Eintrag weiter verarbeiten
Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901
Gespeichert in:
| Zeitschriftentitel: | Stem Cells International |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Stem Cells International, 2016, 2016, S. 1-12 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Hindawi Limited
|
| Schlagwörter: |
| author_facet |
Ai, Zhiying Shao, Jingjing Shi, Xinglong Yu, Mengying Wu, Yongyan Du, Juan Zhang, Yong Guo, Zekun Ai, Zhiying Shao, Jingjing Shi, Xinglong Yu, Mengying Wu, Yongyan Du, Juan Zhang, Yong Guo, Zekun |
|---|---|
| author |
Ai, Zhiying Shao, Jingjing Shi, Xinglong Yu, Mengying Wu, Yongyan Du, Juan Zhang, Yong Guo, Zekun |
| spellingShingle |
Ai, Zhiying Shao, Jingjing Shi, Xinglong Yu, Mengying Wu, Yongyan Du, Juan Zhang, Yong Guo, Zekun Stem Cells International Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 Cell Biology Molecular Biology |
| author_sort |
ai, zhiying |
| spelling |
Ai, Zhiying Shao, Jingjing Shi, Xinglong Yu, Mengying Wu, Yongyan Du, Juan Zhang, Yong Guo, Zekun 1687-966X 1687-9678 Hindawi Limited Cell Biology Molecular Biology http://dx.doi.org/10.1155/2016/1792573 <jats:p>Embryonic stem cells (ESCs) have the ability to grow indefinitely and retain their pluripotency in culture, and this self-renewal capacity is governed by several crucial molecular pathways controlled by specific regulatory genes and epigenetic modifications. It is reported that multiple epigenetic regulators, such as miRNA and pluripotency factors, can be tightly integrated into molecular pathways and cooperate to maintain self-renewal of ESCs. However, mouse ESCs in serum-containing medium seem to be heterogeneous due to the self-activating differentiation signal of MEK/ERK. Thus, to seek for the crucial miRNA and key regulatory genes that establish ESC properties in MEK/ERK pathway, we performed microarray analysis and small RNA deep-sequencing of J1 mESCs treated with or without PD0325901 (PD), a well-known inhibitor of MEK/ERK signal pathway, followed by verification of western blot analysis and quantitative real-time PCR verification; we found that PD regulated the transcript expressions related to self-renewal and differentiation and antagonized the action of retinoic acid- (RA-) induced differentiation. Moreover, PD can significantly modulate the expressions of multiple miRNAs that have crucial functions in ESC development. Overall, our results demonstrate that PD could enhance ESC self-renewal capacity both by key regulatory genes and ES cell-specific miRNA, which in turn influences ESC self-renewal and cellular differentiation.</jats:p> Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 Stem Cells International |
| doi_str_mv |
10.1155/2016/1792573 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1NS8yMDE2LzE3OTI1NzM |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1NS8yMDE2LzE3OTI1NzM |
| institution |
DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 |
| imprint |
Hindawi Limited, 2016 |
| imprint_str_mv |
Hindawi Limited, 2016 |
| issn |
1687-966X 1687-9678 |
| issn_str_mv |
1687-966X 1687-9678 |
| language |
English |
| mega_collection |
Hindawi Limited (CrossRef) |
| match_str |
ai2016maintenanceofselfrenewalandpluripotencyinj1mouseembryonicstemcellsthroughregulatingtranscriptionfactorandmicrornaexpressioninducedbypd0325901 |
| publishDateSort |
2016 |
| publisher |
Hindawi Limited |
| recordtype |
ai |
| record_format |
ai |
| series |
Stem Cells International |
| source_id |
49 |
| title |
Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_unstemmed |
Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_full |
Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_fullStr |
Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_full_unstemmed |
Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_short |
Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_sort |
maintenance of self-renewal and pluripotency in j1 mouse embryonic stem cells through regulating transcription factor and microrna expression induced by pd0325901 |
