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Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation
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| Zeitschriftentitel: | Evidence-Based Complementary and Alternative Medicine |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Evidence-Based Complementary and Alternative Medicine, 2015, 2015, S. 1-10 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Hindawi Limited
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| Schlagwörter: |
| author_facet |
Jiao, Yi Fan, Yi-Fei Wang, Yu-Ling Zhang, Jun-Yan Chen, Shuo Chen, Zhi-Wu Jiao, Yi Fan, Yi-Fei Wang, Yu-Ling Zhang, Jun-Yan Chen, Shuo Chen, Zhi-Wu |
|---|---|
| author |
Jiao, Yi Fan, Yi-Fei Wang, Yu-Ling Zhang, Jun-Yan Chen, Shuo Chen, Zhi-Wu |
| spellingShingle |
Jiao, Yi Fan, Yi-Fei Wang, Yu-Ling Zhang, Jun-Yan Chen, Shuo Chen, Zhi-Wu Evidence-Based Complementary and Alternative Medicine Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation Complementary and alternative medicine |
| author_sort |
jiao, yi |
| spelling |
Jiao, Yi Fan, Yi-Fei Wang, Yu-Ling Zhang, Jun-Yan Chen, Shuo Chen, Zhi-Wu 1741-427X 1741-4288 Hindawi Limited Complementary and alternative medicine http://dx.doi.org/10.1155/2015/863531 <jats:p>Many flavonoids have cardioprotection against myocardial ischemia/reperfusion (I/R) injury. Total flavones from<jats:italic>Rhododendron simsii</jats:italic>Planch flower (TFR) can protect myocardial ischemic injuries. However, its protective mechanism is still unknown. The present study was designed to investigate the mechanism of TFR on myocardial I/R and anoxia/reoxygenation (A/R) injuries. Rat model of myocardial I/R injury was made, and myocardial infarction was determined. A/R injury was induced in cultured rat cardiomyocytes; cellular damage was evaluated by measuring cell viability, LDH and cTnT releases, and MDA content. Expressions of ROCK<jats:sub>1</jats:sub>and ROCK<jats:sub>2</jats:sub>protein were examined by Western blot analysis, and K<jats:sup>+</jats:sup>currents were recorded by using whole-cell patch clamp technique. TFR 20~80 mg/kg markedly reduced I/R-induced myocardial infarction. TFR 3.7~300 mg/L significantly inhibited A/R-induced reduction of cell viability, LDH and cTnT releases, and MDA production. Exposure to A/R significantly increased ROCK<jats:sub>1</jats:sub>and ROCK<jats:sub>2</jats:sub>expressions in rat cardiomyocytes, but TFR 33.3~300 mg/L obviously inhibited this increase. 300 mg/L TFR significantly augmented inward rectifier K<jats:sup>+</jats:sup>current and other K<jats:sup>+</jats:sup>currents in rat cardiomyocytes. These results indicate that TFR has a protective effect on rat cardiomyocytes A/R damage, and the protective mechanism may be engaged with the inhibition of ROCK<jats:sub>1</jats:sub>and ROCK<jats:sub>2</jats:sub>and activation of K<jats:sup>+</jats:sup>channels.</jats:p> Protective Effect and Mechanism of Total Flavones from<i>Rhododendron simsii</i>Planch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation Evidence-Based Complementary and Alternative Medicine |
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| title |
Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation |
| title_unstemmed |
Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation |
| title_full |
Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation |
| title_fullStr |
Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation |
| title_full_unstemmed |
Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation |
| title_short |
Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation |
| title_sort |
protective effect and mechanism of total flavones from<i>rhododendron simsii</i>planch flower on cultured rat cardiomyocytes with anoxia and reoxygenation |
| topic |
Complementary and alternative medicine |
| url |
http://dx.doi.org/10.1155/2015/863531 |
| publishDate |
2015 |
| physical |
1-10 |
| description |
<jats:p>Many flavonoids have cardioprotection against myocardial ischemia/reperfusion (I/R) injury. Total flavones from<jats:italic>Rhododendron simsii</jats:italic>Planch flower (TFR) can protect myocardial ischemic injuries. However, its protective mechanism is still unknown. The present study was designed to investigate the mechanism of TFR on myocardial I/R and anoxia/reoxygenation (A/R) injuries. Rat model of myocardial I/R injury was made, and myocardial infarction was determined. A/R injury was induced in cultured rat cardiomyocytes; cellular damage was evaluated by measuring cell viability, LDH and cTnT releases, and MDA content. Expressions of ROCK<jats:sub>1</jats:sub>and ROCK<jats:sub>2</jats:sub>protein were examined by Western blot analysis, and K<jats:sup>+</jats:sup>currents were recorded by using whole-cell patch clamp technique. TFR 20~80 mg/kg markedly reduced I/R-induced myocardial infarction. TFR 3.7~300 mg/L significantly inhibited A/R-induced reduction of cell viability, LDH and cTnT releases, and MDA production. Exposure to A/R significantly increased ROCK<jats:sub>1</jats:sub>and ROCK<jats:sub>2</jats:sub>expressions in rat cardiomyocytes, but TFR 33.3~300 mg/L obviously inhibited this increase. 300 mg/L TFR significantly augmented inward rectifier K<jats:sup>+</jats:sup>current and other K<jats:sup>+</jats:sup>currents in rat cardiomyocytes. These results indicate that TFR has a protective effect on rat cardiomyocytes A/R damage, and the protective mechanism may be engaged with the inhibition of ROCK<jats:sub>1</jats:sub>and ROCK<jats:sub>2</jats:sub>and activation of K<jats:sup>+</jats:sup>channels.</jats:p> |
