In conclusion, our findings provided not only new information about the molecular mechanism of miRNAs regulating invasion and migration of LSCC, but also a theoretical principle for potential targeting therapy of laryngeal squamous carcinoma.</jats:p> miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT BioMed Research International |
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| title |
miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT |
| title_unstemmed |
miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT |
| title_full |
miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT |
| title_fullStr |
miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT |
| title_full_unstemmed |
miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT |
| title_short |
miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT |
| title_sort |
mir-375 and mir-205 regulate the invasion and migration of laryngeal squamous cell carcinoma synergistically via akt-mediated emt |
| topic |
General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine |
| url |
http://dx.doi.org/10.1155/2016/9652789 |
| publishDate |
2016 |
| physical |
1-11 |
| description |
<jats:p>Previous studies have found that miR-375 and miR-205 were significantly dysregulated in laryngeal squamous cell carcinoma, which contributed to the invasion and migration of LSCC. However, the mechanisms of miR-375 and miR-205 regulating the invasion and migration of LSCC remain unknown. qRT-PCR was performed in 40 pairs of tissue samples to investigate the expression of miR-375 and miR-205 in LSCC and paracarcinoma tissues. To investigate whether or not miR-375 and miR-205 regulated the invasion and migration of LSCC synergistically via AKT-mediated epithelial-mesenchymal transition, miR-375 mimic and miR-205 inhibitor were transfected into SNU899 cells and miR-375 inhibitor and miR-205 mimic were transfected into SNU899 cells, respectively, with or without AKT inhibitor. Then the expressions of miR-375 and miR-205 were validated by qRT-PCR, cell migration and invasion were determined by wound healing assay and transwell invasive assay, and western blot analysis was performed to detect the expression of related proteins. Our results showed that miR-375 and miR-205 regulated the invasion and migration of LSCC via AKT-mediated EMT synergistically. In conclusion, our findings provided not only new information about the molecular mechanism of miRNAs regulating invasion and migration of LSCC, but also a theoretical principle for potential targeting therapy of laryngeal squamous carcinoma.</jats:p> |
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| author | Wang, Bin, Lv, Kexing, Chen, Weixiong, Zhao, Jing, Luo, Jie, Wu, Jianhui, Li, Zenghong, Qin, Hao, Wong, Thian-Sze, Yang, Weiqiang, Fu, Qing-Ling, Lei, Wenbin |
| author_facet | Wang, Bin, Lv, Kexing, Chen, Weixiong, Zhao, Jing, Luo, Jie, Wu, Jianhui, Li, Zenghong, Qin, Hao, Wong, Thian-Sze, Yang, Weiqiang, Fu, Qing-Ling, Lei, Wenbin, Wang, Bin, Lv, Kexing, Chen, Weixiong, Zhao, Jing, Luo, Jie, Wu, Jianhui, Li, Zenghong, Qin, Hao, Wong, Thian-Sze, Yang, Weiqiang, Fu, Qing-Ling, Lei, Wenbin |
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| description | <jats:p>Previous studies have found that miR-375 and miR-205 were significantly dysregulated in laryngeal squamous cell carcinoma, which contributed to the invasion and migration of LSCC. However, the mechanisms of miR-375 and miR-205 regulating the invasion and migration of LSCC remain unknown. qRT-PCR was performed in 40 pairs of tissue samples to investigate the expression of miR-375 and miR-205 in LSCC and paracarcinoma tissues. To investigate whether or not miR-375 and miR-205 regulated the invasion and migration of LSCC synergistically via AKT-mediated epithelial-mesenchymal transition, miR-375 mimic and miR-205 inhibitor were transfected into SNU899 cells and miR-375 inhibitor and miR-205 mimic were transfected into SNU899 cells, respectively, with or without AKT inhibitor. Then the expressions of miR-375 and miR-205 were validated by qRT-PCR, cell migration and invasion were determined by wound healing assay and transwell invasive assay, and western blot analysis was performed to detect the expression of related proteins. Our results showed that miR-375 and miR-205 regulated the invasion and migration of LSCC via AKT-mediated EMT synergistically. In conclusion, our findings provided not only new information about the molecular mechanism of miRNAs regulating invasion and migration of LSCC, but also a theoretical principle for potential targeting therapy of laryngeal squamous carcinoma.</jats:p> |
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| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14 |
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| spelling | Wang, Bin Lv, Kexing Chen, Weixiong Zhao, Jing Luo, Jie Wu, Jianhui Li, Zenghong Qin, Hao Wong, Thian-Sze Yang, Weiqiang Fu, Qing-Ling Lei, Wenbin 2314-6133 2314-6141 Hindawi Limited General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1155/2016/9652789 <jats:p>Previous studies have found that miR-375 and miR-205 were significantly dysregulated in laryngeal squamous cell carcinoma, which contributed to the invasion and migration of LSCC. However, the mechanisms of miR-375 and miR-205 regulating the invasion and migration of LSCC remain unknown. qRT-PCR was performed in 40 pairs of tissue samples to investigate the expression of miR-375 and miR-205 in LSCC and paracarcinoma tissues. To investigate whether or not miR-375 and miR-205 regulated the invasion and migration of LSCC synergistically via AKT-mediated epithelial-mesenchymal transition, miR-375 mimic and miR-205 inhibitor were transfected into SNU899 cells and miR-375 inhibitor and miR-205 mimic were transfected into SNU899 cells, respectively, with or without AKT inhibitor. Then the expressions of miR-375 and miR-205 were validated by qRT-PCR, cell migration and invasion were determined by wound healing assay and transwell invasive assay, and western blot analysis was performed to detect the expression of related proteins. Our results showed that miR-375 and miR-205 regulated the invasion and migration of LSCC via AKT-mediated EMT synergistically. In conclusion, our findings provided not only new information about the molecular mechanism of miRNAs regulating invasion and migration of LSCC, but also a theoretical principle for potential targeting therapy of laryngeal squamous carcinoma.</jats:p> miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT BioMed Research International |
| spellingShingle | Wang, Bin, Lv, Kexing, Chen, Weixiong, Zhao, Jing, Luo, Jie, Wu, Jianhui, Li, Zenghong, Qin, Hao, Wong, Thian-Sze, Yang, Weiqiang, Fu, Qing-Ling, Lei, Wenbin, BioMed Research International, miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT, General Immunology and Microbiology, General Biochemistry, Genetics and Molecular Biology, General Medicine |
| title | miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT |
| title_full | miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT |
| title_fullStr | miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT |
| title_full_unstemmed | miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT |
| title_short | miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT |
| title_sort | mir-375 and mir-205 regulate the invasion and migration of laryngeal squamous cell carcinoma synergistically via akt-mediated emt |
| title_unstemmed | miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT |
| topic | General Immunology and Microbiology, General Biochemistry, Genetics and Molecular Biology, General Medicine |
| url | http://dx.doi.org/10.1155/2016/9652789 |