| topic |
Cell Biology Molecular Biology |
| url |
http://dx.doi.org/10.1155/2016/1792573 |
| publishDate |
2016 |
| physical |
1-12 |
| description |
<jats:p>Embryonic stem cells (ESCs) have the ability to grow indefinitely and retain their pluripotency in culture, and this self-renewal capacity is governed by several crucial molecular pathways controlled by specific regulatory genes and epigenetic modifications. It is reported that multiple epigenetic regulators, such as miRNA and pluripotency factors, can be tightly integrated into molecular pathways and cooperate to maintain self-renewal of ESCs. However, mouse ESCs in serum-containing medium seem to be heterogeneous due to the self-activating differentiation signal of MEK/ERK. Thus, to seek for the crucial miRNA and key regulatory genes that establish ESC properties in MEK/ERK pathway, we performed microarray analysis and small RNA deep-sequencing of J1 mESCs treated with or without PD0325901 (PD), a well-known inhibitor of MEK/ERK signal pathway, followed by verification of western blot analysis and quantitative real-time PCR verification; we found that PD regulated the transcript expressions related to self-renewal and differentiation and antagonized the action of retinoic acid- (RA-) induced differentiation. Moreover, PD can significantly modulate the expressions of multiple miRNAs that have crucial functions in ESC development. Overall, our results demonstrate that PD could enhance ESC self-renewal capacity both by key regulatory genes and ES cell-specific miRNA, which in turn influences ESC self-renewal and cellular differentiation.</jats:p> |
| container_start_page |
1 |
| container_title |
Stem Cells International |
| container_volume |
2016 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792333030178684928 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T14:05:32.061Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Maintenance+of+Self-Renewal+and+Pluripotency+in+J1+Mouse+Embryonic+Stem+Cells+through+Regulating+Transcription+Factor+and+MicroRNA+Expression+Induced+by+PD0325901&rft.date=2016-01-01&genre=article&issn=1687-9678&volume=2016&spage=1&epage=12&pages=1-12&jtitle=Stem+Cells+International&atitle=Maintenance+of+Self-Renewal+and+Pluripotency+in+J1+Mouse+Embryonic+Stem+Cells+through+Regulating+Transcription+Factor+and+MicroRNA+Expression+Induced+by+PD0325901&aulast=Guo&aufirst=Zekun&rft_id=info%3Adoi%2F10.1155%2F2016%2F1792573&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792333030178684928 |
| author | Ai, Zhiying, Shao, Jingjing, Shi, Xinglong, Yu, Mengying, Wu, Yongyan, Du, Juan, Zhang, Yong, Guo, Zekun |
| author_facet | Ai, Zhiying, Shao, Jingjing, Shi, Xinglong, Yu, Mengying, Wu, Yongyan, Du, Juan, Zhang, Yong, Guo, Zekun, Ai, Zhiying, Shao, Jingjing, Shi, Xinglong, Yu, Mengying, Wu, Yongyan, Du, Juan, Zhang, Yong, Guo, Zekun |
| author_sort | ai, zhiying |
| container_start_page | 1 |
| container_title | Stem Cells International |
| container_volume | 2016 |
| description | <jats:p>Embryonic stem cells (ESCs) have the ability to grow indefinitely and retain their pluripotency in culture, and this self-renewal capacity is governed by several crucial molecular pathways controlled by specific regulatory genes and epigenetic modifications. It is reported that multiple epigenetic regulators, such as miRNA and pluripotency factors, can be tightly integrated into molecular pathways and cooperate to maintain self-renewal of ESCs. However, mouse ESCs in serum-containing medium seem to be heterogeneous due to the self-activating differentiation signal of MEK/ERK. Thus, to seek for the crucial miRNA and key regulatory genes that establish ESC properties in MEK/ERK pathway, we performed microarray analysis and small RNA deep-sequencing of J1 mESCs treated with or without PD0325901 (PD), a well-known inhibitor of MEK/ERK signal pathway, followed by verification of western blot analysis and quantitative real-time PCR verification; we found that PD regulated the transcript expressions related to self-renewal and differentiation and antagonized the action of retinoic acid- (RA-) induced differentiation. Moreover, PD can significantly modulate the expressions of multiple miRNAs that have crucial functions in ESC development. Overall, our results demonstrate that PD could enhance ESC self-renewal capacity both by key regulatory genes and ES cell-specific miRNA, which in turn influences ESC self-renewal and cellular differentiation.</jats:p> |