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| author | Jiao, Yi, Fan, Yi-Fei, Wang, Yu-Ling, Zhang, Jun-Yan, Chen, Shuo, Chen, Zhi-Wu |
| author_facet | Jiao, Yi, Fan, Yi-Fei, Wang, Yu-Ling, Zhang, Jun-Yan, Chen, Shuo, Chen, Zhi-Wu, Jiao, Yi, Fan, Yi-Fei, Wang, Yu-Ling, Zhang, Jun-Yan, Chen, Shuo, Chen, Zhi-Wu |
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| description | <jats:p>Many flavonoids have cardioprotection against myocardial ischemia/reperfusion (I/R) injury. Total flavones from<jats:italic>Rhododendron simsii</jats:italic>Planch flower (TFR) can protect myocardial ischemic injuries. However, its protective mechanism is still unknown. The present study was designed to investigate the mechanism of TFR on myocardial I/R and anoxia/reoxygenation (A/R) injuries. Rat model of myocardial I/R injury was made, and myocardial infarction was determined. A/R injury was induced in cultured rat cardiomyocytes; cellular damage was evaluated by measuring cell viability, LDH and cTnT releases, and MDA content. Expressions of ROCK<jats:sub>1</jats:sub>and ROCK<jats:sub>2</jats:sub>protein were examined by Western blot analysis, and K<jats:sup>+</jats:sup>currents were recorded by using whole-cell patch clamp technique. TFR 20~80 mg/kg markedly reduced I/R-induced myocardial infarction. TFR 3.7~300 mg/L significantly inhibited A/R-induced reduction of cell viability, LDH and cTnT releases, and MDA production. Exposure to A/R significantly increased ROCK<jats:sub>1</jats:sub>and ROCK<jats:sub>2</jats:sub>expressions in rat cardiomyocytes, but TFR 33.3~300 mg/L obviously inhibited this increase. 300 mg/L TFR significantly augmented inward rectifier K<jats:sup>+</jats:sup>current and other K<jats:sup>+</jats:sup>currents in rat cardiomyocytes. These results indicate that TFR has a protective effect on rat cardiomyocytes A/R damage, and the protective mechanism may be engaged with the inhibition of ROCK<jats:sub>1</jats:sub>and ROCK<jats:sub>2</jats:sub>and activation of K<jats:sup>+</jats:sup>channels.</jats:p> |
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| spelling | Jiao, Yi Fan, Yi-Fei Wang, Yu-Ling Zhang, Jun-Yan Chen, Shuo Chen, Zhi-Wu 1741-427X 1741-4288 Hindawi Limited Complementary and alternative medicine http://dx.doi.org/10.1155/2015/863531 <jats:p>Many flavonoids have cardioprotection against myocardial ischemia/reperfusion (I/R) injury. Total flavones from<jats:italic>Rhododendron simsii</jats:italic>Planch flower (TFR) can protect myocardial ischemic injuries. However, its protective mechanism is still unknown. The present study was designed to investigate the mechanism of TFR on myocardial I/R and anoxia/reoxygenation (A/R) injuries. Rat model of myocardial I/R injury was made, and myocardial infarction was determined. A/R injury was induced in cultured rat cardiomyocytes; cellular damage was evaluated by measuring cell viability, LDH and cTnT releases, and MDA content. Expressions of ROCK<jats:sub>1</jats:sub>and ROCK<jats:sub>2</jats:sub>protein were examined by Western blot analysis, and K<jats:sup>+</jats:sup>currents were recorded by using whole-cell patch clamp technique. TFR 20~80 mg/kg markedly reduced I/R-induced myocardial infarction. TFR 3.7~300 mg/L significantly inhibited A/R-induced reduction of cell viability, LDH and cTnT releases, and MDA production. Exposure to A/R significantly increased ROCK<jats:sub>1</jats:sub>and ROCK<jats:sub>2</jats:sub>expressions in rat cardiomyocytes, but TFR 33.3~300 mg/L obviously inhibited this increase. 300 mg/L TFR significantly augmented inward rectifier K<jats:sup>+</jats:sup>current and other K<jats:sup>+</jats:sup>currents in rat cardiomyocytes. These results indicate that TFR has a protective effect on rat cardiomyocytes A/R damage, and the protective mechanism may be engaged with the inhibition of ROCK<jats:sub>1</jats:sub>and ROCK<jats:sub>2</jats:sub>and activation of K<jats:sup>+</jats:sup>channels.</jats:p> Protective Effect and Mechanism of Total Flavones from<i>Rhododendron simsii</i>Planch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation Evidence-Based Complementary and Alternative Medicine |
| spellingShingle | Jiao, Yi, Fan, Yi-Fei, Wang, Yu-Ling, Zhang, Jun-Yan, Chen, Shuo, Chen, Zhi-Wu, Evidence-Based Complementary and Alternative Medicine, Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation, Complementary and alternative medicine |
| title | Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation |
| title_full | Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation |
| title_fullStr | Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation |
| title_full_unstemmed | Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation |
| title_short | Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation |
| title_sort | protective effect and mechanism of total flavones from<i>rhododendron simsii</i>planch flower on cultured rat cardiomyocytes with anoxia and reoxygenation |
| title_unstemmed | Protective Effect and Mechanism of Total Flavones fromRhododendron simsiiPlanch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation |
| topic | Complementary and alternative medicine |
| url | http://dx.doi.org/10.1155/2015/863531 |