| doi_str_mv | 10.1155/2016/1792573 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTE1NS8yMDE2LzE3OTI1NzM |
| imprint | Hindawi Limited, 2016 |
| imprint_str_mv | Hindawi Limited, 2016 |
| institution | DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1 |
| issn | 1687-966X, 1687-9678 |
| issn_str_mv | 1687-966X, 1687-9678 |
| language | English |
| last_indexed | 2024-03-01T14:05:32.061Z |
| match_str | ai2016maintenanceofselfrenewalandpluripotencyinj1mouseembryonicstemcellsthroughregulatingtranscriptionfactorandmicrornaexpressioninducedbypd0325901 |
| mega_collection | Hindawi Limited (CrossRef) |
| physical | 1-12 |
| publishDate | 2016 |
| publishDateSort | 2016 |
| publisher | Hindawi Limited |
| record_format | ai |
| recordtype | ai |
| series | Stem Cells International |
| source_id | 49 |
| spelling | Ai, Zhiying Shao, Jingjing Shi, Xinglong Yu, Mengying Wu, Yongyan Du, Juan Zhang, Yong Guo, Zekun 1687-966X 1687-9678 Hindawi Limited Cell Biology Molecular Biology http://dx.doi.org/10.1155/2016/1792573 <jats:p>Embryonic stem cells (ESCs) have the ability to grow indefinitely and retain their pluripotency in culture, and this self-renewal capacity is governed by several crucial molecular pathways controlled by specific regulatory genes and epigenetic modifications. It is reported that multiple epigenetic regulators, such as miRNA and pluripotency factors, can be tightly integrated into molecular pathways and cooperate to maintain self-renewal of ESCs. However, mouse ESCs in serum-containing medium seem to be heterogeneous due to the self-activating differentiation signal of MEK/ERK. Thus, to seek for the crucial miRNA and key regulatory genes that establish ESC properties in MEK/ERK pathway, we performed microarray analysis and small RNA deep-sequencing of J1 mESCs treated with or without PD0325901 (PD), a well-known inhibitor of MEK/ERK signal pathway, followed by verification of western blot analysis and quantitative real-time PCR verification; we found that PD regulated the transcript expressions related to self-renewal and differentiation and antagonized the action of retinoic acid- (RA-) induced differentiation. Moreover, PD can significantly modulate the expressions of multiple miRNAs that have crucial functions in ESC development. Overall, our results demonstrate that PD could enhance ESC self-renewal capacity both by key regulatory genes and ES cell-specific miRNA, which in turn influences ESC self-renewal and cellular differentiation.</jats:p> Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 Stem Cells International |
| spellingShingle | Ai, Zhiying, Shao, Jingjing, Shi, Xinglong, Yu, Mengying, Wu, Yongyan, Du, Juan, Zhang, Yong, Guo, Zekun, Stem Cells International, Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901, Cell Biology, Molecular Biology |
| title | Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_full | Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_fullStr | Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_full_unstemmed | Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_short | Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_sort | maintenance of self-renewal and pluripotency in j1 mouse embryonic stem cells through regulating transcription factor and microrna expression induced by pd0325901 |
| title_unstemmed | Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| topic | Cell Biology, Molecular Biology |
| url | http://dx.doi.org/10.1155/2016/1792573 |